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Citations for New England Biolabs :
901 - 950 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The colony PCR amplified products were digested with AvrII (New England Biolabs R0174S) in CutSmart buffer at 37°C for 3 hours before being resolved with agarose gel electrophoresis.
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bioRxiv - Developmental Biology 2021Quote: ... 200 ng of PCR products in 1X NEB buffer 2 (New England Biolabs) were hybridized under the following conditions ...
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bioRxiv - Genomics 2020Quote: ... and the resulting product was transformed into 10-Beta Electrocompetent cells (NEB C3020K). We plated 1% of the library to estimate complexity and grew the rest of the sample and then midi prepped (Zymo D4200 ...
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bioRxiv - Immunology 2020Quote: ... The product and backbone were digested with HindIII-HF and NotI-HF (NEB), purified ...
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bioRxiv - Molecular Biology 2021Quote: The 5’ RACE PCR product was digested using BamHI (New England Biolabs R0136) and EcoRV (New England Biolabs R3195 ...
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bioRxiv - Biophysics 2021Quote: ... The PCR product was digested with the DraIII restriction enzyme (New England BioLabs) for 4 hours at 37 °C to generate a 3-N-43 fragment with a 3 nucleotide (nt ...
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bioRxiv - Bioengineering 2021Quote: ... 10 ng of purified PCR products were incubated with I-SceI endonuclease (NEB) according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... 50 ng of the PCR product was ligated with T4 DNA ligase (NEB) in a final volume of 10 μL overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The amplified PCR product was digested by MluI and NheI restriction enzymes (NEB), and was ligated into the pVSV-FL+(2 ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified and in vitro transcribed using T7 HiScribe kit (NEB). Transcribed RNA products were run on a 5% acrylamide TBE-urea gel and visualized with 0.02% methylene blue in 1x TBE ...
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bioRxiv - Synthetic Biology 2021Quote: ... The resulting PCR products were self-assembled using T4 ligase (New England Biolabs) to obtain the desired SSGM plasmid libraries ...
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bioRxiv - Immunology 2021Quote: ... PCR products were generated using Phusion High-Fidelity DNA Polymerase (New England Biolabs) or Herculase II Fusion DNA Polymerase (Agilent Technologies) ...
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bioRxiv - Biophysics 2022Quote: ... The final product was purified by Monarch RNA Cleanup kit (New England Biolabs), eluted in water ...
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bioRxiv - Molecular Biology 2022Quote: ... The remaining circularized products were amplified by PCR using Phusion polymerase (#M0530L, NEB). In a pilot experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were cleaned up using the Monarch PCR Cleanup kit (T1030L, NEB). A double digest using PacI and AscI was done on the PCR products and the pcs2+MCS-P2A-sfGFP plasmid ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... and PCR products were recircularised using KLD enzyme blend (New England Biolabs (UK)) ...
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bioRxiv - Cell Biology 2022Quote: ... The purified RT-PCR products were double digested by BamHI and XbaI (NEB) and then ligated into pBiFC-Flag-VN or -YC vectors by T4 DNA ligase or when these two restriction enzymes had cut sites within the cDNA sequences ...
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bioRxiv - Microbiology 2020Quote: ... PCR products recombined using the NEBuilder HiFi DNA assembly kit (New England Biolabs). All constructs were verified by sequencing.
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bioRxiv - Microbiology 2020Quote: ... The PCR product was cloned into the pMAL-p5x vector (New England Biolabs) using the XmnI and BamHI restriction sites (underlined ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of each PCR product was digested with HaeIII (New England Biolabs) at 37°C for 60 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Products were purified using the Monarch DNA gel purification kit (New England Biolabs), and Sanger sequenced.
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bioRxiv - Evolutionary Biology 2022Quote: ... was performed with PCR products and pCFD4 BbsI (New England Biolabs, Cat. # R0539L) digested vector ...
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bioRxiv - Molecular Biology 2022Quote: ... and the products were gel-purified using the Monarch Gel Purification Kit (NEB) following the manufacturer’s instructions and eluted in 40 μL H2O ...
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bioRxiv - Bioengineering 2022Quote: ... PCR product was purified and digested with EcoRI and SacI (New England Biolabs). The adhB_pdc operon that encodes AdhB alcohol dehydrogenase and Pdc pyruvate decarboxylase from Zymomonas mobilis with synthetic ribosome binding sites was synthesized by Genewiz ...
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bioRxiv - Bioengineering 2020Quote: ... The purified ligation product was then digested by DraI (New England Biolabs, R0129S) and a fragment of ~680 bp in size was purified by DNA agarose gel extraction to get the final VHH library ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB) and sequenced using the Sanger method with M13F or M13R primers.
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bioRxiv - Bioengineering 2021Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). RT ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB) and purified with RNEasy Kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... Mutagenesis PCR product was then digested overnight using DpnI (New England Biolabs, R0176) followed by bacterial transformation into competent cells ...
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bioRxiv - Genetics 2020Quote: ... PCP products were used as template for T7 endonuclease assay (New England BioLabs), following manufacturer instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Gel-purified PCR products were A-tailed using Taq polymerase (New England Biolabs) for 30 min at 72 °C ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... the PCR products were further digested with NcoI (New England Biolabs, Cat# R3193S) at 37°C for 1 h and then ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The ligation product was purified using the Monarch PCR & DNA Cleanup Kit (NEB) eluting the product in 10 μL ...
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bioRxiv - Microbiology 2021Quote: ... The product was digested using the KLD Enzyme Mix reagents (New England Biolabs) and transformed into DH5-alpha competent cells ...
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bioRxiv - Microbiology 2020Quote: ... The linearized PCR product was dephosphorylated using Shrimp Alkaline phosphatase (New England Biolabs) per the manufacturer’s instructions and then ligated with tetL to generate the deletion construct PB408 ...
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bioRxiv - Genetics 2020Quote: ... This intermediate plasmid product (pLCv2-GFP) was digested with AfeI (NEB, Cat# R0652S) and EcoRI-HF (NEB ...
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bioRxiv - Genomics 2020Quote: ... Pooled PCR products were subjected to 1U/μl Not1 HF (New England BioLabs) endonuclease digestion at 37°C for one hour ...
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bioRxiv - Cell Biology 2021Quote: ... and the products were digested with Taq I (New England Biolabs, Evry, France) that could recognize CpGs ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid and insert products were digested using appropriate restriction enzymes (New England Biolabs) and ligated using T4 Ligase (New England Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... Overlap extension PCR products were digested using NheI and MluI (New England Biolabs) and ligated into the yeast surface display vector VLRB.2D-aga2 digested with these same two enzymes ...
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bioRxiv - Cell Biology 2022Quote: ... The product was subsequently digested with the restriction enzyme BtsI-v2 (NEB, #R0667S), which has a restriction site present only in the sa659 allele.
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bioRxiv - Genomics 2022Quote: ... after which the resulting PCR product was treated with DpnI (New England Biolabs) and PCR-purified ...
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bioRxiv - Genomics 2022Quote: ... The bisulfite converted product was amplified using NEBNext Q5U Master Mix (NEB, M0597). The resulting indexed library was used for Illumina sequencing or target enrichment.
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bioRxiv - Microbiology 2022Quote: ... Ligation between these digested products was performed using T4 DNA ligase (M0202S, NEB). 50μl of competent bacteria were next transformed with 5μl of the ligation reaction product ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were digested with RsaI or ApoI-HF (NEB Inc; Ipswich, MA) and were separated on 1.5% agarose gels ...
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bioRxiv - Microbiology 2022Quote: ... Linear products were phosphorylated with T4 Polynucleotide Kinase (New England Biolabs, MA, USA) for 1 hr at 37°C and circularized with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... the linear PCR product was phosphorylated using T4 polynucleotide kinase (New England Biolabs) and ligated with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... purified PCR products were cloned using NEB PCR Cloning Kit (New England BioLabs). Clones were Sanger sequenced at the University of Minnesota Genomics Center.