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Citations for New England Biolabs :
451 - 500 of 2381 citations for Recombinant Human GAB1 Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... T7 RNA polymerase mediated in vitro transcription (NEB, Frankfurt, Germany, E2040S) was used for sgRNA-generation from the pSpCas9(BB)-2A-GFP (PX458 ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was transcribed using HiScribe T7 RNA polymerase (New England Biolabs) and purified by denaturing polyacrylamide gel electrophoresis (PAGE) ...
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bioRxiv - Immunology 2020Quote: HiScribe™ T7 ARCA mRNA Kit (with tailing) (New England Biolabs);
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bioRxiv - Genomics 2021Quote: ... The HiScribe T7 High-Yield RNA Synthesis Kit (New England BioLabs) was used according to the company’s recommended instruction but with full substitution of N1-methyl-pseudouridine for uridine and co-transcriptional capping with CleanCap AG (TriLink Biotechnologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... After mixing the annealed DNA template with T7 RNA polymerase (NEB) and the reaction mixture [50 mM MgCl2 ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was transcribed using the manufacturers protocol for HiScribe T7 (NEB). RNA was treated with DNase I (NEB) ...
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bioRxiv - Genomics 2022Quote: ... The three arms were ligated by T7 ligase (NEB, Cat# M0318L), at 25 C overnight ...
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bioRxiv - Genomics 2022Quote: The HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) was used for the in vitro synthesis of RNA transcripts from a FLuc control template ...
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bioRxiv - Molecular Biology 2022Quote: ... using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040). Our standard 10 μL reactions used 0.5 μg of linear plasmid template and an 8:1 ARCA:GTP ratio.
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bioRxiv - Molecular Biology 2022Quote: ... transcribed in vitro with the T7 RNA polymerase (New England Biolabs), and in vitro translated with a Rabbit Reticulocyte Lysate system (Promega).
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bioRxiv - Biophysics 2023Quote: ... Mismatched duplexes were then cleaved by T7 Endonuclease I (NEB M0302S) and cleavage products were detected by gel electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... adding a T7 promoter and an AG initiation sequence (Phusion, NEB): Fw primer ...
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bioRxiv - Genomics 2023Quote: ... Template were first digested with T7 endonuclease I (M0302L, NEB, USA) as suggested in the protocol available on the ONT community website ...
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bioRxiv - Molecular Biology 2023Quote: ... using the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S). Transcribed gRNA was cleaned using the RNA Clean and Concentrator kit (Zymo R1013).
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bioRxiv - Biochemistry 2023Quote: ... with the indicated concentration of T7 RNA polymerase (New England Biolabs) FCE ...
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bioRxiv - Bioengineering 2023Quote: ... and confirmed sequences were transformed into T7 SHuffle (New England Biolabs) competent E ...
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bioRxiv - Immunology 2023Quote: sgRNAs were produced using the transcription T7 high yield kit (NEB) and purified with the EZNA micro-elute RNA clean up kit (Omega Bio-tek) ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... Isolated MBP-tagged VPS26C was purified using Amylose beads (New England Biolabs) and eluted using 20 mM Tris pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... and 30 nM recombinant Cas9 nuclease (NEB, M0386S) at 37°C for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg of recombinant H4 (New England Biolabs) and 100 μM S-adenosyl methionine (SAM ...
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bioRxiv - Cell Biology 2023Quote: ... 100 or 500 units of recombinant CK2 (NEB), 5µM or 15µM silmitaserib (CK2 inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µL 1U recombinant shrimp alkaline phosphatase (NEB) and 2.5 µL 10x CutSmart buffer (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), and 0.1 mg/ml RNase A (QIAGEN)] ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), 0.1 mg/ml RNase A (QIAGEN) ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Microbiology 2020Quote: ... we performed a double digestion with Exonuclease T7 and Exonuclease VII (NEB) (Figure 2-figure supplement 1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Heterozygosity was assessed with T7 endonuclease I cleavage (New England Biolabs, #M0302S) upon direct incubation of 10 μl amplicon ...
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bioRxiv - Neuroscience 2022Quote: In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2021Quote: ... using HiScribe™ T7 High yield RNA Synthesis kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... digestion and then in vitro transcribed using HiScribe T7 Transcription Kit (NEB). The RNA transcripts were extracted using Trizol (Thermo ...
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bioRxiv - Pathology 2019Quote: ... 1 µl of either SP6 or T7 polymerase (New England Biolabs, USA). SP6 reactions were incubated at 40°C for 2 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR products were digested with T7 Endonuclease I (T7E1, NEB M0302S) according to the recommended protocol36 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then used HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) to produce tRNALeu ...
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bioRxiv - Biophysics 2021Quote: ... for ybbR-luci-ybbR and GR-LBD T7 express (New England Biolabs), for HtpG E ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express lysY/Iq (New England Biolabs, Ipswich, MA, USA) and purified by Ni- ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, cat. # C3013). The bacterial cultures were grown at 37 °C to an optical density (OD600 ...
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bioRxiv - Biochemistry 2021Quote: RNAs were transcribed in vitro using T7 RNA Polymerase (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM CTP and 0.6 μl T7 polymerase mix (New England Biolabs.) This target mix was then loaded on to the chip by placing 10 μL of the mix on the sticky side of the PCR film (MicroAmp ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sgRNA was synthesized using HiScribe T7 High Yield RNA Synthesis Kit (NEB). The PCR fragment present the T7 promoter at 5’ end and the sequence from sgRNA of S ...
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bioRxiv - Immunology 2020Quote: ... 1.4 μg PCR products were mixed with 50U T7 RNA polymerase (NEB), 1 U RNase inhibitor (NEB) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cut efficiency of sgRNA was tested by T7 Endonuclease I (NEB #M0302L) assay on the DNA of infected cells ...
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bioRxiv - Biophysics 2021Quote: ... RNA was generated using the HiScribe T7 Quick kit (New England Biolabs) and extracted using trizol and isopropanol precipitation ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR templates for the dsRNA were prepared using T7 Megascript Kit (NEB). S2R+ cells were seeded at the density of 106 cells/ml in serum-free medium and 15 µg/ml of dsRNA was added to the cells ...
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bioRxiv - Cell Biology 2019Quote: ... 4µl (or 200Units) of T3 (or T7) RNA polymerase (NEB cat. M0378S) added and incubated at 37°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... and 100 ng of in vitro transcribed luciferase DNA (NEB T7 HiScribe) was added to the aqueous fractions during isopropanol precipitation (52) ...
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bioRxiv - Microbiology 2020Quote: Escherichia coli BL21 (DE3) pRIL and SHuffle T7 (New England Biolabs, USA) strains were routinely grown in Luria Bertani (LB) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM CTP and 0.6 μl T7 polymerase mix (New England Biolabs)
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... plasmids were transformed into SHuffle T7 Express LysY bacteria (NEB Cat# C3030J) and induced with 0.4 mM of IPTG at 16°C for 24h ...