Labshake search
Citations for New England Biolabs :
1501 - 1550 of 3576 citations for Vanin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 1 ul of micrococcal nuclease (Biolabs M02475, 2×106 U/ml) was added to 200 μl buffer containing 1 mM CaCl2 and incubated for 10 min 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... 1X Cutsmart buffer and 1 μL of Dpn1 (New England Biolabs) was added to 25 μL of PCR product and incubated at 37 °C for 4 hours to remove template DNA ...
-
bioRxiv - Microbiology 2022Quote: ... The exon 1 targeting plasmid was digested with XhoI/KpnI (NEB), and the exon 7 guide was amplified with primers 22 and 23 to facilitate gibson cloning of the exon 7 guide into the same backbone ...
-
bioRxiv - Microbiology 2022Quote: ... 1× LongAmp® Taq 2× Master Mix (New England Biolabs, UK), and 2 μL of a unique barcode ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM DTT) and incubated with Quick CIP (NEB cat #M0525S) for ∼16 hours ...
-
bioRxiv - Genomics 2023Quote: ... 1 U/μL T4 PNK (New England Biolabs, Cat no M0201S); concentrations refer to a final volume of 50 μL] were added ...
-
bioRxiv - Genomics 2023Quote: ... and incubated with 1 μl DNase I (New England Biolabs, M0303S) and 10 μl RNase A (Thermo Scientific ...
-
bioRxiv - Plant Biology 2023Quote: ... and 1 μL of 400 U T4 DNA Ligase (NEB, M0202S). The following thermocycler program was used ...
-
bioRxiv - Plant Biology 2023Quote: ... 2.5% (v/v) 1 mg/mL bovine serum albumin (NEB #B9200S), 5% (v/v ...
-
bioRxiv - Biochemistry 2023Quote: ... with 1 unit Rnase Inhibitor (New England Biolabs Inc., Massachusetts, USA) and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co ...
-
bioRxiv - Biochemistry 2023Quote: ... with 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA) and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co ...
-
bioRxiv - Biophysics 2023Quote: ... and 5 U Klenow in 1× NEBuffer 2 (New England BioLabs) (120 μL total volume ...
-
bioRxiv - Genomics 2023Quote: ... 1 μl SUPERase RNase Inhibitor and 20 μl DdeI (NEB, R0175L). The sample was then incubated at 37 °C for 1 hr and centrifuged at 500x g for 3 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3.5 µL ddH2O and 1 µL 10 µM RTP primer (NEB) was added ...
-
bioRxiv - Biochemistry 2022Quote: ... the samples were incubated with 1 µl Exonuclease I (NEB, #M0293) and 2 µl of 10X reaction buffer at 37°C for 30 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 1 μl T4 RNA ligase 2 truncated KQ (NEB M0373L). Plugs were then rinsed with 1 ml Tris buffer ...
-
bioRxiv - Genomics 2023Quote: ... 2.5 μL of Digestion-1 mix (MseI (12.5 U, NEB, R0525M) and/ or MboI (12.5 U ...
-
bioRxiv - Cell Biology 2023Quote: ... pAG306GPD-ORF chr 1 was digested with NotI-HF (NEB, #10133990).
-
bioRxiv - Systems Biology 2023Quote: ... and 1 μL T4 DNA Ligase (400,000 U/mL, NEB, #M0202S). Ligation occurred at 25°C for 2 hours ...
-
bioRxiv - Biochemistry 2023Quote: ... template DNA was digested by incubation with DpnI (1 μl; NEB) at 37 °C for 1 hour ...
-
bioRxiv - Immunology 2023Quote: ... 1 μL of DpnI restriction endonuclease (New England Biolabs; cat # R0176L) was added to each reaction tube to digest DNA templates ...
-
bioRxiv - Immunology 2022Quote: ... 1 µl Phusion Hot Start II DNA Polymerase (2U/ µl) (NEB); 1 µl 10 mM dNTPs ...
-
bioRxiv - Genetics 2023Quote: ... Then 0.5 µL of 1 MgCl2 and 2µL RNase inhibitor (NEB) were added to each sample and mixed by vortexing ...
-
bioRxiv - Immunology 2023Quote: ... 1 mM CaCl2 and treated with N-glycanase (New England Biolabs) for 1 hour at 37°C.
-
bioRxiv - Cell Biology 2023Quote: ... cells were stained with 1 µM SNAP-cell SiR-647 (NEB) for 30 min at 37 °C and 5 % CO2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 μl of dNTPs (10 mM of each – New England Biolabs), 1 μl of iTP_Linker_r oligonucleotide (2 μM) ...
-
bioRxiv - Biochemistry 2023Quote: ... and probed with either anti-histone H3 (1:1000, NEB, 9715S) or anti-histone H3 (phosphor S10 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 1 µL of each Exonuclease I (NEB, cat. no. M0293S) and Exonuclease III (NEB ...
-
bioRxiv - Developmental Biology 2023Quote: ... 72°C for 1 min) using NEBNext 2x Master Mix (NEB) and Nextera adapters and put on ice ...
-
bioRxiv - Cell Biology 2023Quote: ... and 200 µg/ml RNAse A (New England Biolabs #T3018-1)) for four hours on ice ...
-
bioRxiv - Neuroscience 2023Quote: ... and 1 μl of Lambda protein phosphatase (P0753L, New England BioLabs) were incubated with the proteins at 30°C for 30 minutes.
-
bioRxiv - Molecular Biology 2023Quote: ... and 1 U of Taq Standard Polymerase (New England Biolabs #M0273) in a final volume of 30 μL ...
-
bioRxiv - Microbiology 2023Quote: ... the elute was recovered by adding 1 μl liner acrylamide (NEB) and precipitating in ethanol at -80°C for 1 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... Each 20 µL PCR reaction contained 1× OneTaq Master Mix (NEB), 0.2 µM of each primer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and 1 U of Taq DNA polymerase (OneTaq, New England Biolabs). The PCR conditions included an initial denaturation step at 94°C for 3 min ...
-
bioRxiv - Cell Biology 2023Quote: ... 1mM DTT) before adding 15 µl 1 mg/ml Streptavidin (NEB) before immediate wash with DLB-C-T buffer (DLB supplemented with 1 mg/ml α-casein ...
-
bioRxiv - Molecular Biology 2023Quote: ... or 8 µl RNase III (1:1000 diluted, New England Biolabs) or RNase If (1:100 diluted ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of Golden Gate Enzyme Mix (BsaI-HFv2, NEB M2616AA), and adding dH2O to reach a total volume of 20 µl ...
-
bioRxiv - Microbiology 2024Quote: ... Ligation of 5’ linker using T4 RNA ligase 1 (NEB; #M0204S) was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB ...
-
bioRxiv - Bioengineering 2024Quote: ... NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB, 7335S) were used for the final PCR amplification ...
-
bioRxiv - Microbiology 2024Quote: ... respectively) using T4 RNA Ligase 1 (New England Biolabs, USA; #M0204) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... made up of 1 uL DNA Ladder (New England Biolabs, N3231L), 1 µL Purple Dye (New England Biolabs ...
-
bioRxiv - Molecular Biology 2024Quote: ... 72 °C for 1 min) × 5] using NEBNext 2xMasterMix (NEB, M0541S) with Ad1_noMX and v2_Ad2.* indexing primers followed by qPCR amplification to determine additional cycle numbers ...
-
bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog #M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
-
bioRxiv - Molecular Biology 2024Quote: ... steps 1–3: GELase was replaced by β-agarase I (NEB). The reaction (1 U per plug ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 1 μl M13KO7 helper phage (NEB).
-
bioRxiv - Microbiology 2024Quote: ... (b) Heat labile Shrimp Alkaline Phosphatase (rSAP) (1 µl) (NEB #M0371S) was used to remove terminal phosphates that could interfere with terminal extension by incubating in rCutSmart buffer (3.5 µl ...
-
bioRxiv - Systems Biology 2024Quote: ... IVT products were treated with 1 U of DNase I (NEB) at 37°C for 15 min and brought to a final volume of 100 µL with nuclease-free water ...
-
bioRxiv - Genomics 2024Quote: ... 16ul nuclease-free water and 1 μl T4 DNA ligase (NEB). The entire volume of the reaction was pipetted on a parafilm sheet placed on a glass plate and the coverslips were inverted over it ...
-
bioRxiv - Genomics 2024Quote: ... Unmethylated λ-bacteriophage DNA (1:200 mass ratio; New England Biolabs) was included in all samples to calculate unmethylated cytosine conversion efficiency ...