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Citations for New England Biolabs :
751 - 800 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently in-vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit from NEB (#E2050S).
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bioRxiv - Evolutionary Biology 2019Quote: ... Ligation was performed on a 2 mL reaction scale with 1X T7 DNA Ligase buffer (New England Biolabs), 4 µM dsDNA adaptor with two different 4 base sticky-ends ...
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bioRxiv - Genetics 2020Quote: ... pyogenes chimeric single guide RNA through in vitro transcription using a HiScribe T7 Quick kit (New England Biolabs). The gRNA was then precipitated in a 1/10 volume of 3M Sodium Acetate and two volumes of 100% ethanol by chilling the reaction at −20 °C for 15 min ...
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bioRxiv - Immunology 2020Quote: ... followed by synthesis of the covalently closed circular DNA (cccDNA) with T7 DNA polymerase and T4 ligase (NEB). Finally ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μg of DNA library was used as a template for mRNA synthesis by HiScribe T7 kit (NEB). The product was purified by NH4Ac precipitation and dissolved in RNAse-free water to a final concentration of 3 μg/ul.
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bioRxiv - Cell Biology 2020Quote: ... and subsequently in-vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit from NEB (#E2050S). The IRE-1 RNase-specific inhibitor 4μ8c was used at 5 μM.
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bioRxiv - Bioengineering 2021Quote: ... sgRNA was transcribed in vitro using the HiScribe Quick T7 High Yield RNA Synthesis Kit (New England Biolabs). The transcribed sgRNA was treated with DNaseI (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Capped SAM was synthesized in vitro using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs) and purified using a RNeasy Maxi Kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... in vitro transcription of BNT162b2 mRNA was performed with a HiScribe T7 ARCA mRNA Kit with tailing (NEB) with 2.5 mM N1-Methylpseudouridine-5’-triphosphate nucleoside analog (TriLink BioTechnologies ...
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bioRxiv - Microbiology 2022Quote: ... dsRNA was generated using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, New England Biolabs, Ipswich, Massachusetts), purified using the Monarch RNA Cleanup Kit (T2050S/L ...
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bioRxiv - Genetics 2022Quote: ... The RNA was transcribed using 1ug of template with the HiScribe T7 Quick High Yield kit (NEB, E2050) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Genomics 2022Quote: ... The purified product was then in vitro transcribed using “HiScribe T7 High yield RNA Synthesis Kit (NEB, E2040S) and purified using Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Molecular Biology 2023Quote: The dsRNAs were transcribed from these templates using HiScribe T7 high yield RNA synthesis kit (New England Biolabs). The dsRNAs were then treated with DNase and purified using RNA Clean & Concentrator-25 (Zymo Research).
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bioRxiv - Neuroscience 2023Quote: ... and then transcribed using pooled in vitro transcription (HiScribe T7 High Yield RNA Synthesis kit, New England BioLabs) and cleaned up with the NEB Monarch RNA Cleanup kit ...
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bioRxiv - Biochemistry 2022Quote: Selenomethionine-derivatized FL-TgGAC was expressed in the methionine auxotroph T7 Express Crystal Escherichia coli strain (NEB #C3022) cultured in SelenoMethionine Medium Base plus Nutrient Mix (Molecular Dimensions MD12-501) ...
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bioRxiv - Cancer Biology 2023Quote: ... The single cell colonies were screened by T7 Endonuclease I (T7E1, New England Biolabs, Ipswich, USA, Cat#M0302L) assay ...
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bioRxiv - Microbiology 2023Quote: Purified vigR 3’ UTR amplified from JKD6008 was in vitro transcribed (IVT) using HiScribe T7 RNA polymerase (NEB). RNA products were DNase I treated (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... 10µL ligation mix was prepared (20ng digested pX552 vector, 0.8µL 1:50 diluted oligo mix, 5µL 2X T7 Buffer (BioLabs #B0318S), 0.5µL T7 DNA Ligase (BioLabs #M0318S ...
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bioRxiv - Microbiology 2023Quote: ... These constructs were placed under the control of the T7 promoter and inserted into EcoRI (New England Biolabs) and NotI (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... and then transcribed using pooled in vitro transcription (HiScribe T7 High Yield RNA Synthesis kit, New England BioLabs) and cleaned up with the NEB Monarch RNA Cleanup kit ...
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bioRxiv - Cell Biology 2023Quote: Ifnb1 mRNA 3’ UTR (NM_002176.4) was synthesized using the HiScribe T7 High Yield RNA synthesis kit (NEB, E2040S) through in vitro transcription ...
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bioRxiv - Molecular Biology 2023Quote: Pre-crRNA arrays (Supplementary Table 9) were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB). T7 transcription was performed for 16 h and then RNA was purified using the Monarch RNA Cleanup Kit (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... the products were used to generate RNA transcripts using the HiScribe T7 In Vitro Transcription Kit (NEB, E2040S) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Template pDNA was linearized and ARCA-capped IVT-mRNA was produced using the T7 HiScribe kit from NEB, pseudo-uridine was incorporated into the reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... gel purified and transcribed with DIG RNA labeling mix with either T7 RNA polymerase (NEB, M0251S; antisense probe), or SP6 RNA polymerase (NEB ...
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bioRxiv - Immunology 2024Quote: ... IVT DI was in vitro transcribed using a HiScribe T7 High Yield RNA synthesis kit (New England Biolabs) followed by DNAse I clean-up with a TURBO DNA-free kit (Thermo-Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... and in vitro transcription and polyadenylation was performed using HiScribe T7 ARCA mRNA Kit (with tailing) from NEB, following recommended procedure ...
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bioRxiv - Evolutionary Biology 2020Quote: Purified DNA library (250 ng) was cut with the Hind III and Bam HI restriction enzymes (NEB) for 1h at 37 °C in a reaction mixture containing 1X of the NEB cut smart buffer ...
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bioRxiv - Cell Biology 2022Quote: ... pET17b-Kif5b(1-560)-GFP-His was transformed into BL-21[DE3]RIPL cells (New England Biolabs) until an optical density at 600 nm of 0.6-0.8 and expression was induced with 0.5 mM isopropyl-β-d-thiogalactoside (IPTG ...
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bioRxiv - Genomics 2020Quote: ... We put the mutagenized genes in a pIVEX vector via Gibson assembly (NEB Hi-Fi DNA assembly) using 125ng of gene DNA ...
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bioRxiv - Biochemistry 2021Quote: ... and C-terminal additions) were assembled with the pcDNA5 backbone using Hi-Fi DNA assembly (NEB; E2621S).
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bioRxiv - Plant Biology 2022Quote: ... His-TAD1 fusion proteins were purified according to the manufacturer’s protocol (New England Biolabs, Ipswich, MA, USA). The bait protein GST-WL1 was incubated with GST beads (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... the product was cloned into the pET1-5b N-terminal 6×His expression plasmid (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... The His-GST tags were cleaved by incubating the pooled fractions with TEV protease (New England Biolabs) at 4°C for 16h ...
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bioRxiv - Genetics 2019Quote: ... 2012) by removing cbr-Pmyo-2∷gfp∷his-72 UTR by cutting using KpnI and ApaI (NEB). The digested pZZ0031 backbone carrying cbr-unc-119(+ ...
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bioRxiv - Bioengineering 2022Quote: ... The t-NLuc-His tag was double-digested with NdeI and XhoI restriction enzymes (New England BioLabs), and the RNA sensor region were digested with SgrAI and SacII restriction enzymes ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (Table S1) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were constructed in the pUC19 backbone using NEBuilder Hi-Fi DNA Assembly Mastermix (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA (28) using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (sTable 5) ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Developmental Biology 2020Quote: ... Antisense digoxigenin-labelled RNA probes were then obtained by in vitro transcription using T7 RNA polymerase (New England Biolabs) and digRNA labelling mix (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... and directly used for in vitro transcription of single-guide RNAs (sgRNAs) with a T7 Polymerase mix (M0255A NEB). All sgRNA reactions were treated with RNAse free-DNAse ...
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bioRxiv - Neuroscience 2021Quote: RNAs were in vitro transcribed from PspOMI-linearized pcDNA3.1(+) reporter plasmids using HiScribe T7 High Yield RNA Synthesis Kit (NEB) with 3’-O-Me-m7GpppG anti-reverse cap analog (ARCA ...
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bioRxiv - Molecular Biology 2021Quote: sgRNA products synthesized by T7 RNAP WT and S43Y mutant were purified with RNA purification kits (New England Biolabs) before analyzed by high performance liquid chromatography (HPLC ...
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bioRxiv - Molecular Biology 2020Quote: ... SSS reaction products (16 μl) were combined with 24 μl T7 High Yield RNA Synthesis Kit (NEB, Cat #E2040) reagent mix containing 4 μl T7 Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... in vitro transcription was performed to produce sgRNAs using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). Next ...
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bioRxiv - Biochemistry 2020Quote: ... encompassing a T7 promoter followed by hammerhead ribozyme and the specific 5’ tRNA isoacceptor or scrambled RNA sequences were PCR-amplified and afterwards in vitro-transcribed using 1.875 U/µL T7 Polymerase (NEB) in 1x T7-Buffer with 20 mM DTT ...
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bioRxiv - Genomics 2019Quote: ... The template was then used for in-vitro transcription using the HiScribe T7 High Yield RNA synthesis kit (NEB). The in-vitro transcription reaction was carried out at 37°C for 16 hrs ...
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bioRxiv - Cancer Biology 2020Quote: ... SALL4-targeting sgRNA candidates were transcribed with HiScribe™ Quick T7 High Yield RNA Synthesis Kit (New England Biolabs) following manufacturer’s instructions ...