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Citations for New England Biolabs :
1551 - 1600 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cdc11a T62A was generated using the Q5-site directed mutagenesis kit (NEB).
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bioRxiv - Biophysics 2020Quote: We used in vitro mutagenesis (Q-5 Site-directed Mutagenesis Kit, NEB) to introduce the mutations creating d isoforms using the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was generated using Postscript II 1st strand cDNA synthesis kit (NEB). Coding sequences were PCR amplified from cDNA or vectors with Q5 high-fidelity Taq Polymerase (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... or NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) was used for library construction with 500 ng of treated RNA ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Microbiology 2022Quote: ... Affinity tags were introduced by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) into the expression vectors encoding Rae1 and Nup98 using primers in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Cleanup IVT reaction using Monarch RNA cleanup kit (New England BioLabs – T2040L), eluting in 12 μL NFW ...
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bioRxiv - Immunology 2022Quote: RNA from MΦ was isolated using Monarch total RNA MiniPrep kit (BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted with the Monarch Total RNA Miniprep Kit (NEB #T2010), following the manufacturer’s indications ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized using LunaScript® RT SuperMix Kit (New England Biolabs). Gene expression to validate RNA-seq analysis was quantified by qRT-PCR using Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... and then cleaned up using the Monarch PCR Cleanup kit (T1030L, NEB). Digested PCR products were then ligated to the digested pcs2+MCS-P2A-sfGFP plasmid using T4 DNA ligase (M0202S ...
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bioRxiv - Microbiology 2022Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described in the instructions with minor modifications ...
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bioRxiv - Genomics 2022Quote: ... PCR products were purified by gel extraction (Monarch gel extraction kit, NEB), and assembled into the backbones using NEB HiFi DNA Assembly master mix kit ...
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bioRxiv - Genetics 2022Quote: ... The NEBuilder High-Fidelity DNA Assembly Cloning Kit (New England Biolabs, E5520) was used to assemble DNA fragments into the targeting construct based on manufacturer’s instructions with the primers detailed in Supplemental Table 1 ...
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bioRxiv - Genomics 2022Quote: ... DNA fragmented by sonication was repaired using Quick Blunting Kit (NEB: E1201S). For TA ligation ...
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bioRxiv - Biochemistry 2022Quote: ... Following which the PCR products were purified by PCR cleanup kit (NEB) and a second PCR was done with primers containing the READ1 ...
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bioRxiv - Genetics 2022Quote: ... using the Q5 site directed mutagenesis kit (New England Biolabs, catalogue # E0554S). The resulting modified LexA (mLexA ...
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bioRxiv - Microbiology 2020Quote: ... Using the standard protocol of the Q5 Site-directed Mutagenesis kit (NEB), the region of pTA1228 spanning restriction sites NdeI and BamHI was replaced with KasI yielding the new plasmid pTA1300 ...
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bioRxiv - Biochemistry 2021Quote: ... which were assembled using the Gibson Assembly Cloning Kit (New England Biolabs). Recombinant HWT and HI473D were expressed in E ...
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bioRxiv - Developmental Biology 2021Quote: ... Capped and tailed mRNAs were purified by Monarch RNA Cleanup Kit (NEB) according to the manufactory guide and eluted in DEPC-treated water ...
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bioRxiv - Microbiology 2021Quote: Cloning was performed using NEBuilder HiFi DNA Assembly kit (NEB, cat # E2621). Both shuttle vectors were propagated in E ...
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bioRxiv - Biochemistry 2021Quote: ... The two fragments were ligated using a DNA HiFi assembly kit (NEB). All plasmid constructs were confirmed by sequencing (Genewiz).
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bioRxiv - Biochemistry 2021Quote: ... and immunoprecipitation was performed using the EpiMark N6-methyladenosine Enrichment Kit (NEB) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... PCR products were purified by gel extraction (Monarch gel extraction kit, NEB), and assembled in a Golden Gate assembly containing 6.25 ng pU6-atgRNA-GG-acceptor (Addgene #132777) ...
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bioRxiv - Microbiology 2021Quote: ... and were ligated into pAY5211 employing the Quick LigationTM Kit (NEB, USA), resulting in the targeting plasmid (pTargeting) ...
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bioRxiv - Microbiology 2021Quote: ... Total RNAs was extracted and purified using Monarch RNA isolation kit (NEB), treated with DNase I (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... the NEBNext Ultra II DNA Prep Kit for Illumina (New England Biolabs) was used using according to the manufacturer guidelines and then sequenced using an Illumina NovaSeq6000 for 150bp PE reads.
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bioRxiv - Molecular Biology 2022Quote: ... the NEBNext Ultra II DNA Prep Kit for Illumina (New England Biolabs) was used to prepare the samples for next-generation sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated and purified using Monarch Total RNA Miniprep Kit (Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB # E7770) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated using the Monarch Genomic DNA Purification Kit (NEB) Sample Lysis ...
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bioRxiv - Developmental Biology 2022Quote: ... Fragments were then assembled using the NEBuilder HiFi DNA assembly kit (NEB) as instructed into XhoI/EcoRI or AgeI/EcoRI linearized pMT-His-V5 backbone ...
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bioRxiv - Molecular Biology 2020Quote: ... Library preparation (NEBNext Ultra RNA Library Prep kit, New England Biolabs®) and sequencing (HiSeq ...
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bioRxiv - Systems Biology 2022Quote: SDM was performed using the Q5 Site-Directed Mutagenesis Kit (NEB #E0554S) according to the manufacturer’s directions ...
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bioRxiv - Genetics 2022Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sgRNA was synthesized using HiScribe T7 High Yield RNA Synthesis Kit (NEB). The PCR fragment present the T7 promoter at 5’ end and the sequence from sgRNA of S ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or cloned using the NEB® PCR cloning kit (New Englands BioLabs) to obtain haplotype resolution ...
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bioRxiv - Genomics 2022Quote: ... We used the Q5 Hot Start High-Fidelity kit (New England Biolabs) with 10 PCR cycles for amplification ...
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bioRxiv - Microbiology 2022Quote: ... and plasmid DNA was isolated using Monarch plasmid miniprep kit (NEB #T1010).
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bioRxiv - Immunology 2022Quote: ... purified by agarose gel isolation (Monarch DNA Gel Extraction Kit, NEB (T1020L)) and ligated into LMP-destGFP (Lindner et al ...
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bioRxiv - Immunology 2022Quote: ... The circRNA products were further purified using Monarch RNA Cleanup Kit (NEB). The quality and quantity of RNA were evaluated by agarose gel electrophoresis and Qubit RNA HS Assay Kit (Invitrogen).
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Orchestration of alternative splicing regulates bone marrow mesenchymal stem cells fate during agingbioRxiv - Cell Biology 2022Quote: ... NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) was used to generate sequencing libraries according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: The NEBNext Ultra II directional RNA library prep kit for Illumina (NEB) was used to build cDNA libraries in preparation for sequencing ...
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bioRxiv - Genetics 2020Quote: ... Amplicons were purified using the Monarch® PCR & DNA Cleanup Kit (NEB) following the manufacturer’s instructions and sent for 250 bp paired end Illumina amplicon sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2020Quote: ... ribosomal RNA was depleted with the NEBNext rRNA Depletion Kit (NEB, #E6350). Libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Biochemistry 2020Quote: The mutagenesis was performed using Q5® Site-Directed Mutagenesis Kit (NEB) according to manufacturer’s instructions using primers listed in Extended Data Table 1.
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bioRxiv - Developmental Biology 2020Quote: ... and reverse transcribed using ProtoScript II first strand cDNA synthesis kit (NEB), following the manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... Decrosslinked DNA was recovered using the Monarch PCR DNA Cleanup Kit (NEB), eluted in 50 µl of elution buffer and analysed by qPCR using a LightCycler 480 instrument (Roche) ...
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bioRxiv - Biophysics 2021Quote: ... RNA was generated using the HiScribe T7 Quick kit (New England Biolabs) and extracted using trizol and isopropanol precipitation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: We used the Q5 site-directed mutagenesis kit (NEB, Ipswich, MA #E0552S) to generate mutations in the ECL2 of human ADGRG2 ...