Labshake search
Citations for New England Biolabs :
1601 - 1650 of 6282 citations for 6 Quinolinamine 1 ethyl 1 2 3 4 tetrahydro 2 2 4 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: GFP coding sequence was amplified from plasmid p2GWF7 (Karimi et al., 2002) using GFPF40 and GFPR40 primers (see Supplemental Method 2) with Phusion polymerase (New England BioLabs) according to the provider’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids were fully linearized by incubation at 37°C for a minimum of 2 hours using ClaI restriction enzyme (New England Biolabs). Full-length genomic RNA was transcribed from the linearized plasmids using the MegaScript T7 Kit (Ambion) ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 µl of 2 U/µl Phusion polymerase and 160 µl of 40 U/µl Taq ligase (all enzymes from NEB). ddH2O was then added to a final volume of 1.2 ml.
-
bioRxiv - Biophysics 2021Quote: SARS-CoV-2 S N501Y plasmid was obtained from SARS-CoV-2 S plasmid (HDM-IDTSpike-fixK) by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs). SARS-CoV-2 S and SARS_CoV-2 S N501Y pseudotyped retroviral particles were produced in HEK293T cells as described previously (29) ...
-
bioRxiv - Biophysics 2020Quote: ... The construct was amplified by PCR and inserted into pSNAP-tag®(T7)-2 vector (New England Biolabs Inc. #N9181S) with a SNAPf-EGFP-6His cassette ...
-
bioRxiv - Cell Biology 2022Quote: ... 100 μg of tissue or cell lysates were treated with or without 2 μL Lambda Protein Phosphatase (LPP) (New England Biolabs) at 30 °C for 15 min (30 min for HEK-293E lysate ...
-
bioRxiv - Developmental Biology 2022Quote: ... PE3-EGFP-2-Fw and PE3-EGFP-2-Rv) were annealed and inserted into the BsmBI sites of BPK1520 using a Quick Ligation Kit (New England BioLabs). PuroR cDNA was amplified from PX459 with primers (PuroR-Fw ...
-
bioRxiv - Biochemistry 2022Quote: ... His-MBP-tagged proteins were first purified by Ni-NTA affinity chromatography as above and then applied for 2 h to an amylose agarose matrix (NEB). Bound proteins were eluted with wash buffer containing 20 mM maltose ...
-
bioRxiv - Molecular Biology 2022Quote: ... A volume of cDNA corresponding to 2.5 ng of the input RNA was subjected to 35 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
-
bioRxiv - Molecular Biology 2022Quote: ... and mixed with a master mix consisting of 20 μl of 2 x NEBNext Ultra II Q5 Master Mix (NEB), 1 μl of 40 μM P5* primer ...
-
bioRxiv - Molecular Biology 2022Quote: ... and a volume of cDNA corresponding to 5 ng of the input RNA was subjected to 30 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
-
bioRxiv - Microbiology 2022Quote: ... Nsp1 and 2 genes were cloned into pENTR (L1-L2) using Hifi DNA Assembly (New England Biolabs, Ipswich, MA, USA). LR recombination Gateway (ThermoFisher Scientific ...
-
bioRxiv - Biophysics 2022Quote: SNAP-tag labeling of SNAP-mGluR2 was done by incubating cells with 2 µM of SNAP-Surface Alexa Fluor 549 (NEB) and 2 µM of SNAP-Surface Alexa Fluor 647 (NEB ...
-
bioRxiv - Biochemistry 2022Quote: ... and cloned in the EcoRV and BamHI restriction site of the pBBR1MCS-2 (55) using the NEBuilder Assembly kit (New England BioLabs). pBBR1MCS-2 was a gift from Kenneth Peterson (Addgene plasmid # 85168 ...
-
bioRxiv - Microbiology 2021Quote: ... The DNA chimera was then cloned into the pACP-tag (m)-2 vector (addgene# 101126) using NheI and NotI (NEB) as the restriction sites ...
-
bioRxiv - Microbiology 2020Quote: ... the V1-V3 region of 16S rRNA genes was amplified from 2 ng of DNA in 50 μl reactions containing the following components: 1x Standard Taq Reaction Buffer (NEB), 3 mM MgCl2 (NEB) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The ends of DNA were repaired by incubating in 70 μL of 1X NEBuffer 2 containing 0.6 units of T4 DNA polymerase (NEB, M0203L), 2 units of T4 polynucleotide kinase (NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... The new SNAP-tagged histones synthesized during the chase were fluorescently labelled with 2 µM of the red-fluorescent reagent SNAP-cell TMR star or SiR-647 (New England Biolabs) during a 15 min-pulse step followed by 30 min wash in fresh medium ...
-
bioRxiv - Microbiology 2020Quote: ... 20 μg of recombinant PbChiB2 and PbChiB4 resuspended in 20 mM Tris (pH 8.0) were independently mixed with 2 mg of chitin magnetic beads (NEB, CAD), chitin from shrimp shell (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: ... double-strand DNA repair templates were amplified with PCR from the plasmids pSL779 for gfp or pSL780 for mKate2 using Phusion polymerase and the primers listed in table 2 (New England Biolabs) (Bone ...
-
bioRxiv - Immunology 2020Quote: ... the DNA ladder (Quick-Load® Purple 2-Log DNA ladder 0.1-10 kb, #N0550S) were provided by New England Biolabs (NEB), The polymerase enzyme (Accustart Taq DNA polymerase HiFi ...
-
bioRxiv - Molecular Biology 2022Quote: ... These 2 fragments were assembled into pCOM1 (Hayashi et al., 2016) at Van91I sites by Gibson assembly (New England Biolabs). The assembled plasmid ...
-
bioRxiv - Immunology 2022Quote: ... 2018” tagmentation mix were either sorted into plates containing RCB buffer for condition “hiSDSprotK-TWEEN” (2 x RCB: 100 mM Tris-HCl pH 8, 100 mM NaCl, 40 µg/mL Proteinase K (NEB), 0.4 % SDS (Sigma ...
-
bioRxiv - Cell Biology 2022Quote: ... 100 μl of MBP-SYCP1N at 2 mg/ml was incubated for 20 minutes on ice with 100 ul amylose resin slurry (NEB) equilibrated in 20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... and inserted into the pSB_Avi_3×FLAG_insert_mScarlet_Puro vector (linearized by AsiSI and NotI) using Gibson assembly based on protocol instructions of 2×HiFi master mix (New England Biolabs). The correct constructs were selected based on Sanger Sequencing results (Eurofins).
-
bioRxiv - Synthetic Biology 2019Quote: 2 μL of the Dynabeads were used as template for the final PCR using barcoded P5 and P7 primers and Q5 HiFi 2× Master Mix (NEB) + SYBR Green ...
-
bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions (2×1012 particles/ml) were incubated in the presence of 10 μg/ml endoproteinase LysC (New England Biolabs) at 37°C in a S1000 thermal cycler (Bio-Rad) ...
-
bioRxiv - Biochemistry 2019Quote: ... Washed resins were resuspended in 1 CV of buffer A and mixed with 10xHis-tagged Gi heterotrimer at the ratio of 2 mg Gi per ml resin with the presence of 25 mU/ml apyrase (New England Biolabs). The resuspended resin/Gi mixture was subjected to irradiation with 495-nm long-passed filter for 10-15 minutes to induce activation of rhodopsin and G protein binding ...
-
bioRxiv - Molecular Biology 2020Quote: ... Ligation Test 1 was processed directly to index PCR while product in Ligation Test 2 was digested by 12.5 units of RNase H (NEB, Cat.No.M0297) at 37°C for 20min before index PCR.
-
bioRxiv - Microbiology 2019Quote: High throughput sequencing libraries were prepared using RNA isolated from PAR-CLIP samples using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2; cat# E7580, New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... We treated DNA extractions with 2 μl of RNAase A (ThermoScientific) and purified them with Monarch PCR & DNA Cleanup Kit (New England Biolabs). We assessed DNA quantity and quality with Qubit 3.0 (Thermofisher) ...
-
bioRxiv - Immunology 2019Quote: ... Restriction enzyme digests were then performed using bsII at 55° for 2 hours (for cxcr4b) and bstXi (New England Biolabs) at 37° for 2 hours (for cxcl12a) ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were washed once more with PBS and reincubated with complete medium with 2 μM BTP (SNAP-Cell Block; New England Biolabs) for 30 minutes and again washed with PBS and further treated for analysis ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by annealing and ligation with a DNA/RNA-hybrid stem-loop adapter using T4 RNA ligase 2 (New England Biolabs), which specifically ligates the adapter to mature tRNA ...
-
bioRxiv - Pathology 2020Quote: ... DNA was visualized on 2% agarose gel and genotypes were determined based on PCR product length quantified using a DNA ladder (NEB N0556S ...
-
bioRxiv - Neuroscience 2020Quote: ... The size of libraries was selected for cDNA target fragments of 200–300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 cycles ...
-
bioRxiv - Genetics 2020Quote: ... The RNA was then diluted 1:50 and 2 mL were used to perform a one-step qPCR protocol using Luna Universal One-step qPCR kit (NEB). Two primer sets were used ...
-
bioRxiv - Microbiology 2020Quote: ... each reaction tube of 20 μl contained 10 μl of Q5 High-Fidelity 2× Master Mix (New England BioLabs Inc.), 20 pmol of forward and reverse primer ...
-
bioRxiv - Plant Biology 2021Quote: ... One μl of each 100 μM oligo was added to 500 μl 1x NEB buffer 2 (New England Biolabs, www.neb.com). The p201N:Cas9 plasmid was linearized by digestion with Spe1 (www.neb.com ...
-
bioRxiv - Microbiology 2019Quote: ... The Illumina RA3 adapter sequence was ligated to the purified RNA using T4 RNA Ligase 2 (NEB, cat. number: M0242) for 2 hours at 25 °C and reverse transcription was performed with Reverse Transcription Primer (Illumina sequence ...
-
bioRxiv - Molecular Biology 2020Quote: ... NOCT Δ(2-15)-3F was PCR amplified from NOCT Δ(2-15)-3F pFC3F and inserted into pCW57.1 using Gibson Assembly using the NEBuilder HiFi Assembly Master Mix (NEB, E5520). NOCT (1-431)-3F pcDNA5/FRT/TO was generated through PCR amplification of NOCT (1-431 ...
-
bioRxiv - Bioengineering 2021Quote: ... which were subsequently cloned into a custom vector (Supplementary sequence 2, BfuAI and EcoR I digested) by the Gibson assembly method (NEB). To generate nicking sgRNA expression plasmids ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Integration of the sequences and removal of sacB gene was confirmed by colony PCR (SI Fig 2) with OneTaq Hot Start Quick-Load 2X Master Mix with GC buffer (New England BioLabs) using a Touchdown thermocycling protocol with an annealing temperature ranging from 72-62°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5 uL aliquots were removed at each time point and added to 2 uL 5 mg/mL Proteinase K (NEB) in 5% SDS ...
-
bioRxiv - Plant Biology 2021Quote: ... 0.3-kb promoter-xopL gene was PCR amplified using Xcv 85-10 genomic DNA as template and cloned into pBBR1MCS-2 (76) linearized with EcoRV (New England Biolabs) using Gibson assembly ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and 0.2 μl 50x oligos (AarI recognition site) for Level 0 and Level 2 cloning or Eco31I/BsaI-HFv2 (ThermoFisher Scientific/NEB) for Level 1 cloning ...
-
bioRxiv - Genomics 2019Quote: ... D7 and D5 pre-annealed indexed adapters (2 ng and 200 ng, respectively) and T4 DNA ligase (400 U, NEB), and were incubated at 16°C overnight ...
-
bioRxiv - Biochemistry 2019Quote: ... In the case of Biotinylated-AviTag-haBiP(28-635)T229A-V461F this protein was also made nucleotide free by the addition of 2 U CIP (NEB) per mg of BiP ...
-
bioRxiv - Genomics 2021Quote: ... The PCR mix (8 µl H2O, 2 µl primer mix P5Solexa/P3Solexa, 10 µM each, 20 µl Phusion HF Mix [New England Biolabs]) was added to 10 µl cDNA ...
-
bioRxiv - Genetics 2020Quote: ... containing the silent mutations to stem 2 of SARS and SARS-CoV2 (IDT) into the plasmid using T4 DNA ligase (NEB).