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Citations for New England Biolabs :
101 - 150 of 519 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... the reactions were subjected to RNase H cleavage by incubation with Thermostable RNase H (New England Biolabs) at a final concentration of 0.5 U/µL at 37°C for 1 h.
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bioRxiv - Microbiology 2020Quote: ... and 2 μL of endo-β-N-acetylglucosaminidase-H (Endo-H, EC 3.2.1.96) (New England Biolabs™) and incubated in the thermomixer at 37 °C for one h ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 h followed by 1 h incubation with 10 units of CIP Alkaline phosphatase (#M0290, NEB). The digested DNA samples were purified and added to a BrdU conjugate preadsorbed microplate prepared according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The relative abundance of oligomannose glycans was measured by digestion with Endoglycosidase H (Endo H; New England Biolabs). Digestion was performed overnight at 37 degrees ...
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bioRxiv - Microbiology 2022Quote: ... samples containing the same amount of protein (50 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The relative abundance of oligomannose glycans was measured by digestion with Endoglycosidase H (Endo H; New England Biolabs). Digested glycans were cleaned using a PVDF protein-binding membrane (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were reinjected at 75 μl/h and co-flowed with 150 μl/h RT mix (1.65x Maxima H-RT Buffer, 0.033 U/μl USER II (NEB), 15 U/μl Maxima H-RT (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... samples containing the same amount of protein (20 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 U RNase H (New England Biolabs) and incubated at 37°C for 30 min ...
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bioRxiv - Genomics 2020Quote: ... 100U/mL RNase H (New England BioLabs)) and incubated for 30min at 37°C with shaking ...
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bioRxiv - Cell Biology 2021Quote: ... 500 units endoglycosidase H (New-England Biolabs) was added to 40-µl aliquots of supernatant and pellet and incubated 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... We tested RNase H (New England Biolabs) on control unmodified and single modified RNA oligos and designed experimental chimeric oligos accordingly ...
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bioRxiv - Genomics 2020Quote: ... and 0.5 μL RNAse H (NEB #M0297S) to remove RNA ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μl RNase H (New England BioLabs), 1 μl 10X RNase H Reaction Buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and 1ul of Endo H HF (NEB). The reactions were incubated for 4h at and were terminated by adding 50μL 5x SDS sample buffer and boiled for 5 min before analyzing by immunoblotting ...
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bioRxiv - Synthetic Biology 2020Quote: ... RNase H (New England Biolabs Inc., M0297) and bovine serum albumin (New England Biolabs Inc. ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was treated with RNAse H (NEB) to minimize RNA contamination ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 µL RNase H (New England Biolabs), 644 µL buffer A and 2 µL DTT (1M) ...
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bioRxiv - Microbiology 2020Quote: ... and RNA digested with Rnase H (NEB). Paired-end sequencing was performed on an Illumina NextSeq 500 at the MIT BioMicroCenter ...
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bioRxiv - Molecular Biology 2021Quote: ... Two microliters of thermostable RNase H (NEB) was added along with 2 μL of 10× RNase H buffer at a final reaction volume of 20 μL ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Thermostable RNase H (New England Biolabs, USA) was added ...
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bioRxiv - Microbiology 2023Quote: ... plus 30 units of RNase H (NEB) or 15 µL nuclease-free water at 37°C for 3 hr ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was digested with RNase H (NEB) and RNase A/T1 (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... or Endo H (P0702, New England Biolabs) as previously described (41) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 U of Thermostable RNase H (NEB) was added to RNase A digestion ...
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bioRxiv - Biochemistry 2023Quote: ... 500 U Endo H (NEB, cat# P0702S) or distilled water ...
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bioRxiv - Genetics 2023Quote: ... 1 μl RNase H (New England BioLabs), 1 μl 10X RNase H Reaction Buffer ...
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bioRxiv - Biophysics 2023Quote: ... 1 μL RNase H (New England Biolabs) was added to the mixture to digest the RNA ...
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bioRxiv - Microbiology 2022Quote: ... 2μL of Thermostable RNase H (NEB M0523S), and 1μL of RNase H were added to each tube ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.0005 U/µL RNase H (NEB #M0297L), and 2.5 U/µL M-MuLV RT (NEB #M0253L ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.005 U/µL RNase H (NEB #M0297L) was added to one of the reactions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 5 U RNase H (NEB, M0297S) and incubated at 37°C for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... For Endo H (P0702L, New England Biolabs) treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl of 10x RNase H reaction buffer and 1 µl (5 units) of RNase H (New England Biolabs) were added to 8 µl of the reaction mixture and incubation was continued for another 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... samples were incubated for 1 h at 37°C with 1 µl Endo H in GlycoBuffer 3 (NEB P0702S) after denaturing in SDS sample buffer prior to running SDS-PAGE.
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bioRxiv - Biochemistry 2022Quote: ... The N-linked glycans and the CBP tag were cleaved by endoglycosidase H (Endo H, ∼0.2 mg per 10–15 mg purified protein, New England Biolabs) and HRV-3C protease (∼2 mg per 10–15 mg purified proteins ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins isolated from cell lysates were digested with Endoglycosidase H (endo H) enzyme (New England Biolabs, catalog #: P0703L) with G5 reaction buffer at 37°C according to manufacturer’s instruction and the published procedure 15 ...
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bioRxiv - Molecular Biology 2024Quote: ... an equal amount of DNA (8μg) was treated with 8□l of RNase H in 1x RNase H buffer (NEB) for 5 hours at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... RNAs were eluted by mixing the beads with 21µl RNase H Elution Buffer and 4 µl RNase H (New England Biolabs) and incubated at 37 °C for 30 min while shaking ...
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bioRxiv - Microbiology 2023Quote: ... Glycans were removed by incubating the immunoadhesins ON at room temperature (RT) with endoglycosidase H (endo-H, 0.8 U/mL, #P0702L, New England Biolabs) in 50 mM Na acetate-50 mM NaCl ...
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bioRxiv - Immunology 2024Quote: 0.5 mg of purified HK/68 or Sing/16 from HEK293S GnTI-cells was mixed with 20,000 units of Endoglycosidase H (Endo H; New England Biolabs) in non-denaturing reaction buffer to a final volume of 0.5ml and incubated at 37 °C for 5 h ...
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bioRxiv - Biochemistry 2020Quote: 20 μg of protein from SK-RC-39 cells were treated with N-glycosidase F (PNGase F) and Endoglycosidase H (Endo H) (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 h at 37°C in the absence or presence of RNase H or RNase A (40 U, NEB). Digested nucleic acids were cleaned with phenol-chloroform extraction and resuspended in nuclease-free water ...
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bioRxiv - Biochemistry 2021Quote: ... purified RNA was dissolved in 3 μl RNase H reaction mix (1x RNase H buffer [NEB], 40 pmol oligo(dT)12 ...
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bioRxiv - Biochemistry 2021Quote: 30 to 50 μg proteins were digested for 90 min at 37°C with endoglycosidase-H (Endo-H; P0702L) or endoglycosidase-F (Endo-F; P0705S) as recommended by the manufacturer (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... On-bead RNase H treatment control was performed for each reaction: washed beads were incubated with 300 µL 1X RNase H Buffer (NEB) plus 30 units of RNase H (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... or MnlI was at 37 °C for 2 h (EcoRI-HF) or 3 h (HhaI or MnlI) in rCutSmartTM Buffer (NEB). Nuclease S1 (Boehringer Mannheim ...
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bioRxiv - Microbiology 2024Quote: ... according to manufacturer’s instructions using 100 nM RNA-specific reverse transcription primer followed by RNase H digest with 5 µL RNase H (NEB) at 37 °C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... The relative abundance of oligomannose-type glycans was measured by digestion with Endoglycosidase H (per sample in 20 µl volume) (Endo H; New England Biolabs). A 6 µl aliquot of labelled glycans was combined with 1 µg endoH to a final volume of 20 µl ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl RNase H (5U final, NEB M0297L) was added directly to each reaction and incubation for 20 min at 37°C ...