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Citations for New England Biolabs :
801 - 850 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The resulting product was transformed into stable chemically competent E.coli (NEB C3040H) and plated ...
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bioRxiv - Bioengineering 2021Quote: ... The obtained PCR products were subsequently treated with DPNI (New England Biolabs) and cleaned up with the Qiaquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... The ligated product was then transformed into E.coli 10beta (New England Biolabs) and colonies were grown on LB-agar plate containing kanamycin for selection ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR product was digested using restriction enzyme PspGI (New England Biolabs), which cleaves wildtype product into 131bp and 157bp fragments ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Gibson assembly products were transformed into high-efficiency chemically competent DH5α (NEB) with 30 minutes of heat shock recovery ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was then added to a KLD enzyme mix (NEB) reaction and was incubated at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested with EcoRI and BamHI (New England Biolabs), and ligated into pmCherry-N1 and pEGFP-N1 plasmids that had been digested with the same restriction enzymes.
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bioRxiv - Plant Biology 2021Quote: ... PCR products were generated using Q5® High- Fidelity DNA Polymerase (NEB) and column-purified by using GeneJET PCR Purification Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was digested with restriction enzymes (New England Biolabs, NEB), followed by ligated to linearized plasmid using T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was digested with restriction enzymes (New England Biolabs, NEB), followed by ligated to linearized plasmid using T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2021Quote: All PCR products were synthesized using Phusion HF polymerase (New England Biolabs). Sequencing of constructs and strains was performed at Eurofins Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were loaded with 6X purple gel loading dye (B7025, NEB) and electrophoresed alongside a low molecular weight ladder (range 25 to 766 base pairs ...
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bioRxiv - Genomics 2022Quote: ... The cleaned RCA product was digested using NEBNext dsDNA Fragmentase (NEB #M0348) following the manufacturer protocol with a 10 minute incubation ...
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bioRxiv - Microbiology 2022Quote: ... the PCR product was digested with NdeI and HindIII (NEB, Ipswich, MA). The digested product was then ligated with a NdeI and HindIII cut pCT94 using Gibson assembly (NEB ...
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bioRxiv - Genomics 2022Quote: ... PCR products were purified by gel extraction (Monarch gel extraction kit, NEB), and assembled into the backbones using NEB HiFi DNA Assembly master mix kit ...
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bioRxiv - Biochemistry 2022Quote: ... Following which the PCR products were purified by PCR cleanup kit (NEB) and a second PCR was done with primers containing the READ1 ...
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bioRxiv - Microbiology 2022Quote: ... 20 μl of nested PCR product was digested using AvaII (NEB, R0153S) for 7-12 h at 37°C in CutSmart buffer (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The AQ-HOXDB_5 PCR product was digested with BssSI-v2 (NEB, R0680L), and the AQ-HOXDB_6 PCR product was digested with NdeI (Thermo Scientific FD0583) ...
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bioRxiv - Bioengineering 2021Quote: ... PCR products were purified by gel extraction (Monarch gel extraction kit, NEB), and assembled in a Golden Gate assembly containing 6.25 ng pU6-atgRNA-GG-acceptor (Addgene #132777) ...
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bioRxiv - Biophysics 2021Quote: ... The PCR product was digested with PspGI restriction enzyme (NEB, catalog #R0611S) to obtain a 1080 bp long DNA with 5 bp overhang and was subsequently dephosphorylated with Antarctic phosphatase (NEB ...
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bioRxiv - Genetics 2022Quote: ... The digested products were ligated together with T4 ligase (New England Biolabs). An aliquot of the ligation mix was transformed into DH5α competent cells by heat shock (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... The circRNA products were further purified using Monarch RNA Cleanup Kit (NEB). The quality and quantity of RNA were evaluated by agarose gel electrophoresis and Qubit RNA HS Assay Kit (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... 5 μL of ligation products were then transformed into competent cells (NEB). Transformation and plasmid DNA recovery were performed as previously described ...
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bioRxiv - Cell Biology 2020Quote: ... Annealed product was ligated into digested vector using T4 DNA ligase (NEB). The ligated plasmid was transformed into DH5α competent bacteria ...
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bioRxiv - Immunology 2020Quote: ... 1.4 μg PCR products were mixed with 50U T7 RNA polymerase (NEB), 1 U RNase inhibitor (NEB) ...
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bioRxiv - Genetics 2020Quote: ... The PCR products and the T444T vector were digested with KpnI (NEB) restriction enzyme for all C ...
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bioRxiv - Cancer Biology 2021Quote: ... or enzymatic methylation conversion (Enzymatic Methyl-seq Conversion Module, NEB Product #E7125L) to convert unmethylated cytosine to uracil ...
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bioRxiv - Synthetic Biology 2021Quote: ... of PCR products of the vector (NEB Phusion High-Fidelity DNA Polymerase) using primers pBEST_GA_1_F ...
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bioRxiv - Developmental Biology 2020Quote: ... the purified PCR product was used for in vitro transcription (NEB, E2040S) followed by reverse transcription (Thermo Fisher ...
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bioRxiv - Genetics 2020Quote: ... PCR products were first amplified using Phusion High-Fidelity DNA Polymerase (NEB). The avi-tag was sub cloned in pBSSK (EcoRI/Hind III 0.18-kb) ...
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bioRxiv - Biochemistry 2021Quote: ... 50% of the resulting product was digested with XbaI (New England Biolabs) (3 h ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were generated using the 2x Q5 PCR Master Mix (NEB) in accordance with manufacturer instructions ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Resulting PCR product was purified using Monarch PCR purification kit (NEB, USA) and were cloned into pCS2+ vector using restriction enzymes BamH1 and SnaB1 based cloning to generate pCS2-Satb2 ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA product was digested with TspRI in 1X CutSmart Buffer (NEB) overnight at 65 °C to generate the 9-nt ssDNA overhang ...
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bioRxiv - Molecular Biology 2023Quote: ... and ligating both products with the T4 DNA Ligase (New England Biolabs). Plasmid YEplac181-Sin3-(HA)3 (2µ AmpR LEU2 SIN3- (HA)3 ...
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bioRxiv - Genomics 2023Quote: ... The resulting PCR product was then circularized by treatment with PNK (NEB) and T4 ligase (NEB ...
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bioRxiv - Genetics 2023Quote: ... and PCR products were then purified by PCR & DNA Cleanup Kit (NEB). STR PCR products were ligated with the pClone007 simple vector (Tsingke) ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA cleavage products were purified using Monarch RNA Cleanup kit (NEB) and divided into two parts ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA cleavage products were purified using Monarch RNA Cleanup kit (NEB) and used for a one-pot reaction of end-prep and splint annealing ...
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bioRxiv - Systems Biology 2023Quote: ... Spin-purified PCR products were digested with BsaI-HF-v2 (R3733; NEB) and the size and integrity of full length and digested PCR products were confirmed on a 4% agarose e-gel (Thermo) ...
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bioRxiv - Genetics 2023Quote: ... Spin-purified PCR products were digested with BsaI-HF-v2 (R3733; NEB) and the size and integrity of full length and digested PCR products were confirmed on a 4% agarose e-gel (Thermo) ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR products were treated with a KLD enzyme mix (NEB M0554S), which phosphorylates the 5’ ends of the linearized PCR products ...
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bioRxiv - Neuroscience 2023Quote: ... All PCR products were amplified using a standard Q5 polymerase protocol (NEB) and purified with an agarose gel extraction kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Digestion of this PCR product with PvuII-HF (New England Biolabs, www.neb.com) distinguished between SICm (PvuII sensitive ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleaved genomic DNA product was treated with NEBNext dA-tailing kit (NEB) and subjected to library preparation using nanopore adapter (ONT SQK-LSK109 ...
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bioRxiv - Immunology 2023Quote: ... The PCR product was phosphorylated and ligated with KLD enzyme mix (NEB) before transformation into competent cells followed by sequence verification (1st BASE ...
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bioRxiv - Microbiology 2023Quote: ... All Sanger sequencing products were generated with Q5 High-Fidelity polymerase (NEB), Phusion Hot Start Flex DNA polymerase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR product was cut with BamH1 and Xho1 (New England Biolabs) and cloned directly into pCS2R cut with the same enzymes ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μL of ligation product was then transformed into competent cells (NEB). Transformation and plasmid DNA recovery were performed as previously described ...
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bioRxiv - Genomics 2022Quote: ... The products were eluted from the NEBNext polyA magnetic isolation (NEB, E7490S) in tris buffer ...