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Citations for New England Biolabs :
1801 - 1850 of 6947 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... and IFIH1 were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The one-step cDNA synthesis was carried out using LunaScript™ RT SuperMix Kit (NEB Biolabs, USA) followed by qRT-PCR master mix preparation using Luna® Universal qPCR Master Mix (NEB Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... The one-step cDNA synthesis was carried out using LunaScript™ RT SuperMix Kit (NEB Biolabs, USA) followed by qRT-PCR master mix preparation using Luna® Universal qPCR Master Mix (NEB Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: Samples with CT <25 were submitted to cDNA synthesis protocol using LunaScript™ RT SuperMix Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Comparable total RNA quantities were used for reverse transcription with LunaScript RT SuperMix Kit (New England Biolabs). cDNA was amplified with Phusion polymerase for 30-36 cycles and resulting PCR products were separated on a standard 1% agarose gel next to a 100 bp ladder (NEB) ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized for qPCR quantification using the LunaScript RT SuperMix Kit (New England Biolabs; Massachusetts, USA). qPCR reactions were composed of 7.5 μL Power SYBR Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNAse treatment was performed as described above and cDNA was synthesized using LunaScript RT Supermix Kit (NEB). RT and non-RT vials were checked by PCR with primers 7,8 for PgV (Supplementary File 2) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA were synthesised from 1 µg RNA by reverse transcription using LunaScript RT SuperMix Kit (NEB; E3010). qRT-PCR was performed using Luna Universal qPCR Master Mix (NEB ...
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bioRxiv - Microbiology 2022Quote: ... the RNA was reverse transcribed using the LunaScript RT Supermix Kit (New England BioLabs, NEB; Ipswich, MA). Tiled 1200 bp amplicons were generated using midnight primers (IDT ...
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bioRxiv - Microbiology 2022Quote: ... the RNA was reverse transcribed using the LunaScript RT Supermix Kit (New England BioLabs, NEB; Ipswich, MA). Tiled 1200 bp amplicons were generated using midnight primers (IDT ...
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bioRxiv - Neuroscience 2023Quote: ... 400ng of total RNA was used for reverse transcription using LunaScript™ RT SuperMix Kit (NEB, USA). qPCR was performed using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription for first-strand cDNA synthesis was performed by using the LunaScript RT SuperMix Kit (NEB) containing both oligo(dT ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription reactions (RT +) were then performed with 5 µg of RNA and random hexamers (NEB, #S1330S), using the Superscript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Each reverse transcription reaction contained 8 μL template RNA and 2 μL LunaScript RT SuperMix (NEB #E3010). The reaction condition for reverse transcription was ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 µg of total RNA using the LunaScript RT SuperMix kit (NEB, UK) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: We performed RT-qPCR on select genes using the Luna Universal One-Step Kit (New England Biolabs) and QuantStudio 5 (Thermo) ...
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bioRxiv - Microbiology 2023Quote: cDNA was synthesized from extracted viral RNA using the LunaScript® RT SuperMix Kit (NEB, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of total RNA was converted into cDNA with LunaScript RT SuperMix kit (New England Biolabs). The cDNAs were used as templates for real-time PCR and ran on StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was reverse transcribed from 1 µg of total RNA using LunaScript RT Supermix kit (NEB #E3010) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... First strand cDNA synthesis was performed with LunaScript RT SuperMix Kit (E3010L; New England Biolabs, Ipswich, MA), and 100 ng-1 μg total RNA was used for one 20 μl RT reaction ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription reactions were performed using a LunaScript RT SuperMix Kit (#E3010L, New England Biolabs, Ipswich, MA) with 500 ng of total RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The regular PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs). The PCR products that targeted exon18-10 and exon9-10 were then combined for each sample and run on 2% agarose gel ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reactions (total 30 μL) included 15 μL PhusionR High-Fidelity PCR Master Mix (New England Biolabs), 0.2 mM primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were pooled and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Sequenced PCR products showing overlapping peaks in their chromatograms were cloned using NEB PCR cloning kit (NEB #E1202S) and then sequenced to define the identity of mutations carried by F1 heterozygous animals ...
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bioRxiv - Microbiology 2019Quote: ... the PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg; New England Biolabs GmbH). For the second PCR ...
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bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
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bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was performed in 1X Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB M0531) and PCR cycling at 98°C for 30s ...
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bioRxiv - Plant Biology 2021Quote: ... transposed DNA was PCR amplified with 12 cycles using Next High-Fidelity 2×PCR Master Mix (NEB, M0541) with Nextera DNA CD Index primers ...
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bioRxiv - Biophysics 2022Quote: ... PCR cleanup was carried out on each amplicon sample using the Monarch PCR cleanup kit (New England Biolabs). The amplicons were then dual-indexed for paired-end read Illumina sequencing using Nextera primers N701 and N702 for the PEL and SUP of the 5.5 h time point and N705 and N706 for the PEL and SUP of the 9.0 h time point ...
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bioRxiv - Immunology 2022Quote: ... Final amplification PCR was performed as described before by adding 8 µl of 2 x PCR MM (NEB) with number of cycles according to qPCR (usually Cq values were around 20) ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription and qRT-PCR analysis was performed by Luna universal one- step qRT-PCR kit (NEB # E3005L) with primers list in Table 9.
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bioRxiv - Neuroscience 2020Quote: ... PCR amplification was performed for 15 cycli using the NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541), followed by a 0.8x beads clean-up ...
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bioRxiv - Microbiology 2019Quote: ... All the PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs). The libraries generated with TruSeq DNA PCR-Free Sample Preparation Kit were sequenced using paired-end Illumina sequencing (2 × 250 bp ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR reactions were performed with the Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs). All inserts were verified by sequencing (GENEWIZ) ...
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bioRxiv - Genomics 2020Quote: ... The final PCR amplification was performed using 2X Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) and final 0.1 μM of PE1.0 and corresponding multiplex PE2.0_MTX (Table S3) ...
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bioRxiv - Molecular Biology 2021Quote: ... Tandem PCR was performed by Q5 PCR (0.5 μl Q5® HiFi DNA Polymerase (New England Biolabs, M0491), 1x Q5 reaction buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 11) with 15 cycles of PCR using NEBNext® Q5® Hot Start HiFi PCR Master Mix (NEB), which allowed to add Gap Repair recombination sequences for the cloning in Gal4-AD prey plasmid pP7 ...
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bioRxiv - Immunology 2019Quote: ... the PCR product was subjected PCR using Q5® Hot Start High-Fidelity 2X Master Mix (NEB; M0494S) in two separate reactions to amplify the KK10 or the KY9 epitope ...
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bioRxiv - Immunology 2020Quote: ... and amplified by PCR for 11 cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). PCR products were purified using a PCR Purification Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Inverse PCR was performed using NEBNext high-fidelity 2X PCR master mix (New England Biolabs, catalog no. M0541). DNA was purified with SPRISelect beads ...
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bioRxiv - Genomics 2021Quote: ... we performed an initial PCR for locus-specific amplicon enrichment using NEBNext 2X High Fidelity PCR MM (NEB) and 5’-biotinylated stub adapter primers specific to appropriate genomic regions to be interrogated (Figure 5-table supplement 1) ...
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bioRxiv - Immunology 2022Quote: ... and amplified by PCR for 11 cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). PCR products were purified using a PCR Purification Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplification of the dhodh locus was performed using Phusion High-Fidelity PCR Master Mix (New England BioLabs) as per the protocol ...
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bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
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bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
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bioRxiv - Molecular Biology 2022Quote: ... tagmented DNA was PCR amplified using 1x Phusion® High-Fidelity PCR Master Mix with GC Buffer (NEB) and 1.25 μM i5 and i7 PCR primers (Nextera® Index Kit (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were undertaken using sporocarp extractions using the PHusion High-Fidelity PCR Master Mix (New England Biolabs). Libraries were generated using the TruSeq DNA PCR-Free Prep Kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: The amplicons from the Q5 PCR step were cleaned up using Monarch® PCR & DNA Cleanup Kit (NEB). The purity and size of the DNA bands were visualized by running a 1% agarose gel ...