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Citations for New England Biolabs :
801 - 850 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 1 μg ml−1 of template DNA was mixed with 1000 U ml−1 of T7 polymerase and 1.5 mM of each rNTP (New England Biolabs) in 40 mM Tris-HCl ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... UK) were then synthesized through in vitro transcription of 1 mg template with T7 polymerases (M0251, New England Biolabs). The sections were fixated in 4% PFA ...
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bioRxiv - Bioengineering 2021Quote: ... Resulting amplicons were denatured and reannealed in a thermocycler before nuclease reaction using T7 Endonuclease 1 (New England Biolabs) at 37C for 20min ...
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bioRxiv - Plant Biology 2020Quote: All recombinant CPK28 clones were transformed into lambda phosphatase-expressing BL21 (DE3) E.coli cells for the production of dephosphorylated proteins or into T7 Express cells (New England Biolabs) for production of hyperphosphorylated protein (53) ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNA was prepared in vitro by using the HiScribeTM T7 high–yield RNA synthesis kit (New England Biolabs) and purified using an RNeasy MinElute Cleanup kit (QIAGEN) ...
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bioRxiv - Developmental Biology 2022Quote: ... In vitro transcription of gRNA was performed using the T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S) and generated gRNA was purified ...
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bioRxiv - Neuroscience 2022Quote: ... Cas9 mRNA and sgRNAs were synthetized and purified by HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, NEB) and Purification of Synthesized RNA (E2040 ...
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bioRxiv - Microbiology 2022Quote: ... and crRNAs that were synthesized commercially (IDT) or with the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). dsDNA templates were amplified from plasmids and purified using DNA Clean and Concentrator Kit (Zymo) ...
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bioRxiv - Immunology 2020Quote: ... Amplicon DNA was denatured and reannealed in a thermocycler prior to cleaving by T7 Endonuclease 1 (New England Biolabs) at 37°C in a 30min reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then transcribed from the NsiI-linearised plasmids with the HiScribe™ T7 ARCA mRNA Kit (NEB E2065S) or HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (NEB E2080S ...
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bioRxiv - Molecular Biology 2019Quote: All plasmids were linearized with NotI and transcribed in vitro using the HiScribe(tm) T7 ARCA mRNA kit (NEB). mRNA was purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2021Quote: RNA triggers were transcribed from linear DNA template using T7 polymerase according to the manufacturer’s protocol (New England Biolabs). Following RNA synthesis ...
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bioRxiv - Cell Biology 2021Quote: sgRNAs were generated by in vitro transcription using the Hiscribe T7 high yield RNA synthesis kit (New England Biolabs). Purified sgRNA (0.5 μg ...
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bioRxiv - Molecular Biology 2019Quote: All mRNAs were synthesized by T7 RNA polymerase in vitro transcription reaction (IVT) (New England Biolabs cat. no. M0251L) and purified using standard techniques ...
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bioRxiv - Biophysics 2021Quote: ... The in vitro mRNA synthesis was done using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA) or the mMESSAGE T7 ULTRA Transcription Kit (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: PCR products were subject to in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB), which relies on the T7 RNA polymerase to initiate transcription from a T7 promoter sequence (present in our fragments) ...
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bioRxiv - Microbiology 2022Quote: ... which was synthesized using the HiScribe T7 High Yield RNA Synthesis Kit according to manufacturer’s guidelines (New England Biolabs). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... Digested sgRNA and vector were incubated at room temperature for 1.5 hours in the presence of T7 ligase (New England Biolabs). Plasmids were transformed into transformation competent DH5alpha E ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro transcription was performed using HiScribe™ T7 or SP6 High Yield RNA Synthesis Kit (New England Biolabs). RNA was subsequently purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Biochemistry 2022Quote: ... 1 μg ml−1 of template DNA was mixed with 1000 U ml−1 of T7 polymerase and 1.5 mM of each rNTP (New England Biolabs) in 40 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... a product flanking the sgRNA target sites was PCR-amplified and subjected to a T7 Endonuclease I assay (NEB) to detect indels ...
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bioRxiv - Neuroscience 2022Quote: ... a DNA template was generated by PCR and then used for RNA synthesis (HiScribe T7 RNA polymerase, NEB #E2040S). DNA template was removed from the reaction with Turbo DNAse (Invitrogen #AM2238) ...
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bioRxiv - Synthetic Biology 2022Quote: ... T7 RNA polymerase (M0251) and murine RNase inhibitor (M0314) were obtained from New England Biolabs (NEB, Ipswich, MA, USA). gBlock Gene Fragments were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Molecular Biology 2022Quote: ... and in vitro transcription was performed using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA, USA) to generate IVT mRNA according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Vector DNA was dephosphorylated using quick cow intestinal phosphatase (QCIP) and ligation reactions conducted using T7 DNA ligase (NEB). Purification of DNA products was done by PCR cleanup (E.Z.N.A Cycle Pure Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNAs were generated by in vitro transcription using the HiScribe T7 high-yield RNA synthesis kit (New England Biolabs). Purified sgRNA (0.5 µg ...
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bioRxiv - Cancer Biology 2023Quote: ... Strings served as templates for the IVT and polyadenylation of RNA molecules as per manufacturer’s instructions (HIScribe T7 ARCA mRNA kit, NEB). Polyadenylation and integrity were assessed by gel electrophoresis in denaturing conditions and RNA was quantified with a Qbit fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 7.5 µL DNA was incubated (14 h, 37°C) with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix ...
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bioRxiv - Microbiology 2023Quote: ... nLuc plasmids were linearized using SpeI and subjected to in-vitro transcription using HiScribe T7 kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR amplified product was in-vitro transcribed using the HiScribe T7 High Yield RNA synthesis kit (NEB #E2040S) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: The editing activity of each Cas9 vector was assayed using T7 endonucleaseI (T7EI) (New England Biolabs, Ipswich, MA, USA) as described previously ...
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bioRxiv - Genetics 2023Quote: ... The sgRNA was synthesized using the NEB HiScribe T7 Fast and Efficient RNA Synthesis Kit (NEB E2050S, MA, USA). After mixing 5μM~10μM sgRNAs of bmp6a and bmp6b and 5μM Cas9 protein (M0646 ...
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bioRxiv - Developmental Biology 2023Quote: ... The sgRNA target region was amplified from transfected cells and analyzed by T7 endo1 nuclease assay (New England Biolabs). sgRNA ...
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bioRxiv - Microbiology 2023Quote: ... hysA (SAA6008_RS12255) and vigR 3’ UTR from JKD6008 were in vitro transcribed (IVT) using HiScribe T7 RNA polymerase (NEB). IVT products were RQ1 DNase treated (Promega ...
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bioRxiv - Biophysics 2024Quote: ... The cDNAs were transcribed in vitro to cRNAs using the HiScribe® T7 Arca mRNA Kit (New England BioLabs). Single mutations in Cx26 (W3A ...
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bioRxiv - Developmental Biology 2024Quote: ... Two guide RNAs targeting chordin and Three guide RNAs targeting tyrosinase were transcribed using T7 RNA polymerase (NEB #M0251s) from DNA templates made from the following primers for chordin ...
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bioRxiv - Developmental Biology 2023Quote: ... The gRNA was made by in vitro transcription using a Hiscribe T7 high yield kit (New England Biolabs #E2040s) using the following primers (Integrated DNA Technologies) ...
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bioRxiv - Synthetic Biology 2024Quote: ... In vitro transcription (IVT) eactions were performed with HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs). Template DNA was degraded with TURBO DNase (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... T7 transcription was performed for 16 h and then RNA was purified using the Monarch RNA Cleanup Kit (NEB). In vitro cleavage was performed with purified recombinant ...
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bioRxiv - Microbiology 2023Quote: ... Enzymes BsaI-HF®v2 (#R3733S) and BsmBI-v2 (#R0739S) (10.000 U/mL) and T7 ligase (#M0318S) were obtained from New England Biolabs (NEB). PCR amplicons were first cloned into the pYTK001 entry vector before assembly into a composite construct using preassembled vector backbones with GFP dropouts ...
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bioRxiv - Cancer Biology 2023Quote: ... a Poly(A) tailing reaction was performed for 1 hour according to the HiScribe T7 ARCA manual (NEB, E2060S). Subsequently ...
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bioRxiv - Synthetic Biology 2023Quote: ... In vitro transcription of sgRNAs was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... upon which half of the samples were treated with 1U (0.1 µl) of T7 Endonuclease I (New England Biolabs), which cleaves branched DNA structures ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the GFP sequence were generated by using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, E2060). 1 µg mRNA was first denatured at 65°C for 3 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... In vitro transcription was performed using the HiScribe T7 ARCA mRNA (with tailing) kit (New England Biolab, http://www.NEB.com) and Xenopus oocytes were injected with 30 ng of RiSKC3 cRNA using a pneumatic injector ...
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bioRxiv - Bioengineering 2024Quote: ... Nluc mRNA was synthesized using the HiScribe T7 high yield RNA synthesis kit (New England Biolabs Inc., MA, USA) with CleanCap Reagent AG (TriLink Biotechnologies ...
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bioRxiv - Cell Biology 2024Quote: MHC-ftz-Δi mRNA was synthesized in vitro using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) with MHC-ftz-Δi in pcDNA3.0 (30 ...
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bioRxiv - Developmental Biology 2024Quote: ... and mRNA was transcribed in vitro from the templates using HiScribe T7 ARCA mRNA Kit with Tailing (NEB, E2060) or mMESSAGE mMACHINE SP6 Transcription Kit (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: Isotope labeled firefly luciferase (Fluc) mRNA was synthesized by HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB, Ipswich) using stable heavy isotopes of ATP and CTP (NLM-3987-CA and CNLM-4267-CA-20 ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...