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Citations for New England Biolabs :
8051 - 8100 of 8151 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... PCR product were circularized by intra-molecular self-ligation in 1 ml of ligation buffer using T4 DNA ligase (New England Biolabs, Ipswich, MA). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... Additional reconstitutions with linker histone were performed by mixing reconstituted 183×12 core arrays with 1 mg/ml recombinant linker histone variant H10 (New England Biolabs, cat.# M2501S) at a molar ratio of 0.7 molecule histone H1 per nucleosome in solution containing 500 mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... PCR products (∼1.1 kb-long flanking unique sequences on either side of each rDNA locus) were labelled using Klenow Fragment exo- (NEB CAT. M0212L) with either Biotin-16-dUTP (Roche CAT ...
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bioRxiv - Cell Biology 2022Quote: ... INS-1 832/3 SNAP- GLP- 1R or SNAP-GIPR cells were labelled in suspension with 40 nM SNAP- Lumi4- Tb and 1 mM SNAP- Surface 649 (New England Biolabs, Hitchin, UK) for 1 hr at room temperature in complete medium ...
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bioRxiv - Genomics 2022Quote: ... samples were gently removed from the chamber and digested overnight at 37°C in 8 U mL-1 proteinase K (NEB, cat# P8107) in digestion buffer (1× TAE buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Molecular Biology 2023Quote: ... The digested T/F LTR U3R region was cloned into the linearized pGL3 Basic vector/plasmid and ligated using 1 U of T4 DNA ligase (New England Biolabs, MA, USA) as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: RE digestion reactions are prepared by adding 1/10th of the final reaction volume of 10x reaction buffer supplied by the RE manufacturer (typically NEB CutSmart buffer) and 0.1% Tween-20 to the DNA solution (diluted to the desired concentration in nanopure DI water) ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was purified from 1 μg of total RNA using an NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... the upstream and downstream probes of DLP pairs was ligated by applying a ligation mix containing 1× SplintR buffer (Cat No. B0375S; NEB, Beijing, China), 2.5 U/μL SplintR Ligase (Cat No ...
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bioRxiv - Cell Biology 2022Quote: ... PRR14 1-212 PCR products were sub-cloned into the expression vector pCMV6-AN-mGFP using T7 ligase (New England Biolabs, cat# M0318S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The kinesin and GBP constructs were exchanged into 1x PBS with 1 mM DTT and labeled with DNA and SNAP-Surface Alexa Fluor 647 (NEB, Ipswich, MA) dye directly after elution ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was prepared with 1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalog #E6560L) as instructed by the manufacturer ...
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bioRxiv - Systems Biology 2024Quote: ... Eluted glycopeptides were lyophilized and then deglycosylated by incubation with 50 µL of 1 unit per microliter of PGNase F (New England Biolabs, Ipswich, MA) in 50 mM Tris-HCl (pH=7.5 ...
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bioRxiv - Immunology 2024Quote: ... The region after the TRR to the middle of exon 1 was amplified from 50 ng of gDNA using Phusion® High-Fidelity polymerase (New England Biolabs, M0530) according to the manufacturer’s instructions with the following primers ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genomics 2024Quote: ... RNA from each sample was enriched from 1 µg total RNA by the NEBNext® Poly(A) mRNA Magnetic Isolation Module (Catalog #: E7490, New England Biolabs Inc.). RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog # ...
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bioRxiv - Microbiology 2024Quote: ... GST-IpaH4-3XFlag was cloned into pGEX6P-1 with a 3XFlag peptide followed by the coding sequence for ubiquitin using Gibson Cloning (NEB; Table S4). Sequence-verified constructs were transformed into E ...
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bioRxiv - Microbiology 2024Quote: ... was reverse transcribed from ∼1 μg high quality RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEW ENGLAND BioLabs, Germany). Newly synthesized cDNA was purified using a the NucleoSpin® Gel and PCR Clean-up kit (Germany ...
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bioRxiv - Microbiology 2023Quote: ... and lysed with 2× concentrated lysis buffer (0.25% Triton X-100, 50 mM KCL, 100 mM Tris-HCl pH 7.4, 40% glycerol, 1/50 volume of RNase inhibitor; NEB, Cat. No. M0314S) at room temperature for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... in vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer with 5 μg RNA substrate (1.8 knt. Same substrate of MTase-Glo experiment) and 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA), 1 μM methyltransferase or no enzyme as a negative control ...
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bioRxiv - Synthetic Biology 2023Quote: ... The pUdO#a was obtained by assembling four fragments (Table 1 and Figure S1) using the Gibson Assembly® Master Mix (New England Biolabs, E2611) according to the supplier’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... 1 µg of the duplex was in vitro transcribed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB # E2050S) following the standard IVT protocol and purified using Monarch® RNA Cleanup columns (NEB # T2030).
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bioRxiv - Evolutionary Biology 2023Quote: ... we purified the products of the ace-1 PCR (see above) using the BS664-250 Preps EZ-10 Spin Column PCR Purification kit (New England BioLabs, Evry France). The purified PCR products were then cloned (TOPO TA Cloning Kit pCR 2.1-TOPO Vector and TOP10F’ invitrogen bacteria) ...
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bioRxiv - Genetics 2024Quote: ... HMW DNA was also isolated from six PBMC samples (± 1 x 106 cells) using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, #T3050L) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Linker ligation at 3’ ends was set up per sample after washes following alkaline phosphatase treatment by preparing a T4 RNA ligase 1 (NEB, cat. #M0204S) reaction in 40μL following the manufacturers’ instructions with 100pmol of radiolabeled L32 RNA linker ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were digested in a 20 μl reaction with NEB Cutsmart buffer for 30 minutes - 1 hour with SmaI (NEB, Cat# R0141) to assess rDNA promoter methylation or with HpaII (NEB ...
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bioRxiv - Cancer Biology 2023Quote: 1 µg total RNA was used for NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Poly-A selection and cDNA synthesis were performed according to NEB protocol ...
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bioRxiv - Biophysics 2023Quote: ... For each sample a separate chamber with closed nanosensors was prepared and filled with 1×CutSmart™ buffer (New England BioLabs, USA) containing 50 mM potassium ...
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bioRxiv - Plant Biology 2023Quote: Approximately 1 ug of RNA was used as input to the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, catalog #E7490S), followed by NEB Ultra II RNA library prep kit for Illumina (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... appropriate number of amplification cycles x (98C - 10sec, 63C – 30sec, 72C – 1 min) with NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) and barcoded Nextera primers (1.25 μM each ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, catalog #E6560S) with oligo-dT priming according to the manufacturer protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR reactions were run on a 1% agarose gel and positive bands were excised and purified using Monarch Gel extraction clean kit (NEB, Cat. #T1020L). A-tailing of the fragments was carried out by incubating the DNA with MyTaq polymerase (NEB ...
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bioRxiv - Genomics 2024Quote: The combined pool of purified DHS fragments (∼1 μg) was end repaired and ligated to NEBNext adaptor (included in NEB cat. # E7645S) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Transcription templates were prepared by PCR using primers complementary to the T7 promoter and terminator sequences (see Supplementary Table 1) or by digestion of the plasmid DNA with SphI (NEB, Cat# R3182S). mRNA fragments were prepared by in vitro transcription with T7 RNA polymerase using a ratio of 10:30 AU:GC NTPs ...
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bioRxiv - Molecular Biology 2024Quote: ... SEGS-1 region N was generated by cleaving the Kpn1 site using the Q5-site directed mutagenesis protocol (New England Biolabs, Ipswich, MA) from S1-1.5a (pNSB1829 ...
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bioRxiv - Molecular Biology 2024Quote: ... and joining SEGS-1F and SEGS-1G using the indicated primers in Supplementary Figure 1 and the Q5 high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). All the primers except for those for site-directed mutagenesis added NotI restriction sites at the 5’ and 3’ends of PCR products ...
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bioRxiv - Synthetic Biology 2024Quote: The reaction was brought up to 50 uL with the addition of CutSmart (final concentration 1X) and 1 uL DpnI (NEB, Catalog #R0176S) and incubated for 1 hour at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... We isolated poly(A)+ RNA from 1 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Detection of editing in the target genes was performed via amplifying the target regions (primers in Supplemental Table 1) with high fidelity DNA polymerase Q5 (New England Biolabs, Ipswich, MA, USA), followed by cloning of PCR products and sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were blocked with 5% BSA in 1X PBS for 30 minutes at room temperature followed by subsequent incubation with primary antibodies against MBP (NEB E8032L, 1:200) and RAD51 (Proteintech 14961-1-AP ...
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bioRxiv - Genetics 2022Quote: ... mRNA was purified and enriched from 1 µg of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, catalog no. E7490L). RNA fragmentation ...
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bioRxiv - Cell Biology 2022Quote: ... Halt™ Protease and Phosphatase Inhibitor Cocktail in 1X PBS) and then washed twice with either 11 dephosphorylation buffer (1 mM MnCl2, 1X PMP buffer, New England Biolabs, P0753, protease inhibitors) or CN dephosphorylation buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... GAL4-Creb5(1-128)T59/T61A and GAL4-Creb5(1-128)C18/C23S were generated by inducing point mutations in the GAL4-Creb5(1-128) vector using the Q5® Site-Directed Mutagenesis Kit (NEB, Cat#: E0554S). The control vector GAL4(1-147 ...
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bioRxiv - Genetics 2020Quote: ... The PCR products were run on a 1% agarose gel stained with ethidium bromide alongside a 100 bp ladder (New England Biolabs, Ipswitch, MA, USA).
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bioRxiv - Biochemistry 2020Quote: ... The PCR amplification was performed with 10 µl of the reverse transcriptase product in a 50 µl PCR mix containing 1 unit of Phusion High-Fidelity DNA polymerase (New England Biolabs, Évry-Courcouronnes, France), 1X HF Phusion buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... fraction #2 of all samples as well as a positive control of U1 snRNP were incubated with 1 μl of proteinase K (NEB, 800 units/ml) for 30 min at 37 °C and loaded onto a denaturing gel (8% acrylamide/bis-acrylamide ...
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bioRxiv - Pathology 2021Quote: ... The DNA fragment encoding the desired FLAG-TEV tag was generated via PCR using primer pair 2 (table 1) and subsequently cloned into the NdeI restriction site of pET24Δlac/Ep-CoV2 using NEBuilder® HiFi DNA Assembly (NEB, Ipswich, MA, USA).
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bioRxiv - Genomics 2020Quote: ... genomic DNA libraries were constructed for sequencing on Illumina platforms using the NEBNext® DNA Sample Prep Master Mix Set 1 (New England Biolabs, Ipswich, MA). First ...