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Citations for New England Biolabs :
801 - 850 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 5’ phosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to a blunt-end linear vector (typically also PCR products) ...
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bioRxiv - Genomics 2022Quote: ... A-tailed with Klenow 3’ to 5’ exo-(NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 U/μl T7 RNA polymerase (New England Biolabs), 1 U/μl SUPERase In (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 5 U/µl T7 RNA polymerase (NEB M0251) in 1x RNAPol buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... and 5% v/v PEG 8000 (New England Biolabs) during ligation.
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bioRxiv - Molecular Biology 2024Quote: ... 8 μl 5 U/μL Klenow Fragment (NEB, M0210S) and 2 μl T4 PNK (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 U BsmBI (New England Biolabs, Cat. No. R0580), 3 U BglII (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... then incubated with 5 units of Antarctic phosphatase (NEB) at 37°C overnight ...
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bioRxiv - Genetics 2023Quote: ... 1 ul of 5 mM 7-deaza-dGTP (NEB), 1 ul of 25 mM MgCl2 (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... +28 μl M.EcoGII at 5 U/μl (NEB M0603S). The DNA was purified using Qiagen PCR columns and quantified by measuring A260 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 5 units Antarctic Phosphatase (NEB) and 2.5 µL 10 × Antarctic Phosphatase buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... NU-1611-Cy3),1.5ul of Klenow Fragment (3’→5′ exo-, NEB, M0212S) and 1.5ul of T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 5’ phosphorylation with T4 Polynucleotide Kinase (NEB) and self-ligation with T4 DNA Ligase (NEB).
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bioRxiv - Synthetic Biology 2024Quote: ... After 5 PCR cycles using Q5 polymerase (NEB #M0491) with 5X GC Buffer with a melting temperature of 72°C ...
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bioRxiv - Biophysics 2024Quote: ... and Monarch PCR&DNA Cleanup Kit (5 μg) (NEB). The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Genomics 2022Quote: ... 5 µL of 10X VCE Buffer (New England Biolabs) and 0.5 units of yeast pyrophosphatase (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: ... coli strains: NEB 5-alpha (NEB, C2987; not authenticated), BL21-AI (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: ... 5 μL of O-glycosidase (40,000 U/μL, NEB) with 1x GlycoBuffer 3 (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs), ligated by the addition of T4 DNA ligase and transformed in E ...
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bioRxiv - Cancer Biology 2024Quote: ... and transformation (chemically competent NEB 5-alpha E. coli) were performed according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µL of 2X Luna qPCR Master Mix (NEB), 0.02 mM of forward and reverse primer ...
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bioRxiv - Molecular Biology 2024Quote: ... NEB 5-α F′Iq cells (New England Biolabs) were used as the recipient strain for plasmid construction ...
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bioRxiv - Microbiology 2024Quote: ... 1μL RNase H (5 units) (New England Biolabs, #M0297S) was added and incubated at 37°C for 20 minutes followed by an enzyme heat-inactivation at 75°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 1μL RNase H (5 units) (New England Biolabs, #M0297S) was added and incubated at 37°C for 20 minutes followed by an enzyme heat-inactivation at 75°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl vaccinia capping enzyme (NEB product number M2080), 5 μl inorganic pyrophosphate (New England Biolabs product number M2403) ...
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bioRxiv - Microbiology 2024Quote: ... was treated with 10 U RNA 5’ pyrophosphohydrolase (NEB) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... Coli NEB® 5-alpha aliquot (NEB®, #C2987H). Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... Coli NEB® 5-alpha aliquot (NEB®, #C2987H). Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5 µl of 10x T4 ligase buffer (NEB B0202S), 1 µl of T4 PNK ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 5 units of polymerase (New England Biolabs M0273). Vent reactions were performed in 1X Thermopol buffer (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 µM Mth RNA Ligase (New England Biolabs). The 100 µl master mix was split into 4 aliquots (25 µl each ...
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bioRxiv - Genomics 2024Quote: ... 5 µl 10x NEBuffer2 and 1 µl M.SssI (NEB) in a total volume of 50 µl (topped off with ddH2O) ...
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bioRxiv - Genetics 2024Quote: ... 5 µL of Exonuclease I Reaction Buffer (NEB, B0293S) was mixed with 1 µL 1:200 diluted UltraPure BSA solution (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... 5 µL of Lambda Exonuclease Reaction Buffer (NEB, B0262S) was mixed with 1 µL of nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: 5-alpha Competent E.coli High Efficiency (NEB, Cat# C2987I)
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reactions (total 30 μL) included 15 μL PhusionR High-Fidelity PCR Master Mix (New England Biolabs), 0.2 mM primers ...
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bioRxiv - Neuroscience 2020Quote: ... Vector and insert were digested (30 min, 37 °C) according to manufacturer’s protocols (enzymes by New England Biolabs), either purified directly (insert ...
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bioRxiv - Cancer Biology 2020Quote: ... on ice for 30 min and protein concentration measured by BCA assay (7780, New England Biolabs, Beverly, USA). Results were analyzed using the Compass software (ProteinSimple) ...
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bioRxiv - Plant Biology 2021Quote: ... 30 µl was removed (“input”) and the remaining sample was mixed with 50 µl of Amylose Resin (NEB) in a final volume of 500 µl ...
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bioRxiv - Microbiology 2020Quote: ... followed by 10 cycles of annealing at 54°C for 30 s) using Taq polymerase (New England Biolabs) according to the manufacturer’s instructions and the primers 5-CTTGACATCTCTGAGGCAAC-3 and 5-ATGCAGGGGCAAAGTCATTAG-3 ...
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bioRxiv - Biochemistry 2020Quote: ... The G163H (30) and G163D variants were constructed using the Q5 site-directed mutagenesis kit (New England Biolabs) to introduce the mutation ...
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bioRxiv - Developmental Biology 2020Quote: ... The probes were subjected to a 1:30 dilution of uracil-specific excision reagent (USER) enzyme (NEB, N5505S) for about 24h at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... 1:15 and 1:30 (PEI-AuNCs:siRNA)) were incubated with RNase H (10μg/ml, New England BioLabs, M0297L) at 37°C for 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... 50 µl of ULR-Phusion mix (1.2 µl ULR Ladder, 28.8 µl H2O, 30 µl Phusion PCR mastermix [New England Biolabs] and the samples were mixed with 147.5 µl ProNex chemistry ...
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bioRxiv - Cell Biology 2021Quote: ... The volume of the reaction mix was completed to 30 μl with the MetalloPhosphatase buffer (New England Biolabs) containing 1 mM of MnCl2 prior to a 30 min incubation at 30°C ...
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bioRxiv - Genomics 2021Quote: ... Nicks were then ligated for 30 min at 37 °C using 4 units of Taq DNA ligase (NEB) in the presence of 0.5 μl thermopol buffer ...
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bioRxiv - Genomics 2023Quote: ... nick ligation was then performed in a 30 µL reaction with 3 µL of 10x rCutSmart Buffer (NEB), 1.56 µL of 500 µM β-Nicotinamide adenine dinucleotide (NAD+ ...
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bioRxiv - Biophysics 2024Quote: ... Gi was incubated in 0.02% DDM/CHS on ice for 30 minutes before addition of 0.5 µl apyrase (NEB) and ScFV at a 1:1 ratio (the final concentrations of Gi and ScFV were both ∼90 µM ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The wash solution was then replaced with 1× T4 polymerase buffer containing 30 U T4 DNA polymerase (NEB) and incubated at 12 °C for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Five micrograms of Cav1.2 or Cav1.2(ΔC) and CaVβ3 cDNA were linearized by 30 units of restriction enzymes XhoI and NotI (NEB), respectively ...