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Citations for New England Biolabs :
1401 - 1450 of 10000+ citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The ligation mix (20 μl) was added to a 50 μl reaction containing 1X thermopol buffer (NEB), 200 μM each of dATP ...
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bioRxiv - Neuroscience 2023Quote: ... and amplified with primers containing indexes for sequencing using Q5 High-Fidelity 2xMaster mix (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... The integrity of MS2×128-containing lines was further confirmed by PCR using Q5 (New England Biolabs) and Terra (Takara ...
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bioRxiv - Biochemistry 2023Quote: ... Two annealed oligonucleotides containing the homology region were phosphorylated by T4 polynucleotide kinase (New England Biolabs, M201) and cloned into the vectors pX335 and pKN7 (see Appendix Table S1 ...
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bioRxiv - Genomics 2023Quote: ... The purified DNA was amplified using a reaction mixture containing 2X NEB PCR Master Mix (NEB M0541L), a universal forward primer i5 ...
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bioRxiv - Genetics 2023Quote: ... The PCR products containing the geneticin128-795-TtrpCand LB border were digested using AgeI (New England Biolabs) followed by a ligation reaction ...
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bioRxiv - Cell Biology 2023Quote: ... and iLID constructs were generated by PCR amplifying the parent constructs in pcDNA3.1 (GFP-Gγ3, GFP-Gγ9, and Venus-iLID) with overhangs containing expected nucleotide mutations and DpnI (NEB) digestion (to remove the parent construct ...
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bioRxiv - Biochemistry 2023Quote: BicD2 and NINL containing amino-terminal HaloTags were expressed in BL-21[DE3] cells (New England Biolabs) at an optical density at 600 nm of 0.4–0.6 with 0.1mM IPTG for 16h at 18°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 12·5 μL of Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs, M0494S), 1·1 μL of 10 μM SARS-Cov2-Midnight-1200 primer (either Pool 1 or Pool 2)(17 ...
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bioRxiv - Microbiology 2023Quote: ... pellets dried and resuspended in 100 µL RNase-free water containing 40 U murine RNase inhibitor (NEB). Following quantification by Nanodrop ...
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bioRxiv - Systems Biology 2023Quote: ... 22 μL of a mixture containing 10 mM of each of the four deoxynucleotide triphosphates (dNTPs) (NEB), 5.5 μL of TSO primer (100 μM) ...
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bioRxiv - Biophysics 2024Quote: ... 25μg of the plasmid containing the hsp70 promoter sequence was digested with HindIII restriction enzyme (NEB R3104) for 3 hours at 37C ...
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bioRxiv - Molecular Biology 2024Quote: ... Cole) containing an N-terminal 6×His-tag using NEBuilder HiFi DNA Assembly Master Mix (NEB E2621L). The LSD1 constructs were expressed in BL21-CodonPlus (DE3)-RIPL competent E ...
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bioRxiv - Genomics 2024Quote: ... Illumina TruSeq adapters were ligated to DNA fragments in a reaction containing 2X Quick ligation buffer (NEB), 0.25 μM of adapter and 2 μL of Quick ligase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... or 2 μL of Shortcut RNase III (0.02 L/μL; NEB) were added and incubated for 45 seconds ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 µl of 10x NEBuffer 3 (cat#B7003, New England Biolabs), and 13 µl of water were incubated in a thermocycler (cat#EP950040025 ...
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bioRxiv - Genomics 2020Quote: ... with 2 μl (5 U/μl) of Klenow fragment exo- (NEB) in a final volume of 55 μl by incubation at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in 2% bovine serum albumin (New England BioLabs) and were transferred immediately in ice to the genomics core laboratory.
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bioRxiv - Molecular Biology 2021Quote: ... 2 µg BSA dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 µl coating buffer (100 mM Tris-HCL pH 8 ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μL of 2-Log DNA Ladder (200 μg/mL; NEB) is mixed with 1 μL of Gel Loading Dye (6x ...
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bioRxiv - Genomics 2019Quote: ... 10 µL T4 RNA ligase 2 truncated (200 U/µL, NEB) was added ...
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bioRxiv - Systems Biology 2020Quote: ... and 2 μL NEBNext Second Strand Synthesis Enzyme Mix (E6111S, NEB) were added to the purified cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... 2 µL of T4 RNA ligase II truncated KQ (NEB M0373), 0.5 µL of SUPERaseIN ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with 2 U/μl of DpnII restriction nuclease (NEB) at 37 °C overnight ...
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bioRxiv - Genomics 2021Quote: ... the reaction was incubated with 2 μL of USER (NEB #5508) in 1X CutSmart Buffer at 37 °C for 15 min before final purification with Zymo Oligo Clean & Concentrator kit ...
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bioRxiv - Synthetic Biology 2021Quote: ... SARS-CoV-2 RNA was replaced with nuclease free water (NEB) for the assay without the target RNA ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 μl Q5 high-fidelity 2*master mix (New England Biolabs), 8.5 μl dH2O ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 1 mg/ml bovine serum albumin solution (NEB), 1 μL of hOGG1 (ProSpec TechnoGene Ltd. ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 units/µL T4 RNA Ligase 2 (truncated K227Q, NEB). The ligation reaction was incubated for 3 hours at 22 °C ...
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bioRxiv - Microbiology 2020Quote: ... after which 2 µL calf intestinal phosphatase (10,000 U/mL, NEB) was added to the digest and incubated for 1h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and 4 μl T4 RNA ligase 2 (NEB, catalog no. M0239) for 16 hours at 16°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 800U of truncated T4 RNA ligase 2 K227Q (New England Biolabs) in supplied PNK buffer with RNAsin (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... and RNA ligase 2 of bacteriophage T4 (New England BioLabs, Inc.). Reactions were incubated for one hour at 25 °C and ligated duplexes were purified by 1ml monoQ column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... An additional 2 μl RNase A (20 mg/ml, NEB #T3018) was added and followed by 5 min incubation at 56 °C to remove residual RNA in the sample ...
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bioRxiv - Plant Biology 2021Quote: ... in 1X NEB Buffer 2 (New England BioLabs; Ipswitch, MA, USA), and 0.1 mM dATP for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 1–2 mg of RNA was treated with DNAse I (NEB), x µL and 2 µL of this reaction was used to make cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... After the addition of 50ul of 10X NEBBuffer 2 (NEB, B7002) and 1ul of MboI restriction enzyme (NEB ...
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bioRxiv - Genomics 2020Quote: ... 2 μL 5x Q5 reaction buffer (New England Biolabs, Ipswich, MA), 0.2 μL 10 mM dNTPs (Kapa Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 µl NEB-Next High-Fidelity PCR Mix (NEB, cat # M0541S), 5 µl of SYBR Green (Invitrogen™ ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2 μl of the rare-cutter AseI (New England Biolabs, UK) was added and incubated at 30 °C overnight ...
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bioRxiv - Biophysics 2019Quote: ... The annealed strands were ligated with T4 RNA ligase 2 (NEB), in 20 μl reactions for 1h at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... 2 μl of 10X T4 RNA Ligase Buffer (New England Biolabs), 1 μl of 2 μM 5’-adenylated RNA linker ...
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bioRxiv - Genomics 2021Quote: ... 50 μL NEB Buffer 2 and 375 U MboI (NEB R0147M) were added and allowed to incubate for 2 hours at 37°C rotating ...
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bioRxiv - Biochemistry 2021Quote: ... 400 μM ATP (1x T4 RNA ligase 2 reaction buffer (NEB)) including 0.25 units/μL T4 RNA ligase 2 (Neb ...
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bioRxiv - Microbiology 2021Quote: ... Ligation was conducted using T4 double stranded RNA ligase 2 (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Oligos (2 μM each) were annealed in 1X CutSmart buffer (NEB) at 95°C for 5 min followed by cooling to room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mM MgCl2 and 2 μL (10 u) RNase H (NEB) in order to digest poly(A ...
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bioRxiv - Microbiology 2022Quote: ... 2 µg BSA and 3 units of T4 DNA polymerase (NEB) and incubated at 20 °C for 30 minutes ...
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bioRxiv - Genomics 2022Quote: ... and 2 μl of Klenow Fragment (3’→5’ exo-) (M0212L, NEB). The reactions were incubated at 37ºC for 30 minutes and then cleaned up with a Clean and Concentrate-25 column (Zymo) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 U/µl of T4 RNA ligase 2 truncated KQ (NEB), 10°C overnight) ...