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Citations for New England Biolabs :
951 - 1000 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were carried out using the Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were selected by 2% agarose gel electrophoresis ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR reaction was performed using NEBNext High Fidelity PCR Master Mix (NEB, catalog no. M0541L) for 20 cycles ...
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bioRxiv - Biochemistry 2023Quote: PCR mutagenesis was performed by whole plasmid PCR with Q5 high-fidelity polymerase (New England Biolabs) followed by PCR purification (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Each PCR consisted of: 20 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 1 μL 10 μM forward primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... General PCR for cloning was performed using the NEBNext PCR master mix (NEB cat no. M0541L), with 500 nM of forward and reverse primers ...
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bioRxiv - Genetics 2024Quote: ... and libraries were generated by PCR with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541). Prior to sequencing ...
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bioRxiv - Biophysics 2024Quote: ... PCR was followed by the addition of the PCR product to a KLD enzyme mix (NEB) for template removal ...
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bioRxiv - Genetics 2021Quote: ... using Q5 polymerase PCR products (NEB). pCMV_AncBE4max (Addgene plasmid #112094 ...
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bioRxiv - Biochemistry 2019Quote: ... The PCR amplification (Q5 polymerase (NEB)) consisted of 30 cycles (95°C ...
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bioRxiv - Genomics 2020Quote: ... 50μl PCR reactions using Q5 (NEB) were cycled 98°C 1min ...
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bioRxiv - Genetics 2021Quote: ... PCR reagents were purchased from NEB, and all primers were synthesized by Integrated DNA Technologies (IDT).
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bioRxiv - Microbiology 2021Quote: ... PCR amplification by Taq polymerase (NEB) using primers for internal regions of KL ...
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bioRxiv - Neuroscience 2021Quote: ... Luna qRT-PCR Master Mix (NEB) was used with the primers ...
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bioRxiv - Plant Biology 2021Quote: ... PCR using Standard Taq Polymerase (NEB) was performed for 30 cycles of (denaturation ...
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bioRxiv - Synthetic Biology 2021Quote: Phusion PCR reactions (New England Biolabs) were used to generate backbone fragments for library assembly ...
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bioRxiv - Synthetic Biology 2021Quote: Phusion PCR reactions (New England Biolabs) were used to generate amplicons for Illumina NGS from purified plasmid DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... by PCR with Q5 polymerase (NEB) using primers petSUMO_F ...
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bioRxiv - Molecular Biology 2021Quote: ... NEB Phusion PCRs (NEB; Cat #: M0531S) were conducted from reverse transcriptase reactions generated from the SuperScript™ VILO™ cDNA Synthesis Kit (Thermofisher ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions used Phusion polymerase (NEB) or PrimeStarGXL polymerase (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... Luna qRT-PCR Master Mix (NEB) was used with the primers ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was performed with OneTaq (NEB), following the manufacturer’s specifications ...
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bioRxiv - Evolutionary Biology 2021Quote: ... High-fidelity PCR Master Mix (BioLabs) and a touch-down annealing process were used for amplification ...
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bioRxiv - Microbiology 2020Quote: ... and OneTaq PCR Master Mix (NEB). Reverse primers for most sgRNAs also contained a unique KpnI or BglII site to assist clone identification by restriction fragment analysis ...
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bioRxiv - Microbiology 2021Quote: ... After PCR with Q5 polymerase (NEB), the product was treated with DpnI (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... Phusion PCR reactions (New England Biolabs) were used to amplify these extended oligos ...
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bioRxiv - Systems Biology 2022Quote: ... and PCR master mix (NEB, M0543S). We used 12 cycles to amplify the cDNA using the following protocol ...
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bioRxiv - Systems Biology 2022Quote: ... and PCR Master Mix (NEB, M0543S) for each reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... OneTaq RT-PCR mix (NEB #E5315) was used to amplify the generated cDNA.
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification using Phusion polymerase (NEB) with selective primes (fwd ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA libraries underwent PCR amplification (NEB-Next High-Fidelity 2x PCR Master Mix ...
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bioRxiv - Systems Biology 2023Quote: ... and PCR master mix (NEB, M0543S). The amplification began with a 30-second denature step at 98°C ...
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bioRxiv - Cell Biology 2023Quote: ... Following PCR using Q5 polymerase (NEB), the product is gel-purified on a 1% agarose gel ...
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bioRxiv - Cell Biology 2023Quote: All fragments were PCR purified (NEB), ligated using NEB HiFi assembly kit ...
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bioRxiv - Plant Biology 2024Quote: ... amplified by Phusion PCR (NEB, UK).
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bioRxiv - Molecular Biology 2021Quote: ... Complementary DNA (cDNA) was generated by reverse transcriptase polymerase chain reaction (PCR) using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, Ipswich, MA). Transcript levels were measured by real time PCR using SYBR green on an ABI 7500 system ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Microbiology 2019Quote: ... asparagine 36 and 484 were replaced by glutamine in N-glycosylation sites through PCR using the Gibson Assembly® Cloning Kit (New England BioLabs, Frankfurt, Germany) with primers PDI1Asn36mut-1 and PDI1Asn36mut-2 for the mutation N36Q and primers PDI1Asn484mut-1 and PDI1Asn484mut-2 for the mutation N484Q ...
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bioRxiv - Plant Biology 2022Quote: ... and pCambia1305.1 vectors were prepared and subsequently digested using NcoI restriction endonuclease prior to purification using Monarch DNA & PCR Cleanup Kits (New England Biolabs, Ipswich, MA, USA). Vector and insert concentrations were estimated using a Synergy H1 microplate reader (BioTek Instruments ...
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bioRxiv - Developmental Biology 2022Quote: ... Products were visualized on 2% agarose gels and successful amplification reactions were prepared for Sanger sequencing with Exo-CIP™ Rapid PCR Cleanup Kit according to the manufacturer’s protocol (New England Biolabs: E1050S, Ipswich, MA). Samples were then sequenced with amplification primers (Eurofins Genomics Services ...
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bioRxiv - Biochemistry 2022Quote: ... The two pairs of PCR products were ligated together by in vitro homologous recombination using a Gibson assembly cloning kit (NEB, Boston, MA, USA), respectively ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Some libraries have been instead prepared with the NEBNext Ultra II DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Samples were either double indexed with a set of 60 dual-index primers ...
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bioRxiv - Microbiology 2023Quote: ... The upstream and downstream PCR products were designed to have an overlapping region of sequence to promote 3-part Gibson assembly (NEB Gibson assembly kit) and primers 5b and 4 had extensions to anneal to vector pMP62 ...
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bioRxiv - Genetics 2023Quote: ... COL3A1-5’ UTR fragment was created from the reference allele (TT) PCR product using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs, Massachusetts, USA). The sequence of primers carrying out the alternative allele ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription and quantitative PCR were performed in one step using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA). The primers used are listed in supplemental table (Table ...
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bioRxiv - Cell Biology 2023Quote: ... Mammalian cell lines were handled under sterile conditions using a laminar flow hood and the cells were regularly tested for mycoplasma contamination with a PCR-based mycoplasma detection kit (Minerva-Biolabs, cat.no. 11-1250). Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was used as the template for IVT with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA). Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... dilution of the universal adaptor and 13 cycles (or 16 cycles) of PCR per the respective masses with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Cat. No. E7760L), the NEBNext Poly(A ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed in a 10 µl reaction volume containing 1X PCR Buffer (New England Biolabs), 10 µM dNTP and 0.05 U Taq DNA polymerase (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...