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Citations for New England Biolabs :
1701 - 1750 of 10000+ citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cleaved protein product was passed through Ni-NTA resin and amylose resin (E8021, NEB) to remove the TEV protease and HIS-MBP tag ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant proteins were then incubated with MBP amylose magnetic beads (New England Biolabs) for 1 h at 4°C and washed with 200 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: For lambda phosphatase assay the reactive from Lambda Protein Phosphatase (Lambda PP) (P0753L, NEB) were used and followed the protocol from the manufacturer.
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bioRxiv - Plant Biology 2024Quote: ... The protein samples were treated with Endo Hf (New England BioLabs, Ipswich, MA, USA) at 37°C for 1 h ...
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bioRxiv - Genomics 2022Quote: ... and ribosomal RNAs were depleted using ribosomal RNA depletion kit (rRNA Depletion Kit, NEB). Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Molecular Biology 2024Quote: ... purified using column DNA Clean-up kit (Monarch PCR & DNA Cleanup Kit, # T1030L, NEB) and mixed with Nhe1 and BamH1 double-digested and gel-purified target pCW57.1 vector in a molecular ratio of 1:2 ...
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bioRxiv - Developmental Biology 2021Quote: ... using HiScribe Sp6 kit (NEB) instead of T7.
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bioRxiv - Neuroscience 2021Quote: ... rRNA depletion kit (NEB #E6310), and Ampure XP beads (Beckman #A63881) ...
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bioRxiv - Microbiology 2020Quote: ... the PNGase kit from (NEB) was used per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... NEBNext rRNA Depletion kit (NEB) was used to remove bacterial and eukaryotic rRNA from total RNA isolated from pooled samples ...
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bioRxiv - Genetics 2022Quote: ... HiFi Assembly Cloning Kit (NEB) to insert the LYP1 homology sequence into the BamHI/EcoRV digest of Addgene plasmid #35121 to create the final backbone plasmid BFA0190 (Supplementary Figure 10) ...
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bioRxiv - Genomics 2022Quote: EnGen sgRNA synthesis kit (NEB)
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bioRxiv - Genomics 2022Quote: Monarch RNA Cleanup kit (NEB)
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bioRxiv - Microbiology 2022Quote: ... LunaScriptTMSuperMix Kit (New England BioLabs), SYBR qPCR Master Mix (ChamQTMUniversal ...
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bioRxiv - Microbiology 2022Quote: ... or NEBase Changer–Kit (NEB) and primers listed in supplementary Table 1 ...
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bioRxiv - Genetics 2022Quote: ... Gibson assembly cloning kit (NEB) was applied to ligate vector and GFP∷PAB-1∷3xFLAG fragment to generate plasmid pQZ2 ...
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bioRxiv - Microbiology 2021Quote: ... The A-Tailing Kit (NEB) was then used to add additional deoxyadenosine (A ...
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bioRxiv - Neuroscience 2022Quote: ... Quick Ligation Kit (M2200L, NEB) was used for the ligation of these appropriate clone ...
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bioRxiv - Biochemistry 2022Quote: ... using Q5 mutagenesis kit (NEB). N-(-42D ...
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bioRxiv - Cell Biology 2022Quote: ... using Gibson Assembly kit (NEB). To test the interaction of KRP4 and KRP5 ...
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bioRxiv - Microbiology 2022Quote: ... LunaScript RT SuperMix Kit (NEB) was used to generate cDNA using 1μg RNA ...
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bioRxiv - Plant Biology 2023Quote: ... blunting (Quick Blunting kit, NEB), and re-ligating using T4 DNA Ligase (NEB).
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bioRxiv - Genomics 2023Quote: ... or RNA cleanup kit (NEB) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Q5 DNA polymerase kit (NEB) was used to make amplicons ...
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bioRxiv - Biophysics 2023Quote: ... Gibson Assembly Kit (#E5510S, NEB) was used following the manufacturer’s instruction ...
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bioRxiv - Synthetic Biology 2023Quote: Monarch Plasmid MiniPrep kit (NEB) for isolating template plasmid and assembled plasmid from E ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA prep kit from NEB, and all other reagents from Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... The resulting fragment was gel-purified and then used to be transcribed using either MegaScript kits from Ambion or HiScribe kits from NEB according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Electroligase kit (NEB). Electrocompetent ElectroMax Stbl4 bacteria (Invitrogen ...
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bioRxiv - Plant Biology 2019Quote: Genomic regions containing each of the TIR1/AFB genes were amplified using Phusion polymerase (New England Biolabs or ThermoFisher) from corresponding genomic clones (JAtY51F08 ...
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bioRxiv - Biophysics 2021Quote: ... Each fragment was produced by PCR using primers containing the restriction recognition sites for enzymes BbsI and BsaI (NEB), which recognize asymmetric DNA sequences and cut outside of their recognition sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was initiated by diluting the LbCas12a complexes to a final concentration of 37 nM LbCas12a : 37 nM sgRNA in a solution containing 1X NEBuffer 2.1 (NEB) and 1 µM custom ssDNA reporter substrates in a 40 μL reaction volume ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 µl of 1xMNase buffer containing 60 gel units/ml (6 Kunz units/ml) of Micrococcal nuclease (NEB M0247S) were added and incubated for exactly for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... The amplified plasmid containing the mutated p66 was double digested with NdeI and XhoI restriction enzymes (New England Biolabs), gel purified and cloned into MCS-2 of the pETDuet-1 vector pre-digested with the same set of restriction enzymes and carrying mutated p51 domain in MCS-1 ...
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bioRxiv - Genomics 2022Quote: ... with a minimal promoter and partial 3’ UTR was amplified from pMPRAduo:minP:GFP (addgene pending) in 1600 μL volume containing 800 μL of Q5 High-Fidelity 2X Master Mix (NEB, M0492L) and 0.5 μM forward and reverse primers (primers 7 and 8 for vector A and primers 9 and 10 for vector P ...
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bioRxiv - Genomics 2020Quote: Cas9 protein and transcribed sgRNA were incubated for 10 min at room temperature in reaction buffer containing 1× NEB buffer 3.1 (NEB Biolabs) supplemented with 1 mM DTT ...
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bioRxiv - Microbiology 2019Quote: ... Amplification was performed in a total volume of 25 µl containing 12.5 µl of 2x OneTaq Mastermix (New England Biolabs, NEB), 9.9 µl of nuclease-free H2O ...
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bioRxiv - Synthetic Biology 2020Quote: ... and subsequently mixed at a 6:1 ratio with the digested backbone in reactions containing T4 DNA ligase (NEB).
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bioRxiv - Genetics 2019Quote: ... the MX-PCR was performed with a total reaction volume of 25 μL containing 12.5 μL Phusion Hot Start Flex 2X Master Mix (New England BioLabs), 25 ng of K562 cell line genomic DNA and 5 μL of the primer pool as defined in Supplementary Table 3 ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was amplified in the region containing the expected KMT5C mutation using Q5 high fidelity polymerase (M0491L, NEB). Primers for amplification and sequencing are outlined in Supplementary Table 3.
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μl of reaction was added to a tube containing 5 μl replication buffer and 1 μl MseI (NEB) for 3 min ...
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bioRxiv - Plant Biology 2020Quote: ... 100ng of each entry and destination vector are assembled in one reaction mix containing 10U BsaI-HF-v2 (NEB), 200U T4 Ligase (NEB) ...
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bioRxiv - Genetics 2020Quote: ... the suspension was then mixed with 200 μl of master mix containing 50 μl 10x T4 ligase buffer (NEB), 2.5 μl 20 mg/ml BSA ...
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bioRxiv - Genetics 2022Quote: ... Eggs were then injected under air-dry conditions with a solution containing 300ng/μl of recombinant Cas9 Nuclease (NEB) and 100ng/ul each of four sgRNAs targeting the first exon of the Oatp74D gene (S2 Table) ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellets containing intact nuclei were vortexed at maximum setting for 15 sec with the nuclear extraction buffer (NEB) and then incubated at 4°C for 30 min with gentle mixing ...
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bioRxiv - Immunology 2022Quote: ... T cell clones were thawed and allowed to rest at 37°C in RPMI containing DNAse (5µg/mL, New England Biolabs).
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bioRxiv - Genomics 2022Quote: ... Cells were fixed with 3% paraformaldehyde in PBS for 10 min at room temperature and permeabilized for 5 min on ice in PBS containing 0.5%Triton X-100 and 2mM Vanadylribonucleoside complex (New England Biolabs). Coverslips were preserved in 70% EtOH at -20°C ...
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bioRxiv - Neuroscience 2020Quote: ... soluble supernatants were immunopreciptated with monoclonal anti-GFP antibody precoupled to Protein G Sepharose beads and immunoprecipitates were washed extensively with IPB containing 0.5M NaCl followed by further washes with IPB and then with lambda phosphatase assay buffer (NEB). Equal volumes of each immunoprecipitated sample were then aliquoted into two tubes and beads were then resuspended with 100 microliters of lambda phosphatase assay buffer containing 400 units of lambda protein phosphatase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were then washed once in 5 volumes of binding buffer (100μL, 1xPBS containing 1mg/mL of BSA (NEB)) for 10 minutes at RT ...