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Citations for New England Biolabs :
1701 - 1750 of 4067 citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... All pCas9 constructs were transformed into Escherichia coli (NEB Turbo) by heat-shock and made electrocompetent as described (protocols.io ...
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bioRxiv - Microbiology 2020Quote: ... coli cloning strains such as NEB-5a (New England Biolabs) or BH10c,48 with antibiotics added at the following concentrations ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Stbl competent cells (New England Biolabs, Ipswich, MA, USA), colonies were screened by PCR ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5-alpha cells following manufacturer’s protocol (New England Biolabs). Sanger sequencing was used to confirm plasmid construction ...
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bioRxiv - Immunology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs #C3020K), and bottlenecked to ∼1 × 105 cfus (an average of >25 barcodes per single-mutant) ...
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bioRxiv - Biochemistry 2022Quote: ... coli were transfected using the High-Efficiency Transformation Protocol (NEB). Single colonies were selected from carbenicillin LB plates and plated into 5 mL starter cultures ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli in 5 x 25 uL cell aliquots (NEB #C3020). Each cell aliquot was recovered in 1 mL outgrowth media at 37°C for 1 hr and then transferred into 100 mL of molten LB (1% tryptone ...
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bioRxiv - Microbiology 2022Quote: Escherichia coli strain NEB Stable (New England Biolabs, Ipswich, MA), Pseudomonas aeruginosa strains PA01 and PA14 (generous gift from Dr ...
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bioRxiv - Genetics 2024Quote: ... coli competent cells (New England Biolabs, MA, USA, Cat # C2987H) from the pDONR 221 entry vector to the pGW-HA.attB destination vector using Gateway cloning and the sequences were confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Competent strains of Escherichia coli DH5-α (New England Biolabs), used for DNA cloning and plasmid propagation ...
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bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 strain resistant to phage T1 (New England Biolabs) harboring the pRARE2 plasmid and plated onto LB+Agar plates supplemented with kanamycin (Kan ...
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bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
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bioRxiv - Biophysics 2024Quote: ... and then transformed BL21(DE3) Escherichia coli (New England BioLabs) with this plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... Coli BL21(DE3) strain (purchased from New England Biolab, NEB), BL21Marioentte (purchased from Addgene ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain SHuffle T7 (New England Biolabs Inc., Massachusetts, USA) and plated on LB agar containing 30 μg/mL kanamycin and 2% glucose ...
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bioRxiv - Bioengineering 2023Quote: ... coli MG1655 gDNA (cat no. T3010, New England Biolabs Inc.) via PCR ...
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bioRxiv - Microbiology 2023Quote: ... Strains used were: Escherichia coli T7 Shuffle (New England Biolabs) to express Rv0365c ...
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bioRxiv - Plant Biology 2024Quote: ... coli strain BL21 (DE3) using Amylose Resin (New England Biolabs), as previously described 44.
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bioRxiv - Synthetic Biology 2024Quote: ... coli dam-/dcm- was purchased from New England BioLabs (NEB) and used for de-methylation of plasmid DNA ...
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bioRxiv - Biochemistry 2020Quote: ... Tag introduction and mutagenesis was achieved through Gibson assembly (New England Biolabs) to generate SMC1-His ...
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bioRxiv - Microbiology 2022Quote: ... Affinity tags were introduced by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) into the expression vectors encoding Rae1 and Nup98 using primers in Table 1 ...
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bioRxiv - Biophysics 2021Quote: ... and the His6-MBP tag was subsequently recaptured using amylose resin (NEB). The flow-through was loaded onto a Q-HP anion exchange column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently the 6xHIS tag was cleaved using the TEV Protease (NEB) by adding TEV enzyme assuming all protein isolate was 6xHIS tagged based on the activity of the TEV protease ...
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bioRxiv - Biochemistry 2023Quote: ... and SNAP tags were stained with SNAP-Cell® 505-Star (NEB). HaloTag® Ligands TMRDirect were added to medium to a final concentration of 50nM and incubated overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The 3XFLAG tag was replaced by Q5® site-directed mutagenesis (NEB) with either a Myc-tag or Strep-tag®II ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins with MBP-tag were loaded onto amylose resin (New England Biolabs) after elution from Ni-NTA ...
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bioRxiv - Immunology 2021Quote: ... The kRSV-DB1-QUAD plasmid and spike insert were digested with the enzymes AatII and SalI (NEB) and ligated with T4 DNA ligase (NEB ...
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bioRxiv - Biophysics 2021Quote: ... Complexes were prepared by incubating spike ectodomain preparations with either ACE2 (residues 18–615, New England Biolabs), VH ab8 ...
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bioRxiv - Microbiology 2022Quote: ... Spike variants with single-site residue substitutions were generated using Q5® High-fidelity DNA polymerase (NEB)-based site-directed mutagenesis.
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bioRxiv - Synthetic Biology 2022Quote: In vitro transcription/translation by codon skipping of the short FLAG tag-containing peptides X-Val-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (XV-Flag) where X = 7, 13, 14, or 15 was carried out using the PURExpress® Δ (aa, tRNA) Kit (New England Biolabs, E6840S) based on a previous protocol with slight modifications 16 ...
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bioRxiv - Synthetic Biology 2021Quote: ... To this end pCANTAB6_VhhTD4 plasmid (Supplementary Fig. S2) was used as DNA template for PCR using Q5 DNA polymerase (NEB) in three different reactions each containing the oligos 1 and 2 (for H107Y) ...
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bioRxiv - Biochemistry 2024Quote: ... appropriate fragment of SynPOR gene was amplified with specific primers with additional fragments corresponding to amplified pET15b_AtPORB (Table S2) with Q5 polymerase (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... HA-NONO mutants were PCR-cloned with primers (Supplemental Table S2) and a Q5 site-directed mutagenesis kit (NEB) using the manufacturer’s protocol and verified by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... The Rap1 arrays initially cloned in a pUC19-derived vector (Table S2) were digested with PvuII (New England Biolabs) and subsequently gel isolated ...
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bioRxiv - Molecular Biology 2023Quote: ... and S2) or EDTA (25 mM final concentration) plus 1.8-units Protease K (all other Figures, New England Biolabs), and incubating at 25°C for 15 min ...
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bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext Hi-Fi 2x PCR Master Mix (NEB (Ipswich, MA, USA) M0541S) ...
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bioRxiv - Microbiology 2020Quote: ... and ligation with T4 ligase (Bioconcept) or by Hi-Fi Gibson assembly (NEB). PCR were performed using Phusion polymerase (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: His-SpCas9-GFP was expressed in and purified from BL21 (DE3, NEB, C2527H) bacteria as previously described 47 ...
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bioRxiv - Microbiology 2024Quote: ... and joined into the linearized vector by Hi-Fi Gibson DNA Assembly (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... His- tagged proteins were purified by NEBExpress® Ni-NTA Magnetic Beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
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bioRxiv - Genomics 2019Quote: ... Then brought volume to final 1200 μl with final 1x NEB DNA ligase reaction buffer (NEB, B0202S) and 1x BSA (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... each lysed by incubation in 1000 μL RIPA buffer + 2 mM PMSF + 60 μL PIC + 112.5 Kunitz Unit/mL DNase I (RNase-free, NEB M0303) + 2.5 mM MgCl2 (Sigma 5985-OP ...
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bioRxiv - Microbiology 2022Quote: ... resulting in a linearized vector into which synthetic variant spike genes can be assembled using Gibson Assembly (NEB). The Gibson Assembly reaction was then transformed into TransforMax™ EPI300™ Electrocompetent E ...
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bioRxiv - Microbiology 2022Quote: ... PCR of a spike gene fragment was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) and the following primers ...
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bioRxiv - Biochemistry 2022Quote: For crystallization the Ioc4 PWWP domain (aa 1-178; Ioc4PWWP) was cloned into a modified pMAL-c2X vector (New England Biolabs, Liu et al., 2001) in order to produce a fusion protein with an N-terminal maltose-binding protein (MBP) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells expressing the SNAP-tag and stained with SNAP-cell 647-SiR (NEB) were excited with a white light laser at an excitation wavelength of 633 nm ...
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bioRxiv - Cell Biology 2019Quote: ... the sequence encoding SNAP-tag was PCR amplified from pSNAPf (New England BioLabs) with primers to append flanking BamHI and NotI restriction sites (Forward ...
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bioRxiv - Plant Biology 2024Quote: ... 0.4 μL Buffer Tag DNA polymerase (5 U/μL, NEB, Cat. No. B9004S), 1.6 μL dNTP (TAKARA ...