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Citations for New England Biolabs :
9451 - 9500 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2023Quote: ... The recombinant clones containing these constructs were then grown overnight in LB liquid media containing 25 μg/ml of Zeocin™ antibiotic and plasmid DNA isolation was isolated using the Monarch® Plasmid Miniprep Kit (New England Biolabs, Whitby, ON). Base-pair accuracy and orientation of the receptor constructs were confirmed by Sanger sequencing (Center for Applied ...
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bioRxiv - Bioengineering 2023Quote: ... RNA synthesis was conducted via in vitro transcription using a HiScribe™ T7 high yield RNA synthesis kit (New England BioLabs, NEB, Ipswich, MA). After DNase I (RNase-free ...
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bioRxiv - Plant Biology 2023Quote: ... High quality of RNA was used to prepare RNA-Seq libraries with NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England BioLabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ng total RNA were used as input for ribosomal RNA (rRNA) depletion using the NEBNext rRNA depletion kit (New England BioLabs inc., product code: E6350) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: For library construction the ChIP purified DNA was used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645L) with modifications of the manufacturer instruction as we assembled half volume reactions ...
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were obtained using the NEB-Next® UltraTM II RNA library prep kit for Illumina® (E7770S, New England Biolabs, Ipswich, MA). The isolated mRNA was fragmented ...
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bioRxiv - Microbiology 2023Quote: ... Point mutations to generate the mutant variants were introduced using the Q5® Site-Directed Mutagenesis Kit purchased from New England Biolabs (NEB, Ipswich, MA, USA). Primers were acquired from Sigma Aldrich (St ...
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bioRxiv - Microbiology 2023Quote: ... The resultant fragments were cloned into plasmid pKNG101 by using the NEBuilder® HiFi DNA Assembly Cloning kit (New England Biolabs, Ipswich, MA, USA) (33) ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries using 500 ng of good-quality RNA (RIN ˃7and a NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA, #E7760L) at the Genome Québec core facility and paired-end sequenced on a NovaSeq6000 S4 sequencer ...
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bioRxiv - Microbiology 2023Quote: ... the products were then used to construct sequencing libraries using the NEB Next® Ultra II™ DNA Library Prep Kit for Illumina® (New England Biolabs, USA) following the manufacturer’s recommendations.
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bioRxiv - Synthetic Biology 2023Quote: ... Site directed mutagenesis was then performed for the promoter region of iLight repressor through using NEB Q5® Site-Directed Mutagenesis Kit with non-overlapping primers or NEBuilder® HiFi DNA Assembly (New England BioLabs, MA, USA) with overlapping primers ...
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bioRxiv - Bioengineering 2023Quote: ACE2 plasmids with different cytoplasmic tail lengths were generated using the Q5® Site-Directed Mutagenesis (SDM) Kit (New England BioLabs, Ipswich, MA, USA) according to manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with Unique Dual Index UMI Adapters RNA Set1 (NEB, Franklin Lake, NJ, USA) were used in the succeeding steps according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Cat. No. E7645) and the NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Cat ...
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bioRxiv - Plant Biology 2024Quote: Arabidopsis thaliana RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Preparation Kit (New England BioLabs, MA, USA). Five hundred ng of total RNA was taken for mRNA isolation ...
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bioRxiv - Microbiology 2024Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (96) (New England BioLabs, Frankfurt am Main, Germany). To assess quality and size of the libraries samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies) ...
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bioRxiv - Genomics 2024Quote: ... and polyA mRNA sequencing libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat# E7760L and E7765L) per the manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: ARTIC V4 amplicon libraries were prepared according to the NEBNext® ARTIC SARS- CoV-2 Library Prep Kit Workflow for Illumina® (Discontinued, New England BioLabs, Ipswich, MA). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... RNA concentration and quality were evaluated using NanoDrop 2000 Spectrophotometer (Thermo Fischer Scientific) and reverse-transcribed to cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, Cat no. E3010) with random hexamers and oligo dT ...
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bioRxiv - Cancer Biology 2024Quote: ... iScript™ cDNA Synthesis Kit (Bio Rad, Cat. No. 1708891) was used for cDNA preparation and Luna® Universal qPCR Master Mix (NEB, Cat. No. M3003L) was used for subsequent qPCR ...
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bioRxiv - Neuroscience 2024Quote: High molecular weight (HMW) DNA was extracted from isolated cells using the Monarch HMW DNA extraction kit (New England Biolabs, Ipswich, MA, USA, #T3050L) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested by scraping and total RNA was isolated using the Monarch Total RNA Miniprep kit (T2010S, New England Biolabs Inc., Ipswich, MA, USA) according to the supplierś instructions ...
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bioRxiv - Zoology 2024Quote: ... and post-aestivation stages) were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module followed by NEBNext kit (E7490, NEB, Ipswich, MA, USA). The quality and quantity of the libraries were checked on an Agilent 2100 Bioanalyzer using the Agilent DNF-935 Reagent Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: cDNA was synthesized from the total RNA samples using either the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA), following the standard protocol with Random Primer Mix ...
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bioRxiv - Genomics 2024Quote: Adult worm Illumina sequencing libraries were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Cat: E7805L) and DNA concentrations measured with the dsDNA High Sensitivity Assay kit on a Qubit 4 Fluorometer (both Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic libraries were prepared using the NEBNext® Ultra TM II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, United States) using reagents at half volumes following Hale et al ...
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bioRxiv - Molecular Biology 2019Quote: The purified PCR products were used as templates for in vitro transcription of antisense and sense ssRNA by using the HiScribe™ T7 In Vitro Transcription Kit (NEB #E2030S, New England BioLabs Inc, Ipswich, Massachusetts, USA). Finally ...
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bioRxiv - Systems Biology 2020Quote: ... mRNA-seq libraries were prepared for sequencing using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England BioLabs, Ipswich, MA, USA) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... RNA libraries were constructed according to standard protocol using NEB Next® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA). Ribosomal RNA depletion was carried out using Ribo-zero rRNA Removal Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... 400ng of RNA was used to generate sequencing libraries using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England BioLabs, MA, USA) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Equal amount of total RNA in a maximal volume of 6 µ L is used for cDNA synthesis using NEB PhotoScript® First Strand cDNA Synthesis Kit (Catalog # E6300L, NEB Inc, Ipswitch, MA) as instructed in the manufacturer standard protocol ...
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bioRxiv - Neuroscience 2021Quote: A total amount of 0.4 ug of RNA was used for cDNA library construction at Novogene using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (cat NEB #E7775, New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA fragments corresponding to the full-length ORFs of the candidate genes were then amplified via PCR with Q5® High-Fidelity Polymerase PCR kit (NEB, Frankfurt am Main, Germany) using gene-specific gateway primers (Supplementary Table S9) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The cDNA libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs, Ipswich, MA, USA) and sequenced on two lanes (two chips ...
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bioRxiv - Genomics 2022Quote: ... Libraries were constructed at the McGill Genome Center using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) following manufacturer’s recommendations and sequenced by Illumina HiSeq 2×150 cycles run (Illumina Inc. ...
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bioRxiv - Genomics 2020Quote: ... double stranded cDNA synthesis was performed from DNase pretreated total nucleic acid extracts using the NEBNext® Ultra™ II RNA First Strand Synthesis Module and NEBNext® Ultra™ II Non-Directional RNA Second Strand kit (New England Biolabs, MA, USA). The enrichment was based on a hybridization step with a respiratory viruses biotinylated adjacent oligoprobes panel (16 ...
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bioRxiv - Molecular Biology 2019Quote: ... 400ng of RNA was used to generate sequencing libraries using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England BioLabs, MA, USA) following manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2021Quote: ... The remaining mRNA was used for stranded RNA-seq library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... was used for poly A selected RNA-seq library preparation using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (mRNA) (New England Biolabs Cat#E7530S). Biological replicates were individually barcoded ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed using 1ug DNA-free total RNA with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA. NEB#E7770). Poly (A ...
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bioRxiv - Genetics 2020Quote: ... 5 ng of fragmented DNA were then used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), as per manufacturer instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... mRNA Magnetic Isolation Module in combination with the NEBNext® Ultra™ II Directional RNA Library Prep Kit and indexed with NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) (New England Biolabs, Ipswich, Massachusetts, USA). Fragmentation was carried out using incubation conditions recommended by the manufacturer for samples with a RIN ≥7 to produce RNA sizes ≥ 300bp (94°C for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The IL-2 signal sequence that was present in the original mCherry-progranulin plasmid was later replaced with progranulin signal sequence by site-directed mutagenesis (NEB Q5 site directed mutagenesis kit). For cloning of Prosaposin-RUSH (Str-KDEL_Prosaposin-SBP-mCherry) ...
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bioRxiv - Genomics 2022Quote: ... The RNA samples and standards were amplified using the Luna® Universal Probe One-Step RT-PCR Kit (E3006, BioLabs®Inc, Ipswich, Massachusetts, USA), with 0.6 mM primer and 0.2 mM probe concentrations (Table S4) ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcriptase and RT-qPCR reactions were conducted using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, United States). The reaction was performed with 50 ng of total RNA in a total volume of 20 µL ...
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bioRxiv - Biophysics 2022Quote: Site-specific mutagenesis was performed with the Q5® Site-Directed Mutagenesis Kit (based on PCR-amplification) according to the manufacturer’s protocol (New England Biolabs GmbH, Frankfurt am Main, Germany). Oligonucleotides were ordered from metabion GmbH (Planegg/Steinkirchen ...
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bioRxiv - Molecular Biology 2023Quote: ... For the library preparation of the pools the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich (MA) – United States of America ...
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bioRxiv - Neuroscience 2023Quote: ... Both input samples without IP and m1A input samples were used for library construction using an NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, Inc., MA, USA). Eluted RNA fragments were converted to cDNA and sequenced or treated to induce partial m1A to m6A conversion before cDNA synthesis ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75 ...
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bioRxiv - Neuroscience 2022Quote: ... Polyadenylated RNA was captured from 50 ng of total RNA per sample and libraries were prepared using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Paired-end sequencing was done at the Vanderbilt VANTAGE core laboratory (Vanderbilt University ...