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Citations for New England Biolabs :
1751 - 1800 of 6947 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Phusion® High-Fidelity PCR Master Mix was used to prepare the PCR library (New England Biolabs). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... Random mutagenesis was performed using Error-Prone PCR (EP-PCR) with Taq DNA polymerase (New England Biolabs). PCR products and restriction digestion products were purified using a GeneJET gel extraction kit (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1×loading buffer (contained SYBR green ...
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bioRxiv - Genetics 2020Quote: ... DNA libraries were then prepared by PCR amplification using NEBNext High-Fidelity PCR kit (New England Biolabs) in the presence of barcoded PCR primers (sequences provided in Ref ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion®High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1X loading buffer (contained SYB green ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products with the expected size were column-purified with the Monarch PCR & DNA Cleanup Kit (NEB) and Sanger sequenced by GATC-Eurofins or Microsynth ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was performed using the Phusion High-Fidelity PCR Kit (New England Biolabs Inc, Ipswich, UK) and the final reaction contained 250 ng of template DNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs), and the mixture PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... the scFv containing region from isolated plasmids was amplified by PCR using Q5 high fidelity PCR (NEB). Illumina NGS samples were prepped and run using the Illumina MiSeq v3 reagent kit following manufacturer’s protocols pooling four library samples per lane ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was done with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Genomics 2021Quote: ... first-level PCR reactions were performed using 1μl PCR-compatible lysate as a template for a 6.25μl Q5 (NEB) PCR reaction according to the manufacturer’s protocol (annealing temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Eluted DNA was enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was cleaned using the Monarch® PCR clean up kit (New England Biolabs T1030S) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitive PCR and limited-cycle PCR was performed using NEBNext Ultra II master mix (New England Biolabs), SYBR™ green and 0.5 µM of sample-specific P5/P7 barcoded primer mix (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L). In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragments of interest were treated with the same enzymes and cleaned by PCR cleanup (NEB). Linear plasmid pJAM2678 and each PCR product were ligated together in a reaction using T4 DNA ligase overnight at 16 °C to obtain plasmid pAKS202 that contained 150 bp upstream of the translation start site of hvo_B0047 cloned in front of bgaH ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs) and purified using Agencourt AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Synthesized testis cDNAs were used for endpoint PCR or quantitative PCR using OneTaq 2X Master Mix (NEB) or with iTaq Universal SYBR Green Supermix (BIO-RAD) ...
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bioRxiv - Immunology 2022Quote: ... Preamp PCR: Beads were resuspended in 200 µl PCR mix (20 µl 5x phusion HF buffer (NEB); 1 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... and PCRs were performed via Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB #M0531S). After PCR clean-up (Promega #A9281 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were generated using the OneTaq Quick-Load 2x PCR Master Mix with Standard Buffer (NEB) in accordance with manufacturer instructions with C ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) or RedTaq DNA Polymerase 2X Master Mix (VWR Life Science ...
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bioRxiv - Immunology 2023Quote: ... 17 PCR cycles were performed using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532) with primers and template oligo each at a concentration of 10uM ...
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bioRxiv - Genetics 2023Quote: ... The purified DNA was then amplified by PCR using NEBNext HiFi 2x PCR Master Mix (NEB M0541S), and the resulting library was purified using 1.3X SPRI purification (Omega Bio-Tek ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were amplified by PCR with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs #174M0541S) for 9 cycles in 96-well Thermal cycler (Biorad ...
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Briefly 60 ng ±10% of total RNA was subjected to small RNA library preparation by using the NEBNext Multiplex Small RNA Library Prep Set 1 and 2 for Illumina (NEB, Ipswich, MA, USA) kit according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Small RNA-seq libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, United States). Small RNAs were enriched using bead-based size selection ...
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bioRxiv - Immunology 2019Quote: ... Pooled DNA (100 ng) was fragmented and barcoded using the NEBNext→ Fast DNA Fragmentation and Library Prep Set for Ion Torrent™ (NEB Inc, UK) and IonXpress Barcode Adapters kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... PCR reaction mix was set up in duplicate for each sample with Q5 Hot Start High-Fidelity 2x Master Mix (New England Biolabs, Ipswich, MA, USA) with annealing conditions of 54°C for 25 cycles ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA libraries were constructed for sequencing on Illumina platforms using the NEBNext® DNA Sample Prep Master Mix Set 1 (New England Biolabs, Ipswich, MA). First ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were prepared from 1 μg of total RNA using the NEB Next Multiplex Small RNA Library Prep Set for Illumina (NEB #E7300S and #E7580S) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: A 25 μL PCR master mix was set up as follows: 2.5 μL Standard Taq (Mg-free) Reaction Buffer (10x) (B9015 New England BioLabs Inc. Ipswich MA USA), 2.0 μL dNTP mix (10mM total ...
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bioRxiv - Immunology 2021Quote: Small RNA libraries were generated from each RNA sample at TCAG (Toronto, Canada) using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, United States) and sequenced on an Illumina HiSeq 2500 rapid-run flow cell to generate 50 nucleotide (nt ...
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bioRxiv - Plant Biology 2022Quote: ... from leaves was isolated for small RNA library construction using NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... One microgram of total RNA per biological replicate was employed for the construction of 50 bp single-end (SE) reads using NEBNext® Multiplex Small RNA Library Prep Set for Illumina™ (New England Biolabs, Inc.). Lastly ...
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bioRxiv - Molecular Biology 2023Quote: ... All treated RNA samples were used for library preparation using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB catalog # E7300/7580) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... the RNA pellet was resuspended in 3’ ligation reaction buffer 1x and subjected to library preparation using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB, #E7330S). Specific primers for V ...
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bioRxiv - Genomics 2022Quote: Small RNA libraries were prepared for each sample using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... all the extracted mRNA was used to create cDNA libraries with the help of the NEBNext mRNA Library PrepReagent Set for Illumina (New England Biolabs, Ipswich, MA USA). Sequencing of the cDNA libraries was performed on the Illumina HiSeq 2500 platform (Illumina Sequencing ...
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bioRxiv - Genomics 2021Quote: ... 1μl RT Adapter (110nM; ONT Kit) and 1.5μl T4 DNA Ligase (2M U/ml New England BioLabs). The ligation reaction was carried out for 10min at RT ...
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bioRxiv - Plant Biology 2019Quote: ... ZymoResearch) and reverse-transcribed in the presence of random hexamers (LunaScript®RT SuperMix Kit, NEB Inc.) in 20 μl of total reaction volume containing 1 μg of total RNA and incubated at 55°C for 15 minutes following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... RNA was then reverse transcribed to cDNA using LunaScript RT SuperMix kit (Catalog # E3010L, New England BioLabs). w1118 flies might have partial or truncated transcripts of w+ ...
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bioRxiv - Bioengineering 2020Quote: ... We prepared cDNA from 1 μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB, #E3010). No Template and No Reverse Transcriptase controls (NTC and NRT ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated for all serially diluted RNA samples using LunaScript RT SuperMix Kit (New England BioLabs). Sufficient volume was prepared to perform duplicates for each method at each dilution ...
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bioRxiv - Microbiology 2021Quote: ... purified RNA (8 μl) was converted into cDNA using a LunaScript RT SuperMix Kit (New England Biolabs) and used as a template in two separate amplification reactions generating odd- and even-numbered tiled amplicons ...
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bioRxiv - Molecular Biology 2021Quote: ... One μg of RNA was used to prepare cDNA using the LunaScript RT SuperMix (New England Biolabs). cDNA was then diluted 10-fold and 1 μL was used per qRT-PCR reaction ...