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Citations for New England Biolabs :
1851 - 1900 of 4067 citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Escherichia coli strains DH5α [15] and ER2523 (New England Biolabs, Ltd., UK) were grown in Luria Bertani (LB ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, cat. # C3013). The bacterial cultures were grown at 37 °C to an optical density (OD600 ...
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bioRxiv - Biophysics 2021Quote: RecA protein from Escherichia coli was purchased from New England BioLabs (NEB), Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... coli for protein purification were derivatives of pMal-c5X (New England Biolabs), which carries an AmpR marker and uses an IPTG-inducible tac promoter to expresses recombinant proteins with a factor Xa cleavable maltose-binding protein (MBP ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were expressed in Escherichia coli T7 Express (New England Biolabs) and grown in Luria-Bertani medium ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells were used (New England Biolabs GmbH, Germany,Art. No. C2987). This strain was grown in Luria-Bertani (LB ...
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bioRxiv - Plant Biology 2019Quote: ... coli BL21 and purified by Amylose Resin (New England Biolabs, Ipswich, USA). One µg of kinase protein was incubated in kinase buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain DH10-β cells (45 μL each; C3019I, New England Biolabs) thawed on ice for 10 minutes and mixed gently by tapping ...
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bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid was transformed into Escherichia coli expression strain BL2lλDE3 (New England Biolabs) for expression ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells were incubated at 37°C (30°C for NEB stable), 200 min-1 either in 5 ml (for a high copy ...
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bioRxiv - Biochemistry 2020Quote: Proteins have been expressed in Escherichia coli strain BL21 (DE3) (NEB # C2527). In brief ...
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bioRxiv - Microbiology 2020Quote: ... coli Top10 cells and purified by using a Monarch miniprep kit (NEB). A Ty tag DNA sequence was inserted at the 3’-end of the gat1 coding sequence using a Q5 site directed mutagenesis kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... transformed into NEB 5-alpha chemically competent Escherichia coli (New England BioLabs), and submitted for Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2020Quote: Escherichia coli BL21 (DE3) pRIL and SHuffle T7 (New England Biolabs, USA) strains were routinely grown in Luria Bertani (LB) ...
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bioRxiv - Biophysics 2022Quote: ... TnsC clones were transformed into Escherichia coli BL21(DE3) competent cells (NEB), while TnsB and S15 clones were transformed into homemade T1 phage-resistant pRIPL BL21(DE3) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli poly (A) polymerase (New England Biolabs® Inc., Ipswich, MA, USA), and the reaction mixture was incubated at 370 C for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: Plasmids were transformed in BL21 (DE3) competent Escherichia coli (New England Biolabs) or Rosetta (DE3 ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli sfGFP expression cassette via Q5 site-directed mutagenesis (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Molecular Biology 2023Quote: ... coli competent cells (NEB 5-alpha, New England Biolabs, Ipswich, MA, USA) that were cultured and prepared using a GenElute HP Plasmid Midi kit (NA0200-1KT ...
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bioRxiv - Biophysics 2023Quote: ... coli σ70 (σ70) was overexpressed in T7 Express cells (New England Biolabs) as inclusion bodies from the pET-28a-σ70 overexpression plasmid39 by growing the cells at 37oC to an OD600 of ∼ 0.8 ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified product was transformed in Escherichia coli NEB5α cells (NEB#C2987) to obtain colonies ...
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bioRxiv - Genomics 2023Quote: ... All constructs were polyadenylated using Escherichia coli poly(A) polymerase (NEB, M0276S) according to manufacturer’s instructions followed by a clean up using the RNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Poly-adenylation followed using Escherichia coli Poly(A) Polymerase (New England Biolabs) for 30 minutes at 37 °C and products were further cleaned with RNA clean & concentrator 5 (Zymo Research ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells were used (New England Biolabs GmbH, Germany, Art. No. C2987) for the cloning of certain plasmids ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli cells were incubated at 37°C (30°C for NEB stable), 200 min-1 either in 5 ml (for a high copy ...
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bioRxiv - Biochemistry 2024Quote: ... coli strains Nico21 (DE3) pLysS or BL21-codon plus (New England BioLabs) were used for overexpression ...
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bioRxiv - Biochemistry 2024Quote: ... coli strain T7 Express lysY/Iq (New England Biolabs, Ipswich, MA, USA) and purified by Ni- ...
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bioRxiv - Plant Biology 2024Quote: ... then transformed into DH5α competent Escherichia coli (C2987H, New England Biolabs, UK) following standard manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: Plasmids were transformed in BL21(DE3) competent Escherichia coli (New England Biolabs) or Rosetta(DE3)pLysS E ...
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bioRxiv - Biochemistry 2024Quote: ... coli exonuclease III (Xth) was performed in NEBuffer 1 (New England Biolabs) with 1 nM enzyme ...
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bioRxiv - Immunology 2019Quote: ... 2.4 mg sequence verified vector was digested at 2 μg/μL in NEB 3.1 buffer with 1200 U Nt.BspQI for 1 h at 50 °C followed by addition of 2400 U XhoI (NEB) and incubation for 1 h at 37 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). The enzymes mentioned were purchased from New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... Hi-C libraries were treated with T4 DNA Polymerase (New England Biolabs, catalog No. M0203) to remove biotin from unligated ends ...
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bioRxiv - Immunology 2023Quote: In situ Hi-C experiments were performed as previously described2 using restriction enzyme MboI (NEB) with minor modifications ...
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bioRxiv - Genomics 2023Quote: ... pooled oligonucleotides were amplified via PCR using NEBNext 2X Hi-Fi PCR Master Mix (NEB) and primers:
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bioRxiv - Molecular Biology 2024Quote: ... Y511A mutation in pBAD-His-BrxX was introduced using Q5 site-directed mutagenesis kit (NEB), while introduction of Y511A to the pBREX AL was carried by joining 2 plasmid fragments ...
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bioRxiv - Microbiology 2022Quote: ... The D614G spike plasmid was generated by introducing the mutation into the Wuhan reference strain via Q5 site-directed mutagenesis (NEB). The T95I ...
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bioRxiv - Microbiology 2022Quote: ... Mutations were introduced into the phCMV-Spike plasmids using the mutagenic primers and the Q5 site directed mutagenesis kit (NEB). In addition ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pBBR1-E and pBBR-EV were extracted using Monarch Plasmid Miniprep kit (#T1010; NEB) and following supplier’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Read 1 and Read 2 sequencing primers (sequences listed in Table S2) were added PCR fragments by T/A ligation using NEBNext Ultra II Ligation Module (E7595, New England Biolabs, Ipswich, MA). Spacers and minimal promoter were added to sequencing primer-ligated enhancers using the primers 5BC-AG-f02 and 5BC-AG-r02 (Table S2) ...
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bioRxiv - Biochemistry 2020Quote: ... was replaced with sequence for a 3×FLAG tag by Gibson Assembly (New England Biolabs) to create payload plasmid pSAB41 ...
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bioRxiv - Immunology 2022Quote: ... periplasmic expression vectors with C-terminal HA-His6 tags using Gibson cloning (New England Biolabs). Nanobodies were produced in Escherichia coli WK6 transformed with the respective expression vectors ...
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bioRxiv - Cell Biology 2019Quote: ... Fusions to the human O6-Alkyl-DNA transferase (SNAP-tag, New England Biolabs, Beverly, MA) were expressed from plasmid pAGT-Xpress ...
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bioRxiv - Biochemistry 2024Quote: Constructs bearing a C-terminal cMyc tag were cloned by site-directed mutagenesis (NEB, M0554S) using oligonucleotides as primers bearing a 5’-overhang encoding the inserted sequence ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with SNAP-tag ligand SNAP-Cell 647-SiR (New England BioLabs; S9102S) at a final concentration of 100 nM for 15 minutes followed by 30-45 minute washout in i3Neuron Maintenance Media before imaging in i3Neuron Imaging Media ...
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bioRxiv - Systems Biology 2023Quote: ... Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (NEB #M0494) with primers that add the indexed full Illumina adaptor sequences.
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bioRxiv - Biophysics 2023Quote: ... and BG-oligonuculeotides were labeled with myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer ...