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Citations for New England Biolabs :
801 - 850 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... coli Protein Synthesis System (NEB) [48] at 2 x the reaction scale ...
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Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organismbioRxiv - Molecular Biology 2024Quote: ... coli tRNA (New England Biolabs), 15% formamide (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... coli cells (New England Biolabs) grown to OD600 ≈ 0.5 and induced with 0.5 mM IPTG for 3 hours at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Coli UDG (New England Biolabs Inc. ...
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bioRxiv - Genetics 2024Quote: ... coli (NEB cat no. C2992H) and resulting bacterial colonies used to inoculate 50 mL of LB and grown at 37°C for 6 h at 250 rpm in an orbital shaker ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain (New England Biolabs) was used for cloning and plasmid propagation.
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bioRxiv - Microbiology 2024Quote: ... coli NiCo21(DE3) (NEB BioLabs) was used to express recombinant proteins ...
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bioRxiv - Biochemistry 2024Quote: ... coli cells (New England Biolabs) was inoculated into 1L of LB medium and induced with 0.1mM IPTG at 18C for 18hours ...
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bioRxiv - Biophysics 2024Quote: ... coli NiCo21(DE3) strain (NEB). Cells were grown at 37°C supplemented with 2XYT medium (Melford ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Poly(A) polymerase (NEB), and m7Gppp5′N cap was added using Vaccinia Capping System (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... coli (New England Biolabs, Inc.) in 300 ml of Luria-Bertani broth supplemented with Kanamycin (50 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... via recombinant neuraminidase (NEB P0720L) as described by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... or Amylose resin (MBP-tagged fragments, New England Biolabs) following the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2022Quote: ... AAK1(S-676D/E/A) and UVRAG (T-518D/E/A) using Q5-site directed mutagenesis Kit (NEB). Manufacturer’s instructions for mutagenic primer design were followed ...
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bioRxiv - Biophysics 2022Quote: ... 2018) and a C-terminal 6x His tag and cloned into the pET21B vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Biophysics 2022Quote: ... The DNA fragment was amplified by PCR by inserting a C-terminal 6x His tag and cloned into the pETDUET-1 vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Plant Biology 2020Quote: ... coli ER2566 cells (New England Biolabs). Expression of CBC-Qprot as a 15N-labeled protein and purification via Co-NTA affinity chromatography and electroelution was performed as described previously for the photosynthesis QconCAT protein (PS-Qprot ...
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bioRxiv - Cancer Biology 2021Quote: ... coli poly(A) polymerase (NEB Biolabs), and capped and 2’-O-methylated with Vaccinia Capping System (NEB Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells (New England Biolabs Inc.) and selected using antibiotics (Ampicillin).
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bioRxiv - Microbiology 2021Quote: ... coli DNA polymerase (New England Biolabs), and 0.25µL of RNaseH (New England Biolabs) ...
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bioRxiv - Developmental Biology 2020Quote: ... coli DNA ligase (NEB, #M0205 L), 5 μl of E ...
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bioRxiv - Developmental Biology 2020Quote: ... coli DNA Polymerase (NEB, #M0209 L), 1 μl of dNTP (0 .2 mM) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli (NEB Inc.; catalog no. C2527) and growing it in LB media supplemented with 100 μg/ml of selective antibiotics (kanamycin for His-tagged E2-WT and E2-S23D ...
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bioRxiv - Cell Biology 2019Quote: ... coli cells (New England Biolabs, C2987I) were used for all cloning steps ...
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bioRxiv - Microbiology 2019Quote: ... coli expression vector (New England Biolabs). The resulting recombinant variants were expressed as maltose-binding protein (MBP ...
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bioRxiv - Bioengineering 2019Quote: ... coli strain NEB5α (New England Biolabs) for plasmid recircularization and production ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli T7 Express (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: ... coli exonuclease I (New England Biolabs) was used to digest all molecules with free ends ...
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bioRxiv - Molecular Biology 2020Quote: ... coli Poly(A) Polymerase (NEB-M0276S). Poly(A)-tailed RNAs were purified using RNAClean XP beads ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain (C2527I, New England BioLabs). Cultures were grown in 250mL 4xYT-carbenicillin in a 1-liter baffled flask at 37 °C and 300rpm until the OD600 reached between 0.7-0.9 ...
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bioRxiv - Genetics 2020Quote: ... coli (New England Biolabs, Beverly, MA) and single colonies picked for culturing ...
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bioRxiv - Microbiology 2021Quote: ... coli T7 expression strain (NEB 3016). Around 1920 E ...
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bioRxiv - Microbiology 2021Quote: ... coli strain ER2566 (New England Biolabs) containing a pET-24b(+ ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (New England Biolabs, NEB) and confirmed by PCR using relQ gene specific primers (relQ_a_F & relQ_a_R) ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (New England Biolabs, NEB) and confirmed by PCR using relQ gene specific primers (relQ_a_F & relQ_a_R) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli (NEB 5-alpha competent cells), isolated using the Monarch Plasmid preparation kit (NEB ...
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bioRxiv - Biophysics 2020Quote: ... coli BL21 (DE3) (New England Biolabs). Bacterial cultures were grown overnight at 37 °C in LB or M9 minimal medium and induced at OD600 of ca ...
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bioRxiv - Bioengineering 2020Quote: ... coli (Iq, c3013, New England Biolabs) and SHuffle T7 Express lysY Competent E ...
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bioRxiv - Bioengineering 2020Quote: ... coli (SH, c3030, New England Biolabs) following the manufacturer’s heat-shock protocol and using the ampicillin selection marker ...
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bioRxiv - Bioengineering 2021Quote: ... coli (New England Biolabs, Ipswich, MA) and isolated with the Zymopure II Midi Prep kit following the manufacturer’s protocol (Genesee Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... coli RNA polymerase reaction buffer (NEB) (40 mM Tris HCl ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 (DE3) (New England Biolabs). In an attempt to improve transglycosylation ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... coli DH10-beta (New England Biolabs) was transformed with Gibson assembly product ...
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bioRxiv - Neuroscience 2021Quote: ... coli (New England Biolabs, cat. #C25271) were transformed with pGEX-4T1-cCPEm and grown to OD600 ~ 0.6 ...
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bioRxiv - Microbiology 2021Quote: ... coli strain NEB10β (New England Biolabs), and sequences of all inserts were verified by sequencing ...
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bioRxiv - Microbiology 2021Quote: ... coli strain NEB10β (New England Biolabs), and sequences of all inserts were verified by sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... coli Poly(A) Polymerase (NEB M0276S). The poly(A ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21(DE3) competent cells (NEB). A single colony was picked and inoculated into 5 mL of LB+Amp medium in a 15mL falcon tube ...
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bioRxiv - Microbiology 2020Quote: ... coli NEB 10 (New England Biolabs) was grown in LB medium and E ...
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bioRxiv - Microbiology 2021Quote: ... coli were transformed (New England Biolabs). Positive colonies were screened by restriction digest ...