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Citations for New England Biolabs :
7751 - 7800 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... The samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with an additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Genetics 2020Quote: ... Followed by a Dual index library prep with the DNA NEBNext® Ultra™ II Library Prep Kit (New England Biolabs), using a quarter of the recommended volumes with the Dual Index Primers Set ...
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bioRxiv - Genetics 2021Quote: ... Libraries for sequencing were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645), as described by the manufacturer but using half volumes of all reagents and starting material ...
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bioRxiv - Microbiology 2021Quote: ... Mutant of the dGTPase gene of Shewanella putrefaciens CN-32 was constructed using Q5 Site directed Mutagenesis kit (NEB, cat. #E0554S) using primers presented in Supplementary Table S5.
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bioRxiv - Microbiology 2020Quote: ... qRTPCR was performed with primers mentioned in Table S3 with Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs). Log2 normalized RNA abundances were calculated using ValidPrime signal as reference (51).
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bioRxiv - Microbiology 2021Quote: ... and fifteen repeats of the FG-repeats were amplified from genomic DNA using primers (Table S2) with Phusion High-Fidelity PCR Kit (NEB - E0553S). The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020) ...
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bioRxiv - Microbiology 2021Quote: ... while the Zymo Mock sample was prepared with NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs, USA) following the manufacturer’s protocols and sequenced using the Illumina MiSeq platform.
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed either directly on the inactivated supernatants or on extracted RNA using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005E) in a QuantStudio 6 thermocycler (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: The gene encoding the C-terminal 6xHis GlcNAc-1-kinase NahK from Bifodobacterium longum JCM1217 was inserted into a pBAD/HisB backbone using a NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs) following manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Double-stranded single-indexed libraries were prepared using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645S) and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 and 2 (NEB #E7335S ...
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bioRxiv - Microbiology 2021Quote: ... and all of the point mutants were constructed using Mut Express II Fast Mutagenesis Kit V2 (Vazyme, C214) and DpnI endonuclease (NEB, R0176S). Primers used for plasmids construction were listed in Supplementary table 1 and table 2 ...
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bioRxiv - Microbiology 2020Quote: ... A band at approximately 1 kb was cut out and DNA extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the sgRNA cassette from lentiCRISPRv2 was removed by digestion with KpnI and EcoRI and the plasmid re-ligated using annealed repair oligo pair 1 (see Table S3) and Quick Ligation™ Kit (M2200L, NEB). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-miRNAs were purified on denaturant polyacrylamide gel and long pri-miR-K10/12 derived transcripts (up to ∼3 kb) were salt purified using Monarch® PCR and DNA cleanup kit (New England BioLabs). After acidic phenol extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... EF1α Lamp1-mCherry plasmid was extracted from successful transformants with the Monarch® Plasmid Miniprep Kit (New England BioLabs Inc., USA), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... was used to generate RNA-seq library using NEBNext Ultra Directional RNA library Prep Kit for Illumina (New England BioLabs, Inc) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... were used to construct the RNA-seq libraries using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Entry vectors for Golden Gate cloning were made by digesting the Greengate Kit [56] entry vector plasmids (PGGA000, PGGB000, PGGC000, PGGD000, PGGE000, PGGF000) with BSA1-HF V2 (New England Biolabs Inc.). Bladderwort sequences were synthesized (IDT ...
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bioRxiv - Plant Biology 2021Quote: ... The pooled PCR reactions were purified with the Monarch® PCR & DNA Cleanup Kit (5 μg) (New England Biolabs® Inc.) and run on 6% acrylamide gel ...
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bioRxiv - Synthetic Biology 2021Quote: ... All the three fragments were assembled uisng the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, Ipswitch, MA, USA) and transformed into E ...
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bioRxiv - Systems Biology 2021Quote: ... Sequencing libraries were generated from the qualified DNA samples(s) using the NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated following manufacturer’s recommendations with NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA). The constructed libraries were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... (Leiden, The Netherlands) using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) and the NextSeq 500 platform (paired-end ...
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bioRxiv - Molecular Biology 2020Quote: ... ATG-free versions of the truncated OpIE2 promoter sequence (tIE2 promoter with no ATG triplet in its sequence) were created by Q5 Site-Directed Mutagenesis (SDM) Kit (New England Biolabs-NEB, USA). Primers used to generate mutations were designed using the NEBaseChanger tool (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... to lysine (K) at 363 (E363K) mutant of HNF4A2 was generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The pcDNA3-SHP-Flag vector for SHP was synthesized by GenScript ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently purified by extraction from a 1% agarose gel using the Monarch DNA Gel Extraction Kit following manufacturer instructions (NEB # T1020S). The partially degenerate DNA oligonucleotide ...
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bioRxiv - Microbiology 2020Quote: ... and libraries for RNAseq were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The 5’ arm and LexA:p65 coding sequence was assembled from 2 fragments from PCR-amplified Drosophila genome and a LexA:p65 coding sequence using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, cat#E5520), and inserted into XhoI-SpeI sites of the pHD-DsRed plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... and gene expression was analyzed by RT-qPCR on QuantStudio 6 (Applied Biosciences) using the Luna Universal qPCR kit (New England Biolabs, M3003). Relative expression was normalized to Actb and Gapdh housekeeping genes and was determined using the ΔΔCt method ...
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bioRxiv - Cancer Biology 2020Quote: ... The GST tag in this construct was replaced by a 6xHis tag using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). N-PTEN was recombinantly expressed in E ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng total RNA per 20 µl reaction were analyzed using the Luna Universal Probe One-Step RT-qPCR Kit (NEB, # E3006) in technical triplicates ...
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bioRxiv - Cell Biology 2020Quote: ... The pPISD-LD-eGFP vector was used as substrate to generate different mutants of PISD-LD by Q5® Site-Directed Mutagenesis kit (NEB). All final PCR-generated DNAs were confirmed by DNA sequencing ...
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bioRxiv - Genetics 2021Quote: ... cDNA was generated from 1ug of total RNA using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, E6560). RT-qPCR analysis was performed in Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... The pair-end sequencing libraries with ∼300 bp insert size were constructed following the instructions of NEB Next Ultra DNA Library Prep Kit for Illumina (E7370, New England Biolabs, USA). Illumina HiSeq 2500 systems were used for sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB, USA). Sequencing was performed using a NextSeq HIGH 150 (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: Libraries were prepared using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs). Quality control and quantification of the libraries were performed using the Bioanalyzer DNA 1000 kit (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... was used per sample for the library preparation using the NEB Next Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7760L). The libraries were then sequenced on Illumina HiSeq 2500 sequencing platform using 2×100 bp sequencing format.
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bioRxiv - Biochemistry 2022Quote: ... UPF2L (120-1227) were produced by PCR amplification (primers listed in Supplementary Table S1) using the Phusion High-Fidelity PCR kit (NEB #E0553S) prior to restriction digest and ligation into pPROEX-HTB (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Y160D+Y167D and Avi-tagged Avi-UPF2-MIF4GIII as well as Avi-UPF3B-WT constructs were generated using a Q5 Site-Directed Mutagenesis Kit (NEB #E0554S) with mutagenic primers (Eurofins Genomics ...
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bioRxiv - Molecular Biology 2022Quote: The rest of RNAs were performed with real-time reverse transcription PCR (RT-qPCR) using a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s recommendations by a thermocycler (BIO-RAD CFX96™ Real-Time System) ...
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bioRxiv - Genomics 2022Quote: ... The resulting polyA-selected RNA was used to prepare RNA-seq libraries using the NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (cat. #E7420L, New England Biolabs), NEBNext Multiplex Oligos for Illumina® Dual Index Primers (cat ...
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bioRxiv - Cell Biology 2022Quote: RNA isolation was performed starting with a confluent 10cm dish and using the Monarch total RNA miniprep kit for RNA isolation (NEB, #T2010S). RNA was stored at −80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Piggybac vectors containing FLAG-BAF WT and FLAG BAF A12T were subsequently assembled by PCR amplification followed by Gibson assembly using HiFi DNA assembly cloning kit (New England Biolabs, #E5520S). FLAG-BAF was cloned into a BamHI and AfeI digested piggybacV1_CMV ...
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bioRxiv - Molecular Biology 2022Quote: ... all of the eluted DNA was used to construct NextSeq DNA libraries using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, #E7645S). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (New England Biolabs (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... The library was cloned into a pENTR1A plasmid backbone with a C-terminal YFP HA reporter tag using Gibson Assembly (HiFi DNA Assembly Cloning Kit, New England Biolabs, (NEB), Ipswich ...
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bioRxiv - Biochemistry 2022Quote: ... the corresponding single and multiple amino acid substitutions were introduced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Cancer Biology 2022Quote: ... Fragmented DNA was end-repaired and adapter ligation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA) for Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... The genomic DNA was subjected to a 5-hmC-specific enrichment with EpiMark 5-hmC and 5-mC analysis kit (New England Biolabs, USA). The processed DNA samples were analyzed with qPCR using loci-specific primers (Sf 4) ...
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bioRxiv - Molecular Biology 2022Quote: ... strand specific RNA-seq libraries were generated using NEBNext polyA selection and Ultra Directional RNA library preparation kits (NEB, Hertfordshire, UK), multiplexed ...
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bioRxiv - Plant Biology 2022Quote: ... RAPTOR1B full-length cDNA was cloned into pENTR4 and used for generating site-mutated constructs with the Q5 site-directed mutagenesis kit (New England Biolabs, E0554S). RAPTOR1B full-length and site-mutated cDNA were subcloned into binary vector pGWB611 by Gateway LR recombination reaction (Invitrogen ...