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Citations for New England Biolabs :
7901 - 7950 of 8151 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... We added 1 mL of RAD reagent from the RAD002 kit and 0.2mL of Blunt TA ligase (New England Biolabs, MA, USA) and the reaction was incubated for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: Bacterial 16S rRNA genes were amplified from 50 ng DNA per sample diluted to 10 µL nuclease free water in 50 µL with 1 × LongAmp Taq master mix (New England Biolabs, Inc), 1 µL 16S rRNA gene barcoded primer (ONT-SQK-RAB-201 ...
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bioRxiv - Biochemistry 2019Quote: ... Immunoblotting was performed using HRP-conjugated anti-maltose binding protein (MBP) monoclonal antibody at 1:10000 dilution (New England Biolabs, #E8038) to verify equal expression levels of each construct.
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bioRxiv - Plant Biology 2019Quote: ... Expression of recombinant proteins was performed at 25°C for 4 h with 0,3 mM Isopropil-β-D-1-tiogalattopiranoside (IPTG) and affinity purification was obtained by using the amylose resin affinity matrix (New England Biolabs) in a buffer containing 60 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... A second adaptor was added to the cDNA by adding 50 pmol 5iLL-22 DNA adaptor (Table S6) and ligating with T4 RNA Ligase 1 (NEB, M0437M) at 23°C for 3 hrs ...
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bioRxiv - Molecular Biology 2020Quote: ... 46 separate 60 μl PCR reactions were performed with 1 μg gDNA in each reaction using Q5® High-Fidelity DNA Polymerase (New England Biolabs). For the zeocin treated sample the total amount of genomic DNA harvested was used ...
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bioRxiv - Microbiology 2020Quote: ... We prepared a library with a fragment size of 300 nucleotides and used the 10 μM NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335) lot 0091412 and the NEBNext multiplex Oligos for Illumina (Set 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μL of 10x SSS buffer and 1 μL of SSS Enzyme (NEBNext mRNA Second Strand Synthesis Module, New England Biolabs, USA) were added to the 17 μL of the purified sample ...
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bioRxiv - Microbiology 2020Quote: ... and run on an agarose gel in 0.5X Tris-borate-EDTA buffer at 70V alongside 1 Kb ladder (New England BioLabs, Ipswich, MA) or GeneRule Express DNA Ladder (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were purified from a 1% agarose gel using the Monarch DNA Gel Extraction kit (New England Biolabs, USA) and send for Sanger sequencing provided by Edinburgh Genomics ...
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bioRxiv - Genetics 2020Quote: ... was annealed with 1ul of the reverse sgRNA (100 uM) using 1 ul 10X T4 ligation buffer (New England Biolabs, NEB), 1 ul T4 PNK (NEB) ...
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bioRxiv - Genomics 2020Quote: ... 1 μM of NEBNext Unique Dual Index Primers and 25 μl NEBNext Q5U Master Mix (M0597, New England Biolabs, Ipswich, MA) were added to the DNA and amplified as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 pg of lhx1a:DsRed/pI-SceI plasmid DNA was injected into 1-cell stage embryos along with the I-SceI restriction enzyme (NEB, R0694S). These injected embryos were raised to adulthood and screened for DsRed expression in known lhx1a expression domains and isolated a stable Tg(lhx1a:DsRed ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Biochemistry 2021Quote: Nucleosome remodeling reactions (25 μL) were carried out in REA Buffer with 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP ...
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bioRxiv - Synthetic Biology 2021Quote: Expression units were assembled from cloned level 0 parts into level 1 destination vectors provided by the MoClo kit using 40fmol of each vector and 20 U of BsaI-HFv2 (NEB, R3733L) with remaining conditions as described above ...
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bioRxiv - Synthetic Biology 2021Quote: ... Vector and miRNA target inserts were ligated at 1:10 molar ratio using 400 U of T4 DNA Ligase (NEB, M0202) using provided Ligase buffer in a reaction volume of 20 µL over night at 16 °C ...
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bioRxiv - Cell Biology 2020Quote: Crushed lungs were homogenized using a mechanical homogenizer (Omni International, Waterbuy CT) or cultured cells were lysed in 1× lysis buffer (NEB, #9803S) supplemented with complete protease inhibitors (Roche ...
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bioRxiv - Genomics 2020Quote: ... The PCR was carried out with 1 μl of Lambda DNA (20 ng/μl; Catalog number: N3011S. Vendor: New England Biolabs Inc), 12.5 μl of KAPA 2X Master Mix ...
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bioRxiv - Genomics 2019Quote: ... We tested nuclear DNA removal by starting with 1 µg of total DNA using either 10 or 20 Units of Exonuclease V (New England Biolabs; UK) and an incubation of 30 minutes or 2 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA of 1×108 blood stage was extracted for conventional PCR amplification with a Q5® High-Fidelity DNA polymerase (New England Biolabs) from the interference inserts corresponding to the sense and anti-sense fragments of a portion of 429 bp AKT-like genomic sequence of T ...
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bioRxiv - Immunology 2021Quote: ... in YPD supplemented with 100 µg/ml ampicillin and 1% glucose (YPD-Amp-Glu) were infected with M13KO7 helper phage (New England Biolabs, USA) at 7×109 plaque forming unit (PFU ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5 kb and 500 bp DNA fragments were placed in two independent lines along with another line for 1 kb Ladder (NEB, N232). Once bands were clearly identifiable ...
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bioRxiv - Genetics 2019Quote: ... Poly(A)+ RNA was selected from 1 μg of total RNA using the NEBNext Ultra Kit (New England Biolabs, Ipswitch, MA). Libraries were prepared and sequenced by Genewiz (South Plainfield ...
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bioRxiv - Cell Biology 2019Quote: ... The cDNA coding region corresponding to RDGB(USR1-LNS2)Δ (amino acids 1-472) was subcloned into pUAST-attB by using the restriction enzymes NotI and XbaI (NEB). Similarly ...
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bioRxiv - Immunology 2022Quote: ... The library for sequencing was prepared using 1 µg of RNA and the NEBNext Ultra kit (New England BioLabs, Ipswich, MA), according to the protocol for low-input samples ...
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bioRxiv - Molecular Biology 2022Quote: ... Twelve restriction enzyme digests were prepared by combining 15 µL of each purified DNA sample with 1 µL DpnI (NEB R0176S) and 4 µL 10X CutSmart Buffer (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... encompassing the targeted loci were generated using 1 μl of the zebrafish lysate with Phusion® High-Fidelity Polymerase (New England Biolabs) and primers listed in Supplemental Table S3 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was diluted 1:25 in water and used as template for RT-qPCR using the Luna Universal qPCR master mix (NEB M3003S). Primers used are listed in Supplementary Table 3 ...
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bioRxiv - Genomics 2022Quote: The 2.2 kb DNA was ligated with 1 kb biotin- and dig-labeled DNA through SbfI- and XbaI-overhangs by T4 DNA ligase (NEB Biolabs). The ligated products were attached to the anti-dig treated surface of reaction chamber and streptavidin-coated magnetic beads ...
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bioRxiv - Synthetic Biology 2022Quote: ... which was also double checked visually in an agarose gel where the density of target bands was compared with the DNA ladder (1 Kb Plus, NEB N0469S).
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bioRxiv - Microbiology 2022Quote: ... Purified RNA was quantified by ultraviolet spectrophotometry and 1 μg used for reverse transcription by LunaScript® RT SuperMix Kit (New England Biolabs), as described by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... A polyadenylated fraction was purified from the total RNA (1 µg) using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). This fraction was used to construct cDNA library using a previously described method [91] ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR reactions were performed with a final primer concentration of 1 mM using Q5® High-Fidelity 2X Master Mix (NEB). Equal volumes of X and Y PCR reactions were then combined ...
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bioRxiv - Plant Biology 2022Quote: ... Double promoter constructs were constructed by reamplifying the promoter region of interest with unique overhangs and cloning into the Level −1 universal acceptor backbones using the BsaI-HFv2 restriction enzyme (#R3733L New England Biolabs, USA). These parts were then assembled into the same Promoter + 5’ UTR acceptor backbone using the BpiI restriction enzyme (#ER1012 Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... PolyA RNA was then enriched from 1 μg of DNase I-treated RNA using Oligo d(T)25 Magnetic Beads (NEB S1419S), followed by RNA-seq libraries construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB E7420) ...
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bioRxiv - Molecular Biology 2023Quote: The starting library strand (50 pmol) and regeneration hairpin (75 pmol) (Supp. Table 1) were combined in a 1X ligation buffer (New England Biolabs (NEB)) ...
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bioRxiv - Neuroscience 2023Quote: ... PCRs to confirm targeted and untargeted SMN2 loci were performed for each of the cell lines with primer pairs shown in table 1 using High-Fidelity 2X PCR Master Mix (NEB, M0541L) and Thermal Cycler C1000 Touch (Bio-RAD) ...
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bioRxiv - Genomics 2022Quote: ... 1 μg of gDNA was amplified in a 200 μl reaction using Q5® High-Fidelity 2X Master Mix (NEB #M0491). PCR master mix (100 μl Q5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Seven µL of DNA product was circularized in a reaction with 1 µL of 10X T4 DNA Ligase Reaction Buffer (NEB, #B0202A), 1 µL T4 Ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was incubated for 1 hour in the presence of an anti-SNAP-tag primary antibody (New England Biolabs #P9310S) diluted at 1:2000 in PBS + tween-20 0.1% ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were barcoded using Illumina i5 and i7 adaptors from NEBNext® Multiplex Oligos for Illumina (Dual Index Primers Set 1) kit (New England Biolabs) and sequenced using a 150-cycle NextSeq 500/550 High Output Kit v2.5 with 75 forward and 75 reverse reads ...
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bioRxiv - Immunology 2024Quote: ... To subclone this pool we digested our protospacer-empty Libr.1-null transfer vector with BsmBI-v2 (New England Biolabs, NEB) overnight at 55 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was quantified by Nanodrop and 1 µg of RNA per sample was treated for ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (NEB, E6350) and then immediately followed by Library prep using NEBNext Ultra II kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 13000 x g for 1 minute and the supernatant was once more purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs). We expect the “crush and soak” method to improve signal strength if low labeling densities are used during extension.
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bioRxiv - Cell Biology 2024Quote: ... PCR amplicons were loaded on to 1% (w/v) agarose gels stained with Midori Green Advance DNA stain (Geneflow #S6-0022) alongside 100 bp ladder (NEB #N3231S), before submitting for DNA Sanger sequencing (Source Genomics ...
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bioRxiv - Microbiology 2023Quote: ... The expression plasmid was assembled via Gibson Assembly by mixing pETDuet-1 vector digested with NdeI and PacI with the gBlock in addition to Gibson Assembly Mix (NEB #E2611L) and incubating at 50°C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were amplified for N-1 cycles (being N the optimum Cq determined by qPCR reaction) using NEBNext High-Fidelity Polymerase (New England Biolabs, M0541). Libraries were purified with Sera-Mag Select Beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared with 0.1-1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalogue #E6560L) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The genomic region around rbpms2aae30 was amplified for 35 cycles with an annealing temperature of 60°C and the wild-type allele was digested with HaeIII for 1 hour (New England Biolabs, R0108S)2 ...