Labshake search
Citations for New England Biolabs :
8251 - 8300 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Genomic DNA was purified from 100μL of CSF samples using the Monarch® Genomic DNA Purification Kit (New England Biolabs, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Substrates were transcribed in vitro from purified PCR products using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Pooled genomic DNA samples were used to sequence libraries constructed using the NEBNext Ultra II Library Prep Kit (NEB, Ipswich, USA). Paired-end metagenomics sequencing was performed on an Illumina NextSeq 550 sequencer using NextSeq High Output Kit v2 sequencing reagent kit ...
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bioRxiv - Microbiology 2023Quote: ... 300 bp-insert RNA-seq Illumina libraries were constructed using ∼1.0 μg of enriched mRNA that was fragmented then used for synthesis of strand-specific cDNA using the NEBnext Ultra Directional RNA Library Prep Kit (NEB-E7420L). The cDNA was purified between enzymatic reactions and the size selection of the library performed with AMPure SpriSelect Beads (Beckman Coulter Genomics) ...
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bioRxiv - Immunology 2023Quote: ... DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Cell Biology 2023Quote: Libraries were prepared on an automated liquid handler (Biomek i7) using the NEBNext Ultra II DNA library preparation kit (NEB, E7645), according to the manufacturer’s instructions and without size selection ...
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bioRxiv - Genomics 2023Quote: ... Paired-end libraries were constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs, E7645S) using a starting material of 50 ng ...
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bioRxiv - Molecular Biology 2023Quote: ... with primers listed in Table S8, then inserted into PHR-mCh- CryWT plasmid (Adgene, 101221) by using NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S). The generated constructs were fully sequenced to confirm the absence of any mutations or stop codons ...
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bioRxiv - Microbiology 2023Quote: ... dual unique indexed libraries for sequencing on all Illumina platforms were made using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Manufacturer protocol was modified by diluting adapter 1:30 and using 3 μl of this dilution ...
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bioRxiv - Microbiology 2023Quote: Libraries for RNAseq were prepared using the NEB Next Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA). Individual libraries were uniquely barcoded with NEBNext Multiplex Oligos for Illumina sequencing platform (New England Biolabs ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Libraries were prepared for both the INPUT and IP RNA using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina) (NEB, #E6420S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Total of 3 μg RNA was prepared for sequencing libraries using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) according to manufacturer’s instructions and sequences attributed to each sample by adding index codes ...
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bioRxiv - Microbiology 2023Quote: ... The preparation of the sRNA-enriched library was done with NEBNext Ultra II RNA kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s recommendation which included cDNA synthesis ...
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bioRxiv - Neuroscience 2023Quote: ... Ribo-depleted RNA sequencing (RNA-seq) libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7765S). RNA samples were fragmented ...
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bioRxiv - Cancer Biology 2023Quote: ... NEBNext Low Input RNA libraries were prepared manually following the manufacturer’s protocol (NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® Instruction Manual Version 5.0_5/20, NEB #E6420L). Samples were normalized to 1ng total RNA material per library in 8μl of nuclease-free water ...
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bioRxiv - Developmental Biology 2023Quote: ... building parallel total-RNA and poly(A)+ Illumina strand-specific libraries using the Ultra II RNA-seq library kit (NEB #E7765) (see below) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were made with 6 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The manufacturer’s protocol was adjusted to account for shorter DNA fragments as described previously 45.
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bioRxiv - Molecular Biology 2023Quote: ... Up to 0.5ng of DNA from KTRC donor samples (3399, 3409, 3447) was used to prepare sequencing libraries using NEBNext Ultra II Library Kit (NEB, E7645), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The Lysobacter gasdermin system was previously cloned10 and the Pedobacter rhizosphaerae system was amplified from genomic DNA ordered from the DSMZ (DSM 18610, IMG gene accessions in Table S2) and cloned using the NEBuilder HiFi DNA Assembly cloning kit (NEB E5520S). As a negative control ...
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bioRxiv - Bioengineering 2023Quote: ... Start codon of MAAP-WT2 (CTG) was substituted with ATG using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) in accordance with manufacturer’s protocol.
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bioRxiv - Biophysics 2023Quote: ... The R203M N175-245 plasmid was prepared from the WT N175-245 plasmid with site directed mutagenesis (NEB site mutagenesis kit). The plasmids were transformed into BL21(DE3 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was purified by PolyA selection and libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (NEB, E7770). Samples were sequenced on a NextSeq 2000 (Illumina).
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bioRxiv - Neuroscience 2023Quote: ... The gRNAs were cloned into a dual gRNA pCFD4 vector having a BbsI restriction site using Gibson Assembly Kit (New England Biolabs (UK) Ltd ...
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bioRxiv - Cell Biology 2023Quote: ... the efficiency of each sgRNA (sg1 to sg5) was verified by extracting genomic DNA (Monarch Genomic DNA Purification Kit, NEB T3010S) and PCR-amplifying the region of the gene targeted by each sgRNA ...
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bioRxiv - Microbiology 2023Quote: ... Pooled total DNA samples were used to construct libraries using the NEBNext Ultra II Library Prep Kit (NEB, Ipswich, MA, USA). Paired-end shotgun metagenome sequencing was performed on a NextSeq 550 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... 100ng of polyA selected mRNA samples were treated using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S) according to the manual ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of linearized plasmid was used as template in a 10 µL reaction using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) with an 8:1 ratio of cap analog to GTP ...
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bioRxiv - Genetics 2023Quote: RNA was isolated from frozen hippocampal samples stored at -80°C using the Monarch® RNA Total Isolation kit (NEB T2010S). Hippocampus from the left hemisphere was used where possible ...
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bioRxiv - Plant Biology 2023Quote: ... The purified total RNA was used to synthesize cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, catalog number E3010).
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bioRxiv - Neuroscience 2023Quote: ... the reporter and a barcode unique to each enhancer sequence using the NEBuilder® HiFi DNA Assembly Cloning Kit (NEB-E5520S), following standard protocol ...
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bioRxiv - Genomics 2023Quote: ... samples were split into 5 μg aliquots for sequencing library preparation using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, E7645L) and NEBNext Multiplex Oligos (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... The linear backbone vector (2,183 base pairs in size) was gel extracted using a gel extraction kit (NEB, Cat. No. T1020S) and assembled with the pegRNA oligos (listed above ...
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bioRxiv - Genomics 2023Quote: ... In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs, no. E2070S), purified using the Monarch RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... The digested fragments were gel-purified and then cleaned up using a GenepHlow™ Gel/PCR Kit before ligation into the appropriate vector using T4 ligase (New England Biolabs). The ligated plasmids were transformed into DH5α ...
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bioRxiv - Cell Biology 2023Quote: ... G3BP1-GFP (V11A, F112A, F33A, F33W, F15A, F15W, F124A, F124W) were created by the Q5 Site-Directed Mutagenesis kit (NEB; E0554S).
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bioRxiv - Developmental Biology 2023Quote: ... Libraries for RNA-Seq were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760) and sequenced on the NextSeq500 (Illumina ...
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bioRxiv - Bioengineering 2023Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Cat. No. / ID: E7770) following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... full length CDS of TaSnRK1α gene was amplified using the wheat cDNA and cloned into the pCAMBIA1302 vector under the constitutive promoter 35S using the Gibson assembly cloning kit (Gibson Assembly® Master Mix, NEB) with the help of restriction enzyme NcoI ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-Seq was performed using libraries prepared with NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on the Illumina NextSeq 500 platform to obtain 36bp paired-end reads ...
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bioRxiv - Neuroscience 2023Quote: ... ChIP-Seq was performed using libraries prepared with the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... we introduced a silent mutation into the PAM motif of the sgRNA located within the 3’ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054). The donor plasmid was confirmed with DNA sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Corresponding site-directed mutagenesis was conducted on the intermediate TOPO constructs with specific mutation primers by using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). The mutated E2- or E1-containing fragments were digested from the TOPO constructs through intrinsic viral restriction sites and inserted back into CHIKV through T4 ligation.
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bioRxiv - Molecular Biology 2023Quote: ... from 1 µg of total RNA and library preparation was conducted with the NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB) with Dual Index Primers (NEBNext® Multiplex Oligos for Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... RNA libraries were prepared using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs) and sequenced using the Illumina NOVAseqSP sequencer.
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bioRxiv - Microbiology 2023Quote: ... Parasites were pelleted by centrifugation at 13,000 xg for five minutes and the parasite DNA was isolated using the Monarch® Genomic DNA Purification Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: RNA was used as template to detect and quantify viral genomes by duplex reverse transcriptase (RT) quantitative polymerase chain reaction (RT-qPCR) using a Luna Universal Probe one-step RT-qPCR kit (New England Biolabs, E3006E). SARS-CoV-2-specific RNAs were detected by targeting the ORF1ab gene using the following set of primers and probes ...
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bioRxiv - Microbiology 2023Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (96) (New England BioLabs, Germany). To assess quality and size of the libraries ...
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bioRxiv - Physiology 2023Quote: ... which were then used to replace the corresponding regions in the wildtype plasmids with NEBuilder® HiFi DNA Assembly Cloning Kit (New England BioLabs). All plasmids were fully sequenced ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNAs were subcloned downstream of a tetracycline response element (TRE) using the Gibson Assembly Cloning kit (New England Biolabs, # E5510S). Mutations were introduced by in vitro mutagenesis using Q5 Site-directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... RNA extraction was done with TriZol from the 12 fractions collected and then samples were cleaned up using the Monarch® RNA Cleanup Kit (50 μg) from New England Biolabs (NEB). Optical densities (OD260 ...