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Citations for New England Biolabs :
101 - 150 of 385 citations for n1 Alpha L arabinopyranosylamino guanidine hno3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... NEB Stable (lentiviral vectors) and NEB 5-alpha (other plasmids) (New England Biolabs) were used as cloning strains ...
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bioRxiv - Cell Biology 2023Quote: ... and N1 mice from these crosses were genotyped as above using a proof-reading polymerase (NEB Q5 DNA Polymerase). Purified amplicons from these PCR reactions were then Sanger sequenced to ensure accuracy of insertion site and genomic sequence ...
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bioRxiv - Neuroscience 2023Quote: ... pSNAP-N1 was generated in-house by Hannes Gonschior by sub-cloning pSNAPf (New England BioLabs Inc., Cat#N9183S) via PCR and the restriction enzymes AgeI and NotI into pmCherry-N1 ...
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bioRxiv - Neuroscience 2023Quote: ... This was confirmed in N1 heterozygous AtrxR245C/+ females after digestion of the wild-type allele using FspI (NEB R0135S) and gel purification of the uncut mutant band ...
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bioRxiv - Microbiology 2022Quote: Escherichia coli DNA adenine methyltransferase (dam)+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/dcm− (dam−/dcm− competent E ...
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bioRxiv - Microbiology 2021Quote: ... coli transformations were performed using NEB 5-alpha chemically competent cells (New England BioLabs). E ...
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bioRxiv - Genomics 2024Quote: ... Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB) and grown in liquid LB-Amp cultures ...
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bioRxiv - Microbiology 2023Quote: ... All the produced constructs were propagated in competent cells (5-alpha Competent E.coli; NEB) and isolated by NucleoSpin Plasmid (TaKaRa) ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of S proteins were digested respectively using alpha lytic protease (New England BioLabs), chymotrypsin (Athens Research and Technology) ...
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bioRxiv - Biochemistry 2024Quote: ... The assembly reaction was transformed into NEB 5-alpha competent cells (New England Biolabs). After the recovery step ...
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bioRxiv - Molecular Biology 2024Quote: ... coli 5-alpha and purified using the Monarch Plasmid Miniprep Kit (New England Biolabs).
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bioRxiv - Synthetic Biology 2024Quote: ... Transformations used the NEB recommended protocols for chemical transformation (NEB 5-alpha, Cat# C2987H) or electroporation (NEB 10-beta ...
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bioRxiv - Synthetic Biology 2024Quote: ... Constructed plasmids were transformed and maintained in NEB 5-alpha (New England Biolabs (NEB) C2987H ...
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bioRxiv - Synthetic Biology 2024Quote: ... Constructed plasmids were transformed and maintained in NEB 5-alpha (New England Biolabs (NEB) C2987H ...
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bioRxiv - Biophysics 2022Quote: Saturation mutagenesis of mEosEM at His63 was performed in the pEGFP-N1-mito-mEosEM plasmid with Q5 high fidelity polymerase (New England Biolabs). The amplified fragments containing homologous arms and mutation sites were transformed into the Top10 competent cells (Tsingke ...
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bioRxiv - Cell Biology 2022Quote: pHALO-C1 and pHALO-N1 were generated by replacing the eGFP in Clontech vectors with HALO using Gibson assembly (E2621L; New England Biolabs). HALO-ARHGEF10 ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting PCR fragment and the peGFP-N1-1 vector were sequentially digested with SalI and SacII prior to ligation with Quick ligase (New England Biolabs) to make a pcachd1-eGFP-N1-1 plasmid ...
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bioRxiv - Cell Biology 2023Quote: pHaloTag-C1 and pHaloTag-N1 were generated by replacing the eGFP in Clontech vectors with HaloTag using Gibson assembly (E2621L; New England Biolabs). HaloTag-ACBD3 ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli using standard transformation techniques with NEB 5-Alpha chemically competent cells (New England BioLabs), and to C ...
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bioRxiv - Synthetic Biology 2023Quote: All cloning was performed in NEB 5-alpha Competent Escherichia coli (New England BioLabs C2987U). The Pseudomonas putida KT2440 strain was purchased from ATCC (#47054) ...
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bioRxiv - Molecular Biology 2023Quote: ... The assembled plasmid was introduced into Escherichia coli strain NEB 5-alpha (New England Biolabs) by heat shocking ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′ Iq competent Escherichia coli (NEB, Cat# C2992H) and extracted using the PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were amplified in chemically competent 5-alpha F’Iq Escherichia coli cells (New England Biolabs) after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... plasmids were chemically transformed into DH5-alpha cells (cat no. C2987, New England Biolabs Inc.) and plated on agar plates containing appropriate antibiotics ...
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bioRxiv - Genetics 2024Quote: All plasmids were transformed into NEB 5-alpha Competent Escherichia coli cells (New England Biolabs) and purified using Qiaprep Spin Miniprep Kits or Plasmid Plus Maxi Kits for plasmid libraries (QIAGEN) ...
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bioRxiv - Biophysics 2024Quote: ... The ligation mix was then transformed into NEB 5-alpha competent cells (NEB, Cat. # C2987H) and colonies were selected using 50 µg/mL Kanamycin LB agar plate ...
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bioRxiv - Molecular Biology 2020Quote: ... The obtained pri-miRNA sequences were cloned into the pEGFP-N1 backbone vector by using AgeI and XbaI restriction enzymes and T4 DNA ligase (NEB BioLabs, USA). For shRNA overexpression ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR inserts were assembled with restriction enzyme digested pmCherry-N1 backbone in Gibson Assembly Master Mix (New England Biolabs; E2611S) as per manufacturer recommendations and the assembled plasmids transformed into NEB5a cells (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... The APOE3 ORF was amplified from APOE3-TurboGFP and subcloned into an mEmerald-N1 backbone via Gibson assembly using HiFi DNA Assembly Master mix (New England Biolabs, E2621) to make APOE3-mEm ...
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bioRxiv - Cancer Biology 2024Quote: ... 60 containing human RNaseH1 with D210N catalytic dead mutant was amplified by PCR for cloning in pEGFP-N1 using EcoRI (NEB, #R3101S) and KpnI (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... and the insert was cut out and ligated into pEGFP-N1 plasmid prepped from a dam- dcm- E.coli strain (New England Biolabs Cat# C2925H) and digested with NheI and XbaI ...
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bioRxiv - Bioengineering 2020Quote: ... Esp31 (NEB R0734S/L), and T7 DNA Ligase (NEB M0318S/L ...
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bioRxiv - Bioengineering 2020Quote: ... Esp31 (NEB R0734S/L), and T7 DNA Ligase (M0318S/L) ...
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bioRxiv - Bioengineering 2020Quote: ... Esp31 (NEB R0734S/L), and T7 DNA Ligase (M0318S/L ...
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bioRxiv - Microbiology 2024Quote: ... Murine (NEB, #M0314S/L) and nuclease-free water at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The obtained pri-miRNA sequences were cloned into the pEGFP-N1 backbone vector by using AgeI and XbaI restriction enzymes and T4 DNA ligase (NEB BioLabs, USA). For shRNA overexpression ...
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bioRxiv - Cancer Biology 2021Quote: ... 5uL of the purified product was transformed using DH5-alpha Electrocompetent E.coli (NEB, Cat. No. C2986) on a Eppendorf Eporator® (Eppendorf ...
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bioRxiv - Cell Biology 2024Quote: ... Background assembly was measured by transformation of NEB 5-alpha competent cells (New England Biolabs C2987I) before cleanup of the assembly reactions using AmpureXP magnetic beads (Beckman Coulter A63880) ...
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bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...
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bioRxiv - Immunology 2024Quote: ... the DNA was transformed into NEB® 5-alpha F’ I q chemically competent cells (NEB). Recombinants were selected on LB agar supplemented with 10 µg/ml of gentamicin ...
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bioRxiv - Molecular Biology 2024Quote: ... Cloning strains used were NEB Stable (lentiviral) and NEB 5-alpha (other plasmids) (New England Biolabs). For base editor cloning ...
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bioRxiv - Plant Biology 2024Quote: ... For molecular cloning and plasmid propagation Escherichia coli strain NEB® 5-alpha (New England Biolabs) and Stellar™ Competent Cells (Takara Bio Group ...
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bioRxiv - Biochemistry 2020Quote: ... pGFP2-N3 and pRluc-N1 for POR) after each was digested by the indicated pair of restriction enzymes (New England Biolabs, Inc.; Ipswich, MA). The multiple cloning sites of the vectors are upstream of the GFP or Rluc tag ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... FYVE-mApple-SmBiT was constructed by inserting SmBiT into pmApple-N1 at the C-terminus of mApple using the Q5 site directed mutagenesis kit (NEB M0492 and M0054) and then inserting the FYVE domain from FYVE-LgBiT at the N-terminus of mApple ...
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bioRxiv - Biochemistry 2022Quote: ... Finale expression constructs were subcloned in NEB 5- alpha competent E.coli cells (New England Biolabs, Ipswich, US) followed by Sanger sequencing (Microsynth ...
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bioRxiv - Immunology 2021Quote: ... The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The assembled plasmid was transformed into NEB 5-alpha (high efficiency) competent cells (New England Biolabs; C2987). After verifying the sequence ...
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bioRxiv - Bioengineering 2024Quote: ... Initial studies were done in an alternate NEB® 5-alpha strain (New England Biolabs, Catalog # C2987H) where trpA and trpB were replaced with a chloramphenicol resistance cassette ...
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bioRxiv - Molecular Biology 2021Quote: ... sequence of iRFP670 amplified from piRFP670-N1 vector by polymerase chain reaction (PCR) using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA) and primers encoding NcoI and XmaI digestion sites (Supplementary Table S1) ...