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Citations for New England Biolabs :
851 - 900 of 1251 citations for Recombinant Mouse Angiotensinogen serpin peptidase inhibitor clade A member 8 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... supplemented with 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA) for 1 h at room temperature to allow binding of cognate proteins ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... supplemented with 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA) for 1 h at room temperature to allow binding of cognate proteins ...
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bioRxiv - Neuroscience 2020Quote: ... and sorted directly into lysis buffer containing RNAse inhibitor (NEB E6420). cDNA libraries were made from RNA using NEB’s Next Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB E6420) ...
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bioRxiv - Plant Biology 2020Quote: ... 0.5 mM MgCl2) and 100 U of Murine Rnase Inhibitor (NEB) were added ...
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bioRxiv - Molecular Biology 2019Quote: ... RNase digestion was stopped with 11 µl Murine RNase inhibitor (NEB) and insoluble material removed by centrifugation (15 min ...
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bioRxiv - Microbiology 2019Quote: ... 80 IU/mL Murine RNAse Inhibitor (New England BioLabs Cat# M0314S), 0.1 mg/mL heparin (Sigma H3393-50) ...
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bioRxiv - Genetics 2020Quote: ... and 1 U/μl murine RNase inhibitor (New England BioLabs, #M0314L). The lysate was centrifuged at 2500g for 10 min at 4 °C to remove the pellet debris ...
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bioRxiv - Genomics 2021Quote: ... The sample mix contained 1 μL RNAse Inhibitor (New England Biolabs), 1× ROX Reference Dye (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... and 160 U of RNase inhibitor (New England Biolabs, Ipswich, Massachusetts)) on ice for 10 minutes.
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bioRxiv - Molecular Biology 2024Quote: ... and included the addition of RNase inhibitor (New England BioLabs, UK) in each reaction ...
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bioRxiv - Biochemistry 2023Quote: ... with 1 unit Rnase Inhibitor (New England Biolabs Inc., Massachusetts, USA) and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Biochemistry 2023Quote: ... with 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA) and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Genomics 2023Quote: ... 1 μl SUPERase RNase Inhibitor and 20 μl DdeI (NEB, R0175L). The sample was then incubated at 37 °C for 1 hr and centrifuged at 500x g for 3 minutes ...
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bioRxiv - Genetics 2023Quote: ... Then 0.5 µL of 1 MgCl2 and 2µL RNase inhibitor (NEB) were added to each sample and mixed by vortexing ...
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bioRxiv - Molecular Biology 2024Quote: ... and 11µl Murine RNase Inhibitor (New England BioLabs Inc® M0314L) for 15 mins and were then centrifuged at 20,000 g for 20 mins at 4°C ...
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bioRxiv - Genetics 2021Quote: ... and assembly of the recombinant vector was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA, USA). To generate the double ΔnoxA Δyap1 knockout mutant ...
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bioRxiv - Neuroscience 2022Quote: ... Final concentrations of 5 µM TDP-43-TEV-MBP and the indicated final concentrations of recombinant proSAAS or BSA (NEB BioLabs; B9001S) were achieved by mixing aliquots of a 44 μM stock solution of TDP-43-TEV-MBP with aliquots of stock solutions of either 55 μM proSAAS or 151 μM BSA (both in 5 mM acetic acid) ...
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bioRxiv - Molecular Biology 2022Quote: ... Additional reconstitutions with linker histone were performed by mixing reconstituted 183×12 core arrays with 1 mg/ml recombinant linker histone variant H10 (New England Biolabs, cat.# M2501S) at a molar ratio of 0.7 molecule histone H1 per nucleosome in solution containing 500 mM NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... Histone yields from each purification were determined by using a calibration curve of recombinant H3 and H4 (NEB, cat. #M2503S, #M2504S, respectively). Absolute amounts of histone were determined by densitometry on coomassie-stained bands with Fiji61 for Pristionchus data (conducted at the MPI in Tübingen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each 8-sample pool was then Qbit quantified and amplified using Phusion high-fidelity PCR (New England Biolabs) with a PCR primer with one of three unique barcodes ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was then further purified by a second affinity step over an 8 mL amylose column (NEB), where it was washed with 3 CV of Amylose Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... Final amplification PCR was performed as described before by adding 8 µl of 2 x PCR MM (NEB) with number of cycles according to qPCR (usually Cq values were around 20) ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmids coding for segments 2 and 8 were digested using BbsI restriction enzyme (New England Biolab, NEB), the plasmid coding for segment 10 was linearized with BsaI (NEB) ...
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bioRxiv - Neuroscience 2019Quote: ... excess gel was removed and embedded specimen were placed in digestion buffer (1X TAE, 0.5% Triton X-100, 0.8 M guanide HCL) with 8 units/ml Proteinase K (NEB) for 2 hours at 37 °C ...
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bioRxiv - Genomics 2019Quote: ... we amplified 20 ng of the library with HSS-pGL4_F and HSS-pGL4_R1 (Supplemental Table 8) using NEBNext High-Fidelity 2X PCR Master Mix (NEB) for 16 cycles ...
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bioRxiv - Microbiology 2020Quote: ... 8 cycles of PCR amplification were performed using NEBNext® Q5 Polymerase 2X Master Mix (New England Biolabs), according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer ...
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bioRxiv - Cell Biology 2020Quote: 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1x NEB 1 buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The final cDNA libraries were amplified (cycle number of 8) using NEBNext Multiplex Oligos for Illumina (NEB, E7335) and purified using SPRIselect size selection beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2022Quote: ... all samples were amplified by a 8-cycle PCR-program using Phusion High-Fidelity DNA Polymerase (NEB #M0530L) using primers 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3’and 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’ ...
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bioRxiv - Genomics 2023Quote: ... the oxidized DNA was purified with 1.8X Ampure XP beads and eluted in 8 μL elution buffer (NEB). The DNA was denatured by adding 2 μL 0.1 M freshly diluted NaOH and incubation at 50°C for 10min ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... MBP (mouse, 1:30,000, New England Biolabs), FLAG (mouse ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM MgCl2 and 4 units of RNase Inhibitor Murine (NEB, M0314). After 30 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μl of Murine RNase Inhibitor (40 U/μl, New England Biolabs) and 1 μl RtcB RNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μl of Murine RNase Inhibitor (40 U/μl, New England Biolabs) and 1 μl ProtoScript II RT (200 U/μl ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM EDTA and 40 U/ml RNAse inhibitor (New England Biolabs) at 4 °C for 5 min to remove non-specifically associated proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1 µL of murine RNase inhibitor (40,000 units/mL, New England Biolabs), 0.125 µL of T7 polymerase ...
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bioRxiv - Neuroscience 2022Quote: ... 40 U/mL of RNAse inhibitor (NEB M0314L, as we previously used31). Tissue was gently homogenized while on ice 15 times with pestle A followed by 15 times with pestle B ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 x Protease Inhibitor Cocktail (NEB) as described before (Sun et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The reaction was supplemented with 20 U of Murine RNase inhibitor (NEB) and incubated at 25°C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... 5 U/mL RNase Inhibitor (M0314S; New England Biolabs, Ipswich, MA, USA), EDTA-free protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.2 μl of Murine RNase Inhibitor (40 U/μl, New England Biolabs), 1 μl ProtoScript II RT (200 U/μl ...
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bioRxiv - Molecular Biology 2020Quote: ... with murine RNase inhibitor (New England Biolabs, catalog number M0314L, Ipswich, MA). Quantitative RT-PCR was conducted using the CFX96 Touch-Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... digest (200 U/ml) in presence of murine RNase inhibitor (NEB, #M0314L) and 4 U/ml Turbo DNase (Ambion ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.2 μl of RNase Inhibitor (Catalog No. M0314, New England BioLabs Inc.), and 8 μl of nuclease-free water ...
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bioRxiv - Biochemistry 2021Quote: ... 26ul 20x SSC solution (InvitrogenTM 15557044) and 15ul RNase inhibitor (NEB M0314L). The 2ml tube was put in 50 °C water bath overnight ...
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bioRxiv - Bioengineering 2022Quote: ... 0.5 of Murine RNase Inhibitor (purchased from New England Biolabs, cat# M0314S), 2 μL of FQ-labeled reporter (RNaseAlert substrate purchased from IDT ...
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bioRxiv - Genomics 2022Quote: ... supplemented with Murine RNAse inhibitor (1 U/μL, “RI”, New England Biolabs) and ribonucleoside vanadyl complex (RVC ...