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Citations for New England Biolabs :
1651 - 1700 of 3826 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... We used 0.6 µl of NEBNext Second Strand Synthesis Enzyme Mix with 1x NEBNext Second Strand Synthesis Reaction Buffer (New England Biolabs) in a total volume of 12 µl for the 2nd strand cDNA synthesis ...
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bioRxiv - Synthetic Biology 2020Quote: ... All digested products were column purified and mixed at a 6:1 molar ratio in the presence of T4 DNA ligase (NEB).
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bioRxiv - Microbiology 2021Quote: ... primers oJV5-6 were used to remove ackA from pJV204 using the Q5 site-directed mutagenesis kit (NEB CN# E0554S) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Wild-type MukB was 6×His-tagged at the C-terminus and was expressed from plasmid Pet21 in C3013I cells (NEB). For Immobilized His6-tagged MukB ...
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bioRxiv - Genomics 2019Quote: ... The first 60 μL PCR reaction (also run as three parallel 20 μL reactions) included 6 μL of 10X ThermoPol Taq buffer (NEB), 0.96 μL of 5 μM forward primer (0.08 μM final) ...
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bioRxiv - Biochemistry 2021Quote: ... 150 μl of the remaining sample was mixed with 6 μl (1 μl per 25 μl sample volume) of Thermolabile Proteinase K (NEB), incubated at 37 °C for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... were PCR-amplified using primers that contained restriction sites for EcoRI and SacI (Table S5, primers No. 1-6) and the high-fidelity polymerase Q5 (New England Biolabs) according to vendor’s manual ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA was synthesized from 1 μg of total RNA using M-MuLV Reverse Transcriptase and Random Primers 6 (both New England Biolabs) at 42 °C for 60 min and diluted in 1:4 ratio by PCR grade water ...
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bioRxiv - Plant Biology 2020Quote: ... for sgRNA-LecRK-I.1 and pMR218 (L5-L2) for sgRNA-LecRK-I.6 via a cut-ligation reaction with BbsI (New England Biolabs) and T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 h at 37°C in the absence or presence of RNase H or RNase A (40 U, NEB). Digested nucleic acids were cleaned with phenol-chloroform extraction and resuspended in nuclease-free water ...
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bioRxiv - Synthetic Biology 2019Quote: ... Approximately 200−250 pmol of RNA was mixed with 400 pmol of reverse primer and 6 μL of dNTP mix (25 mM each, New England Biolabs) in nuclease-free water at a final volume of 35 μL ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Genomics 2019Quote: ... the immunoprecipitated protein-bound sepharose beads was washed four times with PBS and suspended in the DNaseI digestion buffer containing DNaseI (6 Units of M0303S NEB). The samples were incubated for 10 minutes at room temperature and centrifuged at low speed to separate two fractions ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa-CENP-A-SNAP cells were grown in 6 well plates and pulse labeled with tetra-methyl-rhodamine-conjugated SNAP substrate (TMR-Star; New England Biolabs) at 4µM final concentration ...
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bioRxiv - Developmental Biology 2020Quote: ... and microRNAs were isolated by size selection on 6% TBE PAGE gels 1mM (Novex) with clean up performed using Monarch PCR and DNA clean up kit (NEB), according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The 5’ and 3’ arms (5 to 8 kb) were amplified from a C57Bl/6 BAC clone by PCR using Q5 polymerase (New England Biolabs) and inserted into a cloning vector that contains frt-flanked SV-Neo for positive selection and Pgk-DTA and HSV-TK genes for negative selection (Jarvie et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The products were purified using the Zymo Research kit and eluted with 6 uL water and 1 uL was electroporated into DH10B cells (NEB). Electroporated cells were recovered in 975 uL of LB and plated on LB agar containing carbenicillin (100 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: mRNA was isolated from 6-well plates showing a cell confluency of ∼90% using the Monarch Total RNA Miniprep kit (NEB). siRNA transfection was performed 48 hours prior to RNA isolation using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... The DNA with end tags was oxidated by adding 15 μL TET2 reaction mix (6 μL TET2 reaction buffer plus reconstituted TET2 reaction buffer supplement (NEB), 0.6 μL oxidation supplement (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 μl of ATP (New England Biolabs) at 30 °C for 45 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 10 U/uL of 5’ deadenylase (NEB M0331S), 10 U/uL Rec J exonuclease (Epicentre RJ411250) ...
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bioRxiv - Genomics 2022Quote: ... 10 μl NEBNext Quick T4 DNA Ligase (NEB) and 5 μl Adapter Mix H (ONT) ...
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bioRxiv - Genomics 2019Quote: ... 10 μl NEB Buffer 2 (New England Biolabs), 12 μl dNTP mix (2.5 mM) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.25 µL BsaI at 10 U/µL (NEB). Cycling conditions were ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 10 U of DNAse I (New England Biolabs), and 1 μL of 100 mg/mL RNAse A (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... 10 µL of 2% BSA (New England Biolabs), 930 µL of nuclease-free water and supplemented with 0.2 U/ul RNaseIN Plus (Promega ...
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bioRxiv - Genomics 2021Quote: ... 10 or 128 units of MNase (NEB, # M0247S) was added and after incubation (10 min ...
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bioRxiv - Genomics 2021Quote: ... BsaI (10 U, New England Biolabs Japan, Tokyo) and T4 DNA ligase (200 U ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli NEB 10-beta (New England Biolabs) was used for plasmid preparation and grown in medium supplemented as instructed by the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... and 10 units of T4 PNK (NEB, B0202S) at 20°C for 30 min ...
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bioRxiv - Genomics 2019Quote: ... 10 ul of DpnII (NEB, 50 U/µL) was added to each sample tube only ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μl of 10 mM dNTP mix (NEB), 2.5 μl of 100 mM DTT (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μl of 10 mM dNTP mix (NEB), 2.5 μl of 100 mM DTT (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 U T4 RNA ligase 1 (NEB) and 1 μM of 5’ adaptor oligo (total reaction volume = 20 μl) ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μl shrimp alkaline phosphatase (New England Biolabs), 2 μl RNaseOUT (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... 0.4μL 10 mM dNTP Solution (New England Biolabs), 4μL 5x Phusion HF Buffer (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid was transformed into 10-beta cells (NEB) and clone sequence verified by sanger sequencing.
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bioRxiv - Systems Biology 2020Quote: ... 10 μL Gibson assembly master mix (NEB E2611) and nuclease free water to a final volume of 20 μL then incubated the reaction at 50°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl 10× T4 RNA ligase buffer (NEB), 20 U T4 RNA ligase enzyme ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl of 10× Antarctic Phosphatase buffer (NEB), 1 U Antarctic Phosphatase ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10 nmol ATP (New England Biolabs, # P0756S) were added to the 17.5 μl annealing products ...
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bioRxiv - Genomics 2022Quote: ... and 10 µl Rnase inhibitor (NEB cat# M0314B) and sonicated for 5 min with 30 second on/off cycles ...
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bioRxiv - Genomics 2022Quote: ... 10 μL of Exonuclease I (New England Biolabs), and 170 μL of nuclease-free water (Nacalai Tesque ...
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bioRxiv - Bioengineering 2022Quote: ... composed of 1 μL 10× buffer 3.1 (NEB), 200 ng Cas9 RNP ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 units of T4 polynucleotide kinase (NEB, M0201S) and 1,200 units of Rapid T4 DNA ligase (Enzymatics ...
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bioRxiv - Molecular Biology 2020Quote: ... 15ul of 10 mg/mL BSA (NEB, B9000), 10ul of 400 U/mL T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2022Quote: ... 10 µL of Q5 GC Enhancer Buffer (NEB), 2.5 µL of a forward primer (10µM) ...
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bioRxiv - Microbiology 2022Quote: ... 0.6 μl T5 exonuclease (10 U/μl, NEB), 20 μl Phusion DNA polymerase (2 U/μl ...
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bioRxiv - Biochemistry 2022Quote: ... coli NEB 10-beta cells (New England Biolabs) and plated on selective LB agar plates (50 µg/mL kanamycin ...
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bioRxiv - Cell Biology 2022Quote: ... 10 units T4 polynucleotide kinase (New England Biolabs), 1X T4 Polynucleotide Kinase Buffer (New England Biolabs ...