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Citations for New England Biolabs :
551 - 600 of 3976 citations for Red colored Polystyrene Beads 1 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The beads were covered with 30 μL of reverse transcription mixture containing dNTPs (0.5 mM each, New England BioLabs), acetylated BSA (1,5 μg) ...
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bioRxiv - Biochemistry 2022Quote: ... and the poly(A) RNA was isolated with two rounds of selection using oligo-(dT)25 beads (NEB, S1419S). The purified poly(A ...
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bioRxiv - Molecular Biology 2024Quote: ... a master mix containing hydrogel beads carrying DNA stub and ligation mixture was distributed in the same 4 x 96-well plates such that each well would contain 12.5 μl of close-packed hydrogel beads,1.25 µl of 10× ligation buffer (NEB, B0202S), 0.3 μl of T4 DNA ligase I (NEB ...
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bioRxiv - Genomics 2023Quote: ... The poly-A+ fraction of RNA was isolated using Oligo d(T)25 Magnetic Beads (New England BioLabs Inc.) following the commercial protocol for Mammalian Cells provided by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared by on-bead reactions using the NEB Next Ultra II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼40 µg of bulk RNA was subjected to poly(A) selection using Oligo d(T)25 magnetic beads (NEB) followed by on-bead 5’ ligation of a biotinylated REL5 linker sequence using T4 RNA ligase 1 (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was then end-repaired and poly(A) tailed on-bead by treatment first with T4 PNK (NEB) and then treatment with yeast poly(A ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were transferred to a tube and incubated with 10 μM SNAP-Cell TMR-Star dye (New England Biolabs) for 1 hour at 4°C prior to the TEV buffer washing step ...
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bioRxiv - Molecular Biology 2023Quote: ... the product was purified using magnetic SPRI beads and amplified using 0.5 µM i5/i7 Illumina indexed primers in Q5 High-Fidelity Master Mix (NEB).
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bioRxiv - Cell Biology 2023Quote: ... We prepared libraries using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (New England BioLabs). We indexed samples using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3 & Index Primers Set 4) ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was constructed with Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB E7765) and sequenced on sequencing machine ...
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bioRxiv - Neuroscience 2023Quote: ... RNAs were eluted by mixing the beads with 21µl RNase H Elution Buffer and 4 µl RNase H (New England Biolabs) and incubated at 37 °C for 30 min while shaking ...
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bioRxiv - Genetics 2023Quote: Libraries in this paper were prepared using NEB’s rRNA Depletion Kit (Bacteria) for rRNA removal with beads (NEB #E7860). Post rRNA removal samples were prepared for either Illumina or Singular Sequencing using NEBNext Ultra II RNA Library Prep Kit for Illumina with Beads (NEB#E7775) ...
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bioRxiv - Biochemistry 2024Quote: ... they were centrifuged for 15 min at 16,000 xg prior to mixing them with 25 µl of amylose beads (New England Biolabs) or glutathione beads (Serva) ...
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bioRxiv - Molecular Biology 2024Quote: ... A customized linker (rand10_3Tr3) was added to the 3’ end of on-bead LAM products using T4 RNA ligase I (M0204S, NEB). Nested PCR was performed using indicated primer set (3Tr3-Adaptor_R and JM_F ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were resuspended in 2X B&W buffer supplemented with 0.4 U/µL RNase Inhibitor (Murine) (NEB, M0314S) to a bead concentration of 5 ug/µL ...
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bioRxiv - Cell Biology 2024Quote: ... The pooled library was once again bead purified and quantified by qPCR using NEBNext Library Quant Kit (NEB, E7630). The library size was verified using a High Sensitivity DNA Kit on a 2100AB Bioanalyzer instrument (Agilent) ...
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bioRxiv - Systems Biology 2024Quote: ... Linear barcoded vectors were purified by magnetic beads and digested for 3h with 40 units of XcmI (#R0533S; NEB). Finally barcoded vectors were purified again by gel-purification.
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bioRxiv - Plant Biology 2024Quote: ... The beads were resuspended in 30 µl of CIP solution (7.5 U of Quick CIP (M0525L, New England Biolabs) in 30 µl of 1× rCutSmart Buffer (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was then extracted from washed beads using Trizol LS and treated with T4 Polynucleotide Kinase (New England Biolabs) to obtain 5’ phosphate ends for subsequent ligations and passed through NucAway columns (Ambion ...
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bioRxiv - Developmental Biology 2024Quote: Polyadenylated RNA was purified from the DNase-treated total RNA using oligo d(T)25 magnetic beads (NEB, S1419), and then used for the library preparation ...
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bioRxiv - Genomics 2024Quote: ... Supernatant containing 200-500 bp DNA sizes was separated from beads using 12-tube magnetic separation rack (NEB # S1509S) and collected ...
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bioRxiv - Biophysics 2024Quote: ... DNA was purified using SPRI beads and subjected to a second restriction enzyme digestion using NlaIII (NEB, Cat. R0125L) to generate sticky ends for circularization ...
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bioRxiv - Genomics 2024Quote: ... beads were resuspended in 55 μL PCR Mix (1X Q5 Hot Start High-Fidelity 2X Master Mix (NEB M0494L), 0.45 μM PCR_TSO ...
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bioRxiv - Cell Biology 2024Quote: ... beads were transferred to a tube and incubated with 10 μM SNAP-Cell TMR-Star dye (New England Biolabs) for 1 hour at 4°C prior to the TEV buffer washing step ...
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bioRxiv - Genetics 2024Quote: ... chromatin was diluted with a lysis buffer up to 0.1-0.2% SDS and pre-cleared by incubation with Protein A magnetic beads (New England Biolabs, S1425S) for 2h at 4C with slow rotation ...
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bioRxiv - Plant Biology 2020Quote: ... captured messenger RNA was isolated from the lysate by adding 20 µL of LBB-washed streptavidin-coated magnetic beads (New England Biolabs) and was allowed to stand for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... to 3 μM concentration in a total volume of 50 μl and mixed with 20 μl amylose beads (New England BioLabs). After mixing the proteins and the beads ...
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bioRxiv - Cancer Biology 2021Quote: ... phosphorylated and bead-purified in preparation for A-tailing using a custom NEB Paired-End Sample Prep Premix Kit (New England Biolabs). Illumina sequencing adapters were ligated for 15 minutes at 20°C and adapter-ligated products were then bead-purified and enriched with 10 cycles of PCR using primers containing a hexamer index that enables library pooling.
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bioRxiv - Cell Biology 2020Quote: ... Beads were resuspended in H2O used for library preparation with the NEBNext Ultra II Library Prep kit for Illumina (NEB). Beads were then washed 4 times using Tween wash buffer (5 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and end-repair processing were performed with the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs).
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bioRxiv - Developmental Biology 2020Quote: 500 ng of DNA attached to beads were end-repaired by incubating in 100 μl end-repair mix (1x T4 Ligase Buffer (NEB), 0.5 mM dNTP mix ...
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bioRxiv - Microbiology 2020Quote: ... glabrata total cell lysates using the anti-Rad53 antibodies described in the previous section and Protein A magnetic beads (New England Biolabs). The immunoprecipitated samples were run on 8% acrylamide gels ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were subjected to glass bead lysis in the presence of 1x phosphatase buffer mix (1x PMP buffer (New England Biolabs), 1x MnCl2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were resuspended in Elution Buffer (Wash Buffer supplemented with TEV protease and Murine RNase Inhibitor (New England Biolabs)) and the bound Enp1-TAP containing complexes were eluted for 1.5 – 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (3 μg) was applied for poly(A) mRNA purification by using oligo-d(T) magnetic beads (S1419S, NEB). RNA fragmentation ...
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bioRxiv - Immunology 2022Quote: ... the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 100ng total RNA (with the Magnetic mRNA Isolation Kit from NEB), a fragmentation using divalent cations under elevated temperature to obtain approximately 300bp pieces ...
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bioRxiv - Genomics 2021Quote: ... end repair was carried out with a mixture of bead-immobilized T4 DNA polymerase and T4 polynucleotide kinase (both kind gifts of Dr. M. Xu, New England Biolabs) in a 20 µl volume of End Repair buffer ...
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bioRxiv - Genomics 2021Quote: ... Libraries were purified with SPRI beads (0.9X) and quantified on a bioanalyzer and with NEB Illumina quant kit (NEB#E7630). HiC libraries were sequenced on a NextSeq500 either 150bp or 75bp paired-end reads.
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bioRxiv - Biochemistry 2020Quote: ... These beads were then resuspended in equal amounts of PBS+ DNaseI digestion buffer with 3 ul (6U) of DNAseI (M0303S, NEB) and incubated at 37°C for 40min - 1h and separated by centrifuging at 2000 rpm for 2 min ...
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bioRxiv - Immunology 2021Quote: ... IDEZ was used to cleave the Fab (in the supernatant) from the Fc (remained on the magnetic beads) (New England Biolabs) for 1 h at 37°C and collected ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was collected after a spin at 16000 g for 30 min and incubated with GFP-Trap (Chromtek) or MBP magnetic beads (NEB) for 3 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: Protein bound glutathione Sepharose beads were resuspended in 1x NEBuffer™ for Protein Kinases supplemented with 1mM ATPγS and 10 units of CK2 (NEB) and incubated at 30°C for 45 minutes ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from HEK293T cells using Bio Tri RNA reagent (Bio-lab) and mRNA was captured using Oligo d(T)25 magnetic Beads (NEB) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Then we performed the bead-on T7 transcription reaction by HiScribe™ T7 High Yield RNA Synthesis Kit (NEB E2040S). The amplified RNAs were processed to the PCR-cDNA sequencing kit (nanopore) ...
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bioRxiv - Biophysics 2022Quote: ... GephE and its dimer-deficient mutant (GephEmm) were initially subjected to affinity chromatography on chitin agarose beads (New England Biolabs), followed by ion exchange chromatography and subsequent size exclusion chromatography.
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bioRxiv - Neuroscience 2020Quote: ... Equal volumes of each immunoprecipitated sample were then aliquoted into two tubes and beads were then resuspended with 100 microliters of lambda phosphatase assay buffer containing 400 units of lambda protein phosphatase (NEB), or with 100 microliters of lambda phosphatase assay buffer alone ...
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bioRxiv - Plant Biology 2021Quote: ... captured messenger RNA was isolated from the lysate by adding 20 μl of LBB-washed streptavidin-coated magnetic beads (New England Biolabs) and incubated for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... the ligated DNA was subjected to a Fill-in reaction by resuspending the beads in 40 μL of 1X phi29 buffer (NEB) containing 0.2 mg/mL BSA (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was subjected to λ exonuclease digestion by incubating the beads with 50 μL of λ exonuclease reaction buffer (NEB) containing 0.1% Triton X-100 ...