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Citations for New England Biolabs :
1701 - 1750 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II kit (NEB E7645) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) was used for in vitro transcription according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Using NEBNext Ultra II kits for Illumina (New England BioLabs, Ipswich, MA), each pool was indexed with a unique NEBNext i7 adapter and an i5 adapter containing a degenerate barcode (for removal of PCR duplicates ...
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bioRxiv - Microbiology 2024Quote: ... they were purified using Monarch DNA Gel Extraction kit (New England Biolabs) and assembled by two-step PCR (supplementary methods ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs E5520S). The fusion construct was then amplified by PCR and inserted into the multiple-cloning site of the lentiviral vector pLEX using the same NEBuilder cloning kit to generate pLEX-mNFL-GFP ...
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bioRxiv - Genomics 2024Quote: ... The DNA was extracted with NEB Monarch gel purification kit (NEB, T1020S) using two columns and eluted in 30 µL of the elution buffer.
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: DNA extraction was performed using the Monarch HWW DNA extraction kit (NEB) following the manufacturers protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) was used for generating the RNA-Seq library for each sample ...
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bioRxiv - Neuroscience 2023Quote: ... We used a magnetic mRNA isolation kit (New England BioLabs, Ipswich, MA) to enrich samples for mRNA before proceeding to library preparation ...
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bioRxiv - Synthetic Biology 2023Quote: ... cDNA was synthesized using a LunaScript® RT SuperMix Kit (NEB E3010), and real time PCR was run on a Bio-Rad CFX96 Touch Real-Time PCR Detection System using an iTaq Universal SYBR Green Supermix (BioRad #1725121) ...
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bioRxiv - Molecular Biology 2023Quote: ... Inserts were acquired by PCR using KOD Hotstart Mastermix from NovaGen and purified from gel using Monarch DNA Gel Extraction kit from NEB. All cloning was performed in E ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was purified with the NEB mRNA isolation kit (New England Biolabs). Extracted RNA was treated with DNase I (Thermo Fisher ...
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bioRxiv - Systems Biology 2023Quote: sgRNAs were transcribed in vitro using the EnGen sgRNA Synthesis kit (NEB) with oligonucleotides containing the protospacers ...
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bioRxiv - Molecular Biology 2023Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Genetics 2023Quote: ... mRNAs were purified using the Monarch RNA Cleanup kit (500 µg) (NEB) and eluted in 1X nuclease-free IDTE buffer (10 mM Tris ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used the NEBuilder kit (New England Biolabs [NEB], USA, product #E2621). Specifically ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used the NEBuilder kit (New England Biolabs [NEB], USA, product #E2621). Specifically ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using NEBNext small RNA sequencing kit (New England Biolabs) or NextFlex small RNA sequencing kit (Perkin Elmer ...
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bioRxiv - Physiology 2023Quote: ... NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes was used to generate sequencing libraries ...
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bioRxiv - Plant Biology 2023Quote: ... After purification with Monarch RNA Cleanup Kit (New England Biolabs, Ipswich, MA), RNA was reannealed ...
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bioRxiv - Zoology 2024Quote: ... The Monarch® RNA purification kit (New England Biolabs, Whitby, ON, Canada) was used to purify synthesized dsRNA following the manufacturer’s recommendation ...
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized using a cDNA Reverse Transcriptase Kit (New England Biolabs). TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR kit (New England Biolabs) with the following primers:
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bioRxiv - Microbiology 2024Quote: ... The reaction system was prepared according to the kit’s requirements (Yugong Biolabs) and fluorescence quantitative analysis was performed using the ABI 7500 PCR system (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs). Gel fragments were extracted using GeneJet Gel extraction kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: dsRNA was generated by in vitro transcription (NEB T7 E2050s transcription kit) from PCR products generated by the following forward and reverse primers (separated by comma):
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bioRxiv - Biochemistry 2023Quote: ... PRIMPOL S255D mutant was generated using Q5 site-directed mutagenesis kit (NEB). The pCS2 NLS-mCherry-PRIMPOL WT or S255D vectors were used for transfection in U2OS PRIMPOL KO cells using TransIT (Mirus ...
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bioRxiv - Biochemistry 2024Quote: ... Point mutations were generated using the Q5 Site-Directed Mutagenesis Kit (NEB). Primer sequences and plasmid construct information is listed in Supplementary Table 1.
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated from using the Monarch Total RNA miniprep kit (NEB), using the Tough-to-lyse sample protocol with bead-beating (Tissuelyser LT ...
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bioRxiv - Microbiology 2023Quote: ... The NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) PCR protocol was used ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were purified using the Monarch PCR Purification Kit (NEB # T1030L) and eluted in 10µl elution buffer.
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bioRxiv - Microbiology 2023Quote: ... cDNA reactions were purified using the Monarch PCR Purification Kit (NEB #T1030L) and eluted in 10µl elution buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were synthesized and multiplexed with NEBNext Ultra II kit (NEB). Ampure XP (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext Ultra II Directional RNA Illumina Library Prep Kit (NEB E7760) with NEBNext rRNA Depletion Kit V2 (NEB E7400) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting RNA was purified using the Monarch RNA Cleanup Kit (NEB) and stored at −80 °C.
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA was synthesized using Proto Script II reverse transcription kit (NEB) and real-time PCR reactions were performed using Quant Studio 3 (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Cancer Biology 2023Quote: ... were created using the Q5® Site-Directed Mutagenesis Kit (NEB E0554S) based on the established functional scramblase activity data [60 ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed using Monarch® Total RNA Miniprep kit (NEB) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... were prepared for sequencing with an Ultra II Library Prep Kit (NEB) and sequenced on the Illumina NextSeq platform using a 75-cycle paired end kit (NextSeq 500/550 High Output Kit).
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bioRxiv - Genomics 2023Quote: ... NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... products were purified with the Monarch PCR DNA cleanup kit (NEB, Inc.) and analyzed by endpoint PCR ...
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bioRxiv - Microbiology 2023Quote: ... purification (Monarch PCR & DNA Cleanup Kit, New England Biolabs Cat. No. T1030), and ligation with T4 DNA ligase (New England Biolabs Cat ...
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bioRxiv - Microbiology 2023Quote: ... The enzyme was removed with the Monarch PCR & DNA Cleanup Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... K68A were generated using the Q5 site-directed mutagenesis kit (NEB, E0554). The M4 mutant variant constructs were transformed and expressed in E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... mRNA was synthesized using HiScribe SP6 RNA synthesis kit (New England Biolabs) according to the manufacture’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The synthesized sgRNAs were purified using a Monarch RNA purification kit (NEB), and quality and quantity were assessed using UV-Vis spectroscopy on a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Immunology 2024Quote: ... PCR and Gibson Assembly® Cloning Kit (New England Biolabs, Ipswich, MA). The product was transformed in E ...
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bioRxiv - Microbiology 2022Quote: ... RNA extraction was performed with the Monarch Total RNA Miniprep Kit (NEB). DNAse treatment was performed as described above and cDNA was synthesized using LunaScript RT Supermix Kit (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... and cloned using the NEBuilder HiFI DNA Assembly cloning kit (NEB E5520S) into the pSG1 vector (amplified with primers OSM315 and OSM316 (Table S6)).