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Citations for New England Biolabs :
151 - 200 of 2381 citations for Recombinant Human GAB1 Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... coli SHuffle T7 Express cells (NEB), together with GroEL/ES + Trigger factor chaperones in a pACYC vector and spread on an Agar plate with ampicillin and chloramphenicol ...
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bioRxiv - Genomics 2023Quote: ... and digested with T7 endonuclease (NEB). The resulting digestion product was run out on 2% agarose ...
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bioRxiv - Biophysics 2023Quote: ... using T7 RNA polymerase (NEB USA) in an optimized reaction mix ...
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bioRxiv - Biochemistry 2023Quote: ... coli T7 express (New England Biolabs). The transformed cells were grown in 2xYT and induced at 16 °C overnight with 0.3 mM isopropyl-β-thio-galactoside (IPTG) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5µL T7 DNA Ligase (BioLabs #M0318S) in dH2O ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... and T7 ligase (New England Biolabs). The construct library was transformed into ElectroMAX Stbl4 competent cells (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 1x T7 RNA Polymerase buffer (NEB), 5 mM ATP (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... coli T7 express (New England Biolabs) cells ...
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bioRxiv - Cell Biology 2024Quote: ... T7 RNA polymerase (New England BioLabs) was used and ribonucleotide solution mix was doped with 30% Aminoallyl-UTP-Cy5 (Jena Bioscience) ...
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bioRxiv - Cell Biology 2022Quote: Recombinant condensin II holo(WT) at 500 nM was mixed with or without λ protein phosphatase (NEB) at a final concentration of 400 U/µL in 1x NEBuffer for Protein MetalloPhosphatases supplemented with 1 mM MnCl2 and incubated at 30°C for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Cell Biology 2022Quote: Recombinant human ADAMTSL2 constructs where generated by PCR-amplification with the Q5 Hot start high fidelity 2x master mix (NEB) and specific primer pairs to allow for restriction cloning ...
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bioRxiv - Cell Biology 2023Quote: ... and 200 μM each of dATP/dCTP/dTTP at 21°C for 10 min and an additional 15 min incubation with 25 nM recombinant human RPA (a gift from Sarah W. Cai) and 0.03 U/μl T4 DNA polymerase (NEB, #M0203) at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... pRSET-dNOS exon 16 DNA was then transformed into T7 Express lysY/lq E.coli protein expression cells (New England Biolabs #C3013) and plated on LB plus ampicillin (60 µl/ml) ...
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bioRxiv - Microbiology 2021Quote: The CV-B3 Δ116-2C protein used for crystallogenesis was produced in Escherichia coli T7 Iq Express pLysS (New England BioLabs). Cells were grown in TB medium containing 100 µg/mL of ampicillin and 34 µg/mL chloramphenicol at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... a pET-derived vector that is normally used for T7 RNA polymerase-dependent protein expression (AVA421) was linearized by digestion with XbaI (NEB) in a reaction containing 1X CutSmart buffer and 0.5 U/μl XbaI to be used for run-off transcription using T7 RNA polymerase to transcribe the first 26 nucleotides following the T7 promoter sequence to generate the standard model RNA substrate ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Constructs expressing GFP-tagged or HA-tagged Shank3 phospho-mutants were generated using the Gibson Assembly kit (NEB) with the wild-type Shank3 as the template ...
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bioRxiv - Cell Biology 2021Quote: ... SNAP-tagged and Halo-tagged versions of TBK1 constructs were generated by inserting SNAP (pSNAPf [New England Biolabs]) or (Halo [pHaloTag vector ...
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bioRxiv - Genomics 2020Quote: ... T7 transcription templates were transcribed using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs). Reactions were cleaned up using the MEGAClear Transcription Clean-up Kit (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: NSD1/2 WT and different cancer mutants (3.4 μM) were mixed with recombinant H3.1 protein (1 μg) (purchased from NEB) or recombinant H3.1 mononucleosomes in methylation buffer (50 mM Tris/HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Purified sgRNAs targeting tlnrd1 and slc45a2 were pooled separately and complexed with recombinant Cas9 protein (New England Biolabs) in vitro using 300mM KCl buffer for 5 minutes at +37°C ...
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bioRxiv - Microbiology 2021Quote: ... which were incubated with 1 μg/ml full-length S protein with His tag that had been pretreated with or without FXa (P8010L, NEB) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: All anti-tau monoclonal antibodies were generated by the hybridoma approach against recombinant tau aggregates (human full-length tau) encapsulated in the ACM Polymersomes (ACM Biolabs, Singapore) and purified by size exclusion chromatography (SEC ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... WT SUMO3-CXCL17 and variants were expressed as soluble proteins in SHuffle® T7 Competent E.coli (New England Biolabs, Hitchin, UK). In both protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... with Hi-Fi DNA builder (NEB). Primers used to amplify specific regions are described in (Supplementary Table 1) ...
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bioRxiv - Bioengineering 2022Quote: mRNAs were transcribed from DNA templates using T7 RNA polymerase (HiScribe T7 High Yield RNA Synthesis Kit, NEB). To generate base modified mRNA variants ...
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bioRxiv - Cancer Biology 2020Quote: ... in vitro transcription with T7 polymerase was done by T7 high yield RNA systhesis kit (New England Biolabs). Purification of mRNA was done by Qiagen mini column ...
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bioRxiv - Genomics 2020Quote: ... probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs, E2050S) supplemented with 1.3 units/μL RNaseOUT (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 µL of T7 polymerase (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB, Ipswich, MA, USA), 20 U of RNase murine inhibitor (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... were introduced through Gibson Assembly between a T7 promoter and T7 terminator in a pUC19-based vector (NEB), pMZL ...
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bioRxiv - Cell Biology 2024Quote: T7 constructs were ordered from IDT and synthesized using HiScribe T7 High Yield RNA Synthesis Kit (NEB, #E2040S). RNA was purified via RNA extraction ...
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bioRxiv - Cell Biology 2020Quote: ... T7 Endonuclease-1 was purchased from NEB, US (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... The HiScribe Quick T7 kit (NEB, E2050S) was then used for in vitro transcription ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.75 μL T7 polymerase (New England Biolabs), 3mM MgCl2 ...
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bioRxiv - Systems Biology 2020Quote: ... we added 1 μL T7 ligase (NEB) and incubated overnight at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... and T7 DNA Ligase (NEB M0318S/L) with the same cycling conditions as the part vectors.
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bioRxiv - Synthetic Biology 2021Quote: ... 250 U T7 RNA Polymerase (NEB #M0251L) and/or 100 U SP6 RNA Polymerase (NEB #M0207L) ...
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bioRxiv - Plant Biology 2020Quote: ... and T7 Shuffle (New England Biolabs, USA) were used to express Hip1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: T7 endonuclease 1 (#E3321, New England BioLabs) was used to survey for heteroduplexed PCR products as a result of mutagenized target loci ...
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bioRxiv - Bioengineering 2022Quote: ... T7 DNA Ligase (New England Biolabs, M0318L) was added ...
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bioRxiv - Genetics 2022Quote: ... T7 Endonuclease I assay (T7EI; NEB M0302L) or restriction enzyme digestion using Mse I (NEB R0525L) ...
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bioRxiv - Microbiology 2021Quote: ... coli Shuffle T7 Express (New England Biolabs) cells carrying either a control plasmid or a periplasmic tpeV construct were grown overnight with shaking at 37°C in liquid LB medium supplemented with 0.2% glucose and ampicillin ...
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bioRxiv - Microbiology 2021Quote: ... coli Shuffle T7 Express (New England Biolabs) cells carrying either a control plasmid or a periplasmic tpeV construct were grown overnight in liquid LB medium supplemented with 0.2% glucose and ampicillin at 37°C with shaking ...
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bioRxiv - Biochemistry 2019Quote: ... to transform Shuffle T7 Express cells (NEB). Expression and purification was done using a similar method to that described previously (1 ...
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bioRxiv - Developmental Biology 2022Quote: ... 2015 using T7 or SP6 Polymerases (NEB) depending on the 5′ sgRNA sequence ...
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bioRxiv - Evolutionary Biology 2021Quote: ... One microliter of T7 endonuclease Ⅰ (NEB) was added to the sample ...
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bioRxiv - Cell Biology 2022Quote: ... V2.0 from Addgene (62988) via combined ligation (T7 ligase NEB).
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... 1 μl of T7 DNA ligase (NEB), and 1 μl of ATP (NEB) ...