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Citations for New England Biolabs :
51 - 100 of 225 citations for FMOC L MEORN MTR OH since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and the NEBNext® Ultra II Ligation module (cat# NEB #E7595S/L) were used to process the samples ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The NEBNext® Ultra II FS DNA module (cat# NEB #E7810S/L) and the NEBNext® Ultra II Ligation module (cat# NEB #E7595S/L ...
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bioRxiv - Molecular Biology 2022Quote: ... 2.5 U/μL SplintR Ligase (Cat No. M0375 L, NEB, Beijing, China), 1 U/μL RiboLock RNase Inhibitor (Cat No ...
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bioRxiv - Bioengineering 2024Quote: ... A mastermix containing 2 mmol/L of each dNTP (NEB, Ipswich, MA), 4.5 µg of random hexamers (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: (Optional) OneTaq Quick-Load 2X Master Mix (NEB cat. No. M0486S/L), for genotyping only
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bioRxiv - Bioengineering 2023Quote: NEBuilder HiFi DNA Assembly Master Mix (NEB cat. no. E2621S/L/X)
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bioRxiv - Bioengineering 2023Quote: Q5 Hot Start High-Fidelity DNA Polymerase (NEB, cat. no. M0493S/L)
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bioRxiv - Immunology 2022Quote: ... and L DNA was generated with HindIII-HF (New England Biolabs, USA). A CIMac pDNA 0.3 mL weak anion-exchange analytical column (Sartorius ...
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bioRxiv - Molecular Biology 2021Quote: BMDMs were lysed in l-PPase reaction buffer (New England BioLabs, Ipswich, MA) supplemented with 0.4% NP-40 and EDTA-free protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.2 μ L of RNase Inhibitor (Catalog No. M0314, New England BioLabs Inc.), and 8 μ L of nuclease-free water ...
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bioRxiv - Microbiology 2020Quote: ... or anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) were used as the secondary antibodies.
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bioRxiv - Biophysics 2023Quote: ... The left lane (“L”) has a DNA size ladder (1 kb+, NE Biolabs). The black arrow in the left lane (“P” ...
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bioRxiv - Microbiology 2024Quote: ... The GA was done according to the manufacturer protocol (NEB E2611S/L, E5510S).
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bioRxiv - Biochemistry 2021Quote: ... Enzymatic deglycosylation of NTD was carried out by adding 2.5 L Endo Hf (NEB) per 20 μg of NTD and incubating for 24 hrs at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Deletion mutants of hnRNP L and SETD2 were constructed by PCR (Phusion polymerase, NEB) using full-length hnRNP L and SETD2 ...
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bioRxiv - Cell Biology 2023Quote: ... RNAs were selected using the NEB Next Poly A+ Isolation Kit (NEB #E7490S/L). Poly A+ fractions were eluted ...
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bioRxiv - Microbiology 2022Quote: ... purified using the Monarch RNA Cleanup Kit (T2050S/L, New England Biolabs, Ipswich, Massachusetts), reannealed by heating to 94°C and slowly cooling to room temperature over 50 min ...
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bioRxiv - Genomics 2022Quote: ... The NEBNext® Ultra DNA Library Prep kit for Illumina (cat# NEB #E7370S/L) was used to process the DNA samples ...
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bioRxiv - Molecular Biology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #6442S/L). Sequencing of the libraries was performed with Illumina NovaSeq 6000 SP flow cell using paired-end 150-bp format (300 cycles = 2×150bp ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was selected using the NEB Next Poly A+ Isolation Kit (NEB #E7490S/L). Poly A+ fractions were eluted ...
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bioRxiv - Molecular Biology 2022Quote: The NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB #E7370S/L) was used to prepare DNA sequencing libraries ...
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bioRxiv - Biochemistry 2021Quote: ... This was added to 5 ml (per 2 L of culture) amylose resin (New England Biolabs) equilibrated in lysis buffer and left on a tube roller shaker at 4 °C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA was then digested using Dpn II (375 U DpnII (NEB, R0543 T/M/L) in 1× Dpn II buffer (NEB ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using the NEBNext Ultra II kit (New England Biolabs, cat. no. E7645S/L). Index primers set 1 and 2 from the NEBNext Multiplex Oligos for Illumina kit (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... We performed the optimized TMS with enzymatic shearing using 4uL of NEBNext UltraShear (NEB #M7634S/L) for 10 or 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µL phosphorylation mix (1µL 100mM sense oligo, 1µL 100mM anti-sense oligo, 0.5µL 25mM ATP (BioLabs #P0756S), 1µL 10X PNK T4 Buffer (BioLabs #B0201S) ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...
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bioRxiv - Plant Biology 2022Quote: ... The samples were pooled by row into 8-strip tubes and excess primers were digested with the addition of 4.6 µL exonuclease I mix (2.5 µL of 10X Exonuclease I Buffer, 2.1 µL Exonuclease I; New England Biolabs) then incubated at 37□ for 20 min ...
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bioRxiv - Immunology 2021Quote: ... 3.6 μL of exonuclease reaction solution (2.52 μL sterile nuclease-free water, 0.36 μL Exo reaction buffer, and 0.72 μL Exo-I, New England BioLabs) was then added to remove unincorporated primers (37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1, 1μL of 1mM dATP, 5U Klenow exo (NEB M0212S)) to the 41μL of DNA sample from the previous step ...
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bioRxiv - Genomics 2021Quote: ... dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1, 5μL of 1mM dATP, 3U Klenow exo (NEB M0212S)) to the 41μL of beads with bound DNA from the previous step ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM SARS_139 and SARS_140 primers were mixed with 1 μl T4 Polynucleotide kinase (PNK; NEB) in 1x PNK reaction buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 ⎧M of strain specific primers and 0.02U/⎧L of Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in a total reaction volume of 25 ⎧L ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (#7530 L, NEB, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... 1μl XhoI digestion mix consisting of 5U XhoI restriction enzyme and 1 x NEB buffer 2.1 (New England Biolabs), and 10 ng genomic DNA for iciHHV-6B samples or 200 ng DNA for non-iciHHV-6B samples ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... then washed once and resuspended in 500 l 1.2x EcoRI or DpnII restriction enzyme buffer from New England Biolabs (NEB). An aliquot of 50 l of nuclear solution (∼100mg embryos ...
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bioRxiv - Microbiology 2021Quote: ... 40 μL purified supernatant were combined with 2,500 units recombinant glycerol-free PNGase F and Glycobuffer 2 (New England Biolabs) following the manufacturer’s protocol for non-denaturing digestion and incubated at 37°C for 5 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 μl of 10x RNAse H Buffer (500 mM Tris-HCl, 750mM KCl, 30mM MgCl2, 100mM DTT, pH 8.3; NEB) and 1 μl of 5 units/µl RNase H (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in 48 μL elution buffer (50 mM Tris pH 8, 1 mM EDTA, 1% SDS) and 1.6 U Proteinase K (NEB), incubated at 55 °C for one hour and then 65 °C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Remodeling reactions were started by adding 10 μL SGD chromatin corresponding to ~ 1 μg DNA assembled into nucleosomes and terminated by adding 0.8 Units apyrase (NEB) followed by incubation at 30 °C for 30 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... 11.25 μ L OneTaq Hot Start 2X Master Mix with Standard Buffer (Catalog No. M0484, New England BioLabs Inc.), 1.25 μ L Chai Green Dye 20X (Catalog No ...
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bioRxiv - Genomics 2020Quote: ... 200–300 ng/µL of high molecular weight DNA underwent single-strand nicking with 10 units of Nt.BspQI nickase (New England BioLabs). Nicked sites were repaired with fluorophore-labeled nucleotide to restore strand integrity.
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bioRxiv - Molecular Biology 2021Quote: ... NEBNext Ultra II DNA Library Prep Kit for Illumina was used to make the DNA libraries (NEB, #E7645S/L), which were sequenced on the Illumina NovaSeq (PE-150 ...
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bioRxiv - Molecular Biology 2023Quote: ... in a 10 μL reaction in buffer (50 mM Tris-HCl pH 7.5, 1 mM DTT, 1 U/μL RNase Inhibitor (NEB)) and incubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were re-suspended in 100µl PNK-mix (70mM Tris-HCl pH6.5, 10mM MgCl2, 1mM DTT, 100U RNasin, 1U TurboDNase, 25U Polynucleotide-Kinase (NEB)) and incubated for 20min at 37°C in a thermomixer with shaking (1200 x rpm) ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.75 μl of second strand mix (0.5 μl of mRNA second strand synthesis buffer and 0.25 μL of mRNA second strand synthesis enzyme, New England Biolabs) were added to each well ...
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bioRxiv - Molecular Biology 2024Quote: ... an equal amount of DNA (8μg) was treated with 8□l of RNase H in 1x RNase H buffer (NEB) for 5 hours at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.5 µL of 10X GlycoBuffer 3 (Cat.#B1720S; New England Biolabs) and 2.5 µL of Endo Hf in a total reaction volume of 25 µL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.6 µL of 10X GlycoBuffer 2 (Cat.#B3704S; New England Biolabs), 2.6 µL of 10% NP-40 (Cat.#B2704S ...