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Citations for New England Biolabs :
901 - 950 of 1251 citations for Recombinant Mouse Angiotensinogen serpin peptidase inhibitor clade A member 8 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 50 μg/ml BSA) (12) and 40 U Murine RNase Inhibitor (NEB). Aliquots were withdrawn after 3 and 30 minutes at 25 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1 µL of murine RNase inhibitor (40,000 units/mL, New England Biolabs), 0.125 µL of T7 polymerase ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 units/µl of murine RNase inhibitor (New England Biolabs, M0314L). In minigene experiments ...
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bioRxiv - Genomics 2023Quote: ... 0.5 U/μL murine RNase inhibitor (New England Biolabs, Cat. No M0314S), and 2U/μL Maxima H-minus RT (Thermo Scientific ...
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bioRxiv - Genetics 2023Quote: ... 2 μL RNase inhibitor (M0314; New England Biolabs Inc.; Ipswich MA, USA) and 2 μL MNase (M0247 ...
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bioRxiv - Neuroscience 2024Quote: ... A mixture of 100 ml of RNAse Inhibitor (New England BioLabs M0314L) and 250 ml of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Neuroscience 2024Quote: ... The Stereo-seq capture chip was cleaned with RNase inhibitor (NEB, M0314L), enriched with NF-H2O ...
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bioRxiv - Microbiology 2021Quote: ... together with the Bakt_314F (CCTACGGGNGGCWGCAG) and Bakt_805R (GACTACGVGGGTATCTAATCC)38 PCR primer pair with an individual 8 bp barcode adapter (based on the NEB Multiplex Oligos for Illumina ...
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bioRxiv - Bioengineering 2020Quote: ... Constriction was performed by diluting a library to approximately 8 fM (~5,000 molecules/μl) in salmon sperm DNA (1 ng/μl, New England Biolabs) in low-binding tubes (LoBind ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 1:8 dilution Cas9 protein (New England Biolabs, cat. M0386M; diluted in buffer B, New England Biolabs, cat. B802S) and 1:40 dilution of phenol red (Sigma Aldrich) ...
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bioRxiv - Evolutionary Biology 2021Quote: DNA was isolated from single fins by placing them in lysis buffer (10mM Tris pH 8, 100mM NaCl, 10 mM EDTA, 0.5% SDS) with Proteinase K (333µg/mL, NEB P8107S) at 55°C for between four hours and overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Two separate 8-µl aliquots of beads derived from each strain were treated with 1 µl lambda phosphatase (NEB) in 10 µl reactions containing ...
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bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in 48 μL elution buffer (50 mM Tris pH 8, 1 mM EDTA, 1% SDS) and 1.6 U Proteinase K (NEB), incubated at 55 °C for one hour and then 65 °C overnight ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 8 cDNA libraries were prepared with an NEBNext Ultra II RNA Library Prep kit (NEB, cat:E7770S) and sequenced with an Illumina HiSeq4000 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Barcodes (4-8 bp) and common adapters were ligated with 400 U T4 DNA ligase (New England Biolabs Inc.) at 22 C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... an equal amount of DNA (8μg) was treated with 8□l of RNase H in 1x RNase H buffer (NEB) for 5 hours at 37°C ...
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bioRxiv - Genomics 2023Quote: ... ATAC-seq libraries were prepared by ∼8 cycles of PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB) and primers containing Illumina barcodes (Buenrostro et al ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 μl of a mix preheated to 37°C and containing 2.5 U of RNase H (New England Biolabs), 2.5× RNase H reaction buffer (1× buffer ...
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bioRxiv - Genomics 2023Quote: ... Libraries were amplified by PCR for a total of 8 cycles in 100µL reactions with Phusion polymerase (New England Biolabs). No PAGE purification was performed to ensure that our libraries were not biased against short nascent RNA insertions.
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bioRxiv - Microbiology 2023Quote: ... by mixing 2 ul 1:10.000 diluted library with 8 ul Quant mastermix with added primers (New England Biolabs). Due to low initial concentration of adapter ligated product we made triplicates of the library ...
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bioRxiv - Biochemistry 2023Quote: ... Snap-cooled RNAs were diluted to 8 nM in 2x RNA master mix (0.2 mg/mL bovine serum albumin (molecular biology grade, NEB), 0.2 mg/mL yeast tRNA ...
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bioRxiv - Biochemistry 2024Quote: ... then diluted to 8 nM concentration in 2x RNA master mix (0.2 mg/mL bovine serum albumin (molecular biology grade, NEB), 0.2 mg/mL yeast tRNA ...
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bioRxiv - Genetics 2021Quote: ... 5 μg of MBP-Rif2 or MBP-Rif2-min (see recombinant protein preparation) was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, 50 μl per reaction). The resin with the immobilized MBP-Rif2 variants was washed 5 times with 1 ml wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... 1 x Protease Inhibitor Cocktail) with appropriate amount of MNase (New England Biolabs) and incubated at 37 °C for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 U/µL RNase inhibitor (New England Biolabs, Ipswich, MA, USA). Released RNA from permeabilized tissues was captured using DNB probes and reverse-transcribed overnight at 42°C using in-house SuperScript II (10 U/µL reverse transcriptase) ...
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bioRxiv - Microbiology 2022Quote: ... All reactions were supplemented with 0.8 U/µL RNase Inhibitor Murine (NEB, M0314S). Purified CapRelSJ46 protein was used at a final concentration of 250 nM ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.2 μ L of RNase Inhibitor (Catalog No. M0314, New England BioLabs Inc.), and 8 μ L of nuclease-free water ...
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bioRxiv - Bioengineering 2022Quote: ... 120 nM fluorescent beacon and 1 U.μL-1 murine RNase Inhibitor (NEB M0314) in the reaction buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... with the addition of 20 U of Murine Rnase Inhibitor (New England Biolabs). qPCR reactions were run in 12 μl with PowerUpTM SYBRTM Green Master Mix (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.4 U/μL Murine RNAse Inhibitor (Catalog No. M0314S, New England BioLabs). Reactions were incubated for 8 hours at 37°C in a thermocycler ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg/mL cycloheximide) supplemented with 0.1 U/µL murine RNase Inhibitor (NEB), and passed through a G25 needle 25 times ...
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bioRxiv - Biophysics 2023Quote: ... Samples used in RNA titrations additionally contained a Murine RNAse inhibitor (NE Biolabs) at a concentration of 1 unit/μL ...
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bioRxiv - Molecular Biology 2024Quote: ... 1x protease inhibitors) and incubated with 0.3 U/μl CIP (New England BioLabs) for 1 hour at room temperature prior to loading onto a gel.
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bioRxiv - Cell Biology 2024Quote: ... and phosphatase inhibitors sodium orthovanadate (New England Biolabs, Ipswich, MA, USA; item # P0758) and sodium fluoride (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... 2U/ul RNAse inhibitor and supplemented with ribonucleoside vanadyl complex (Concentration, NEB S1402S), then incubated for 30 minutes with secondary antibodies at RT ...
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bioRxiv - Microbiology 2024Quote: ... All reactions were supplemented with 0.8 U/µL RNase Inhibitor Murine (NEB, M0314S). Purified His6-MBP-tagged CapRelSJ46 protein was added to the reaction at a final concentration of 500 nM ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-MBP (New England Biolabs, RRID:AB_ 1559738), used at 1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse anti-PPARα (1:25, Arigo Biolabs ARG55240). Alexa Fluor conjugated secondary antibodies (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The protein was buffer exchanged into enterokinase cleavage buffer (20 mM Tris pH 8, 50 mM NaCl, 2 mM CaCl2) and cleaved using bovine enterokinase (EK, NEB) at 16U/mg protein for 4 hrs ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples with RNA Integrity Number > 8 were used for library preparation following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). Library construction and sequences was carried out at the Biomedical Research Centre Sequencing Facility (University of British Columbia ...
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bioRxiv - Molecular Biology 2020Quote: ... PEARL extracts from cultured cells were treated with either DNase I (1 unit per every 8 μL of PEARL extract, New England BioLabs) or with RNase A (0.1 mg per every 8 μL of PEARL extract ...
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bioRxiv - Molecular Biology 2021Quote: ... after incubating the samples with 2 μl of RNase A (Thermo) at 37 °C for 2h and then with 8 μl of proteinase K (NEB) at 55 °C for 4h ...
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bioRxiv - Cell Biology 2022Quote: ... In-house adapters containing 8-nucleotide unique molecular identifier (UMI) sequences were ligated onto the DNA fragments with T4 DNA ligase (NEB). Excess adapters were removed by AMPure XP beads (Agencourt ...
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bioRxiv - Genomics 2019Quote: The circularized DNA was split into 8 50 ul rolling circle amplification (RCA) reactions (5 ul 10x Phi29 buffer (NEB), 2.5 ul 10 mM dNTPs (NEB) ...
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bioRxiv - Genomics 2019Quote: ... We quantified DNA using a Qubit dsDNA BR assay and performed a debranching treatment on 8-10 µg of DNA in 100 µL reactions containing 50 Units of endonuclease T7E1 (New England Biolabs) in 1X NEB buffer 2 ...
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bioRxiv - Genetics 2019Quote: The mutations comprising the pol30-8 allele (R61A, D63A) were introduced into pEAA578 (POL30∷KANMX) by Q5 site-directed mutagenesis (New England Biolabs) to create the single step integrating vector pEAA633 (pol30-8∷KANMX) ...
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bioRxiv - Microbiology 2021Quote: ... and Bakt_805R (GACTACGVGGGTATCTAATCC)38 PCR primer pair with an individual 8 bp barcode adapter (based on the NEB Multiplex Oligos for Illumina, New England Biolabs) attached to the forward primer and the reverse primer ...
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bioRxiv - Microbiology 2021Quote: ... the codon optimized RBD was PCR amplified and cloned into the bicistronic pDZ rescue plasmid system for A/Puerto Rico/8/1934 using the NEBuider HiFi DNA assembly kit (NEB). Specifically ...
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bioRxiv - Molecular Biology 2020Quote: ... The converted RNA was fragmented into 150 - 200 nt fragments by incubation at 94 °C for 8 minutes in fragmentation buffer (NEB). The fragmented RNA was then used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit ...
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bioRxiv - Molecular Biology 2020Quote: ... 4.8 mM MgCl2 and 1x reaction buffer for T7 RNAP and 62.5 units of T7 RNAP (New England BioLabs, NEB). The mixture was incubated for 2 h at 37°C.