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Citations for New England Biolabs :
1801 - 1850 of 4067 citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and then transformed into 5-alpha competent Escherichia coli cells (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The complete insert region was PCR amplified using Q5 Hi Fidelity DNA polymerase (NEB) and the amplicon sequenced at high coverage using Illumina MiSeq in the Complete Amplicon Next-Generation Sequencing service from the MGH CCIB DNA Core (Fig ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were conducted using NEB Phusion Hi Fi Polymerase (New England Biolabs, Ipswich, MA). Reactions were comprised of 4 µl 5X Phusion Buffer ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were conducted using NEB Phusion Hi Fi Polymerase (New England Biolabs, Ipswich, MA). Reactions were comprised of 4 µl 5X Phusion Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C to remove of biotin from non-ligated fragment ends ...
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bioRxiv - Biochemistry 2020Quote: ... His-SNAP-EB3 proteins were then coupled to SNAP-Cell 647-SiR dye (NEB) by incubation at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The expression vector pET302/NT-His was digested with EcoR1 restriction enzyme (NEB R0101S) and run on a 1.5 % agarose gel ...
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bioRxiv - Molecular Biology 2019Quote: ... 40 µg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). PCR reactions were performed with the Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs) ...
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bioRxiv - Genetics 2019Quote: ... These were used in a PCR reaction with Q5 Hi-Fidelity polymerase (NEB, USA) in combination with the pLuc-GAL5.1 reporter construct previously described 8 as template to produce pLuc-GALΔEGR ...
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bioRxiv - Genomics 2019Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
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bioRxiv - Cancer Biology 2021Quote: ... using the NEBuilder Hi Fi DNA Assembly Master Mix (New England Biolabs, Beverly, MA). For the production of lentiviral particles ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... stained in HI buffer containing 5 μM SNAP-Surface Alexa 647 (New England Biolabs) for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... and K723G).49 His/MBP-BRAF NTs were created with standard Gibson Assembly (NEB) procedure in the pET28-MBP vector from the following primers ...
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bioRxiv - Biochemistry 2023Quote: ... The His-MBP-Cas12a plasmid was transformed into BL21(DE3) cells (New England Biolabs). A single colony was used to inoculate LB media supplemented with 50μg/ml Kanamycin for an overnight culture grown at 37°C ...
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bioRxiv - Genomics 2024Quote: ... The Chromatin Conformation Capture (Hi-C) library was constructed from enzyme DpnII (NEB, USA) digestion of cross-linked DNA and sequenced to produce 150 bp paired-end reads with an Illumina Novaseq 6000 machine following the manufacturer’s sequencing protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... and E-cadherin (24E10, Cell Signaling Technology, NEB, Frankfurt, Germany, #3195, 1:400) were used for immunohistochemistry (IHC ...
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bioRxiv - Microbiology 2020Quote: ... The Flag-tagged spike gene was ligated to the linearized vector using the T4 DNA Ligase enzyme from NEB. The resulting plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... and T478K of the Delta variant was spliced into the spike expression vector by HiFi DNA assembler cloning (NEB). The plasmid was prepared and verified as described above.
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bioRxiv - Microbiology 2024Quote: ... Spike DNA fragments were introduced into the digested pCAGGS vector using NEBuilder® HiFi DNA Assembly (New England Biolabs). The product of this assembly reaction was transformed into 5-alpha Competent E ...
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bioRxiv - Molecular Biology 2020Quote: ... The gRNAs targeting SERPINB5 and Tnf were created by site-directed mutagenesis of pLenti-Il33gRNA6-puro using primers listed in Supplementary Table S2 and the Q5® Site-Directed Mutagenesis Kit (NEB) according to manufacturer protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Each potential sgRNA off target site listed in Table S2 (off-target score provided by Benchling) was screened by High-Fidelity PCR (Q5 NEB, M0491L) with primers listed in Table S5 and PCR products were sequenced using Eurofins Genomics tube DNA sequencing services ...
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bioRxiv - Immunology 2020Quote: ... Target DNA was amplified from a cDNA template or existing plasmids using primers and PCR conditions shown in Tables S2-S4 using Q5 High-Fidelity 2X Master Mix (New England Biolabs # M0494L). Primers were ordered with 5’ base extensions that overlapped expression vectors on either side of the restriction sites ...
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bioRxiv - Genetics 2019Quote: ... as shown in figure S2) were introduced into the ARSA plasmid by using Site-Directed Mutagenesis Kit (Cat. No. E0552s, NEB, USA). The sequences of mutated cDNA vectors were confirmed using the ABI3500 sequencer (Applied Biosystems Inc. ...
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bioRxiv - Genomics 2021Quote: ... standard Gibson Cloning for S1 or S2 inserts plus PCR-amplified/DpnI-digested backbone was performed using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs) at 3:1 insert to vector ratio ...
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bioRxiv - Microbiology 2021Quote: ... and fifteen repeats of the FG-repeats were amplified from genomic DNA using primers (Table S2) with Phusion High-Fidelity PCR Kit (NEB - E0553S). The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020) ...
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bioRxiv - Synthetic Biology 2020Quote: ... was then PCRed using P5 and a barcoded version of SGR-176 (see Table S2) the Q5 Hot Start High Fidelity 2x Master Mix (NEB, M0492L) with the following settings ...
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bioRxiv - Cell Biology 2021Quote: ... ORF was amplified from MCF10A human mammary cells using specific primers (listed in Table S2) with Q5 High-Fidelity 2X Master Mix (New England BioLabs, # M0492), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed with primers described in Table S2 according to the Phusion® High-Fidelity DNA polymerase supplier recommendations (New England Biolabs). Genomic DNA sequencing was performed using the 1F_typeIII primers (Table S2 ...
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bioRxiv - Microbiology 2022Quote: ... The sequencing ladders were obtained from hlgCB or hlgB PCR products (amplified with primers listed in Supplementary Table S2) and the Vent (exo-) DNA polymerase (NEB Biolabs). All samples were fractionated on a 10% polyacrylamide −8 M urea gel in 1x TBE ...
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bioRxiv - Molecular Biology 2022Quote: The tRNA-Pro coding sequence was ordered as gBlock (listed in Table S2) and was assembled with digested YCplac33 backbone using NEBuilder HiFi DNA Assembly Cloning Kit (NEB, #E5520). The tRNA-Pro probe sequence was amplified from this plasmid using the primers in Table S2 and in vitro transcribed by using Digoxigenin-11-UTP included in DIG Northern Starter Kit (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... the full region including the HrpL box promoter was PCR-amplified using primers (Supplementary Table S2) and Q5 High-Fidelity DNA Polymerase (NEB, USA). The resulting PCR fragment was gel-purified and was blunt-end-ligated into the Eco53kI (NEB ...
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bioRxiv - Genetics 2024Quote: ... 400 nM of each of the flanking primers PS1057-NGS-F and PS1057-NGS-R (S2 File) and 0.1 units/µl of LongAmp Taq DNA polymerase (NEB Cat. # M0323L). In a second amplification step ...
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bioRxiv - Microbiology 2023Quote: ... The Lysobacter gasdermin system was previously cloned10 and the Pedobacter rhizosphaerae system was amplified from genomic DNA ordered from the DSMZ (DSM 18610, IMG gene accessions in Table S2) and cloned using the NEBuilder HiFi DNA Assembly cloning kit (NEB E5520S). As a negative control ...
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bioRxiv - Synthetic Biology 2024Quote: ... Oligodeoxynucleotides (Table S2) for detection of specific RNA species were end-labeled with [γ- 32P]-ATP using T4 polynucleotide kinase (PNK) (NEB). Prehybridization and hybridization were performed in Church buffer [0.5 M phosphate buffer (pH 7.2) ...
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bioRxiv - Bioengineering 2024Quote: ... The transgene and sex of the F0 and F1 pups were determined by 3 PCRs of the Y chromosome using oligonucleotides described in Table S2 and LongAmp DNA polymerase (New England Biolabs, USA). Thermocycling conditions were as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA from clinical samples (Table S2, S3) was PCR amplified using Taq DNA polymerase with ThermoPol®-Buffer (New England Biolabs). Briefly ...
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bioRxiv - Biophysics 2020Quote: ... the cells were first labelled with 5 µM biotin-conjugated SNAP-tag substrate (NEB) in tissue culture medium ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... SNAP-Tag constructs were labeled with SNAP-Cell® 430 (New England BioLabs, S9109S) or SNAP-Cell® 647-SiR (New England BioLabs ...
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bioRxiv - Biophysics 2022Quote: ... purified proteins were labelled with SNAP-Surface Alexa Fluor 647 tag (New England Biolabs) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... The tags were removed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) to generate wild-type ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... CUT&Tag libraries were prepared with NEBNext HiFi 2x PCR Master Mix (NEB M0541S) and indexed primers (Buenrostro et al. ...
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bioRxiv - Neuroscience 2022Quote: ... The chemical substrates were SNAP- tag ligands (SNAP surface 549 - BG 549 [NEB, S9112S]) and CLIP-tag ligands (CLIP surface 647 - BC 647 [NEB ...
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bioRxiv - Immunology 2022Quote: ... To digest remaining M1 tag forward oligo: 3 µl 20U/ µl Exonuclease I (NEB) was added and samples were incubated for 1 h at 37°C ExoI denaturation was performed for 5 min at 98°C ...
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bioRxiv - Cell Biology 2023Quote: ... To amplify the SNAP tag sequence out of the pSNAPf plasmid (New England Biolabs), the following primers were used ...
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bioRxiv - Plant Biology 2022Quote: ... coli BL21 (DE3) and purified using Amylose Resin (MBP, New England Biolabs) according to the manufacturer’s introduction as well as recombinant Arabidopsis histone MBP-H31-57 ...
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bioRxiv - Microbiology 2019Quote: ... Bacillus subtilis 3610 (33) and Escherichia coli strains NEB10β (New England Biolabs) and WM3064 were cultured aerobically on LB agar or in LB broth ...
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bioRxiv - Genetics 2019Quote: ... coli strains NEB10®-beta (New England BioLabs Inc., Ipswich, MA, USA), and XL1-blue (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express lysY/Iq (New England Biolabs, Ipswich, MA, USA) and purified by Ni- ...