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Citations for New England Biolabs :
451 - 500 of 519 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... at 37 °C for 1 h and ligated to a 3′ adaptor sequence using T4 RNA ligase 2 (truncated K227Q, New England Biolabs) at 22 °C for 3 h ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 20 pmol of the dephosporylated RNA were 5’-end-labeled (20 µCi of 32P-γATP) in a 20 µL reaction for 1 h at 37°C using 1 U polynucleotide kinase (NEB). Labeled RNA was purified on a G50-column (GE Healthcare ...
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bioRxiv - Genomics 2024Quote: ... RNA was the digested by adding 0.5 µL each of RNAse A and RNAse H (ThermoFisher R1253 and NEB M0297L) to each reaction and incubating for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1% NP-40 buffer as above and 20 µg of lysate or 50 µL of supernatants were subjected to Endo H (P0702S NEB) or PNGase F (P0709S NEB ...
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bioRxiv - Microbiology 2023Quote: ... 50 pmol of each RNA were dephosphorylated by incubation for 1 h at 37°C with 25 U of calf intestine alkaline phosphatase (NEB) in a final volume of 50 µL ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed three times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (anti-rabbit IgG HRP, 1:5000 dilution, 7074S, NEB) in TBS-T with 1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... we digested #126-pSC-T7A1reverse-parS with SpeI-HF and BamHI-HF 1 h at 37°C and heat-inactivated for 20 min at 80°C (New England Biolabs). Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega ...
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bioRxiv - Biophysics 2023Quote: ... The 38 kbp T7A1-parS + handle construct were subsequently digested for 1 h at 37°C and heat-inactivated for 20 min at 65°C using SrfI restriction enzyme (New England Biolabs).
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bioRxiv - Immunology 2023Quote: ... An aliquot of 15 μl containing trimer with fluorescently labelled glycans was treated with Endo H overnight at 37°C (New England Biolabs). Glycans were extracted with a polyvinylidene-fluoride protein-binding membrane (Merck Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... concisus for 2 h at 37°C in presence of 0.4 mM S-Adenosylmethionine (SAM, New England Biolabs, Ipswich, MA). After methylation ...
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bioRxiv - Cell Biology 2023Quote: ... Double-stranded cDNA was generated by second-strand synthesis via the nick translation method using a mix containing 2Lμl of RNAse H (NEB, #M0297S), 1Lμl of E ...
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bioRxiv - Microbiology 2023Quote: Lysates of HEp-2/ι1hUNGs cells mock infected or infected with wild-type HSV-1(F) at an MOI of 10 for 24 h were treated with alkaline phosphatase (CIP) (New England BioLabs) as described previously48.
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bioRxiv - Cell Biology 2023Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without 1 U Endo H (NEB, Frankfurt, Germany). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Cancer Biology 2023Quote: ... Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB). The lysates were boiled and subjected to SDS-PAGE and western transfer ...
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bioRxiv - Cell Biology 2023Quote: ... The unedited methylated DNA template present in the remaining sample was digested for 1 h at 37°C with DpnI restriction enzyme (New England Biolabs) in CutSmart® Buffer (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... followed by digestion to ribonucleotides by incubation for 2 h at 45 °C with 0.15 U of Nuclease P1 (NEB M0660S) in 10 mM ammonium acetate pH 5 ...
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bioRxiv - Microbiology 2023Quote: Lysates of U2OS cells infected with wild-type HSV-2 186 at an MOI of 3 for 24 h were treated with calf intestinal alkaline phosphatase (CIP) (New England BioLabs) as described previously60.
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bioRxiv - Microbiology 2023Quote: ... brucei BFs CN PIP5Pase exclusively expressing V5-tagged PIP5Pase WT or mutant D360A/N362A for 24 h using the magnetic mRNA isolation kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA products were purified by using a Zymo Oligo Clean and Concentrator kit and incubated for 20 min at 37°C in the presence or absence of RNase H or RNase A (New England Biolabs) at a low salt concentration followed by Protease K digestion (as above) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were then incubated for 6 h at 37 °C with or without 60 U of RNaseH1 (New England Biolabs) to ensure specificity of the following immunoprecipitation step ...
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bioRxiv - Biophysics 2024Quote: ... We digested the pBS-parS for 2 h at 37°C using NotI-HF or XhoI restriction enzymes (New England Biolabs) and heat-inactivated for 20 min at 80°C ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0) for 1 h at 37 °C and washes in CSTX buffer (1× CutSmart buffer (New England Biolabs (NEB) B60004) ...
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bioRxiv - Plant Biology 2024Quote: ... The reaction mixtures were incubated for 1 h at room temperature and mixed with 4 μL of 6X gel loading dye (New England Biolabs). Following electrophoresis in 1.2% agarose gel containing SYBR safe DNA gel stain (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... incubation at 30 °C for 1 h) and removed together with unused Linker 1 in a 30 min incubation with exonuclease RecJ (NEB). The reaction was stopped by heating at 65 °C for 20 min and products purified using a DyEx spin column (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting oligoduplex was ligated into a target plasmid vector predigested with BsmBI (55 °C for 2 h) using T4 DNA ligase (NEB). Cloning reactions were transformed into chemically competent NEB Turbo E ...
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bioRxiv - Genomics 2024Quote: ... CHART-enriched DNA was eluted twice in 200 μL of elution buffer supplemented with 5 U/μL RNase H (New England BioLabs) at room temperature for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... an aliquot of the dense DNA fraction of the second gradient was desalted and subsequently digested for 1 h at 37°C with single-strand-specific nuclease P1 and RNAseH (both New England Biolabs). Digestion-resistant DNA was purified by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Cell Biology 2024Quote: ... then 30 μl denatured lysate were treated with 2 μl Endo H or 1 μl PNGase F enzymes (New England Biolabs) in 40 μl reactions according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated with 100 μl CutSmart buffer containing 5 mM DTT and 1 mM dATP for 1 hour at room temperature before incubation for 2 h at 37°C in another 100 μl of the same buffer containing 1 μl Klenow exo-(NEB M0212S) and 1 μl T4 PNK (NEB M0201S) ...
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bioRxiv - Biochemistry 2020Quote: ... The dried glycoproteins were incubated with trypsin for 16 h at 37°C before releasing N-glycans using PNGase F (New England Biolabs, MA). Liberated N-glycans were separated from glycopeptides by C18 Sep Pak SPE cartridges (Waters Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... 1.5 mg of pNL003 plasmid (38) was linearized by incubation at 37 °C for 2-4 h in the presence of EcoRV (NEB R0195S). Transcription reactions (10 mL ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 µl Hind III (Fisher, FD0504), 3 µl EcoRI (Fisher, FD0274), and 3 µl Bam HI (Fisher, FD0054) in RNase H buffer (NEB, M0297) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 µg DNA was digested with 3 µl Hind III (Fisher, FD0504), 3 µl EcoRI (Fisher, FD0274), and 3 µl Bam HI (Fisher, FD0054) +/-5 µl RNase H (NEB, M0297) in RNase H buffer (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... 20µg of protein lysates and media samples were incubated with glycoprotein denaturation buffer at 95°C for 10 min and treated with 0.3µl Endo H (NEB; Catalog no #P0702) enzyme in glyco-buffer 3 for one hour at 37°C ...
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bioRxiv - Microbiology 2021Quote: A549 cells were infected with PR8 at a MOI of 5 and lysates were collected every two hours post infection (hpi) until 8 h RNA extraction was performed with Monarch total RNA Miniprep kit (New England Biolabs GmbH) according to manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 24-48 h with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of the extracted genomic DNA was digested for 4 h at 37☐ using 10 U MmeI (New England Biolabs). The DNA was then immediately dephosphorylated by treatment with 1 U calf intestine alkaline phosphatase (Invitrogen ...
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Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: ... 72 h post infection (hpi) and analysed by western blotting and pan anti-succinyllysine antibody (PTM-419, PTM Biolabs, Hangzhou, China), and non-inoculated cells served as control group ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL of the IEC fraction was concentrated for 4 h in vacuum to which 20 μL of the dried sample and 2 μL of 10xGlyco Buffer (New England Biolabs™) were added into 2 mL Eppendorf tube and incubated in the thermomixer at 99 °C for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µL of a 1000 U of DNase I stock was added to 500 µL of DNase I buffer on cells for 10 minutes or 2 µL of a 5000 U of RNase H stock (NEB M0297S) was added to 500 µL of RNase H buffer on cells for 5 minutes at 45 °C ...
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bioRxiv - Genomics 2021Quote: ... The sample was then allowed to cool to room temperature and incorporated in the Klenow enzyme mix (1x Maxima h-RT buffer, 1mM dNTP, 1U/μL of Klenow Exo-; NEB M0212L) was added to the single strand library ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 24-48 h with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 24-48 h with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin handle and cosmid-I95 DNA were both digested for 2 h at 37 °C with SpeI-HF (New England Biolabs, R3133L) and subsequently heat-inactivated for 20 min at 80 °C ...
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bioRxiv - Biophysics 2023Quote: ... These plasmids were digested with NotI-HF and XhoI for 2 h at 37°C (R3189, R0146, New England Biolabs, UK) and heat-inactivated for 20 min at 80°C.
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bioRxiv - Genetics 2023Quote: ... After 48 h cells were incubated for 30 min at 37 °C with SNAP-Oregon green (NEB, final concentration 4 µM). Cells were then incubated for 30 min at 37 °C in fresh media to wash off unbound substrate ...
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bioRxiv - Cell Biology 2022Quote: ... 6 μg of digested nucleic acids were treated overnight at 37 °C with or without 10 μl of RNase H (New England BioLabs, M029L) in 1x RNase H buffer and 1/10th of the samples was saved as input ...
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bioRxiv - Biochemistry 2023Quote: ... Purified PCR products and the destination vector pSIN-TRE3G-GFP-miRE-PGK-Neo were digested with XhoI/EcoRI 37°C for 1 h and the PCR product was ligated into the destination vector using T4 DNA ligase (NEB, # M0202L) overnight at 16 °C.
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bioRxiv - Microbiology 2023Quote: ... or treated with 0.1 mM carbonic anhydrase inhibitor – S4 for 24 h was subjected to semi-quantitative RT-PCR analyses using Q5 high-fidelity DNA polymerase (New England Biolabs Inc.) according to manufacturer’s protocol in a T100 Thermal cycler (BIO-RAD) ...
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bioRxiv - Neuroscience 2024Quote: ... and end repair was performed by incubating chromatin-bound beads for 1 h at room temperature in 1× T4 ligase buffer with ATP (NEB, B0202), 0.25 mM dNTPs (Thermo Fisher Scientific ...