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Citations for New England Biolabs :
1001 - 1050 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The ligation products were then reverse transcribed using Protoscript II reverse transcriptase (NEB) and NI-802 primer (5’-/5Phos/ NNAGATCGGAAGAGCGTCGTGTAGGGAAAGAG/iSp18/GTGACTGGAGTTCAGACGTGTGCTC-3’) ...
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bioRxiv - Genomics 2024Quote: ... Each of the 8 ligated products was transformed into electrocompetent cells (NEB #C3019), and cells were recovered in SOC medium at 37°C for 1 hr ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR product was purified with Monarch PCR DNA Cleanup Kit (NEB, T1030S) and diluted to 10 ng/uL ...
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bioRxiv - Biophysics 2024Quote: ... The digested products were ligated using T4 DNA Ligase (New England Biolabs, #M0202S). The fidelity of the chimeric clone was confirmed through diagnostic restriction digestion and direct sequencing (Eurofins ...
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bioRxiv - Genetics 2024Quote: ... The PCR product was digested with the restriction enzyme MSeI (NEB Cat no.R0525M), which resulted in two fragments (595 bp and 148 bp ...
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bioRxiv - Genetics 2024Quote: ... The resulting product was spliced into NotI-cut pUC19_HA2 by Gibson assembly (NEB). Following transformation into E ...
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bioRxiv - Biochemistry 2024Quote: ... and the PCR products were ligated with AgeI- and XhoI-(New England BioLabs) linearized pEAQ-HT vector66 using HiFi DNA assembly mix (New England BioLabs) ...
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bioRxiv - Bioengineering 2024Quote: ... The Endonuclease V-treated products were end repaired with Klenow fragment exo-(NEB), A-tailed (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... the PCR product was digested overnight at 37⁰C with PstI (NEB R0140S) and the resulting digestion products were resolved on an agarose gel.
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bioRxiv - Molecular Biology 2024Quote: ... We chemically transformed the assembled product into competent cells (New England Biolabs, #C2987H) and grew the bacteria on LB plates at 30°C overnight ...
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bioRxiv - Synthetic Biology 2021Quote: ... The linearized plasmid was then circularized using a KLD reaction (NEB) and transformed into competent cells via electroporation ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... Reactions were cycled 35 times with annealing temperatures calculated by NEB Tm calculator prior to loading on 2% agarose gels ...
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bioRxiv - Microbiology 2020Quote: ... The ligation reactions were conducted using 15 pmol of RtcB (NEB), 1x RtcB buffer (NEB) ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Reactions were carried out with OneTaq 2X master mix (NEB #M0488S) according to manufacturer instructions.
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were carried using Q5 Hot Start Polymerase (NEB, M0493S).
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bioRxiv - Microbiology 2020Quote: ... Ligation reactions were transformed into 5μl High-Efficiency DH5α cells (NEB) with heat-shock ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 μl reaction mix containing 5 μl Isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
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bioRxiv - Genomics 2020Quote: ... For all PCR reactions the Phusion HotStart Flex DNA Polymerase (NEB) was used with an annealing temperature of 55°C ...
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bioRxiv - Genomics 2020Quote: ... cells were washed in 1X T4 Ligase reaction buffer (NEB #B0202S) before being resuspended in 1X ligase (1X T4 ligase buffer ...
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bioRxiv - Microbiology 2021Quote: ... in 20μL of reaction mixture containing 1x NEB3 buffer (NEB, USA) and 1u/μL RNasin RNase Inhibitor (Promega ...
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bioRxiv - Immunology 2021Quote: ... TAP reaction was performed using 5 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Genomics 2021Quote: ... the reaction was incubated with 2 μL of USER (NEB #5508) in 1X CutSmart Buffer at 37 °C for 15 min before final purification with Zymo Oligo Clean & Concentrator kit ...
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bioRxiv - Biochemistry 2021Quote: A reaction master mix containing 1X T4 DNA Ligase Buffer (NEB) (2.5 μl/sample volume of 10X T4 DNA Ligase Buffer) ...
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bioRxiv - Microbiology 2020Quote: ... and the assembly reaction used to transform E.coli (NEB 10 beta). Colonies positive by PCR screening using primers that flank the cloning site were sequenced across the entire S coding region and a single positive isolate adopted for all further manipulations.
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions were set-up using Luna qPCR Master Mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... The PCR reaction contained 25 μl 2x Q5 DNA Polymerase (NEB), 2.5 μl K1F (10 μM) ...
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bioRxiv - Genetics 2022Quote: ... The PCR reaction contained 25 μl 2x Q5 DNA Polymerase (NEB), 2.5 μl K2.F (10 μM) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... reaction was stopped by adding 2X RNA gel-loading buffer (NEB) with urea ...
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bioRxiv - Microbiology 2021Quote: ... Following circularization of both intermediate constructs using KLD reaction buffer (NEB), a region comprising sgRNA1 was PCR amplified with primers 6 and 7 from pSag1::Cas9-U6::sg1PDE1 and Gibson assembled into Kpn1/XhoI linearised pSag1::Cas9-U6: ...
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bioRxiv - Microbiology 2021Quote: ... Following circularization of both intermediate constructs using KLD reaction buffer (NEB), a region comprising sgRNA1 was PCR amplified with primers 6 and 7 from pSag1::Cas9-U6::sg1CDPK3 and Gibson assembled into Kpn1/XhoI linearised pSag1::Cas9-U6: ...
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bioRxiv - Developmental Biology 2020Quote: ... immediately followed by the second blunting reaction using ExoT (NEB, M0265S) for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μl reaction mix containing 5 μl Isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
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bioRxiv - Genomics 2020Quote: ... 2 μL 5x Q5 reaction buffer (New England Biolabs, Ipswich, MA), 0.2 μL 10 mM dNTPs (Kapa Biosystems ...
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bioRxiv - Bioengineering 2021Quote: ... Golden Gate reactions were performed in 1X T4 ligase buffer (NEB) with 1000 units of T4 DNA Ligase (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: The pEntr-EMS plasmid was generated from a HiFi reaction (NEB) using the pEntr3c Gateway cloning (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... One 20 μl ligation reaction using T4 ligase (New England Biolabs) was carried out using 0.9 ng of the gel-purified insert and 500 ng of the vector ...
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bioRxiv - Biochemistry 2022Quote: ... the reaction was treated with 1μl of DpnI (BioLabs, New England) for 1 hour at 37°C before transformation ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μl 5X Phusion® HF Reaction Buffer (New England BioLabs), 1 U of Phusion® High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μl 5X Phusion® HF Reaction Buffer (New England BioLabs), 1 U of Phusion® High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... Reactions contained 1.0 U Q5 high fidelity DNA polymerase (NEB, UK), 0.25 μM primers ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription reaction with 0.2 U/μL AMV Reverse Transcriptase (NEB) was performed at 42°C for 30 min using a dNTP/ddCTP mix at 0.25 mM each (final concentration) ...
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bioRxiv - Molecular Biology 2021Quote: ... NEBNext Quick Ligation Reaction Master Mix (New England Biolabs, Cat # B6058), Oxford Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Polymerase chain reactions (PCRs) were performed using Phusion DNA polymerase (NEB) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... The 25□µL final reaction volume contained 1X PCR Buffer (NEB), 3□mM MgCl2 ...
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bioRxiv - Genomics 2020Quote: ... 5 μl 5x Q5 reaction buffer (New England Biolabs, Ipswich, MA), 0.5 μL 10 mM dNTPs (Kapa Biosystems ...
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bioRxiv - Genomics 2020Quote: ... 5 μL 5x Q5 reaction buffer (New England Biolabs, Ipswich, MA), 0.5 μL 10 mM dNTPs (Kapa Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... 400 μM ATP (1x T4 RNA ligase 2 reaction buffer (NEB)) including 0.25 units/μL T4 RNA ligase 2 (Neb ...
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bioRxiv - Developmental Biology 2021Quote: ... The dephosphorylation reaction was supplemented with 0.5 µL T4 PNK (NEB) and the reaction was incubated for 25 minutes ...
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bioRxiv - Pathology 2021Quote: ... RT-LAMP reactions were performed using WarmStart LAMP Kit (NEB, USA) at a final volume of 10 μL ...