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Citations for New England Biolabs :
1351 - 1400 of 10000+ citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: In vitro protein expression was carried out following manufacturer’s instructions (NEB, E6800s). Briefly ...
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bioRxiv - Immunology 2020Quote: ... 1x NEB buffer for Protein Metallophosphatase and 1mM MnCl2 (New England Biolabs) for 30 min at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... samples were diluted with 3x Blue Protein Loading Dye (New England Biolabs) and boiled for 5 minutes ...
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bioRxiv - Physiology 2021Quote: ... Proteins were then deglycosylated with PNGaseF (New England Biolabs, Ipswich, MA, USA) and digested with Lys-C (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was batch bound to 10 mL of chitin resin (NEB) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GFP-fusion proteins were purified from BL21(DE3) cells (NEB, # C2527H) using an N-terminal H6-tag ...
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bioRxiv - Biochemistry 2020Quote: Proteins have been expressed in Escherichia coli strain BL21 (DE3) (NEB # C2527). In brief ...
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bioRxiv - Immunology 2021Quote: ... The lysate and the precipitates were treated with Lambda Protein Phosphatase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 μg protein was mixed with 15 μL Glycoprotein Denaturing Buffer (NEB) in 150 μL total volume and incubated at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... while the other was treated overnight with Protein Deglycosylation Mix II (NEB) using non-denaturing reaction conditions according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... the MBP-fusion proteins were purified using amylose resin (New England Biolabs) and GST-fusions by glutathione-resin (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... incubated with λ protein phosphatase (40 units/μL) (P0753S, New England Biolabs) or recombinant TOPP4-GST/HIS for 1 h at 30□ ...
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bioRxiv - Plant Biology 2022Quote: ... MBP-fusion proteins were purified using Amylose Resin (New England Biolabs, E8021), following the manufacturer’s instruction with minor modification ...
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bioRxiv - Microbiology 2023Quote: ... and then associated with EnGen Spy Cas9 NLS protein (New England BioLabs) using the 4D-Nucleofector system (Lonza) ...
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bioRxiv - Microbiology 2024Quote: ... The molecular weight standards used were unstained broad range protein standards (NEB) and then the gel was run at 100 volts until the dye front was 1 cm from the bottom of the gel and then stained one hour in Coomassie blue stain (50% methanol ...
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bioRxiv - Molecular Biology 2024Quote: ... including a Blue Pre-stained Broad Range Protein Standard (New England Biolabs). Gels were either stained (with 0.5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µl protein A magnetic beads S1425S (New England Biolabs; Ipswich, MA) were washed 3 times in PBS and incubated overnight with 2-3 µg of antibody directed against CISD34 at 4°C with gentle mix ...
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bioRxiv - Cell Biology 2023Quote: ... and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified on glutathione-Sepharose beads (New England Biolabs), and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA was incubated at room temperature with Cas9 protein (NEB, Cat# M0646T). AB* embryos were injected at the one-cell stage with the sgRNA and Cas9 cocktail in a 1nL volume at 25pg sgRNA/nL ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were lysed and subsequently incubated with λ-protein phosphatase (NEB) for 1 h at 30°C according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were purified by NEBExpress Ni Spin Columns (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of 2X protein synthesis buffer (New England Biolabs, Beverly, MA), 0.25 μL of RNaseOUT RNAse inhibitor (Thermo-Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... This complex was co-injected with Cas9 protein (M0386M, NEB, Ipswich USA) into the yolk of 1-cell stage WT zebrafish embryos ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins with MBP-tag were loaded onto amylose resin (New England Biolabs) after elution from Ni-NTA ...
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bioRxiv - Genetics 2024Quote: ... and 400 units of Lambda protein phosphatase (λpp) (New England Biolabs, P0753S). The reactions were incubated at 30°C for 30 minutes and terminated by an equal volume of 2x Laemmli buffer.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were removed by treatment with proteinase K (20 units/ml) (NEB) and SDS (0.25% ...
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bioRxiv - Microbiology 2024Quote: ... MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using a pre-poured amylose column containing 4 mL amylose resin (New England Biolabs, E8021L) followed by size exclusion chromatography (protein buffer ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed in a 10 µl reaction volume containing 1X PCR Buffer (New England Biolabs), 10 µM dNTP and 0.05 U Taq DNA polymerase (New England Biolabs) ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated in 100 μl end-repair mix containing 1x T4 Ligase Buffer (NEB), 0.5 mM dNTP mix ...
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bioRxiv - Microbiology 2019Quote: ... with the reaction containing 5mM m7G(5′)ppp(5′)G RNA Cap Structure Analog (New England BioLabs). Resulting RNA was purified by lithium chloride precipitation and transfected into BHK-21 cells using Lipofectamine 3000 for viral production ...
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bioRxiv - Microbiology 2019Quote: ... with the reaction containing 5mM m7G(5′)ppp(5′)G RNA Cap Structure Analog (New England BioLabs). Resulting RNA was purified by lithium chloride precipitation ...
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bioRxiv - Genetics 2019Quote: ... The protospacer-containing plasmids were further modified by excising the Cas9 ORF with PstI (New England Biolabs). Each final plasmid contains the U6 promoter ...
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bioRxiv - Plant Biology 2021Quote: ... 2019) was replaced by the coding sequences containing the phosphosite mutations using Gibson Assembly (New England Biolabs). To create transformation vectors NPH3::GFP-NPH3-R18 and NPH3::GFP-NPH3-mR18 ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated in a final volume of 40μL containing 1X G5 reaction buffer (New England BioLabs) and incubated at 37°C for 4 hrs in the absence (mock ...
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bioRxiv - Molecular Biology 2022Quote: ... the modifications included employment of deoxy uridine containing primers and USER enzyme mix (New England Biolabs, UK) to create sticky ends that would facilitate the circularization of plasmid [9] ...
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bioRxiv - Microbiology 2022Quote: ... Parallel T7 reactions performed at the same time used 1.25 μg XbaI linearised pT7HCV(5’+)341N79wt and pT7HCV(5’+)341N79ko as templates in a 25μl final reaction volume containing 2.5μl 10 x T7 transcription buffer (NEB), 0.6μl RNaseOUT ...
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bioRxiv - Immunology 2020Quote: ... the pSS013-Cas9 vector containing a sgRNA against GRA24 was transfected along with NotI (New England Biolabs) linearized pLoxP-DHFR-mCherry-LoxP (Addgene Plasmid #70147) ...
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bioRxiv - Molecular Biology 2019Quote: ... the integrative plasmid pJRL2-pBMH1-yEGFP containing homologies to LEU2 was cut by AscI (New England Biolabs) and transformed ...
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bioRxiv - Biochemistry 2019Quote: ... Washed beads were incubated with 50 mM ammonium bicarbonate containing 1,000 units PNGase F (New England Biolabs) in a head-over-head rotator overnight at 37 °C.
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bioRxiv - Developmental Biology 2019Quote: ... E7.5) into 96 well PCR plates containing 2.5μl of methylase reaction buffer (1× M.CviPI Reaction buffer (NEB), 2U M.CviPI (NEB) ...
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bioRxiv - Immunology 2021Quote: ... the scFv containing region from isolated plasmids was amplified by PCR using Q5 high fidelity PCR (NEB). Illumina NGS samples were prepped and run using the Illumina MiSeq v3 reagent kit following manufacturer’s protocols pooling four library samples per lane ...
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bioRxiv - Genomics 2022Quote: ... was used in a 50 μl-reaction containing 5 μl of NEBuffer r3.1 (10X, NEB, cat# B7203), 26 μl of adapter-ligated DNA and NF water to 50 μl ...
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bioRxiv - Molecular Biology 2022Quote: ... containing hydrophilic streptavidin magnetic beads (equalling to 260 μl of the original bead suspension; New England Biolabs), pre-equilibrated with 1× Annealing Buffer ...
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bioRxiv - Microbiology 2022Quote: ... 10 min) and resuspended in 50 µl of 50 mM Tris-HCl containing 1 µg trypsin (NEB). After overnight incubation at 37 °C ...
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bioRxiv - Microbiology 2022Quote: pJJW101 derivatives containing one of the four NT ospA-targeting sgRNAs were cut with BglI (NEB, R0143), dephosphorylated with Quick-CIP (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... The ligation mix (20 μl) was added to a 50 μl reaction containing 1X thermopol buffer (NEB), 200 μM each of dATP ...
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bioRxiv - Biochemistry 2022Quote: ... before the unbound fraction containing cleaved Gβ1γ2 was dephosphorylated by treatment with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA isolated from lysed mouse aortic SMCs was digested with enzyme cocktail containing HindIII – XbaI – EcoRI (NEB). Following digestion ...