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Citations for New England Biolabs :
701 - 750 of 3976 citations for Red colored Polystyrene Beads 1 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... mRNA was enriched with oligo(dT)-beads and cDNA was synthesized with random hexamer primers with the NEB Next Ultra RNA Library Prep Kit (NEB, Ipswich, USA). All libraries were sequenced using the Illumina NovaSeq6000 platform in paired-end mode with a read length of 150 bp.
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was discarded by isolating the magnetic beads with a magnetic rack (NEBNext® Magnetic Separation Rack, New England BioLabs, Inc.). The collateral cleaved products by CRISPR were then added to the magnetic beads at room temperature for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were then re-suspended in 23 μL of 10 mM Tris-Cl pH 8.0 and libraries were prepared by on-bead reactions using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand and the supernatant was discarded ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation experiments were performed with 15 μL of Pan anti-glycine lysine antibody conjugated to agarose beads (PTM Biolabs, Chicago, IL, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA-Seq libraries were prepared from these enriched samples and from unenriched control “gold standard” samples (i.e. “Unenriched”) using the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB #E7765) and NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1 ...
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bioRxiv - Genomics 2021Quote: ... and end-repair processing using the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs, catalog no. E7775L). Adapters and unique dual indexes in the NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed 2 times with EB2 without NaF or PhosStop and one time with λpp buffer (New England Biolabs, Ipswich, MA) before resuspension in 40 μl of λpp buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to an input of 150 ng before preparation with a NEBNext® Ultra™ II RNA Library Prep (Magnetic Bead) kit (New England Biolabs). The polyA mRNA protocol was used for all preps ...
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bioRxiv - Developmental Biology 2024Quote: Sequencing libraries were prepared using a commercial kit (NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads, New England Biolabs, Ipswich, USA), and cDNA of three replicates per strain was sequenced as 150 bp (100 bp for Mpyr_CVe13f ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA libraries were constructed using a NEBNext Ultra II RNA Library Prep with Sample Purification Beads (Code E7775; New England Biolabs, Tokyo, Japan), a NEBNext Poly(A ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was extracted from homogenized cell lysate by 4 × 250 µL/brain Oligo (dT)25 magnetic beads (New England Biolabs, Cat: S1419S), and immunoprecipitated (IP ...
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bioRxiv - Genomics 2024Quote: ... 120 μg of total liver RNA was isolated from 60-100 mg liver tissue from each HDI-STARR-seq-treated mouse by TRIzol guanidinium thiocyanate-phenol-chloroform extraction then purified by polyA selection using oligo(dT) beads (New England Biolabs, cat. #E7490L). The purified RNA was digested with Turbo DNase-I (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... and urea buffers were conducted according to Gu et al. (Gu et al. 2018) On-bead digestion was performed by adding 100 µL Proteinase K (New England Biolabs, cat # P8107S) followed by incubation at 37°C for 75 min ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted using NEBNext® rRNA Depletion Kit (Bacteria) and NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads (New England Biolabs, UK). An equimolar mixture of depletion solutions from each kit was used during the hybridization step to deplete both eukaryotic and prokaryotic rRNA simultaneously ...
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bioRxiv - Genomics 2024Quote: ... Beads were resuspended in 50 µl of TE buffer and libraries were made on beads using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB # E7645S) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... HiFi products for each library were cleaned with Ampure XP beads and transformed into 10-beta electrocompetent cells (NEB, Ref. No. C3020K) with a MicroPulser Electroporator (BioRad ...
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bioRxiv - Genomics 2024Quote: Magnetic bead clean-up was performed for each pool separately using NEBNext Ultra II DNA Library Prep with Sample Purification Beads (New England Biolabs Inc., UK). The clean-up was performed at a dilution of 0.9x ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA libraries were constructed using a NEBNext Ultra II RNA Library Prep Kit with Sample Purification Beads (New England Biolabs, Tokyo, Japan), a NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2024Quote: ... The library prep was performed with the samples on beads using the NEBNext® Ultra II Directional DNA Library Prep Kit (NEB E7645), with several modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... the beads were re-suspended in a 19 μL reaction buffer containing 2 μL 10× T4 DNA ligase buffer (NEB, Cat. B0202S) and 1 μL USER Enzyme (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The immunoprecipitated DNA was size-selected again using AMPure XP beads and amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Catalog #M0491). The amplification products were purified with AMPure XP beads ...
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bioRxiv - Genetics 2024Quote: ... 500 ng to 1 µg of total RNA was converted to Illumina sequencing libraries using the NEB Ultra II Directional RNA Library Prep kit with Sample Purification Beads (NEB cat. #E7765), with 10-14 PCR cycles (determined empirically to achieve the minimum number of cycles per library) ...
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bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...
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bioRxiv - Genomics 2022Quote: ... The extracted RNA was used for library preparation (NEBNext Ultra II RNA Library Prep with Sample Purification Beads, #E7775S) with appropriate barcoded sequencing primers (NEB #E7335-12 indices).
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bioRxiv - Genetics 2022Quote: ... and end-repair processing were performed with the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs, catalog no. E7775L). The cDNA libraries were adapter-ligated using adapters and unique dual indexes in the NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.01% Tween] to 3 µM concentration in a total volume of 50 µl and mixed with 20 μl of amylose beads (New England Biolabs, Ipswich, US-MA). After mixing the proteins and the beads ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (E7765S, New England Biolabs, Ipswich, MA, USA), NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2021Quote: ... The mixture containing RBBP6/CPSF/RBBP6+CPSF and MBP-MS2-bound L3 pre-mRNA was mixed with amylose beads (NEB, cat. No. E8021) equilibrated in pull-down buffer and incubated rotating at 4°C for 1.5 h ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries were equimolarly pooled in batches of ∼15 (five libraries per age cohort) and cleaned using an equal volume of NEBNext® Sample Purification Beads (New England BioLabs® Inc.). The library pools were eluted in 25 μL TE buffer (1 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... and the enriched mRNA was prepped for sequencing with the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB, #E7765S) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified IP products were taken for library preparation using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (NEB, E7103L) as per manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and end-repair processing was performed using the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs, catalog no. E7775L). We ligated adapters in the cDNA library using adapters and unique dual indexes from the NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Then total RNA (500 ng per sample) was purified by one round of polyA mRNA selection with oligo-dT magnetic beads (NEB, Cat. No. S1550S), converted to cDNA using KAPA mRNA HyperPrep kit (KAPA biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA libraries were constructed using a NEBNext Ultra II RNA Library Prep with Sample Purification Beads (E7775S, New England Biolabs, Ipswich, MA, USA), a NEBNext Poly(A ...
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bioRxiv - Neuroscience 2024Quote: ... The rRNA-depleted RNA was converted [15] into Illumina-compatible DNA libraries via the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads kit (NEB# E7765) using the manufacturer’s protocol for use with rRNA-depleted RNA and NEBNext® Multiplex Oligos for Illumina® (NEB# E6448) ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of Exonuclease 1 (NEB, M0293S) was added to each PCR product and incubated at 37C for 15 min to digest any single-stranded product ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× NEB buffer 1 (NEB, B7001S) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1% cholorophorm was added together with 10 μg mL-1 DNase 1 (NEB) and 1 μg mL-1 RNase A ...
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bioRxiv - Molecular Biology 2024Quote: ... The four PCR products were then assembled in a 1:1:1:1 ratio using HiFi assembly (NEB, E5520S) according to the manufacturer’s instructions to create the pLenti-STARR vector (pAS5018) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The described method follows the NEBNext® Ultra™ II DNA Library Prep Kit (v4.0) with Sample Purification Beads Kit (New England Biolabs, Ipswich, Massachusetts, USA). But other kits can be used too ...
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bioRxiv - Microbiology 2021Quote: ... MNase activity was quenched by addition of EGTA and samples were subsequently pre-cleared by addition of 40 µl Protein A Magnetic Beads (New England Biolabs, Ipswich, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Microbiology 2020Quote: ... samples were treated using a Pan-Prokaryote riboPOOL™ kit (siTOOLs Biotech Gmbh, Planegg, Germany) coupled with streptavidin magnetic beads (New England Biolabs, Ipswich, MA, USA). mRNA was eluted in 50 μl of nuclease-free water and then cleaned and concentrated using a Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: The recombinant constructs (pETDuet-1-TaPHB-1 and pETDuet-1-TaPHB-1-RUVBL1 were separately transformed into E. coli Lemo21(DE3) (NEB) chemical competent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Unincorporated ddRTP molecules were inactivated by adding 1 µL of Buffer 2 (80 mM MgCl2) and 1 µL of 1:1 rSAP (NEB):50% glycerol ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μL T4 RNA Ligase 1 (NEB, M0437M), 1 μL SuperAseIn (Invitrogen AM2694) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM MgCl2) supplemented with 1 mM ATP and 1 U/μL RNase Inhibitor (NEB). Reaction was incubated for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were diluted 1:1 and 1:5 respectively in puc19 vector (New England Biolabs). HaloTag expression was confirmed using HaloTag ligand dyes HTL-AF488 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 unit µL-1 EcoRI-HF (New England Biolabs)) ...
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bioRxiv - Biophysics 2020Quote: ... 1 U μL−1 murine RNase inhibitor (NEB, M0314L), 600 ng mRNA and 50 μM PF846 in 1% DMSO ...
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bioRxiv - Genomics 2021Quote: ... 1 unit/μl RNA ligase 1 (New England Biolabs), 1× RNA ligase buffer ...