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Citations for New England Biolabs :
1001 - 1050 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 1 μL BST DNA Polymerase FL (NEB, 5 U/µL), 2μL TAQ Ligase (NEB ...
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bioRxiv - Immunology 2024Quote: ... and 5 μL of Proteinase K (NEB catalog no. P8107S). Tubes were incubated for 15 min at 37 °C with shaking at 600 rpm ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl inorganic pyrophosphate (New England Biolabs product number M2403), and ultrapure water (as needed ...
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bioRxiv - Genomics 2024Quote: ... and dTTP with 5 U/µL DNA polymerase (NEB # M0210), the DNA was ligated by T4 DNA ligase (NEB # M0202 ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of Klenow DNA Polymerase (5 U/μL, NEB) and 2 μL of T4 PNK (10 U/μL ...
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bioRxiv - Genomics 2024Quote: ... and 5 µl DNA Polymerase I (Large Klenow Fragment) (NEB). The reaction was incubated for 60 min at 37 °C and subsequently purified and eluted in 13 µl ddH2O using a MinElute kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: Chemically competent NEB® 5-alpha F’Iq cells (NEB; C2992H) were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective Luria-Bertani (LB ...
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bioRxiv - Genomics 2024Quote: ... and 3 μl of 5′-deadenylase (NEB, Catalog no. M0331S) to the 60 μl end-prepped product ...
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bioRxiv - Bioengineering 2024Quote: ... 5’-end labeling was performed using T4 Polynucleotide Kinase (NEB) and [gamma-32P] ATP (PerkinElmer) ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of T4 DNA ligase (NEB, 400000 U/mL), and 10 μL of 10X T4 DNA ligase buffer (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μl H2O and 1 μl PNGase F (NEB, P0704S) were then added to the lysate ...
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bioRxiv - Bioengineering 2024Quote: ... then 5 μL of 6X Purple Loading Dye (NEB, B7024S) were added and run on a GelRed-stained 1% (w/v ...
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bioRxiv - Genomics 2020Quote: ... A quick Ligation Kit (New England Biolabs Inc., MA, CA#M200l) was used for ligating the insert and vector ...
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bioRxiv - Genomics 2023Quote: ... and 200 ng/µl Cas 9 protein (New England BioLabs, M0386T). Injected individuals were screened (see below genotyping procedure ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 15,000 x g for 30 min at 4°C and the clarified extract was loaded onto a chitin resin (NEB) column pre-equilibrated with column buffer for purification at RT ...
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bioRxiv - Microbiology 2021Quote: ... The amplicon was purified and digested using 30 units of SacI-HF and SalI-HF (NEB, #R3156S and R3138S respectively) at 37 °C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Labelling of total histones was performed by a 30 min-pulse with 2 µM of the SNAP-cell SiR-647 reagent (New England Biolabs) followed by 30 min wash in fresh medium.
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bioRxiv - Molecular Biology 2021Quote: ... the samples were treated with Klenow exo- at 37 °C for 30 min to fill the ends of adaptors and then trimmed with T4 DNA polymerase (NEB) at 12 °C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: In vitro cleavage assay with the purified AsCas12a and SpCas9 nucleases was carried out in a volume of 30 µL in 1× NEB2.1 buffer (New England Biolabs Inc.). The standard reaction containing 10 nM of PCR-obtained DNA template ...
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bioRxiv - Cancer Biology 2021Quote: ... the ligation of the custom sequence adapters was done in solution by adding 4 μl adaptors (30 μM) and 1600 units T4 DNA ligase (NEB). The ligation was carried at for 2 hours at room temperature on a rotating wheel in 1x ligation buffer (NEB) ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was collected after a spin at 16000 g for 30 min and incubated with GFP-Trap (Chromtek) or MBP magnetic beads (NEB) for 3 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The RNA fragments were ligated with 3’-barcoded adaptors at 22 °C for 1 h and 30 min using T4 RNA Ligase (NEB). Barcoded RNA samples were pooled together ...
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bioRxiv - Microbiology 2020Quote: ... RNA was fragmented via ultrasound (4 pulses a 30 sec, 4 °C) and subsequently treated with T4 Polynucleotide Kinase (NEB). Half of the samples were then treated with terminator exonuclease (TEX ...
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bioRxiv - Biochemistry 2022Quote: 30 uL of tagged Fluc-WT proteins were incubated with Factor Xa (NEW ENGLAND BioLabs, final concentration 67 ug/mL) for 2 hours or overnight on ice.
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bioRxiv - Genomics 2022Quote: ... Poly-A mRNA was eluted in 36 μl of nuclease free water and 12 μl was reverse transcribed in a 30 μl reaction using the Template Switching RT Enzyme Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 40 μl of filtered double-distilled water and 20 or 30 μl of Blue Protein Loading Dye (New England Biolabs) were added to the beads or the inputs ...
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bioRxiv - Molecular Biology 2022Quote: ... and a volume of cDNA corresponding to 5 ng of the input RNA was subjected to 30 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
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bioRxiv - Synthetic Biology 2022Quote: ... 50 ng of genomic DNA from genome-targeting experiments were PCR amplified with 30-cycles using Q5 High-fidelity DNA Polymerase (NEB) and primers which bind just outside of TS2 or just inside of LE ...
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bioRxiv - Developmental Biology 2022Quote: ... coli and by performing heat shock for 30 seconds at 42°C followed by recovery in SOC outgrowth medium (NEB) for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... digestion at 37°C for 30 minutes and then purified with the Monarch RNA Cleanup Kit (T2050, New England Biolabs) for long RNA (1020 nucleotides to 4187 nucleotides ...
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bioRxiv - Molecular Biology 2020Quote: ... End-repair reaction was cleaned using 1.8X Agencourt AMPure XP beads and eluted in 15 µl of EB that was used for A-tailing reaction in 30 µl of NEBNext dA-Tailing reaction buffer (NEB) with 7.5 units of Klenow fragment exo- (NEB ...
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bioRxiv - Immunology 2021Quote: Similarly for ChIP seq 30 μl ChIP-DNA was processed for library preparation according to ChIP-seq library preparation protocol (NEB) [25] ...
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bioRxiv - Biochemistry 2020Quote: ... Hartmann Analytic) for 30 min at 37°C in 50 µl volumes with 20 units of T4 PNK (New England Biolabs) in the provided reaction buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... the concentration of eluted MBP-mintbody was adjusted to 1 mg/ml and digested with 30 μg/ml Factor Xa (New England Biolabs) for 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... One-way assays were carried out as above except for the exclusion of Gyrase and SmaI was substituted for 30 units Ncol-HF (NEB) or 30 units SacI-HF (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μl RT mastermix (2x reaction buffer, 20 mM DTT, 4U murine RNase inhibitor [NEB], 30 U ProtoScript II Reverse Transcriptase [NEB]) were added ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Synthetic Biology 2021Quote: ... [35] obtained from Benenson lab at ETH Zurich was digested with 30 U of SalI-HF (New England Biolabs (NEB), R3138S ...
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bioRxiv - Microbiology 2021Quote: ... 200 ng ΔVC1807::ErmR transforming DNA was added and reactions were incubated at 30 °C for 10 minutes before the addition of 10 units of DNAse I (NEB) to prevent additional DNA uptake ...
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bioRxiv - Microbiology 2020Quote: ... The pKAS32 vector was restriction digested using SacI and XbaI at 37°C for 1 hour followed by an additional 30 minutes at 37°C with alkaline phosphatase from calf intestine (CIP) (New England Biolabs). Vector backbone and upstream and downstream segments were joined using Gibson assembly (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... according to the manufacturer’s instructions (with an elution in 30 μL sterile filtered MilliQ H2O) and restriction digest with suitable restriction enzymes (New England Biolabs) of about 250 ng plasmid DNA for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.52 and 0 nM) in a final volume of 30 μL of amplification mixture including: 1X Phi29 DNA polymerase buffer (NEB), 0.3 μM non-modified PCR forward and reverse primers ...
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bioRxiv - Microbiology 2022Quote: ... double stranded library was amplified using PCR by adding 30 μL of 2X Q5 High Fidelity Master Mix (NEB M0492L), 0.4 μL of 100 μM oDS028 ...
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bioRxiv - Biophysics 2023Quote: ... cells were labeled for 30 min at 37° C with 1 µM Surface BG-Alexa546 (impermeable dye; New England Biolabs) for SNAP in extracellular buffer (EX ...
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bioRxiv - Cell Biology 2023Quote: ... Forward and reverse primers for each sgRNA were annealed by pre-incubation at 37°C for 30 minutes with T4 polynucleotide kinase (PNK; NEB), followed by incubation at 95°C for 5 minutes and then ramp down to 25°C at 5°C/min ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
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bioRxiv - Neuroscience 2023Quote: ... with 25–30-bp overlap and used to generate plasmids via the Gibson assembly method65 using T5 exonuclease (New England Biolabs), Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: To test the binding affinity of the TBT and TBTG mRNA to the 30S ribosomal subunit we used PURExpress ΔRibosome Kit (NEB) supplemented with 5 μM of 30S ribosomal subunit and 10 μM tRNAfMet in the presence of 1.4 μM of radioactively labelled mRNA (prepared as described above by in vitro translation followed by [32P] labelling as described for the northern blot probe labelling) ...
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bioRxiv - Neuroscience 2022Quote: ... DNA fragments were amplified by PCR using primers (TSINGKE Biological Technology) with 25–30-bp overlap and then assembled using T5 exonuclease (New England Biolabs), Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 μg of total RNAs from 12-day-old Arabidopsis leaves were extracted using the Trizol method and incubated with 30 μl of pre-equilibrated oligo d(T)25 magnetic beads (NEB) for 15 minutes at room temperature with continuous rotation ...