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Citations for New England Biolabs :
1051 - 1100 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 5′ adapter ligation with T4 RNA Ligase 1 (NEB, M0204L). The resulting RNA fragments were used for a reverse transcription reaction using Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... 0.1mM dNTPs and 1 U/μl USER enzyme (NEB, USA). 75μl of the mix was added and incubated for 3h at 37°C ...
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bioRxiv - Genomics 2019Quote: ... 2 μl of proteinase k (800 units ml-1, NEB) were added and reacted for 1 hour at 50°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... and dNTPs in 1× T4 DNA ligase reaction buffer (NEB), followed by dATP-addition with Klenow ...
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bioRxiv - Microbiology 2020Quote: ... 1× Phusion HF buffer (New England Biolabs GmbH, Schwalbach, Germany) with 1.5 mM MgCl2 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% DMSO and 1 U Phusion high fidelty polymerase (NEB) in 1x buffer provided by the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... and various amounts of the 1 kb ladder (NEB#N3232s) were run on 1% Agarose gel.
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bioRxiv - Cell Biology 2019Quote: ... 1% NP40 and PNGase-F (50 U/μl: NEB#P0704S).
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bioRxiv - Genomics 2019Quote: ... 34 µl of H2O and 1 µl of PstI (NEB) were added and restriction digestion was performed at 37°C for 4 h ...
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bioRxiv - Genomics 2021Quote: ... and 1 µl of PNK (NEB M0201, 10,000 units/ml) and incubated at 37 °C for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... were ligated using 1 μl Quick T4 DNA Ligase (NEB) and DNA fragments were amplified by PCR with NEXTFlex primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 µl T7 Endonuclease I (1 U/ul, M0302S, NEB) was added and incubated at 37°C for 1 hr ...
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bioRxiv - Genomics 2020Quote: ... 1 µl T4 RNA Ligase 2 truncated K227Q (200U; NEB)] was added ...
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bioRxiv - Genomics 2020Quote: ... 1 µl Calf Intestinal alkaline Phosphatase (10 U; CIP, NEB) or quick CIP (10 U ...
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bioRxiv - Cell Biology 2020Quote: ... 2700 units mL−1 murine RNase inhibitor (New England Biolabs), and 0.1 M Tris-HCl (pH 8.0)).81 Primers consisted of a poly-T sequence to trap mRNA molecules ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... and 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA). Labeling reactions were incubated for 2 h at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM sodium vanadate (New England Biolabs, Ipswich, MA, #0758s); 50 mM sodium fluoride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Reactions were treated with Exonuclease 1 (New England Biolabs, M0293S) for 45 minutes at 37℃ ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pulse-labeled with SNAPcell-505 (1 µM; NEB) for 20 min in media ...
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bioRxiv - Genomics 2021Quote: ... 0.5 μL of 1 mg/mLbovine serum albumin solution (NEB), 1.2 μL of 50 mM NAD+ (NEB) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μL DpnI enzyme (20 U, New England Biolabs #R0176L) was added to the 25 μL PCR mixture immediately after the final extension and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: 1 U (0.5 μl) RNaseIII (NEB Short Cut RNase III), was combined with 1 μl of 10X reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 U/μL exonuclease III (New England BioLabs®), while the other half of each cleanup was not treated ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 5 units/µl RNase H (NEB) were added and samples were incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 1 μl microccocal nuclease (New England Biolabs, Ipswich, MA, USA) and 2 μl benzonase (Merck-Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μl of T4 DNA ligase buffer (NEB, Ipswich, MA), 0.5μl of restriction enzyme (BsaI-HFv2 or Esp3I) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Lane M is DNA marker (NEB 1 Kb DNA Ladder), lane N shows the PCR product from negative control (ddH2O) ...
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bioRxiv - Developmental Biology 2020Quote: ... once with 1 mL NEBNext dA-Tailing reaction buffer (NEB) and then resuspended in 50 μL of with NEBNext dA-Tailing reaction buffer (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Triton-X 100 and 32 units Proteinase K(NEB) at 37C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: 1 ml of hydrophilic streptavidin magnetic beads (New England Biolabs) were washed 5 times with equal volume of Hybridization Buffer ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of human recombinant histone H4 (BioLabs, Cat# M2504S) was incubated with 50 μM n-butyryl- or isobutyryl-CoA and 0.2 μM of HAT1 at 30 °C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl of Vent Exo- DNA polymerase (New England Biolabs), and 0.5 μl of 0.1 nM oligonucleotide G (template oligonucleotide ...
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bioRxiv - Molecular Biology 2019Quote: ... 6.25 ul T4 RNA ligase 1 (10U/ul – NEB M0204). Next morning ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.4 μl DNAse 1 (2000 U/ml) (New England Biolabs), and 10 μl RNAse A (10 mg/ml ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1 U Q5 Hot Start High-Fidelity Polymerase (Q5, NEB), 3 % dimethylsulfoxide (DMSO ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 µM overexpressed T7 RNA polymerase (conversely New England Biolabs T7 RNA Polymerase M0251L can be used ...
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bioRxiv - Molecular Biology 2019Quote: 1 μg total RNA was treated with T4 PNK (NEB) in the presence of 10 mM ATP at 37 °C for 40 minutes and then cleaned up by ethanol precipitation ...
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bioRxiv - Genetics 2019Quote: ... pelleted and resuspended in 650 µl 1×DpnII buffer (NEB). Three 1.5ml tubes with 200 µl lysate each were treated in parallel with SDS (0.28% final concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... m6ACE RNA was digested with 1U XRN-1 (NEB M0338) in XRN-1 buffer [100mM LiCl ...
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bioRxiv - Genomics 2019Quote: ... and 1 µl of NEBuffer 2 (New England BioLabs #B7002S), then heating in a thermocycler at 95°C for 5 minutes ...
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bioRxiv - Genomics 2019Quote: ... we digested with 1 µl RNaseH (New England BioLabs #M0297S) at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of T7 Endonuclease I (NEB, 10 units/μl) was added and incubated at 37 °C for 15 min.
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bioRxiv - Molecular Biology 2020Quote: ... and afterwards with 1:100 pA-Dam (∼60 NEB units). After two washes with DigWash ...
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bioRxiv - Systems Biology 2019Quote: ... The PCR products were treated with Exonuclease 1 (NEB, M0293L) for 1 h at 37°C and purified by ethanol precipitation ...
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bioRxiv - Systems Biology 2019Quote: ... PCR products were treated with 40U Exonuclease 1 (NEB, M0293L) for 1 h at 37°C to remove free oligonucleotide and purified by ethanol precipitation/ or the DNA Clean and Concentrator 5 kit (Zymo Research ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µL of Hot Start High-Fidelity DNA polymerase (NEB)) using cycling conditions ...
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bioRxiv - Plant Biology 2020Quote: ... 1 µl of 10×CutSmart® Buffer (New England Biolabs) and Milli-Q water ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μl of PNGaseF (New England Biolabs, Ipswich, MA, USA) was added to this for an overnight incubation at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μl of Vent Exo-DNA polymerase (New England Biolabs), and 0.5 μl of plasmid DNA (Supplementary Table 3 ...