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Citations for New England Biolabs :
7501 - 7550 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... PCR amplified and then pooled into equimolar amounts and further quantified using the NEBNext Library Quant Kit (New England Biolabs). The pooled library was subjected to 50 bp paired-end sequencing on the Illumina NextSeq 2000 platform.
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bioRxiv - Microbiology 2024Quote: ... Libraries were prepared using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England Biolabs; #E6177) per manufacturers protocol with 50-100 ng of DNA input and 5-6 cycles of PCR ...
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bioRxiv - Immunology 2024Quote: ... Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (Cat# E7760L; New England Biolabs). Purified libraries were qualified on an 4150 TapeStation (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA obtained from each sample was subjected to sequencing library construction using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) with NEBNext Poly(A ...
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bioRxiv - Microbiology 2024Quote: ... and processed into a cDNA library for Illumina with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Dual-index primers were conjugated with NEBNext Multiplex Oligos for Illumina (Set1 ...
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bioRxiv - Cell Biology 2024Quote: ... To generate pcDNA5/FRT-Tg-NLuc plasmids the Tg gene was amplified from the pcDNA5/FRT-Tg-FLAG plasmid and assembled with the NLuc fragment using a HiFi DNA assembly kit (New England BioLabs). To generate the respective mutant construct plasmids for A2234D and C1264R Tg ...
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bioRxiv - Molecular Biology 2024Quote: ... On-column DNase I digestion was performed followed by mRNA isolation with the NEBNext Poly (A) mRNA Magnetic Isolation Kit (NEB). Library preparation was done with the Ultra II Directional RNA Library Prep for Illumina following the manufacturer’s manual ...
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bioRxiv - Biochemistry 2024Quote: ... Site-directed mutagenesis was performed using standard PCR-based mutagenic procedures with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... insert and vector bands were separated by agarose gel electrophoresis and purified with the Monarch DNA Gel Extraction Kit obtained from New England Biolabs (NEB). Ligations were done with the Quick LigationTM Kit (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... A sequencing library was generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, 7645). ChIP-seq was carried out on NovaSeq 6000 (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... RNA input was normalized and NGS libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: mRNA was isolated from 6-well plates showing a cell confluency of ∼90% using the Monarch Total RNA Miniprep kit (NEB). siRNA transfection was performed 48 hours prior to RNA isolation using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Overhanging BsWI sites were added to the oligonucleotides for cloning into the dCasRx plasmid with Quick Ligation kit (M2200S, New England Biolabs) (FW ...
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bioRxiv - Molecular Biology 2024Quote: ... 3µl of annealed oligonucleotides were cloned in 50ng of gRNA backbone vector (previously cut with BbsI-HF and gel extracted) using Quick Ligation kit (#M2200S, New England Biolabs) and transformed in NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Microbiology 2024Quote: ... Up to 1ug of RNA is then converted into cDNA using the NEB LunaScript® RT Supermix Kit (NEB #E3010) followed by qPCR using the NEB Luna Universal qPCR Master Mix (NEB #M3003) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA of 2×106 cells was extracted with the Monarch Total RNA Miniprep Kit with “on column” DNase I treatment (NEB). cDNA was generated by using LunaScript RT SuperMix Kit (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were polyA-enriched and directional libraries were prepared using the NEBNext Ultra II Directional Library Prep Kit for Illumina (NEB). Libraries were PE150 sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs) was utilized according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The preparation of sequencing libraries for ribosome profiling was conducted via the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB, #E7300S). Pair-end sequencing reads of size 150 bp were produced for Ribo-seq on the Ilumina Hiseq X-ten system ...
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bioRxiv - Neuroscience 2024Quote: ... Around 5 ng of purified CUT&RUN-enriched DNA was used to prepare NGS libraries using the NEBNext Ultra II DNA Library Prep Kit (NEB) with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... then 40 μL of each sample was used reactions as outlined in the manufacturer’s protocol for denaturing reaction conditions using the Protein Deglycosylation Mix II Kit (New England Biolabs, P6044) both with and without deglycosylase enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was performed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L). The workflow was adapted to generate longer library fragments by reducing the fragmentation time (see manual ...
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bioRxiv - Molecular Biology 2024Quote: ... After washing samples were resuspended in 150 µl RNA cleanup binding buffer of the Monarch RNA Cleanup Kit (NEB, T2030S). Samples were incubated for 5 min at RT on a rotating wheel ...
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bioRxiv - Neuroscience 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Molecular Biology 2024Quote: ... These three DNA fragments were further assembled using the NEBuilder HiFi DNA Assembly cloning kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Molecular Biology 2024Quote: ... Modifications of uORFs or other mutations were performed using the Q5 site-directed mutagenesis kit (Cat. No. E0554S, New England Biolabs) and validated by Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP-seq libraries were generated using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 cycles on a Covaris E220 focused ultrasonicator and were subsequently processed using NEBNext Enzymatic Methyl-Seq kit (NEB #7120) following the manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... a codon-optimized synthetic gene product was synthesized in two overlapping halves by Twist Biosciences and integrated into a vector using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). pNH-TrxT was a gift from Opher Gileadi (Addgene plasmid # 26106 ...
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bioRxiv - Cell Biology 2024Quote: ... The sgRNAs were synthesized by in vitro transcription from DNA oligonucleotide templates using the HiScribe T7 Transcription Kit (New England Biolabs). The transcribed products were subsequently purified using the RNA Clean & Concentrator (ZYMO RESEARCH) ...
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bioRxiv - Cancer Biology 2024Quote: ... Guide RNAs were generated in house using Highscribe™ T7 Quick High Yield RNA synthesis kit (New England Biolabs Inc.), Alt-R™ S.p ...
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bioRxiv - Pathology 2024Quote: ... cDNA library was generated from total mRNA (1 µg) using NEBNext UltraTM RNA Library Prep Kits for Illumina (New England BioLabs). cDNA libraries were sequenced by NovaSeq 6000 (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... graminearum or mock inoculated was treated with DNAse and cDNA was synthesized using LunaSCript RT MasterMix kit (New England Biolabs), with random hexamer and oligo-dT primers ...
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bioRxiv - Neuroscience 2024Quote: ... The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Physiology 2024Quote: ... DNA libraries were generated as per the manufacturer’s protocol using the NEBNext® Poly(A) mRNA Magnetic Isolation Module kit (New England Biolabs). 1μg total RNA per sample was inputted and adaptor ligation and PCR enrichment was performed using the NEBNext® Multiplex Oligos for Illumina® kit as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... IVT was performed following the manufacturer’s protocol for the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA). Polyadenylated RNA was added to the IVT RNA control at a ratio of 1:1000.
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bioRxiv - Neuroscience 2024Quote: ... The four separate fragments were amplified and inserted into a vector containing the Tol2 sites35 using the Gibson Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and then adenylated at the 5′ end with a 5′ DNA adenylation kit (NEB). Four random nucleotides were added in the 3′ and 5′ adapters [(5′ -rAppNNNNTGGAATTCTCGGGTGCCAAGG/amino CA linker-3′ ...
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bioRxiv - Biochemistry 2024Quote: ... 1-5 mL of cell pellet was harvested for plasmid extraction with the Monarch Plasmid DNA Miniprep Kit (New England Biolabs) (T1010) ...
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bioRxiv - Biochemistry 2024Quote: ... with C-terminal TEV, GFP, 7xHis, and StrepII (Thawani et al., 2020) using the Q5 Site Directed Mutagenesis Kit (NEB). All three constructs were expressed using the Bac-to-Bac Sf9 cell expression system (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Non-Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit (dsDNA HS kit ...
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bioRxiv - Developmental Biology 2024Quote: ... Construction of pUAST- attB-Cic(1-15)A-SBP and pUAST-attB-Cic20A-mVenus was carried out by assembling gene fragments using the HiFi DNA assembly Kit (NEB). Construction of the other subgroups of Cic mutants was done by assembling different parts of Cic generated by overlap PCR using wild type Cic ...
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bioRxiv - Neuroscience 2024Quote: ... were cloned N-terminally to human CISD1 and point mutations introduced by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis kit (NEB). All plasmids were sequence verified ...
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bioRxiv - Biochemistry 2024Quote: ... was introduced into linearized pEGFP-C1 ΔSV40-Stop with Xho I and BamH I use the HiFi DNA Assembly Cloning Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ng of ChIP and Input DNA was used to generate ChIP-seq libraries using the NEBNext Ultra DNA library prep kit for Illumina (New England Biolabs, NEB).
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bioRxiv - Genomics 2024Quote: ... Following end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA), custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix (Cat No ...
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bioRxiv - Biochemistry 2024Quote: ... Of the rRNA-depleted samples directional libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) in accordance to the recommended protocol ...
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bioRxiv - Biophysics 2024Quote: ... Plasmid DNA was extracted using the Qiagen Plasmid Plus Midi kit and the library inserts were obtained through double endonuclease digestion (HindIII-HF and NheI-HF, NEB) followed by agarose gel purification achieved by a combined use of centrifugal filter units (Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... Lec5 and Lec9 CHO cells was extracted using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, T3050L). Resulting gDNA was then sheared by passing each sample 25 times through a 26G needle ...