Labshake search
Citations for New England Biolabs :
1851 - 1900 of 3826 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Then the piggy-bac vector pPBhCMV1-miR(BsgI)-pA-3 was digested with BsgI (#R05559S, NEB) and the digested vector excised from a DNA agarose gel and the DNA purified ...
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bioRxiv - Microbiology 2019Quote: ... for each sample 3 μg of DNase-digested RNA was treated with T4 Polynucleotide Kinase (NEB) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... the 3’ ends of RNA fragments were dephosphorylated with T4 polynucleotide kinase (New England BioLabs #M0201S). A preadenylated DNA adaptor sequence was ligated to the 3’-hydroxyl ends of the RNA fragments using T4 RNA Ligase (T4 RNA Ligase 2 ...
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bioRxiv - Genomics 2021Quote: ... dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1 ...
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bioRxiv - Genomics 2021Quote: dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1 ...
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bioRxiv - Genomics 2021Quote: ... followed by an overnight incubation at 16°C with 3 µL T4 DNA ligase (NEB, M0202). Samples were purified with phenol-chloroform and used as 3C templates for Taqman-qPCR ...
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bioRxiv - Biochemistry 2021Quote: ... Concentrated medium was collected from the filters and 3 μl of PNGase F (New England Biolabs) was added to each sample then incubated at 37 °C for 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with media containing 3 µM SNAP-Cell block (New England BioLabs cat. # S9106S) and cells maintained at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Table 3) region of clpP1 was amplified by PCR (GC buffer, Phusion polymerase, New England Biolabs) using isolated L ...
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bioRxiv - Microbiology 2021Quote: ... 5’ triphosphorylated RNA was capped with 3’-desthiobiotin-TEG-guanosine 5’ triphosphate (DTBGTP) (New England Biolabs) using the vaccinia capping enzyme (VCE ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA fragments were end-repaired and A-Tailed using Klenow fragment (3’-5-exo-) (NEB, M0212L), the DNA fragments were ligated to illumina adaptors (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... 3′ dA-tailed using NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB E7546L) and were purified with paramagnetic beads ...
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bioRxiv - Genetics 2021Quote: ... Singlet and duplet pools were A-tailed using using Klenow HC 3’→5’ exo- (#M0212L; NEB).
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bioRxiv - Cancer Biology 2020Quote: ... Positive control slides were treated with 3 U/mL DNase-1 (New England Biolabs, Cat. M0303) for 10 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... and a NEBNext Multiplex Oligos for Illumina (Index Primers Set 3) (Code E7710; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and ligated to the 3′ adapter by incubating with T4 RNA ligase 2 truncated mutant (NEB) and 1 µg of pre-adenylated adapter (5′rAppCTGTAGGCACCATCAAT/3ddc ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A deoxyadenosine triphosphate was added at each 3’end with the Klenow fragment (New England Biolabs), and (3 ...
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bioRxiv - Neuroscience 2023Quote: ... 3 μg of total RNA was reverse transcribed using LunaScript cDNA Synthesis Kit (New England Biolabs). Gene-expression levels were quantified by real-time quantitative PCR (iCycler iQ BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... The tRNA 2’-3’ cyclic phosphate was removed by treatment with T4 PNK (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A deoxyadenosine triphosphate was added at each 3’end with the Klenow fragment (New England Biolabs). Illumina Truseq adapters (Affymetrix ...
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bioRxiv - Genetics 2023Quote: ... then dATP was added to the 3′ ends of the DNA using Klenow exo-(NEB #M0212). The DNA was then subjected to double-sided SPRI bead size selection (AMPure XP beads ...
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bioRxiv - Microbiology 2023Quote: ... Pre-adenylated L3-1R-App 3’ adaptors were ligated using T4 RNA ligase 1 (M0204, NEB) for 75 min at 25°C ...
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bioRxiv - Biophysics 2022Quote: ... The gene sequences were flanked by restriction enzyme sites for 5’ NdeI and 3’ EcoRI (NEB). Genes were cloned into pET21a using standard protocols from NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ and 3’ homology arms and the insert were cloned into the pUC19 vector (NEB, #N3041S) by using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer IIIb (3% Triton X-100, 1x T4 ligase buffer, 1x protease inhibitors; NEB, Sigma-Aldrich) was added to a final volume of 1 ml and sample was incubated for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... newly deposited CENP-A was labelled with 3 µM SNAP-Cell® 647-siR (S910102S, NEB) for 30 mins before washing excess with media and allowing to grow for a further 30 mins ...
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bioRxiv - Molecular Biology 2024Quote: ... About 3 µg of DNA from each sample was digested with two restriction enzymes (PstI; NEB catalog #R0140 and XhoI ...
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bioRxiv - Bioengineering 2024Quote: ... The extracted DNA (3 μg) was fully digested with NcoI restriction enzyme (New England Biolabs, USA), separated in a 0.8% agarose gel and blotted onto a Hybond+ Nylon membrane (Amersham Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... we combined 10 μg of λ-DNA (NEB, N3011S) and 5 μl of a 10X polymerase buffer (50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 μl RNase H (New England Biolabs M0297S). The nuclei were prepared for ChIP as described above.
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bioRxiv - Genomics 2020Quote: ... and 1 μl (10 U/μl) of APE1 (NEB) in a final volume of 40 μl at 37°C for 20 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10-Beta electrocompetent (New England Biolabs, MA, USA) or in-lab made equivalent for increased transformation efficiency ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 1 µl of BsaI 10 U/µl (NEB) were added to 7 µl of a mixture of the DNA parts used for each TU ...
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bioRxiv - Biochemistry 2020Quote: ... the plasmid (10 μg) was completely digested by NEB BsmBI or EarI restriction enzymes (Supp ...
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bioRxiv - Genetics 2021Quote: ... 10 μl of 5X OneTaq buffer (New England BioLabs), 1 μl of 10 mM dNTPs and 0.5 μl of OneTaq Polymerase (New England BioLabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 µl murine RNase inhibitor (200U, NEB M0314L) and incubated for 2 h at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... gDNA (10 μg) was digested using XbaI (NEB R0145) and run out on a 1% agarose gel ...
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bioRxiv - Biochemistry 2021Quote: ... and T4 PNK (10 U; M0437M, NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 10 units of T4 RNA Ligase 1 (NEB). Ligation reactions were incubated at 25 °C for 16 hours ...
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bioRxiv - Systems Biology 2020Quote: ... 0.4 μL 10 mM dNTP Solution (New England Biolabs), 4 μL 5x Phusion HF Buffer (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Broad Range (10-250 kDa) (New England Biolabs P7719S) was used as a protein standard ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 μL of 10 mM dNTPs (New England BioLabs), and RNase-free water (up to 6.5 μl ...
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bioRxiv - Microbiology 2022Quote: ... Following incubation with 10 U DpnI (New England Biolabs) to eliminate the remaining vector DNA ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 μl of 10 mM dNTPs Mix (NEB, N0447S), either 4.0 μl of primer set 1 or 3.98 μl of primer set 2 ...
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bioRxiv - Bioengineering 2022Quote: ... 10 nM m13mp18 ssDNA scaffold (Bayou Biolabs, Metairie, LA) was folded with 75 nM staples (IDT ...
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bioRxiv - Cell Biology 2022Quote: ... T4 Polynucleotide Kinase (1:10, #MO201S, New England Biolabs), T4 Ligase reaction buffer (#B0202S ...
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bioRxiv - Microbiology 2021Quote: ... coli DH10B (New England Biolabs 10-beta, cat#C3020K), selected on LB supplemented with 50 μg/ml kanamycin (LB+KA50) ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μl of 10 mM dNTPs (New England Biolabs), 2.5 μl of 10 μM oligonucleotide A (unmodified forward primer ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μl of 10 mM dNTPs (New England Biolabs), 10 μl of Sulfolobus DNA polymerase IV (New England Biolabs ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 10 μL of Q5 2X Master Mix (NEB) + SYBR Green ...