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Citations for New England Biolabs :
1051 - 1100 of 1299 citations for Mouse Anti Hepatitis C Virus NS4b Antibody 1858 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The appropriate N-or C-terminal poly-histidine or strep-tags were engineered onto BchB using Q5 site-directed mutagenesis (New England Biolabs, Ipswich, MA). The primers used to generate the corresponding sequence change are listed (Supplemental Table 1) ...
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bioRxiv - Cell Biology 2021Quote: ... grown to an ideal OD and induced with IPTG as per manufacturer’s instructions at 25°C (Sigma Aldrich for pGEX and New England Biolabs for pMal). Cells were resuspended in lysis buffer [2.5 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... The heat-denatured samples were incubated at 37 °C for 1 h with 500 units of Endo H (endoglycosidase H; New England Biolabs, Ipswich, MA) in a final volume of 20 µl containing 1 × GlycoBuffer ...
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bioRxiv - Genetics 2021Quote: PCR products (150 – 200 ng) were digested for 30 min at 37°C in 20 ul 1X restriction enzyme buffer (NEB buffer v3.1) containing 500 ng Cas9 (Alt-R® S.p ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then 3’dephosphorylated by denaturing at 65°C for 5 min and incubating with T4 PNK (NEB, catalog no. M0201S) in a 10 μL reaction (7 μL precipitated RNA ...
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bioRxiv - Microbiology 2021Quote: Vector pFLD was digested with PmlI at 37°C for 2 h and dephosphorylated with 5 U Antarctic phosphatase at 37 °C for 30 min as recommended by the manufacturer (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Genomics 2021Quote: ... The Hi-C library for Illumina sequencing was prepped using the NEBNext® Ultra™ II DNA library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Adapter ligation (AMX) was performed at RT (20 °C) for 20 min using NEB Quick T4 DNA ligase (New England Biolabs, MA, USA). The reaction mixture was purified using 0.6X AmPure beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... deglycosylation treatment (Dglyco) consisted of sample incubation for 3 hours at 37°C with 20 nL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per μL of sample ...
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bioRxiv - Genomics 2022Quote: ... samples were gently removed from the chamber and digested overnight at 37°C in 8 U mL-1 proteinase K (NEB, cat# P8107) in digestion buffer (1× TAE buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Adapter ligation was performed at 20°C for 10 min, with Adapter Mix (AMX, ONT) and Blunt/TA Ligase Master Mix (New England BioLabs, cat# M0367). After a final 1X clean-up and washing of the beads with Adapter Binding Buffer (ABB ...
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bioRxiv - Synthetic Biology 2022Quote: ... The Hi-C library for Illumina sequencing was prepped by NEBNext Ultra II DNA library Prep Kit for Illumina (NEB Cat# E7645S) according to the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 16 °C for 4 h in the presence of 1.15x of T4 ligation buffer (New England Biolabs, catalog no. B0202S) and 100U T4 ligase ...
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bioRxiv - Microbiology 2023Quote: ... and amplified with the primers (5’-CAA GAC TAG TGG AAG CGG AGC TAC TAA CTT CAG CCT GCT GAA GCA GGC TGG CGA CGT GGA GGA and 5’-NNN NAC GCG TCT AGC CTT CCC AGA CGT ACC C) using high-fidelity Phusion polymerase (NEB, Cat# M0530S). The PCR fragment was digested with BmtI and MluI ...
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bioRxiv - Microbiology 2023Quote: ... the Strep-tag® II sequence was inserted at the C-terminus via Phusion® High-Fidelity DNA Polymerase mutagenesis PCR (New England Biolabs) using the primers pYS14_GA_F/ 5’ phosphorylated flaakstrp14R to amplify the whole plasmid ...
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bioRxiv - Microbiology 2023Quote: ... A total of 3 μg of RNA was denatured at 65°C for 10 min with equal volume of RNA loading dye (New England Biolabs, MA, USA). RNA samples ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Addition of polyA-tail: Incubate at 72°C for minutes in 12 µl total volume including 10x Standard Taq Reaction Buffer (NEB Cat. #B9014S), dATP (NEB Cat ...
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bioRxiv - Genetics 2023Quote: ... Five microgram samples were individually digested overnight at 37°C with different methylation-sensitive restriction enzymes (HaeIII, AvaII, HpaII, Sau3AI and PspGI) (New England Biolabs; Ipswitch, MA). A control digest was also prepared using McrBC for 2 hours at 37°C (Xie et al. ...
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bioRxiv - Microbiology 2023Quote: ... DNA was ligated for 4 h in a water bath at 16 °C (30 Weiss Units of T4 DNA Ligase, M0202 New England BioLabs® Inc). 300 μg of Proteinase K was used to reverse the cross-linking overnight at 65°C ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Lentiviral related PLD3/4 plasmids were cloned into pBOBI vector that has a C-terminal FLAG tag after digestion by restriction enzymes BamHI and XhoI (NEB R3136S, R0146S). All these plasmids were prepared with endotoxin-free kit (Takara 740426 ...
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bioRxiv - Genetics 2023Quote: ... 5’ and 3’ homology arm flanking the C-terminal insertion site was amplified from N2 genomic DNA using the Q5® High-Fidelity DNA Polymerase (NEB, M0491L), followed by HiFi DNA assembly (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37° C for 2 hrs from 1 µg purified dsDNAs using a T7 high yield RNA synthesis kit (NEB, Cat # E2040S) in a total volume of 20 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1pmol of each fragment were then assembled for 1h at 50°C using NEBuilder HiFi DNA Assembly master mix from the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, ref E5520S). Reaction products were amplified in provided bacteria and purified using QIAprep Spin Miniprep Kit.
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bioRxiv - Genomics 2024Quote: ... Small RNA sequencing libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following the manufacturer’s recommendations with the 3’ ligation step changed to 16°C for 18 hours to improve capture of methylated small RNAs (New England Biolabs, cat# E7300S). Small RNA PCR amplicons were size selected on 10% polyacrylamide non-denaturing gels ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNA was then treated with DNase for 10 min at 37◦C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The mouse C10 G54R mutation removes a cut site of the restriction endonuclease BanII (NEB, cat# R0119S), introducing a restriction fragment length polymorphism ...
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bioRxiv - Cell Biology 2023Quote: ... DNA isolated from lysed mouse aortic SMCs was digested with enzyme cocktail containing HindIII – XbaI – EcoRI (NEB). Following digestion ...
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bioRxiv - Cancer Biology 2023Quote: Mouse NIK coding sequence in the pENTR shuttle plasmid was mutated using Phusion high fidelity polymerase (NEB) and oligonucleotides designed to carry the desired mutations (indicated by lowercase letters) ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Bioengineering 2024Quote: ... Amplicons from mouse embryo lysates were generated using Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 25 μL reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-m6A (E1610S, NEB; 202003, Synaptic Systems), Flag tag Monoclonal Antibody (66008-2-Ig ...
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bioRxiv - Microbiology 2021Quote: ... and anti-Kglu (PTM-1152, PTM Biolabs), followed by quantification using Quantity One (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... anti-glutarylated lysine (PTM Biolabs, 1:1000), anti-pAMPK (Cell Signaling Technology #2532S 1:1000) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-succinylated lysine (PTM Biolabs 1:1000), anti-glutarylated lysine (PTM Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... anti-malonylated lysine (PTM Biolabs, 1:1000), anti-succinylated lysine (PTM Biolabs 1:1000) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-SNAP-tag (New England Biolabs, #P9310S) and anti-Cyclin D1 (BD Bioscience ...
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bioRxiv - Microbiology 2022Quote: ... and the Anti-Reverse Cap Analog (NEB) was used at 2.8 mM ...
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bioRxiv - Cell Biology 2022Quote: ... anti-ubiquityl-PCNA (Lys164) (NE Biolabs 13439S) (1:1000) ...
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bioRxiv - Systems Biology 2022Quote: ... anti-SNAP (1:1000, rabbit, NEB, P9310S); anti-MRPS18B ...
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bioRxiv - Genetics 2020Quote: ... anti-MYC-tag (Cell Signaling Technology/NEB) at 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... pan anti-glutarylysine (PTM-1151, PTM Biolabs) and pan anti-acetyllysine (PTM-105 ...
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bioRxiv - Synthetic Biology 2023Quote: ... rabbit anti-Sox-2 (New England Biolabs), and rabbit anti-NANOG (New England Biolabs) ...
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bioRxiv - Immunology 2022Quote: ... anti-Histone H3 (4499, New England Biolabs) and anti-mouse IgG-HRP (Santa Cruz Biotechnology) ...
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bioRxiv - Immunology 2022Quote: ... anti-α-tubulin (2144, New England Biolabs), anti-Histone H3 (4499 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and rabbit anti-NANOG (New England Biolabs). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... anti-H3K9la (PTM BIOLABS, PTM1419RM, 1:1000), anti-H3K14la (PTM BIOLABS ...
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bioRxiv - Cell Biology 2023Quote: ... anti-H3K14la (PTM BIOLABS, PTM1414, 1:1000), anti-H3K18la (PTM BIOLABS ...
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bioRxiv - Cell Biology 2023Quote: ... anti-H3K18la (PTM BIOLABS, PTM1406, 1:1000), and anti-H4K12la (PTM BIOLABS ...