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Citations for New England Biolabs :
1901 - 1950 of 6282 citations for 6 Quinolinamine 1 ethyl 1 2 3 4 tetrahydro 2 2 4 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Investigations on regulation of miRNAs in rice reveal [Ca2+]cyt signal transduction regulated miRNAsbioRxiv - Plant Biology 2021Quote: Small RNA libraries were prepared with 2 μg of enriched small RNA using NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) (New England Biolabs) according to the manufacturer’s protocol in biological dupicates ...
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bioRxiv - Genomics 2019Quote: A reference mixture of λ-DNA fragments was prepared by digesting λ DNA to completion with 2 units of AciI (New England Biolabs) per µg of λ DNA (New England Biolabs ...
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bioRxiv - Genetics 2020Quote: ... and the uncut wild-type band was about 340bp and the ali1-2 mutant band was 320 bp following cleavage by the restriction endonuclease HindIII (New England Biolabs; Ipswich, MA). Digestion of the ali1-2 fragments was incomplete and as a result ...
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bioRxiv - Biophysics 2021Quote: ... was incubated with empty capsids purified as above (~2 × 1010 particles) and DNA [300 ng of 50- to 766-bp ladder DNA (New England Biolabs, Ipswich, MA) or linearized 2.8-kb or 6-kb plasmid DNA] ...
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bioRxiv - Cancer Biology 2022Quote: ... to remove the 3’-phosphate group from the uncharged tRNA followed by ligation to 5’-adenylated uniquely barcoded adapters using RNA ligase 2 truncated KQ (New England BioLabs, Cat. # M0351L). The resulting tRNAs were then ligated to a 5’-adaptor ...
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bioRxiv - Immunology 2022Quote: ... Delta and BA.2 spike plasmids were linearized by restriction enzymes and transcribed to mRNA by in vitro T7 RNA polymerase (NEB, Cat # E2060S) as previously described10.
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bioRxiv - Microbiology 2022Quote: ... 100ng of cut vector with a 2-fold molar excess of insert were combined in the Gibson assembly reaction (New England Biolabs, Cat# E5510S), following manufacturer instructions ...
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bioRxiv - Plant Biology 2023Quote: ... by comparison of the extracted DNA stained with ethidium bromide with a molecular weight marker (2-Log DNA ladder, New England Biolabs® Inc.). The DNA samples were stored at −70 °C (18 months for RS and a week for URBC) ...
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bioRxiv - Genomics 2022Quote: ... and the results (Figure 2) suggested that a combination of the rare cutter HindIII and the frequent cutter Sau3AI (New England Biolabs, NEB, Ipswich, MA) would provide restriction fragments suitable for ddRAD-Seq (150-700 bp).
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 2 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was performed with 2 μl of the reverse transcription positive reactions and Phusion High Fidelity DNA polymerase (NEB, Ipswich, MA) following manufacturer’s protocols on an Applied Biosystems 2720 Thermal Cycler ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed 2 times with EB2 without NaF or PhosStop and one time with λpp buffer (New England Biolabs, Ipswich, MA) before resuspension in 40 μl of λpp buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 100 ng of template DNA was used in a 2 hour in vitro transcription reaction using the HiScribe T7 High Yield RNA (New England Biolabs, Cat. E2040S) kit according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... after which 68 µL RNase-free H2O was added with 10 µL DNase I Buffer and 2 µL DNase I (New England Biolabs, Ipswitch, MA). The resulting mix was incubated for 15m at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 9 DNA fragments encoding the partial genome of SARS-CoV-2 were prepared by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Cat# M0491S). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Molecular Biology 2023Quote: ... spanning the whole SARS-CoV-2 genome were amplified using the high-fidelity proofreading enzyme Q5® High-Fidelity DNA Polymerase (NEB, M0491L) in a 25 µL reaction volume using respective primers (fig ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Immunology 2023Quote: ... PCR was performed with 2 ng of library input per reaction and 7 cycles of amplification using Q5 DNA polymerase (New England Biolabs, catalog # M0491S). Libraries were quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... was amplified from JE2 using the primer pair 3810/9647 and the snap-tag coding sequence amplified from pSNAP-tag (T7)-2 (New England Biolabs, NEB; Supplementary Information) using the primer pair 9631/9632 ...
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bioRxiv - Synthetic Biology 2023Quote: BigBlocks were PCR-amplified with dedicated primer pairs in a 25.0 μL PCR reaction mix composed of 0.25 μL Q5® Hot Start High-Fidelity DNA Polymerase 2 U/μL (NEB® M0493), 5.0 μL Q5® 5X buffer (NEB® B9027) ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were selected for cDNA target fragments of 200 bp on 2% low-range Ultra agarose followed by PCR amplification using Phusion DNA polymerase (NEB, United States) for 15 PCR cycles ...
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bioRxiv - Plant Biology 2024Quote: ... mCherry-2-L and the PIF5 coding sequence into pJHA212G80 containing the rbcS terminator using Gibson Assembly (New England Biolabs, Ipswich, MA). The resulting construct was transformed into pif5-3 using Agrobacterium-mediated transformation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cat. No. B6002S]; 2.5 µl 100 µM gRNA [ordered as a synthesized oligo]; 2 µl 100 µM LbaCas12a [NEB, Cat. No. M0653T]) was incubated for 5 minutes at room temperature.
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bioRxiv - Genetics 2024Quote: ... sgRNA complementary to the central part of the hACE2 (Table 2) was synthesized with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, USA). Library was loaded onto the FLO-MIN106 flow cell and sequenced with GridION (Oxford Nanopore ...
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bioRxiv - Genomics 2024Quote: ... 0.5 µM GAT-7N primers (5′-GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/µl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L) and incubated for 11 cycles in the BioRad thermocycler ...
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bioRxiv - Molecular Biology 2024Quote: ... The NicE-view labeling mix was based on previously published protocols24–26 and consisted of 1x NEBuffer 2 (NEB, Ipswich USA, #B7002S), 30 µM dGTP (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Adapters were subsequently ligated to deacylated and demethylated RNA templates using a truncated KQ T4 RNA ligase 2 (New England Biolabs, cat# M0373L) for 1 hour at 25°C with 20% PEG8000 (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... were ligated to the 3’ ends of total RNA from EVs and cellular samples isolated from an RN cell line using a truncated KQ T4 RNA ligase 2 (New England Biolabs, cat# M0373L) for 1 h at 25°C ...
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bioRxiv - Genomics 2024Quote: ... 0.5 µM GAT-7N random hexamer primers with an adapter sequence and 2 units/µl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L). DNA was further amplified as in the pre-amplification step above ...
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bioRxiv - Systems Biology 2022Quote: ... 5’-AAAC(N)19-20-3’) by combining 1 μl of each 100 μM oligonucleotide with 1 μl of 10× T4 ligation buffer (NEB cat. no. B0202S), 6.5 μl of water ...
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bioRxiv - Microbiology 2022Quote: ... We then used 1:3 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). After the HiFi assembly ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 4 hours at 37°C and treated with Antarctic phosphatase (NEB) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Annealed oligos were mixed with 4 μL 6X loading dye (NEB, B7024S), loaded onto a lab-made 8% TBE gel (Acrylamide/Bis solution 37.5:1 ...
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bioRxiv - Genomics 2020Quote: ... the circularized DNA was incubated in 1x NEBuffer 4 reaction buffer (NEB) (50 mM potassium acetate ...
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bioRxiv - Genomics 2019Quote: ... 4) We used 2.75 µl 10X RNA Fragmentation Buffer (New England Biolabs) in a total 27.5 µl fragmentation reaction volume and later on added 2.75 µl 10x RNA Fragmentation Stop Solution ...
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bioRxiv - Systems Biology 2020Quote: ... a 4-cycle PCR was performed with OneTaq polymerase (New England Biolabs) in 200 reactions (125 μL/reaction) ...
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bioRxiv - Microbiology 2021Quote: ... The parental plasmid was digested for 4 h using Dpn1 endonuclease (NEB) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... a mix of 4 μl First strand Synthesis Reaction Buffer (NEB-kit), 0.5 μl Murine RNase Inhibitor (NEB-kit ...
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bioRxiv - Genomics 2020Quote: ... 4 μL of 20 mg/mL RNase A (New England Biolabs #T3018L) is added to the RNase positive aliquot of non-lysed TSB from 1-gram cannabis flower homogenate ...
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bioRxiv - Systems Biology 2022Quote: ... 5 mM EDTA) + 4 μl of Proteinase K (20 mg/ml, NEB) at 55°C (300 rpm continuous shaking in a thermomixer) ...
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bioRxiv - Developmental Biology 2021Quote: ... by incubating with DNA polymerase I Klenow (4 mL; New England Biolabs) for 90 min at 37C with rotation ...
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bioRxiv - Immunology 2022Quote: ... and subsequently incubated with 4 U of alkaline phosphatase (New England Biolabs) at 37°C for 30 min to hydrolyze unreacted NTPs ...
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bioRxiv - Developmental Biology 2024Quote: ... 47 ml water) and 4 µL 10mg/ml Proteinase K (P8108S, NEB). The samples were incubated for 8 hours at 55 °C followed by 10 min at 98 °C to inactivate the Proteinase K ...
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bioRxiv - Biochemistry 2024Quote: ... then subsequent addition of 4 units of proteinase K (New England Biolabs) and incubation at 55 °C for 30 min ...
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bioRxiv - Genetics 2023Quote: ... 4 µl Luna universal qPCR Master Mix (New England Biolabs, Hitchin, UK) and 1.8 µl ultrapure water ...
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bioRxiv - Biophysics 2024Quote: ... 4 mM BME) and added to an amylose resin (New England Biolabs), incubating overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 40U (4 µL) T4 polynucleotide kinase enzyme (T4 PNK; NEB, Cat #M0201S), 4 µL 10X T4 Ligase Buffer (NEB ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of RNAse inhibitor and 3 μl of Antarctic phosphatase (New England BioLabs Inc.). We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Genomics 2021Quote: ... The 3′ adapter (sequence: AGATCG-GAAGAGCACACGTCTGAACTC) was ligated using T4 RNA Ligase 1 (NEB, M0204L) and purified nascent RNA using streptavidin beads (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... In short 5’-phosphorylated and 3’-OH RNA was circularized with RNA ligase 1 (NEB) followed by denaturing PAGE purification as described above ...