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Citations for New England Biolabs :
1001 - 1050 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL T4 DNA Ligase buffer (New England Biolabs), 1 μL T4 polynucleotide kinase (PNK ...
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bioRxiv - Microbiology 2023Quote: ... and 1 kb DNA ladders (New England Biolabs, N3232S). Amplicons were subsequently purified using the Genomic DNA Clean & Concentrator®-10 (D4011) ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 200 μl 1x NEBuffer 1 (NEB #B7001S) and 200 U NEB Exonuclease III (NEB #M0206S) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 U/mL murine RNase inhibitor (New England Biolabs), 60 nM linearized plasmid template ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% SDS and 10 U/ml Proteinase K (NEB), followed by an 18-hour incubation at 56 °C with vigorous shaking ...
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bioRxiv - Neuroscience 2023Quote: ... murine RNase inhibitor (1:1000, New England Biolabs, #M0314) and cycloheximide (0.5 mg/ml ...
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bioRxiv - Genetics 2023Quote: ... 1 ul of 5 mM 7-deaza-dGTP (NEB), 1 ul of 25 mM MgCl2 (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... according to the manufacturers’ instructions with DNase 1 (Biolabs) treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... or RNase If (1:100 diluted, New England Biolabs) in buffer 3 (100 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 U/μL murine RNAse Inhibitor (New England Biolabs), 62.5 nM fluorescent reporter (/5FAM/rUrUrUrUrUrU/IABkFQ/ ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with murine RNAse Inhibitor (1 U/μL, NEB), fluorescent reporter (500 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... and the MCS-1 using MluI-HF (NEB; # R3198S) and SalI-HF (NEB ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl of murine RNase inhibitor (New England Biolabs), 1 μl of 10 mM dNTP mix (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 U/μl of DNase I (RNase free) (NEB) was added to each reaction followed by 30 min incubation at 37°C for the elimination of the DNA templates ...
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bioRxiv - Neuroscience 2024Quote: ... 1% (v/v) BSA (20 mg/ml, NEB, B9000S) + 5μL of hash oligo (10 uM ...
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bioRxiv - Molecular Biology 2024Quote: ... Gaussia princeps luciferase (New England Biolabs, #E8023, 1:5,000), Histone H3 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by overnight treatment with apyrase (1-unit, NEB) on ice ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μL of 20 mg/mL Proteinase K (NEB), and 1 μL of 10 mg/mL RNase A (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μL recombinant shrimp alkaline phosphatase (NEB cat#M0371S) was added to the reaction and incubated at 37°C for 1hr ...
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bioRxiv - Physiology 2024Quote: ... using T4 RNA ligase 1 (M0204S; New England Biolabs) at 16°C overnight ...
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bioRxiv - Genomics 2024Quote: ... 1 µL of MseI (New England Biolabs, Cat #R0525S), 1 µL of both the reference and target primer assays ...
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bioRxiv - Cell Biology 2024Quote: ... and complexed with Cas9 (1.5 µL 1 µM, NEB). CRISPR/Cas9 complexes and 100 pmol annealed repair template were electroporated (Neon transfection system ...
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bioRxiv - Immunology 2024Quote: ... and shrimp alkaline phosphatase (rSAP, 1 U, NEB, M0371S) in 50 μL RNase-free water at 37 °C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 62.5 units per 1 million cells MNase (NEB; M0247S) was added to resuspended pellets ...
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bioRxiv - Synthetic Biology 2024Quote: ... using 1 x Hotstart Taq master mix (NEB, M0496S), attB_MiSeq forward primer ...
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bioRxiv - Systems Biology 2022Quote: ... 5’-AAAC(N)19-20-3’) by combining 1 μl of each 100 μM oligonucleotide with 1 μl of 10× T4 ligation buffer (NEB cat. no. B0202S), 6.5 μl of water ...
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bioRxiv - Genomics 2020Quote: ... 50-100 fmol of PCR products were diluted in 9 µl then mixed with 1 µl of Rapid 1D Adapter and 1 µl of Ligase T4 Blunt (New England Biolabs, Ipswich, MA, USA). Pre-sequencing mix was incubated 10 min at 25°C and left on ice until ready to load ...
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bioRxiv - Microbiology 2022Quote: ... We then used 1:3 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). After the HiFi assembly ...
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bioRxiv - Immunology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). The Hifi assembly products were Ampure bead purified and eluted into 20 µL of H2O ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Biochemistry 2023Quote: ... In vitro methylation experiments were performed for 10 min (DNA) or 1 h (RNA) at 37 °C in 8 μL reaction mixtures containing 1× CutSmart buffer (New England Biolabs Inc., Massachusetts, USA) with 20 nM DNA or 50 nM RNA with 1 unit RNase Inhibitor (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2, truncated, NEB, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 µM of the 6-kb fragment was incubated with 1 unit/µL terminal deoxynucleotidyl transferase (TdT, New England Biolabs, MA, USA, #M0315S) and 0.5 mM deoxyadenosine triphosphate (dATP ...
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 µg of empty vector and 1 µg of purified amplicon were digested overnight with 1 µl of SphI-HF® (New England Biolabs, catalog no. R3182) in a total volume of 50 µl ...
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bioRxiv - Biophysics 2019Quote: ... the imaging chamber was incubated in blocking buffer (10 mM Tris HCl pH 7.5, 50 mM NaCl, 1 mg/mL BSA [NEB], 1 mg/mL tRNA [Ambion]) for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... and 15ul BseYI-digested product were run side-by-side on gel (1% agarose gel in TAE buffer, 1 kb ladder (NEB, Cat. No. / ID: N3232L), most gels run at 115V for 40 minutes) ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...
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bioRxiv - Genetics 2021Quote: ... T7 endonuclease 1 assay was performed following manufacturer’s protocol (NEB).
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bioRxiv - Developmental Biology 2021Quote: ... and mounted in 1% low melting point agarose (LMP) (Biolabs) within a 1mm diameter glass capillary ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (10 U/μl) of T4 polynucleotide kinase (NEB) and 1 μl of 10 mM dNTPs in a final volume of 50 μl at 24°C for 30 min ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (3 U/μl) of T4 DNA polymerase (NEB), 1 μl (10 U/μl ...
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bioRxiv - Physiology 2022Quote: ... anti-MBP (1:10,000, catalog no. E8032S, New England Biolabs); anti-CCNA2 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Excess adapter was digested by adding 1 µl RecJf (NEB) and 1 µl 5’deadenylase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... both proteins were deglycosylated by PNGase F (NEB, 1:50) overnight at 37°C in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-GFP (1:1000; TP401, Torrey Pine Biolabs, RRID:AB_10013661), mouse anti-GFP (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... and 1 unit of poly(A) polymerase (New England Biolabs) and was incubated at 42°C for 1 hour followed by enzyme inactivation at 95°C for 5 minutes ...