Labshake search
Citations for New England Biolabs :
9501 - 9547 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Isolated mRNA material was used to prepare the libraries using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, Cat No. E7760S) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Low quality DNA were then reconcentred with AMPure XP beads and libraries were constructed according to the NEBNext® Ultra II DNA Library Prep for Illumina® kit recommendations (New England Biolabs, Ipswich, MA, USA). Each library was individually labeled and subjected to 12 PCR cycles for final enrichment and pooled in equal quantities ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75 ...
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bioRxiv - Developmental Biology 2023Quote: ... The NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) were used based on the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription reactions were then used as input material for NEBNext® Ultra™ II DNA library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), using NEBNext Multiplex Oligos for Illumina® (Unique Dual index UMI Adaptors DNA Set 1 ...
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bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit, New England Biolabs, GmbH, Frankfurt am Main, Germany). PCRs were performed using the Q5 polymerase (Q5 Hot Start High-Fidelity ...
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bioRxiv - Immunology 2022Quote: ... Libraries for bulk RNA sequencing were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra™ II Directional RNA Library Prep Kit for Illumina (New England BioLabs, cat. #E7490 and #E7760). The input RNA and the resulting libraries were analyzed with Agilent 4200 Tapestation for quality assessment ...
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bioRxiv - Immunology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Paired-end libraries of 2 x 150 bp fragments with an insert size of 330 bp were prepared with NEBNext® Ultra™ II DNA Library Prep Kits (New England Biolabs, Ipswich, MA, USA), and sequencing was performed on a HiSeq4000 Illumina sequencer (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... The constructs M1 and M2 were used as templates to generate higher-order mutants by site-directed mutagenesis PCR (NEB, Q5 Site-Directed Mutagenesis Kit) using the primers listed in Table S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded RNA-Seq libraries were prepared with 200 ng of total RNA with RIN > 8 using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Poly A+ RNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation and first and second cDNA strand synthesis ...
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bioRxiv - Immunology 2023Quote: ... dual unique indexed libraries were made using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Manufacturer protocol was modified by diluting adapter 1:30 and using 3μL of this dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing of RNA samples was performed by Azenta Life Sciences (Burlington, Massachusetts, US) with NEBNext®Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, Massachusetts, US) for library preparation.
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bioRxiv - Genomics 2023Quote: ... 1 µg of the resulting DNA was used for library preparation using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB cat. E7103). Library PCRs were supplemented with 1× EvaGreen (Jena Bioscience cat ...
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bioRxiv - Biophysics 2023Quote: ... The mutation was introduced with an oligonucleotide designed to alter codon Arg90 (CGC) to Ser (AGC) using a Q5TM site-directed mutagenesis kit (#EO552, #EO554S, New England BioLabs GmbH, Frankfurt am Main, Germany). The successful introduction of mutations was confirmed by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting mRNA material was used to prepare the libraries with the use of NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, Cat No. E7760S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... All remaining steps for library construction were used according to the NEBNext® UltraTM II Non-Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). Final libraries quantity was assessed by Qubit 2.0 (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Library preparation for gene expression analysis was performed with the NEBNext® Poly(A) mRNA magnetic isolation module and NEBNext® UltraTM II Directional mRNA kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions with 1μg total RNA as input material ...
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bioRxiv - Biochemistry 2024Quote: ... A His-Tag encoding sequence was added to the C-terminus of RnfG by using oligonucleotides 5 and 6 via site-directed mutagenesis (Q5® Site-Directed Mutagenesis Kit, NEB, Frankfurt am Main, Germany). The resulting plasmid was checked by sequencing analysis (Microsynth Seqlab ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were sent to the Penn State Genomics Core Facility for library preparation using NEBNext® UltraTM II DNA Library Prep kits for Illumina® with NEBNext® Multiplex Oligos (New England Biolabs, NEB: Ipswich, MA). Each library was prepared from 500ng DNA ...
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bioRxiv - Genomics 2024Quote: Illumina sequencing libraries for good quality samples (total DNA amount > 20 ng) were prepared with the standard NEBNext®Ultra™ II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA), while double stranded libraries for the samples extracted in the ancient DNA laboratory were prepared following the Meyer and Kircher protocol67 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and sequenced it as a 400- 600bp insert Illumina® library using the NEBNext Ultra II® Library Preparation kit (New England Biolabs® [NEB], Ipswich, MA USA) on an Illumina® NovaSeq lane (Novogene® ...
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bioRxiv - Genomics 2022Quote: ... Capture and removal of CpG methylated host DNA from samples were carried out using the NEBNext® Microbiome DNA Enrichment Kit (NEB, New England Biolabs, Ipswich, MA), following the manufacturer’s recommendations with modifications (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: 1000 ng per sample was then processed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (cat # E7760L; New England Biolabs, Ipswich, USA; protocol v. v3.1_5/20) including PolyA selection ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent pENTR clones were made by excising the original insert and replacing with new candidate enhancer fragments via Gibson Assembly (NEB HiFi Assembly Kit, New England Biolabs). The candidate enhancer fragments were shuttled to the transgenic vector pBPGUw [105] upstream of a minimal synthetic promoter sequence driving Gal4 using standard Gateway cloning techniques (LR Clonase ...
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bioRxiv - Cell Biology 2021Quote: ... 80ng of genomic DNA were fragmented to around 170bp followed by End Pair and A tailing with the End Pair mix (NEB Next Ultra DNA library preparation kit). After ligation of truncated TruSeq adapters with the NEB Next Ultra Ligation Module ...
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bioRxiv - Plant Biology 2022Quote: ... Paired end libraries for Illumina sequencing were constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Inc, MA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... All libraries were constructed according to the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). The libraries were quantified by Qubit 2.0 and their quality was assessed by TapeStation D1000 ScreenTape (Agilent Technologies Inc. ...
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bioRxiv - Molecular Biology 2022Quote: ... then quantified with the NEBNext® Library Quant Kit for Illumina® using qPCR on a StepOne Plus real-time PCR platform (New England Biolabs® Inc., MA, USA). Following qPCR ...
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bioRxiv - Neuroscience 2022Quote: ... at −80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Immunology 2021Quote: ... Master mix preparation and cycling conditions were realized with Luna® Universal qPCR Master Mix kit (cat. n° M3003E, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... One microgram of mRNA was used as a template for first-strand cDNA synthesis using NEBNext® Poly(A) kit (NEB #E7490, New England Biolabs, Inc.) and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760 ...
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bioRxiv - Microbiology 2021Quote: Metagenome sequencing libraries were prepared with the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs GmbH, Frankfurt am Main, Germany) and sequenced on an Illumina NextSeq500 sequencer using 2 × 150 bp ...
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bioRxiv - Microbiology 2020Quote: Libraries were prepared for RNA sequencing according to the instructions of the NEBNextl® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New Englands Biolabs, Ipswich, MA, USA), with the following exception ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA; input 60 ng). Manufacturer’s instructions were followed except for Step 4 ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA library preparation was performed using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (# E7645S/L, New England BioLabs, Frankfurt am Main, Germany) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: DNA fragments recovered from S1 nuclease mapping and ChIP experiments were used to construct Illumina sequencing libraries using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, catalog No. E7645L) NEBNext® Multiplex Oligos for Illumina® Index Primer sets 1 ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The RNA sequencing library was generated from 100 ng RNA using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (#57760S and #57765S, New England Biolabs, Frankfurt am Main, Germany) with the NEBNext® Poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... at -80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Microbiology 2023Quote: ... we prepped samples with the NEBNext® FFPE DNA Repair Mix and NEB-Next® Ultra™ II End Repair/dA-Tailing kits (New England Biolabs, Ipswich, MA, USA) and performed DNA cleanup Ampure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were sent to the Penn State Genomics Core Facility for library preparation using NEBNext® UltraTM II DNA Library Prep kits for Illumina® with NEBNext® Multiplex Oligos (New England Biolabs, NEB: Ipswich, MA). Each library was prepared from 500ng DNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and sequenced it as a 400- 600bp insert Illumina® library using the NEBNext Ultra II® Library Preparation kit (New England Biolabs® [NEB], Ipswich, MA USA) on an Illumina® NovaSeq lane (Novogene® ...
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bioRxiv - Microbiology 2021Quote: The libraries for the Illumina sequencing were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England BioLabs GmbH, Frankfurt am Main, Germany) according to the protocol for large fragment sizes >550 bp but with a minimal fragmentation time of only 30 s ...
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bioRxiv - Genomics 2022Quote: ... Strand-specific RNA sequencing library and small RNA sequencing libraries were generated respectively using NEBNext® Ultra™ RNA Library Prep Kit and NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, Ipswich, MA, USA). After the cDNA synthesis and PCR amplification ...
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bioRxiv - Genomics 2024Quote: NEB VarSkip amplicon libraries were prepared according to the NEBNext® ARTIC SARS- CoV-2 FS Library Prep Kit Workflow for Illumina® (NEB, Cat No. E7658, Ipswich, MA).
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bioRxiv - Plant Biology 2021Quote: A sequencing library for the flower RNA sample was prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs®, Inc., Ipswich, MA, USA) and barcoded using index primers from the NEBNext® Multiplex Oligos for Illumina® (New England BioLabs® ...