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Citations for New England Biolabs :
601 - 650 of 5774 citations for Recombinant Human Endothelin Converting Enzyme 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... After the IVT reaction was complete the DNA template was digested using DNase 1 enzyme (New England Biolabs, UK). The reaction mixture was purified ...
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bioRxiv - Microbiology 2022Quote: ... Point mutations or small deletions in pGM34 and pGM71 (Table 1) were performed using the KLD enzyme mix (NEB)
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bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2 -O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2’-O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Developmental Biology 2023Quote: ... The reporter HLC plasmid (20ng μl-1) was digested by 0.5 U I-Sce enzyme in its adequate digestion buffer (NEB) for 30’ at 37°C prior to injections ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μL of entry vector (backbone), 0.5 μL of type IIs restriction enzyme (BsaI, BsmBI or BbsI-HF) (NEB), 0.5 μL of T4 DNA Ligase (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... aurantiaca DW4/3–1 genomic DNA and cut by restriction enzymes NdeI and HindIII (New England Biolabs, Beverly, USA), and ligated into the corresponding sites of the expression vector pET28c(+ ...
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bioRxiv - Microbiology 2024Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2L-O-methyltransferase (New England Biolabs). The mRNA was purified ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5 µL of uracil DNA glycosylase enzyme (NEB M0280S), 2 µL of 10x uracil DNA glycosylase buffer (NEB M0280S) ...
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bioRxiv - Cell Biology 2020Quote: ... an oligonucleotide was 5’-labeled using PNK enzyme (NEB). The ITS2 probe was previously reported (Donati et al. ...
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bioRxiv - Genetics 2021Quote: ... Restriction enzymes were supplied by New England BioLabs (NEB) and used according to manufacturer instructions.
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bioRxiv - Molecular Biology 2021Quote: ... All other necessary enzymes were also purchased from NEB. Primers were ordered from Eurofins Genomics and Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 units of Vaccinia capping enzyme (New England Biolabs), 100 units of 2’-O-Me-transferase (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... I-SceI enzyme (Cat#R0694S, NEB; 0.375 U/µl). A quartz needle (Cat#QF100-70-10 ...
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bioRxiv - Developmental Biology 2021Quote: ... Recovered DNA was digested with DpnI restriction enzyme (NEB) to destroy unreplicated plasmids and subsequently purified with QIAGEN gel extraction kit.
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bioRxiv - Molecular Biology 2022Quote: All restriction enzymes are ordered from NEB (Massachusetts, U.S.), including BamHI-HF (Cat ...
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bioRxiv - Molecular Biology 2020Quote: Restriction enzymes were purchased from New England Biolabs (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 μL T4 PNK Enzyme (1000 U/μL, NEB), 0.5 μL RNaseOUT (40 U/μL ...
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bioRxiv - Immunology 2019Quote: ... xbaI and xhoI enzymes (New England Biolabs, Ipswich, MA) were used to clone the Trp2 sequence from the pCR™ 2.1-TOPO® TA Cloning® plasmid into the MIP3α-gp100 vaccine plasmid ...
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bioRxiv - Microbiology 2020Quote: ... using BamHI and EcoRI restriction enzymes (New England Biolabs). The correctness of the constructs was verified by DNA sequencing.
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bioRxiv - Molecular Biology 2019Quote: ... vector was treated with CIP enzyme (New England BioLabs) to reduce the chances of phosphorylation of vector ends ...
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bioRxiv - Genetics 2019Quote: ... 640μM SAM and 20U M.Sss1 enzyme (New England Biolabs) at 37°C followed by inactivation (65°C ...
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bioRxiv - Genomics 2019Quote: ... adapter-ligated cDNA was treated with USER enzyme (NEB). Adapter-ligated libraries were then PCR amplified for 10 cycles using NEB index primers compatible with Illumina sequencing ...
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bioRxiv - Molecular Biology 2019Quote: ... All restriction enzymes and NEB5α competent cells (NEB C2987H) are from NEB ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were digested by T7E1 enzyme (NEB), resolved on 2% agarose gel and then analyzed by densitometry measurements as described [63] ...
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bioRxiv - Microbiology 2019Quote: ... Restriction enzymes and T4 DNA ligase (New England Biolabs) were used as recommended by the manufacturer ...
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bioRxiv - Genetics 2019Quote: ... and cut using the restriction enzyme HindIII (NEB, R0104S) to remove the 702-bp fragment from c(3)G creating the c(3)GccΔ1 mutation ...
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bioRxiv - Biochemistry 2021Quote: ... digested with the NdeI and HindIII restriction enzymes (NEB), and ligated (T4 ligase ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.8 U/μl Klenow enzyme (NEB; Cat#: M0210L). The samples were incubated at 37 °C for 75 min with shaking (1,400 rpm) ...
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bioRxiv - Cell Biology 2021Quote: ... with XhoI and SpeI restriction enzymes (New England Biolabs) was used as intermediate ...
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bioRxiv - Cell Biology 2021Quote: ... 600 units of restriction enzyme EcoRI or HindIII (NEB) was used to digest genomic DNAs by incubating at 37°C overnight ...
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bioRxiv - Genomics 2021Quote: ... 1µl of DraI restriction enzyme (New England BioLabs #R0129S), 5µl of DNA template (4ng/µl ...
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bioRxiv - Plant Biology 2021Quote: ... using restriction enzymes and T4 ligase (New England Biolabs).
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA restriction enzyme digests (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA restriction enzyme digests (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2021Quote: ... Then 3 µl USER® Enzyme (New England BioLabs) was used with size-selected ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid ligation was achieved with KLD Enzyme Mix (NEB) and completed plasmids were sequenced to verify correct mutations (Plasmidsaurus).
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bioRxiv - Biophysics 2022Quote: ... with XhoI and NheI restriction enzymes (New England BioLabs). The product was then packaged into phage particles using phage extract (MaxPlax ...
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bioRxiv - Biochemistry 2022Quote: ... pPD95.75-mSc with PstI and BamHI restriction enzymes (NEB), the linearized plasmids were gel-extracted and assembled with PCR- amplified itr-1 promoter using In-fusion cloning.
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bioRxiv - Microbiology 2020Quote: ... and KpnI-HF restriction enzymes (NEB R3101, R3142 respectively), gene was then PCR amplified and purified then cloned into plasmid pLC29144 using In-Fusion HD Cloning plus kit (Takara) ...
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bioRxiv - Developmental Biology 2021Quote: ... including the restriction enzyme digestion (XbaI, New England Biolabs), in vitro transcription (mMESSAGE mMACHINE T3 Transcription Kit ...
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bioRxiv - Developmental Biology 2020Quote: ... Hairpin adaptors were digested using USER enzyme (NEB, M5505S) at 37C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 units of Vaccinia capping enzyme (New England Biolabs), 100 units of 2′-O-Me-transferase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... The amplicons were digested with PacI restriction enzyme (NEB) for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... Restriction enzymes were purchased from NEB (Ipswich, MA, USA). Inserts were generated by PCR amplification with cloning primers from Integrative DNA Technologies (Coralville ...
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bioRxiv - Genetics 2022Quote: ... or restriction enzyme digestion using Mse I (NEB R0525L). Splicing analysis of exon 2 was done using primers in exons 1 and 3 or exons 1 and 7 ...
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bioRxiv - Molecular Biology 2022Quote: ... was digested with NotI-HF and SacII enzymes (NEB) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... linearized with the restriction enzyme PciI (New England Biolabs), and transformed into GCGS0457 via spot transformation as described 19,36 ...
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bioRxiv - Bioengineering 2019Quote: ... after which 3 μL USER enzyme (New England Biolabs) was incubated with size-selected ...