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Citations for New England Biolabs :
151 - 200 of 624 citations for Mouse Anti Human Papilloma virus type 18 718 67 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Luciferase activity was determined in a luminometer (Optocomp I) by the addition of ∼18 µl of sample to 6-7 µl diluted coelenterazine (for Gluc, NEB), or ∼20 µl of sample to 15-18 µl Fluc substrate (Promega).
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bioRxiv - Genomics 2021Quote: ... and on the other side a primer that targets a region introduced through the library preparation called “Read 1” (18 cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs)) ...
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bioRxiv - Neuroscience 2020Quote: ... either purified directly (insert) or from agarose gel (vector) and ligated (16-18 h, 20-22 °C) with T4 DNA ligase (NEB) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... to the branch point recognition sequence (Nucleotides 18-42) of U2 snRNA and to GAPDH mRNA were added in combination with RNase H (NEB) to NE ...
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bioRxiv - Neuroscience 2022Quote: ... was linearized for 18 hours at 37°C in a 250 μl reaction containing restriction enzyme NheI and CutSmart buffer (New England Biolabs). The linear DNA plasmid was next used to produce complementary RNA (cRNA ...
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bioRxiv - Immunology 2022Quote: ... Sample index PCR: 2 µl tagging PCR product was mixed with 18 µl PCR mix (4 µl 5x phusion HF buffer (NEB); 0.4 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... mutants of UFD1-His6 (deletion of amino acids 2-215) and NPL4 (T590L+F591V point mutations) 18 were generated using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR-enrichment was performed in 18 cycles in ten 10μL reactions per sublibrary with Phusion® HF PCR Mastermix (New England BioLabs) by mixing 5µl 2× Phusion Master mix ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was clarified by centrifugation at 5°C at 18 500 rpm for 30min and loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB1_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: pUC19 was digested using thirteen commercially available Type II restriction enzymes (New England Biolabs). For BamHI ...
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bioRxiv - Bioengineering 2022Quote: ... The template DNA was removed by using DpnI type IIM restriction enzyme (NEB, USA) at 37°C (1 – 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... and/or 10 U of T4 PNK (wild-type or 3’ phosphatase minus, NEB). For RtcB ligation ...
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bioRxiv - Biophysics 2022Quote: ... type IIS enzymes (BsaI, New England Biolabs #R3733, and BsmBI, New England Biolabs #R0739) were used to iteratively concatenate sequence modules ...
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bioRxiv - Developmental Biology 2022Quote: ... along with enzymatic digestion of the wild-type amplicon with enzyme BslI (NEB R0555S). Genotyping dmrt2bumb4required treatment with the enzyme BaeGI (NEB R0708S) ...
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bioRxiv - Biophysics 2023Quote: ... the use of a Type IIs restriction enzyme (BsaI-HF-v2, New England Biolabs) that produces unique 4 bp sticky ends ensures that the three fragments are oriented in a unique way.
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bioRxiv - Microbiology 2023Quote: ... Genes of interest were amplified from wild-type genomic DNA using Phusion Polymerase (NEB), according to the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2024Quote: ... R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs, R0158S) for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... falciparum and human histones (New England Biolabs) (6µg) ...
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bioRxiv - Microbiology 2021Quote: ... 40 U RNase Inhibitor Human Placenta (NEB), 50 pmol Oligo d(T)23 (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... Human CK2 was obtained from NEB (P6010S). IE2-NTD was phosphorylated in buffer provided by the manufacturer which contains 50mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: Recombinant human Casein Kinase 2 (NEB, P6010S) and Protein Kinase A (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... then incubated with either mouse anti-MBP antibodies at a dilution of 1:5000 (New England Biolabs) or 1:2500 mouse anti-GFP antibodies (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... Bound protein was detected by western blot using mouse anti-MBP diluted 1:10,000 (New England Biolabs), fluorescent secondary antibodies (Li-Cor Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... and in vitro bacterial cultures was used as template in reverse transcription reactions using the Murine Moloney Leukemia Virus Reverse Transcriptase (NEB M0253S) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... MWCO 10,000 filters and then digested with 10 µl Tobacco etch virus nuclear-inclusion-a endopeptidase (TEV) (New England Biolabs, Ipswich, MA) overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: Alg2 cDNA was amplified with RT-PCR from the cDNA of wt Oryzias latipes Cab strain stage 18 embryos with Q5® High-Fidelity DNA Polymerase (New England Biolabs) by using primers with BamHI-HF (New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: ... 500ng of RNA from MEFs or 350ng of RNA from BMDMs were reverse transcribed using oligo(dT)18 primer (New England Biolabs, #513165) and SuperScript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA fragments were inserted into PCR-amplified (primers 17-18 in Table S3) and purified pRSET vector fragment by Gibson Assembly (NEB, USA) to add sequence parts necessary for cDNA display generation to the DNA library ...
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bioRxiv - Systems Biology 2021Quote: Rho libraries were amplified using primers MO574 and MO575 (Supplementary file 6) for 6 cycles at an annealing temperature of 66C followed by 18 cycles with no annealing step (NEB Phusion) and then purified with the Monarch PCR kit (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C for 18 hours and the plasmid was extracted using the Monarch Plasmid Miniprep Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR product was denatured and annealed in an 18 μl reaction (15 μl PCR product, 2 μl NEB Buffer2 (10X), 1 μl nuclease-free H2O ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated with 25 μL of containing TF or 18S primers and fluorescent Luna® Universal qPCR Master Mix (New England Biolabs), and qRT-PCR was carried out in triplicate for each sample on the CFX Connect real-time System (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 20 µl of distilled water and mixed with 18 µl gel loading dye (New England Biolabs, Inc.). Samples were resolved in a 10% SDS-polyacrylamide gel ...
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bioRxiv - Biochemistry 2019Quote: ... This assembly was performed using a Type IIs restriction enzyme BsaI (New England Biolabs # R3535) and T4 DNA ligase (New England Biolabs # M0202 ...
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bioRxiv - Genomics 2020Quote: KlenTaq wild type gene was amplified using a high fidelity PCR (Q5 polymerase from NEB) from a stored plasmid ...
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bioRxiv - Biochemistry 2022Quote: ... wild-type CSF3R MIG plasmid and insert were digested with SfiI (New England Biolabs R0123S), ligated overnight with a 3:1 molar ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein coding sequences were ordered synthetically with Part 3b type BsaI overhangs (NEB R3733) and cloned into the entry vector with BsmBI (NEB R0739) ...
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bioRxiv - Systems Biology 2023Quote: ... all wild-type bZIP intermediate plasmids were digested with AvrII (New England Biolabs, Ipswitch, MA) and HindIII-HF (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: pSpy0C2 was cloned using Golden Gate Assembly using the type II restriction enzyme Esp3I (NEB). The MCS-cat fragment and the pUC origin were amplified using OLEC10311/10312 from pSpy1C and OLEC10315/10316 from pUC19-mKate2∼ssrA ...
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bioRxiv - Developmental Biology 2021Quote: ... pFastBac dual vector containing the sequence for the N-terminally 6xHis-tagged human Naa15 and truncated human Naa10 (residues 1-160) sequences was digested using KpnI-HF (NEB) and XmaI (NEB ...
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bioRxiv - Biochemistry 2023Quote: Human HA-DHFR was subcloned from human cDNA to pcDNA4/TO by PCR using Q5 High Fidelity 2X mastermix (NEB). Human FPGS-FLAG and GGH-FLAG were subcloned from cDNA clones MHS6278-202755815 (Horizon Discovery ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... MARCH2 chimeras containing the TM domains from either human MARCH4 or human transferrin receptor (TR) were generated using the NEBuilder HiFi DNA assembly kit (New England Biolabs) and the primers listed in Supplemental Table S2 ...
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bioRxiv - Biophysics 2020Quote: ... from human origin were all purchased from NEB. The H2A/H2B and H3.1/H4 were mixed in 2(H2A/H2B)2:1(H3/H4)4 molar ratio to form octamers.
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant human histone H4 (New England Biolabs # M2504S) was used as a substrate ...
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bioRxiv - Neuroscience 2021Quote: ... One μg of RNA was reverse transcribed in a 20 μl reaction using Moloney Murine Leukemia Virus Reverse Transcriptase (New England Biolabs, Evry, France) and random primers and cDNA obtained amplified using Q5® DNA Polymerase (New England Biolabs ...
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bioRxiv - Zoology 2023Quote: ... One μg of total RNA was reverse transcribed in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Evry, France) and random primers ...
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bioRxiv - Biophysics 2019Quote: ... HIV-1 MAG2A-ΔHBR-EGFP was generated from HIV-1 MAG2A-EGFP by deleting amino acids 18-32 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). pEGFP-N1 and pEYFP-N1 were obtained from Clonetech (Mountainview ...
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bioRxiv - Microbiology 2020Quote: ... pCrPV-1A-DcDV was linearized by inverse PCR with primers 17 and 18 and the linearized plasmid was circularized by blunt end ligation with T4 DNA ligase (New England Biolabs, Ipswich, Massachusetts) according to the manufacturer’s instructions to create pCrPV-1A-DcDV-1A ...
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bioRxiv - Plant Biology 2023Quote: ... The YFP-CESA6 construct was used as a template to prepare 18 YFP-CESA6 mutated constructs using a Q5 Site Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) with the specific primers listed in Supplemental Table S4 ...