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Citations for New England Biolabs :
1251 - 1300 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The enhancer was amplified by PCR to add overhangs to the backbone vector (GWLP P29-30) and assembled into the backbone by HiFi Assembly (NEB #E2621).
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bioRxiv - Molecular Biology 2023Quote: ... All mRNAs were transcribed at 30°C for 2 hrs using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) and were co-transcriptionally capped (8:1 cap analog to GTP for ∼90% capping efficiency ...
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bioRxiv - Genetics 2023Quote: ... using a biotin-labeled 30 μM dNTP mix (dATP, dGTP, dTTP and Biotin-14-dCTP Thermo Fischer, 19518018) and Klenow enzyme (NEB, M0210L) at 37°C for 80 min ...
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bioRxiv - Microbiology 2024Quote: ... Rp6 was ligated to mono-phosphorylated transcripts at 37°C for 30 min by adding T4 RNA ligase 1 (#M0204, NEB, USA) with the buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... was PCR amplified (14 cycles: 10sec 98°C, 30 sec 57°C, 20 sec 72°C) with Phusion polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Oligo pools were cloned into the vector at a 6:1 molar ratio by Gibson Assembly at 50°C for 30 minutes (NEB E2611). The plasmid pools were ethanol precipitated ...
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bioRxiv - Genomics 2024Quote: ... NaCl 5M stock was added to reach 1 M final concentration along with 30 µl of Streptavidin magnetic beads (NEB # S1420S) for 2 hrs with end-over-end mixing at 4°C to capture biotinylated DNA fragments ...
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bioRxiv - Biochemistry 2024Quote: ... was added into each ligation mixture by incubation at 30 °C for 1hour followed by adding 1 µL RecJf (NEB, M0264L) for ssDNA digestion at 37°C for 1 hour ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reaction mixtures were treated with DNase I and incubated at 37 °C for 30 minutes before immediate purification (Monarch® RNA Cleanup Kit, NEB). A sample of each mRNA was reserved for quality analysis and transcripts were tailed with E ...
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bioRxiv - Synthetic Biology 2024Quote: ... All PCR reactions were carried out in 30 μL reactions with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.3 µl of this boiled culture was used as templates in 30 µl qPCR reactions using Luna® Universal qPCR Master Mix (New England Biolabs). qPCR quantification of RT-DNA abundance was performed using a set of three primers ...
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bioRxiv - Molecular Biology 2020Quote: ... TFIIIC was recovered after overnight incubation with TEV protease (100 units, NEB). TFIIIC was empirically determined to be saturating and TFIIIB and Pol III activities were determined to be limiting by multiple-round transcription assays.
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bioRxiv - Molecular Biology 2020Quote: ... 13 μl of 10× NEBuffer3.1 and 100 units of MboI (NEB, R0147M) were added and the chromatin was digested at 37 °C overnight while shaking ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and anti-Phospho-histone H3 1:100 (New England Biolabs, Ipswich, USA) and secondary antibodies AlexaFluorTM 488 goat anti-chicken and AlexaFluorTM 594 goat anti-rabbit IgG (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... 12 μL of 10 mg/mL Bovine Serum Albumin (100× BSA, NEB), 5 μL of 400 U/μL T4 DNA Ligase (NEB M0202) ...
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bioRxiv - Genomics 2021Quote: ... was coated with 100 μL pA-M.EcoGII or commercial M.EcoGII (NEB,M0603S) (1:120 ...
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bioRxiv - Neuroscience 2021Quote: ... Beads were then incubated with 100 μl reaction buffer (containing 1X NEB heparinase mix diluted in dH2O ...
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bioRxiv - Systems Biology 2020Quote: ... We added 100 μL of T4 DNA Ligase (400 U/μL, NEB) to the basin and mixed thoroughly with the cells ...
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bioRxiv - Microbiology 2020Quote: ... and pAAVS1_c- LTatCL[M]) were linearized using 100 units of NsiI (NEB)/20 μg DNA ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplicons along with 100 bp DNA Ladder (New England Biolabs, UK) were visualised on 2% agarose gel using ethidium bromide ...
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bioRxiv - Microbiology 2020Quote: ... Other half of DNA was digested with 100 units of MboI (NEB) and ligated in the nucleus followed by in situ Hi-C protocol [42] ...
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bioRxiv - Genomics 2022Quote: ... The cells were treated with 100 U of MboI (New England Biolabs) for chromatin digestion ...
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bioRxiv - Molecular Biology 2020Quote: ... the MDA reaction was performed using 100 nM phi29 DNA Polymerase (NEB), 1x phi29 buffer (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μM ATP including 1 unit/μL T4 RNA ligase 1 (NEB) (final volume 300 μL ...
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bioRxiv - Microbiology 2020Quote: ... and 100 ng of in vitro transcribed luciferase DNA (NEB T7 HiScribe) was added to the aqueous fractions during isopropanol precipitation (52) ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng of the gene fragments were digested with BsaI-HFv2 (NEB) and purified with the Monarch PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using 100 ng of DNA and the PstI and HhaI enzymes (NEB). Paired-end reads with 150 cycles were obtained by sequencing the libraries on an Illumina Hiseq 4000 platform (Génome Québec Innovation Centre ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with 100 U of T4 DNA ligase (NEB #M0202S) for 4 hours at 16°C followed by 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The post-PCR microcapsules were treated with 100 U Exonuclease I (NEB) for 15 minutes at 37 °C ...
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bioRxiv - Genomics 2022Quote: 100-ng pTXB1 plasmids were firstly linearized by 10U XbaI (NEB, R0145S) at 37°C for 1h and purified by Zymo columns ...
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bioRxiv - Plant Biology 2022Quote: ... Amplified products and a 100-Kb DNA ladder (New England Biolabs Inc.) were visualized using agarose gel electrophoresis.
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bioRxiv - Microbiology 2024Quote: ... samples were split and treated with either 100 U Nuclease P1 (NEB) and 10 U Antarctic phosphatase ...
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bioRxiv - Cell Biology 2023Quote: ... 3 μM CaCl2) and digested with 1/100 MNase (New England Biolabs). After addition of 250 µL sonication buffer (90 mM Hepes pH 7.9 ...
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bioRxiv - Genomics 2023Quote: ... 12 μL of 10 mg/mL Bovine Serum Albumin (100 × BSA, NEB), 5 μL of 400 U/μL T4 DNA Ligase (NEB #M0202) ...
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bioRxiv - Molecular Biology 2023Quote: ... at BamHI-HF and EcoRI-HF (New England Biolabs, 100 units/μL) restriction sites following our published protocol (Bell and Keyser ...
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bioRxiv - Neuroscience 2024Quote: ... A mixture of 100 ml of RNAse Inhibitor (New England BioLabs M0314L) and 250 ml of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Systems Biology 2024Quote: ... and 14 μL Bst2.0 8,000 U/mL [100 U/mL final] (NEB) was added to the sample ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 U/ml of RNase-free DNase I (New England Biolabs, USA), 0.5 U/μl of SUPERase•In RNase Inhibitor (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... 0.5% Triton X-100) containing 8 U/mL Proteinase K (NEB #P8107S) for 12-16 hours at room temperature ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Unligated linkers were removed from the reaction by yeast 5’-deadenylase (NEB) and RecJ nuclease (NEB ...
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bioRxiv - Genomics 2020Quote: ... DNA ends were dephosphorylated by addition of 5 μL rSAP (NEB, M0203) and incubation at 37°C for 45 min ...
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bioRxiv - Microbiology 2020Quote: ... These DNA fragments were subsequently 5’ phosphorylated using T4 PNK enzyme (NEB), then cloned into a SmaI-cut pUC19 using T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were monophosphorylated on their 5’ ends using T4 polynucleotide kinase (NEB) and either 3 mM unlabeled ATP (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Obtained RNAs were 5’-radiolabelled with T4 polynucleotide kinase (New England Biolabs) and [γ32P] adenosine triphosphate (ATP ...
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bioRxiv - Molecular Biology 2020Quote: This oligonucleotide was adenylated using the 5’ DNA adenylation kit (NEB, E2610S) as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... The linkers were pre-adenylated with a 5’ DNA Adenylation kit (NEB), and then used for the ligation reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM EDTA and 40 U/ml RNAse inhibitor (New England Biolabs) at 4 °C for 5 min to remove non-specifically associated proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... triplex reaction was treated with either 5 units of RNase H (NEB) or 10εg of RNase A (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... phosphorylated by T4 polynucleotide kinase at the 5’ ends (NEB, Ipswich, MA) at 37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: ... augments Cap-specific 5’ adapter ligation by T4 RNA ligase 1(NEB). The 3’ adapter was ligated using truncated T4 RNA ligase 2 (NEB ...