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Citations for New England Biolabs :
51 - 100 of 215 citations for Oleoyl Gly Lys m PEG11 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 0.7 µl T4 ligase (2 M U/ml, NEB), 250 ng DNA (adapters in File S1 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was incubated with M-MLV reverse transcriptase (NEB) with 10 mM dithiothreitol (DTT) ...
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bioRxiv - Neuroscience 2023Quote: ... 100 U of M-MLV Reverse Transcriptase (NEB; M0253S), M-MLV RT buffer (NEB ...
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bioRxiv - Bioengineering 2023Quote: HindIII-HF restriction enzyme (NEB cat. no. R3104T/M)
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using M-MuLV Reverse Transcriptase (NEB) with gene-specific primers and OligoDT (20mer+5’Phos ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using M-MuLV Reverse Transcriptase (NEB) with minus-strand specific primers (IDT) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 2.5 U/µL M-MuLV RT (NEB #M0253L) unless indicated otherwise ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in spheroblast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 μM beta-mercaptoethanol) and digested with 0.002% 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were resuspended in 1 mL spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 µM beta-mercaptoethanol), and 1.2–5 µL 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Bioengineering 2022Quote: ... Processing the samples for proteomics was then continued using a 10 kDa filter aided sample preparation protocol previously published with minor modifications.[30] The proteins were digested using Lys-C enzyme (P8109S, New England Biolabs, MA, USA; 1:50) and then with trypsin (V5111 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Reverse transcription was performed using M-MulV Reverse Transcriptase (NEB) with 250 ng of total RNA using gene specific primers for Figure S11 and using an oligo-T primer for Figure 4e ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Lane M is DNA marker (NEB 1 Kb DNA Ladder), lane N shows the PCR product from negative control (ddH2O) ...
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bioRxiv - Bioengineering 2023Quote: SalI restriction enzyme (NEB cat. no. R0138S/T/L/M)
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bioRxiv - Bioengineering 2023Quote: KpnI-HF restriction enzyme (NEB cat. no. R3142S/L/M)
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bioRxiv - Bioengineering 2023Quote: T4 DNA Ligase (NEB cat. no. M0202S/T/L/M)
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bioRxiv - Immunology 2024Quote: ... and M-MuLV Reverse Transcriptase (New England Biolabs, Ontario, Canada) as described elsewhere(20).
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bioRxiv - Immunology 2024Quote: ... 1X M-MuLV Reverse Transcriptase Reaction Buffer (New England Biolabs), 5mM DTT ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.25μL of M-MuLV Reverse Transcriptase (New England BioLabs, M0253L) and 1.6μL of water were added to the reaction and incubated at 42°C for 1 hour followed by heating at 90°C for 10 min before cooling.
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bioRxiv - Genomics 2022Quote: ... The pellet was resuspended in 1 mL of NEB and centrifuged at 11,000rpm for 20 min on a sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The Nuclei pellet was then resuspended in 1 mL of NEB ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei-containing pellets were resuspended in 1 mL of NEB + 0,25 M sucrose and centrifuged at 20000xg for 20 min on sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The pellet was resuspended in 1 mL of NEB and formaldehyde to a final concentration of 1% ...
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bioRxiv - Microbiology 2021Quote: ... and 10 U/mL of M-MuLV Reverse Transcriptase (NEB, M02553L) was added to each sample and incubated for 1 hr at 42°C ...
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bioRxiv - Biochemistry 2021Quote: ... RNA samples were reverse transcribed using M-MuLV Reverse Transcriptase (NEB) for 1 h at 42 °C ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized using M-MuLV Reverse Transcriptase (NEB) enzyme ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription (RT) was performed with M-MuLV reverse transcriptase (NEB) according to manufacturer instructions with a short oligonucleotide to prevent annealing of the oligonucleotides on RNAs that lost a few nucleotides at the 3’ end during the migration (and would thus run faster in the reverse run ...
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bioRxiv - Biochemistry 2023Quote: ... was coupled to magnetic beads (Dynabeads M-270 Epoxy, NEB 14302D) at the ratio of 10 μg antibody/mg of beads in the presence of 1 M ammonium acetate and 0.1 M sodium phosphate ...
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bioRxiv - Bioengineering 2023Quote: PstI-HF restriction enzyme (NEB cat. no. R3140S/L/T/M)
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bioRxiv - Bioengineering 2023Quote: BamHI-HF restriction enzyme (NEB cat. no. R3136S/L/T/M)
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using M-MuLV Reverse Transcriptase (New England Biolabs) with random hexamer primers (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was prepared using M-MuLV Reverse Transcriptase (New England Biolabs) and oligo(dT)20 primers using the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... 0.75 M NaCl) and 2 μl protein kinase K (NEB, Ipswich, USA) during 2 h at 60 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 0.8 M guanidine HCl) was supplemented with Proteinase K (New England Biolabs), diluted to 8 units mL-1 ...
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bioRxiv - Microbiology 2020Quote: ... and pAAVS1_c- LTatCL[M]) were linearized using 100 units of NsiI (NEB)/20 μg DNA ...
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bioRxiv - Plant Biology 2021Quote: ... AT5G02330 and AT2G13900 cDNA was synthesized using M-MuLV Reverse Transcriptase (NEB) with 100 ng of total RNA at 42ºC for 60 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM dNTPs and 200 U M-MuLV (New England BioLabs, USA) which were treated following manufacturer’s instructions with some modifications ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant M-MuLV reverse transcriptase from the ProtoScript® II kit (NEB) was used to synthesize first strand cDNA from the annealed primer-MS2 RNA mix ...
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bioRxiv - Biochemistry 2023Quote: ... 1 M NaCl and 8 units/ml Proteinase K (New England Biolabs) in 1x PBS] ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was achieved using the M-MuLV Reverse Transcription kit (NEB), according to manufacturer’s guidelines ...
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bioRxiv - Genetics 2023Quote: ... 2 μL 1 M CaCl2 and 5 μL micrococcal nuclease (NEB, #M0247S) were added and chromatin was fragmented into predominantly mono-nucleosomes by incubation at 37°C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... and reverse transcribed with M-MuLV Reverse Transcriptase (New England Biolabs, M0253) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was reverse-transcribed with M-MuLV reverse transcriptase (New England Biolabs, M0253) and random hexomers (ThermoFisher N8080127) ...
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bioRxiv - Immunology 2022Quote: ... Total RNA was reverse transcribed using M-MuLV reverse transcriptase (New England Biolabs) following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2021Quote: ... yielding almost 1.6 M mapping-quality variants (homozygous in NEB, absent from WUS). ddRADseq libraries were de-multiplexed and barcodes removed and variants called using a GATK 4.0 best practices pipeline29 ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA was synthesized using the M-MLV RTase (New England Biolabs Japan) and subjected to the real-time qPCR using StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: ... 8 μL of 5 M NaCL and 1 μL of RNase A (NEB) were added to every 200 μL of eluate and to the WCE samples (diluted to 200 μL with Elution buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.75 µl 0.1 M DTT and 0.3 U/µl T4 Polynucleotide kinase (NEB), and then incubated at 37°C 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... was used for cDNA preparation using M-MuLV Reverse Transcriptase (New England Biolabs). The cDNA sequence of TRB5 was amplified using gene specific Gateway-compatible primers according to the manufacturer’s instructions with primers specified in Supplemental Table 4 ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized from mRNA samples with M-MLV (New England Biolabs, M0253S) primed with random hexamers (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... and reversely transcribed into complementary DNA (cDNA) using M-MuLV reverse transcriptase (NEB.M0253S ...
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bioRxiv - Microbiology 2024Quote: ... cDNA synthesis was performed on viral supernatant using M-MuLV Reverse Transcriptase (NEB) with oligo(dT ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was prepared using M-MuLV-reverse transcriptase per the manufacturer’s instructions (NEB). qPCR was performed using PowerUp SYBR green master mix (Applied Biosystems ...