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Citations for New England Biolabs :
901 - 950 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... and PCR Master Mix (NEB, M0543S) for each reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... OneTaq RT-PCR mix (NEB #E5315) was used to amplify the generated cDNA.
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bioRxiv - Cell Biology 2023Quote: ... Following PCR using Q5 polymerase (NEB), the product is gel-purified on a 1% agarose gel ...
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bioRxiv - Cell Biology 2023Quote: All fragments were PCR purified (NEB), ligated using NEB HiFi assembly kit ...
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bioRxiv - Systems Biology 2023Quote: ... and PCR master mix (NEB, M0543S). The amplification began with a 30-second denature step at 98°C ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA libraries underwent PCR amplification (NEB-Next High-Fidelity 2x PCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification using Phusion polymerase (NEB) with selective primes (fwd ...
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bioRxiv - Molecular Biology 2021Quote: ... Complementary DNA (cDNA) was generated by reverse transcriptase polymerase chain reaction (PCR) using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, Ipswich, MA). Transcript levels were measured by real time PCR using SYBR green on an ABI 7500 system ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Microbiology 2019Quote: ... asparagine 36 and 484 were replaced by glutamine in N-glycosylation sites through PCR using the Gibson Assembly® Cloning Kit (New England BioLabs, Frankfurt, Germany) with primers PDI1Asn36mut-1 and PDI1Asn36mut-2 for the mutation N36Q and primers PDI1Asn484mut-1 and PDI1Asn484mut-2 for the mutation N484Q ...
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bioRxiv - Plant Biology 2022Quote: ... and pCambia1305.1 vectors were prepared and subsequently digested using NcoI restriction endonuclease prior to purification using Monarch DNA & PCR Cleanup Kits (New England Biolabs, Ipswich, MA, USA). Vector and insert concentrations were estimated using a Synergy H1 microplate reader (BioTek Instruments ...
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bioRxiv - Developmental Biology 2022Quote: ... Products were visualized on 2% agarose gels and successful amplification reactions were prepared for Sanger sequencing with Exo-CIP™ Rapid PCR Cleanup Kit according to the manufacturer’s protocol (New England Biolabs: E1050S, Ipswich, MA). Samples were then sequenced with amplification primers (Eurofins Genomics Services ...
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bioRxiv - Biochemistry 2022Quote: ... The two pairs of PCR products were ligated together by in vitro homologous recombination using a Gibson assembly cloning kit (NEB, Boston, MA, USA), respectively ...
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bioRxiv - Microbiology 2023Quote: ... The upstream and downstream PCR products were designed to have an overlapping region of sequence to promote 3-part Gibson assembly (NEB Gibson assembly kit) and primers 5b and 4 had extensions to anneal to vector pMP62 ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription and quantitative PCR were performed in one step using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA). The primers used are listed in supplemental table (Table ...
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bioRxiv - Genetics 2023Quote: ... COL3A1-5’ UTR fragment was created from the reference allele (TT) PCR product using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs, Massachusetts, USA). The sequence of primers carrying out the alternative allele ...
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bioRxiv - Cell Biology 2023Quote: ... Mammalian cell lines were handled under sterile conditions using a laminar flow hood and the cells were regularly tested for mycoplasma contamination with a PCR-based mycoplasma detection kit (Minerva-Biolabs, cat.no. 11-1250). Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Some libraries have been instead prepared with the NEBNext Ultra II DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Samples were either double indexed with a set of 60 dual-index primers ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed in a 10 µl reaction volume containing 1X PCR Buffer (New England Biolabs), 10 µM dNTP and 0.05 U Taq DNA polymerase (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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bioRxiv - Microbiology 2021Quote: ... All PCR steps were carried out using the Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were electrophoresed on a 2% agarose gel and stained with SYBR Safe DNA gel stain ...
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bioRxiv - Systems Biology 2022Quote: ... PCR amplification was performed on the eluate with an NEBNext 2X High Fidelity PCR Mix (NEB, M0541S) N7 ...
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bioRxiv - Genetics 2022Quote: ... Phusion® High-Fidelity PCR Master Mix was used to prepare the PCR library (New England Biolabs). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... Random mutagenesis was performed using Error-Prone PCR (EP-PCR) with Taq DNA polymerase (New England Biolabs). PCR products and restriction digestion products were purified using a GeneJET gel extraction kit (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1×loading buffer (contained SYBR green ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion®High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1X loading buffer (contained SYB green ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs), and the mixture PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... the scFv containing region from isolated plasmids was amplified by PCR using Q5 high fidelity PCR (NEB). Illumina NGS samples were prepped and run using the Illumina MiSeq v3 reagent kit following manufacturer’s protocols pooling four library samples per lane ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was done with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Genomics 2021Quote: ... first-level PCR reactions were performed using 1μl PCR-compatible lysate as a template for a 6.25μl Q5 (NEB) PCR reaction according to the manufacturer’s protocol (annealing temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs) and purified using Agencourt AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Synthesized testis cDNAs were used for endpoint PCR or quantitative PCR using OneTaq 2X Master Mix (NEB) or with iTaq Universal SYBR Green Supermix (BIO-RAD) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragments of interest were treated with the same enzymes and cleaned by PCR cleanup (NEB). Linear plasmid pJAM2678 and each PCR product were ligated together in a reaction using T4 DNA ligase overnight at 16 °C to obtain plasmid pAKS202 that contained 150 bp upstream of the translation start site of hvo_B0047 cloned in front of bgaH ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Preamp PCR: Beads were resuspended in 200 µl PCR mix (20 µl 5x phusion HF buffer (NEB); 1 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... and PCRs were performed via Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB #M0531S). After PCR clean-up (Promega #A9281 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitive PCR and limited-cycle PCR was performed using NEBNext Ultra II master mix (New England Biolabs), SYBR™ green and 0.5 µM of sample-specific P5/P7 barcoded primer mix (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were generated using the OneTaq Quick-Load 2x PCR Master Mix with Standard Buffer (NEB) in accordance with manufacturer instructions with C ...
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) or RedTaq DNA Polymerase 2X Master Mix (VWR Life Science ...
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bioRxiv - Immunology 2023Quote: ... 17 PCR cycles were performed using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532) with primers and template oligo each at a concentration of 10uM ...
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bioRxiv - Biochemistry 2024Quote: ... Acad11 genotypes were assessed using a three-primer PCR strategy with the 5X Multiplex PCR Mix (NEB) to allow for the simultaneous detection of both wild-type and mutant alleles ...
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bioRxiv - Genetics 2023Quote: ... The purified DNA was then amplified by PCR using NEBNext HiFi 2x PCR Master Mix (NEB M0541S), and the resulting library was purified using 1.3X SPRI purification (Omega Bio-Tek ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were amplified by PCR with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs #174M0541S) for 9 cycles in 96-well Thermal cycler (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed from 2.5 ng of DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 9 PCR cycles (NEB #E7645S, New England Biolabs). Library quality was assessed by High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Genetics 2021Quote: ... The PCR product was inserted to the PstI and BglII sites on the pWALIUM20 vector (Perkins 2015) by Gibson-assembly (NEB HiFi DNA Assembly Kit) to generate pWALEXA20 (White-AttB-LexAop for shRNA expression) ...
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bioRxiv - Microbiology 2022Quote: ... The extracted DNA from water and soil underwent a further purification step using Monarch®PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA). DNA Library preparation was conducted using Native barcoding genomic DNA Kit SQK-LSK 109 combined with EXP-NBD104 (Oxford Nanopore Technologies ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...