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Citations for New England Biolabs :
751 - 800 of 5774 citations for Recombinant Human Endothelin Converting Enzyme 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... nascent RNA was isolated with streptavidin beads and barcoded adapters ligated at 3’ ends of the nascent RNA (T4 RNA ligase 1 enzyme, M0204L; NEB) overnight at 25° C ...
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bioRxiv - Cell Biology 2023Quote: ... The unedited methylated DNA template present in the remaining sample was digested for 1 h at 37°C with DpnI restriction enzyme (New England Biolabs) in CutSmart® Buffer (New England Biolabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... The genomic region around the miossa20 allele was amplified for 35 cycles using 57°C for annealing and identified by restriction enzyme digestion of the wild-type allele with BsaWI for 1 hour (New England BioLabs). The genomic region surrounding the spo11uc73allele was amplified for 35 cycles with an annealing temperature of 57°C and products were resolved without digestion48 ...
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bioRxiv - Immunology 2021Quote: ... and the fragment was assembled using Hi-Fi DNA Assembly Mix (NEB) into the donor vector pADN171XS7 ...
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bioRxiv - Microbiology 2021Quote: ... His-MBP-MirA were first isolated using amylose bead affinity chromatography (NEB) in conditions specified by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... The homology plasmids were assembled using HI-Fi DNA Assembly Mastermix (NEB) with four PCR-generated DNA fragments (see Table S2 for primers) ...
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bioRxiv - Molecular Biology 2019Quote: ... purified and then cleaved with Xho I and Bam HI (both NEB). After further purification ...
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bioRxiv - Microbiology 2021Quote: ... Then the His-tag was cleaved by incubation with TEV protease (NEB) for 18 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... and then ligated by Hi-Fi DNA assembly (New England Biolabs Inc.). Irgb6-WT and Irgb6-T95D were expressed and purified as described (Saijo-Hamano et ...
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bioRxiv - Biochemistry 2023Quote: ... was cloned into pUC19 by Gibson cloning (Hi-Fi cloning system, NEB) to replace the yeast sequence:
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bioRxiv - Cell Biology 2024Quote: ... and incubated with 200 µM ATP with ot without 1 µl of recombinant PKA catalytic subunit (PKAc; New England Biolabs) in manufacturer-supplied reaction buffer at 30° C for 30min with agitation ...
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bioRxiv - Biophysics 2021Quote: MukF Flag-tagged fragments were expressed from pET 21 plasmids in C3013I cells (NEB).The Flag-tagged MukE and MuF were at the C-terminus ...
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bioRxiv - Cell Biology 2021Quote: ... MBP-tagged fragments were purified by gravity flow through amylose resin (New England Biolabs) and step elution in maltose ...
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bioRxiv - Biochemistry 2023Quote: ... containing a His6-tagged Ulp1 gene was transformed into T7 Express competent cells (NEB) and grown in LB medium supplemented with ampicillin (100 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... MBP-tagged CCDC22 and CCDC93 proteins were purified using Amylose beads (New England Biolabs), mixed in approximately 1:1 stoichiometry ...
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bioRxiv - Microbiology 2020Quote: ... then ligation via T4 DNA ligase (all enzymes from NEB) to yield pCglBWT ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then BspQI restriction enzyme (Cat. # R0712L, New England BioLabs) is added in the reaction system to recognize the stem part of the hairpin and digest the concatemer intermediates into target ssDNA monomers and hairpin adapters ...
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bioRxiv - Genomics 2021Quote: ... Chromatin was digested with 300U DpnII restriction enzyme (NEB, R0543M) in-situ at 37℃ over night with shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... but with 0.5 µL Dam enzyme (New England BioLabs #M0222S) added during the activation step ...
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bioRxiv - Cell Biology 2019Quote: ... All restriction enzymes were purchased from NEB (New England Biolabs). The cript and tbcb gene knock out experiment was performed on HeLa cell lines using CRISPER-Cas9 system with gDNA sequence ...
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bioRxiv - Genomics 2020Quote: ... Digestion was performed overnight using 400U MboI restriction enzyme (NEB). To fillin the generated ends with biotinylated-dATP ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5μL of HindIII restriction enzyme (NEB, Ipswich, MA, USA). Sample DNA was digested at 37°C for 15 minutes and loaded in ddPCR DG8™ Cartridge with QX200™ droplet generation oil (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaned up products were digested using XbaI restriction enzyme (NEB) in 1x CutSmart buffer and run on a 1.5% agarose gel with 1x SYBR Safe (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... and the Uracil-Specific Excision Reagent enzyme (NEB, cat.: M5505L) was used to digest the products.
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bioRxiv - Molecular Biology 2021Quote: ... incubated with 25 units/mL hAAG enzyme (New England Biolabs) at 37°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and then incubated with restriction enzyme mix (HindIII (NEB, R01045), EcoRI (ThermoFisher ...
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bioRxiv - Genomics 2019Quote: ... and labeled using the enzyme Nt.BspQI (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genomics 2019Quote: ... and labeled using the enzyme Nt.BspQI (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2019Quote: ... followed by digestion with DpnI restriction enzyme (New England Biolabs). The constructs H2B-mCherry ...
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bioRxiv - Genomics 2019Quote: ... 20 U of the restriction enzyme BstXI (New England Biolabs) were added directly to the amplification reaction and the mixture was incubated for 3 hours at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... as the primary restriction enzyme and NlaIII (New England Biolabs) as the secondary restriction enzyme ...
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bioRxiv - Genomics 2019Quote: ... Pelleted nuclei were digested with DpnII restriction enzyme (NEB, R0147). The restriction fragment overhangs were filled and marked with biotin-labelled dATP (Thermo Fisher ...
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bioRxiv - Biochemistry 2019Quote: ... restriction enzymes and ligases from New England Biolabs (NEB, UK), Oligonucleotide DNA from Eurofins (India ...
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bioRxiv - Microbiology 2019Quote: ... biprosthecum was digested using restriction enzymes HindIII (New England Biolabs) and EcoRI followed by heat inactivation at 80 °C for 20 min ...
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bioRxiv - Genomics 2019Quote: ... by golden gate ligation using the BsmB1 restriction enzyme (NEB). 293T cells were transfected in DMEM using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... 10 uL of 10U/uL AluI restriction enzyme (NEB R0137S) was added to each sample ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.5 μl of enzyme HpyAV (New England Biolabs, Ipswich, MA), and water to volume ...
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bioRxiv - Genomics 2020Quote: Methylation-sensitive restriction enzyme BstUI (New England Biolabs, Ipswich, USA) was selected based on its capacity to distinguish methylated from unmethylated DNA sequences ...
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bioRxiv - Neuroscience 2020Quote: ... linearized with restriction enzymes NsiI-HF (New England Biolabs #R3127S) and AvrII (New England Biolabs #R0174S).
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bioRxiv - Neuroscience 2020Quote: ... linearized with restriction enzymes NsiI-HF (New England Biolabs #R3127S) and AvrII (New England Biolabs #R0174S).
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bioRxiv - Neuroscience 2020Quote: ... linearized with restriction enzyme NsiI-HF (New England Biolabs #R3127S).
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bioRxiv - Neuroscience 2020Quote: ... linearized with restriction enzymes NsiI-HF (New England Biolabs #R3127S) and AvrII (New England Biolabs #R0174S).
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bioRxiv - Bioengineering 2021Quote: ... Gap-repaired tagmented DNA was treated with USER enzyme (NEB) and T4 polynucleotide kinase (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... The glycosidase reaction was performed with Endo H enzyme (NEB) under non-denaturing conditions following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... two guide RNAs and the Cas9 enzyme (S. pyogenes, NEB) were diluted to a final concentration of 20 ng/µl each in injection buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.02 U/μl Thermolabile USER® II Enzyme (NEB) (0.5 μl/sample volume ...
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bioRxiv - Neuroscience 2020Quote: ... linearized with restriction enzymes NsiI-HF (New England Biolabs #R3127S) and AvrII (New England Biolabs #R0174S).
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bioRxiv - Neuroscience 2021Quote: ... the PCR product was digested with the AflII enzyme (NEB) whose recognition site is at the targeted cut-off site ...
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bioRxiv - Biochemistry 2021Quote: ... Cloning related enzymes were purchased from New England Biolabs (NEB). Zyppy™ Plasmid Miniprep kit and DNA Clean & Concentrator™ kit were purchased from Zymo Research ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 30 units of phu29 DNA polymerase enzyme (New England Biolabs), 1X phi29 buffer (New England Biolabs) ...